A domain family that is part of the cupin metalloenzyme superfamily.
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 4293 |
| AarI | CACCTGC | 1 cut(s) | 1898 |
| AasI | GACNNNNNNGTC | 3 cut(s) | 912, 2423, 3459 |
| Acc36I | ACCTGC | 1 cut(s) | 1898 |
| Acc65I | GGTACC | 2 cut(s) | 2631, 3260 |
| AccB1I | GGYRCC | 5 cut(s) | 785, 2538, 2631, 3260, 4316 |
| AccI | GTMKAC | 7 cut(s) | 775, 904, 1132, 1504, 1525, 1730, 2857 |
| AccII | CGCG | 1 cut(s) | 1128 |
| AciI | CCGC | 7 cut(s) | 89, 153, 165, 447, 1128, 1751, 4724 |
| AclI | AACGTT | 1 cut(s) | 4623 |
| AclWI | GGATC | 5 cut(s) | 129, 667, 4051, 4196, 4674 |
| AcoI | YGGCCR | 5 cut(s) | 1484, 1668, 1718, 3112, 3843 |
| AcsI | RAATTY | 8 cut(s) | 105, 348, 371, 2255, 2300, 3836, 4183, 4388 |
| AcuI | CTGAAG | 9 cut(s) | 711, 938, 2109, 3800, 4068, 4131, 4206, 4502, 4778 |
| AcvI | CACGTG | 1 cut(s) | 1861 |
| AcyI | GRCGYC | 1 cut(s) | 4317 |
| AflII | CTTAAG | 2 cut(s) | 2489, 2807 |
| AgeI | ACCGGT | 1 cut(s) | 1205 |
| AjiI | CACGTC | 1 cut(s) | 4180 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2747, 2779, 3914, 3946 |
| AleI | CACNNNNGTG | 2 cut(s) | 1863, 4527 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 1464, 1496, 2133, 2165 |
| Alw21I | GWGCWC | 3 cut(s) | 625, 2039, 2389 |
| AlwI | GGATC | 5 cut(s) | 129, 667, 4051, 4196, 4674 |
| AlwNI | CAGNNNCTG | 3 cut(s) | 2183, 2967, 3215 |
| Ama87I | CYCGRG | 1 cut(s) | 2636 |
| ApoI | RAATTY | 8 cut(s) | 105, 348, 371, 2255, 2300, 3836, 4183, 4388 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 2064, 2068, 2096, 2100, 4136, 4168 |
| AseI | ATTAAT | 1 cut(s) | 3705 |
| AsiGI | ACCGGT | 1 cut(s) | 1205 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 348, 1038, 4623, 4635, 4771 |
| Asp718I | GGTACC | 2 cut(s) | 2631, 3260 |
| AspLEI | GCGC | 4 cut(s) | 1128, 2161, 2476, 4319 |
| AspS9I | GGNCC | 9 cut(s) | 884, 1211, 2485, 2884, 3331, 3935, 4189, 4197, 4597 |
| AsuC2I | CCSGG | 1 cut(s) | 3485 |
| AvaI | CYCGRG | 1 cut(s) | 2636 |
| AvaII | GGWCC | 7 cut(s) | 1211, 2485, 2884, 3331, 3935, 4189, 4197 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1097, 1130 |
| BalI | TGGCCA | 3 cut(s) | 1670, 3114, 3845 |
| BanI | GGYRCC | 5 cut(s) | 785, 2538, 2631, 3260, 4316 |
| BanII | GRGCYC | 1 cut(s) | 2039 |
| BauI | CACGAG | 1 cut(s) | 2890 |
| BbrPI | CACGTG | 1 cut(s) | 1861 |
| BbsI | GAAGAC | 1 cut(s) | 2648 |
| Bbv12I | GWGCWC | 3 cut(s) | 625, 2039, 2389 |
| BbvCI | CCTCAGC | 1 cut(s) | 984 |
| BceAI | ACGGC | 2 cut(s) | 4304, 4803 |
| BcnI | CCSGG | 1 cut(s) | 3485 |
| BfaI | CTAG | 9 cut(s) | 770, 2051, 2208, 2331, 2439, 2903, 3198, 4457, 4804 |
| BfmI | CTRYAG | 5 cut(s) | 1109, 1887, 2154, 2175, 2468 |
| BfoI | RGCGCY | 1 cut(s) | 4320 |
| BfrI | CTTAAG | 2 cut(s) | 2489, 2807 |
| BfuAI | ACCTGC | 1 cut(s) | 1898 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1667 |
| BglII | AGATCT | 2 cut(s) | 2831, 4800 |
| BmcAI | AGTACT | 2 cut(s) | 2112, 3642 |
| Bme18I | GGWCC | 7 cut(s) | 1211, 2485, 2884, 3331, 3935, 4189, 4197 |
