RchiOBHm_Chr1g0347741

Anticodon-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001597
Physical Location & Seq
Forward (+)
40418814 .. 40419812
999 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 438 bp
ATGGCCATGGATGGCAGCAATGCAGCAGCAACGGAGGAATTGGCCGTAGGTTGCTTCGTCTCCATCAAGACCACCTTGGGGGACGACTTCCAGGGCCAGGTCATCACCTTCGACCGCCCCTCCAACATCCTCATCCTTCAAGAGGGTTTGAAAGGAGGGCCTAAGCGGAACATCAGGCTACTCAAGGCCAACTATATCAATGAGTTGAGCTATTTGGGTCAAGCTGAAGACCCACTTGACATTAAGAACTGTTACCTTGATCTTAATAGTCTCAGAGCCAGAGATACTCAGGCAGAGTGTGAGAGGATCGGAGTTAGTGTCACCAGCCAGGCTCAGAACATTTTCGATGCCTTGTCTAAGACGCTTCCAGTACGCTGGGACAAGACTGTCATAGTTGTGATGAATGAGGTTCGTGTGAGCAGTCCATATCTTCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AD

Protein Analysis

145

Amino Acids

15.99

Weight (kDa)

4.96

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM12_LSM PF21166 12 - 67 3.7e-13 LSM12, LSM domain
AD PF09793 94 - 143 8.5e-17 Anticodon-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 386
AciI CCGC 2 cut(s) 115, 166
AclWI GGATC 1 cut(s) 314
AcoI YGGCCR 2 cut(s) 3, 42
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 1 cut(s) 372
AfiI CCNNNNNNNGG 3 cut(s) 78, 97, 142
AgsI TTSAA 2 cut(s) 140, 151
AjnI CCWGG 3 cut(s) 90, 96, 327
AluBI AGCT 2 cut(s) 210, 224
AluI AGCT 2 cut(s) 210, 224
Alw26I GTCTC 2 cut(s) 64, 275
AlwI GGATC 1 cut(s) 314
AoxI GGCC 5 cut(s) 3, 42, 94, 158, 186
ApeKI GCWGC 3 cut(s) 15, 23, 26
Asp700I GAANNNNTTC 1 cut(s) 341
AspS9I GGNCC 2 cut(s) 94, 158
AsuHPI GGTGA 2 cut(s) 97, 313
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 1 cut(s) 234
BbvI GCAGC 3 cut(s) 27, 35, 38
BccI CCATC 2 cut(s) 5, 71
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 3 cut(s) 92, 98, 329
BcoDI GTCTC 2 cut(s) 64, 275
BfaI CTAG 1 cut(s) 436
BisI GCNGC 3 cut(s) 16, 24, 27
BlsI GCNGC 3 cut(s) 17, 25, 28
Bme1390I CCNGG 3 cut(s) 92, 98, 329
BmgT120I GGNCC 2 cut(s) 94, 158
BmrFI CCNGG 3 cut(s) 92, 98, 329
BmsI GCATC 1 cut(s) 337
BpiI GAAGAC 1 cut(s) 234
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 1 cut(s) 167
BsaJI CCNNGG 3 cut(s) 6, 75, 91
BsaXI ACNNNNNCTCC 4 cut(s) 104, 134, 303, 333
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 97, 142
Bse1I ACTGG 1 cut(s) 368
Bse3DI GCAATG 1 cut(s) 25
BseBI CCWGG 3 cut(s) 92, 98, 329
BseDI CCNNGG 3 cut(s) 6, 75, 91
BseGI GGATG 3 cut(s) 16, 126, 132
BseLI CCNNNNNNNGG 3 cut(s) 78, 97, 142
BseMI GCAATG 1 cut(s) 25
BseMII CTCAG 3 cut(s) 286, 302, 347
BseNI ACTGG 1 cut(s) 368
BseXI GCAGC 3 cut(s) 27, 35, 38
BseYI CCCAGC 1 cut(s) 375
Bsh1285I CGRYCG 1 cut(s) 115
BshFI GGCC 5 cut(s) 5, 44, 96, 160, 188
BsiEI CGRYCG 1 cut(s) 115
BslFI GGGAC 2 cut(s) 95, 392
BslI CCNNNNNNNGG 3 cut(s) 78, 97, 142
BsmAI GTCTC 2 cut(s) 64, 275
BsmBI CGTCTC 1 cut(s) 64
BsmFI GGGAC 2 cut(s) 95, 392
BsnI GGCC 5 cut(s) 5, 44, 96, 160, 188
Bsp143I GATC 2 cut(s) 259, 306
Bsp19I CCATGG 1 cut(s) 6
BspACI CCGC 2 cut(s) 115, 166
BspANI GGCC 5 cut(s) 5, 44, 96, 160, 188
BspCNI CTCAG 3 cut(s) 285, 301, 346
BspPI GGATC 1 cut(s) 314
BsrDI GCAATG 1 cut(s) 25
BsrI ACTGG 1 cut(s) 368
BssECI CCNNGG 3 cut(s) 6, 75, 91
BssMI GATC 2 cut(s) 259, 306
BssT1I CCWWGG 2 cut(s) 6, 75
Bst2UI CCWGG 3 cut(s) 92, 98, 329
Bst4CI ACNGT 2 cut(s) 251, 388
BstDEI CTNAG 5 cut(s) 162, 272, 288, 333, 357
BstDSI CCRYGG 1 cut(s) 6
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 3 cut(s) 16, 126, 132
BstKTI GATC 2 cut(s) 262, 309
BstMAI GTCTC 2 cut(s) 64, 275
BstMBI GATC 2 cut(s) 259, 306
BstMCI CGRYCG 1 cut(s) 115
BstNI CCWGG 3 cut(s) 92, 98, 329
BstSCI CCNGG 3 cut(s) 90, 96, 327
BstV1I GCAGC 3 cut(s) 27, 35, 38
BstV2I GAAGAC 1 cut(s) 234
BstXI CCANNNNNNTGG 1 cut(s) 375
BsuRI GGCC 5 cut(s) 5, 44, 96, 160, 188
BtgI CCRYGG 1 cut(s) 6
BtsCI GGATG 3 cut(s) 16, 126, 132
Cfr13I GGNCC 2 cut(s) 94, 158
CseI GACGC 1 cut(s) 370
Csp6I GTAC 1 cut(s) 371
CviAII CATG 1 cut(s) 7
CviQI GTAC 1 cut(s) 371
DdeI CTNAG 5 cut(s) 162, 272, 288, 333, 357
DpnI GATC 2 cut(s) 261, 308
DpnII GATC 2 cut(s) 259, 306
DrdI GACNNNNNNGTC 1 cut(s) 386
DseDI GACNNNNNNGTC 1 cut(s) 386
EaeI YGGCCR 2 cut(s) 3, 42
Eco130I CCWWGG 2 cut(s) 6, 75
Eco57I CTGAAG 1 cut(s) 246
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 158
EcoRII CCWGG 3 cut(s) 90, 96, 327
EcoT14I CCWWGG 2 cut(s) 6, 75
ErhI CCWWGG 2 cut(s) 6, 75
Esp3I CGTCTC 1 cut(s) 64
FaeI CATG 1 cut(s) 10
FaiI YATR 4 cut(s) 8, 195, 392, 427
FalI AAGNNNNNCTT 4 cut(s) 59, 91, 219, 251
FaqI GGGAC 2 cut(s) 95, 392
FatI CATG 1 cut(s) 6
Fnu4HI GCNGC 3 cut(s) 16, 24, 27
FokI GGATG 3 cut(s) 23, 113, 119
Fsp4HI GCNGC 3 cut(s) 16, 24, 27
FspBI CTAG 1 cut(s) 436
GluI GCNGC 3 cut(s) 16, 24, 27
GsaI CCCAGC 1 cut(s) 379
HaeIII GGCC 5 cut(s) 5, 44, 96, 160, 188
HgaI GACGC 1 cut(s) 370
Hin1II CATG 1 cut(s) 10
HphI GGTGA 2 cut(s) 97, 313
Hpy188I TCNGA 3 cut(s) 275, 311, 336
Hpy188III TCNNGA 2 cut(s) 67, 140
HpyAV CCTTC 2 cut(s) 118, 146
HpyCH4III ACNGT 2 cut(s) 251, 388
HpyCH4V TGCA 1 cut(s) 23
HpyF3I CTNAG 5 cut(s) 162, 272, 288, 333, 357
Hsp92II CATG 1 cut(s) 10
Kzo9I GATC 2 cut(s) 259, 306
Lsp1109I GCAGC 3 cut(s) 27, 35, 38
LweI GCATC 1 cut(s) 337
MaeI CTAG 1 cut(s) 436
MaeIII GTNAC 2 cut(s) 251, 319
MalI GATC 2 cut(s) 261, 308
MboI GATC 2 cut(s) 259, 306
MboII GAAGA 2 cut(s) 239, 422
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 38
MluNI TGGCCA 1 cut(s) 5
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 7 cut(s) 28, 130, 136, 140, 149, 297, 400
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 341
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 243, 264
MslI CAYNNNNRTG 1 cut(s) 395
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 3 cut(s) 92, 98, 329
MvaI CCWGG 3 cut(s) 92, 98, 329
NcoI CCATGG 1 cut(s) 6
NdeII GATC 2 cut(s) 259, 306
NlaIII CATG 1 cut(s) 10
NmuCI GTSAC 1 cut(s) 319
PdmI GAANNNNTTC 1 cut(s) 341
PkrI GCNGC 3 cut(s) 17, 25, 28
Psp6I CCWGG 3 cut(s) 90, 96, 327
PspFI CCCAGC 1 cut(s) 375
PspGI CCWGG 3 cut(s) 90, 96, 327
PspPI GGNCC 2 cut(s) 94, 158
RsaI GTAC 1 cut(s) 372
RsaNI GTAC 1 cut(s) 371
RseI CAYNNNNRTG 1 cut(s) 395
SaqAI TTAA 2 cut(s) 243, 264
SatI GCNGC 3 cut(s) 16, 24, 27
Sau3AI GATC 2 cut(s) 259, 306
Sau96I GGNCC 2 cut(s) 94, 158
ScrFI CCNGG 3 cut(s) 92, 98, 329
SetI ASST 8 cut(s) 52, 77, 102, 110, 212, 226, 258, 411
SfaNI GCATC 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
Sse9I AATT 1 cut(s) 38
SsiI CCGC 2 cut(s) 115, 166
SspMI CTAG 1 cut(s) 436
StyD4I CCNGG 3 cut(s) 90, 96, 327
StyI CCWWGG 2 cut(s) 6, 75
TaaI ACNGT 2 cut(s) 251, 388
TaqI TCGA 2 cut(s) 111, 345
TasI AATT 1 cut(s) 38
Tru1I TTAA 2 cut(s) 243, 264
Tru9I TTAA 2 cut(s) 243, 264
TseFI GTSAC 1 cut(s) 319
TseI GCWGC 3 cut(s) 15, 23, 26
Tsp45I GTSAC 1 cut(s) 319
TspDTI ATGAA 1 cut(s) 416
TspGWI ACGGA 1 cut(s) 47
XagI CCTNNNNNAGG 1 cut(s) 140
XmnI GAANNNNTTC 1 cut(s) 341
XspI CTAG 1 cut(s) 436
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.