| BmeT110I | CYCGRG | 1 cut(s) | 2636 |
| BmgBI | CACGTC | 1 cut(s) | 4180 |
| BmgT120I | GGNCC | 9 cut(s) | 884, 1211, 2485, 2884, 3331, 3935, 4189, 4197, 4597 |
| BmrI | ACTGGG | 4 cut(s) | 309, 3257, 3259, 3310 |
| BmuI | ACTGGG | 4 cut(s) | 309, 3257, 3259, 3310 |
| BoxI | GACNNNNGTC | 1 cut(s) | 3933 |
| BpiI | GAAGAC | 1 cut(s) | 2648 |
| BpmI | CTGGAG | 3 cut(s) | 3556, 4047, 4718 |
| Bpu10I | CCTNAGC | 2 cut(s) | 984, 3590 |
| BpuEI | CTTGAG | 3 cut(s) | 609, 2693, 3357 |
| BpuMI | CCSGG | 1 cut(s) | 3485 |
| BsaAI | YACGTR | 2 cut(s) | 1861, 2861 |
| BsaBI | GATNNNNATC | 1 cut(s) | 2913 |
| BsaHI | GRCGYC | 1 cut(s) | 4317 |
| BsaI | GGTCTC | 5 cut(s) | 1954, 2209, 2332, 2428, 2440 |
| BsaWI | WCCGGW | 2 cut(s) | 1205, 1249 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 1992, 2022, 3975, 4005, 4305, 4335 |
| Bse118I | RCCGGY | 2 cut(s) | 1205, 2055 |
| Bse3DI | GCAATG | 2 cut(s) | 1179, 3418 |
| Bse8I | GATNNNNATC | 1 cut(s) | 2913 |
| BseJI | GATNNNNATC | 1 cut(s) | 2913 |
| BseMI | GCAATG | 2 cut(s) | 1179, 3418 |
| BseRI | GAGGAG | 6 cut(s) | 155, 3553, 3665, 3983, 3996, 4664 |
| BseYI | CCCAGC | 2 cut(s) | 2732, 3594 |
| BsgI | GTGCAG | 1 cut(s) | 1557 |
| Bsh1236I | CGCG | 1 cut(s) | 1128 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1132 |
| BshNI | GGYRCC | 5 cut(s) | 785, 2538, 2631, 3260, 4316 |
| BshTI | ACCGGT | 1 cut(s) | 1205 |
| BsiEI | CGRYCG | 1 cut(s) | 1132 |
| BsiHKAI | GWGCWC | 3 cut(s) | 625, 2039, 2389 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2636 |
| BsiSI | CCGG | 4 cut(s) | 1206, 1250, 2056, 3485 |
| BslFI | GGGAC | 4 cut(s) | 310, 2498, 4210, 4340 |
| BsmBI | CGTCTC | 2 cut(s) | 2065, 4778 |
| BsmFI | GGGAC | 4 cut(s) | 310, 2498, 4210, 4340 |
| BsmI | GAATGC | 5 cut(s) | 417, 462, 1653, 4635, 4637 |
| Bso31I | GGTCTC | 5 cut(s) | 1954, 2209, 2332, 2428, 2440 |
| BsoBI | CYCGRG | 1 cut(s) | 2636 |
| Bsp1286I | GDGCHC | 3 cut(s) | 625, 2039, 2389 |
| Bsp1407I | TGTACA | 2 cut(s) | 2866, 3493 |
| BspACI | CCGC | 7 cut(s) | 89, 153, 165, 447, 1128, 1751, 4724 |
| BspFNI | CGCG | 1 cut(s) | 1128 |
| BspHI | TCATGA | 2 cut(s) | 3463, 4438 |
| BspMAI | CTGCAG | 3 cut(s) | 1113, 1891, 2179 |
| BspMI | ACCTGC | 1 cut(s) | 1898 |
| BspPI | GGATC | 5 cut(s) | 129, 667, 4051, 4196, 4674 |
| BspT107I | GGYRCC | 5 cut(s) | 785, 2538, 2631, 3260, 4316 |
| BspTI | CTTAAG | 2 cut(s) | 2489, 2807 |
| BspTNI | GGTCTC | 5 cut(s) | 1954, 2209, 2332, 2428, 2440 |
| BsrDI | GCAATG | 2 cut(s) | 1179, 3418 |
| BsrFI | RCCGGY | 2 cut(s) | 1205, 2055 |
| BsrGI | TGTACA | 2 cut(s) | 2866, 3493 |
| BssAI | RCCGGY | 2 cut(s) | 1205, 2055 |
| BssNAI | GTATAC | 1 cut(s) | 2858 |
| BssNI | GRCGYC | 1 cut(s) | 4317 |
| BssSI | CACGAG | 1 cut(s) | 2890 |
| BssT1I | CCWWGG | 5 cut(s) | 442, 3821, 4192, 4505, 4525 |
| Bst1107I | GTATAC | 1 cut(s) | 2858 |
| Bst2BI | CACGAG | 1 cut(s) | 2890 |
| Bst6I | CTCTTC | 8 cut(s) | 687, 738, 755, 2063, 2195, 2874, 3804, 4482 |
| BstACI | GRCGYC | 1 cut(s) | 4317 |
| BstAFI | CTTAAG | 2 cut(s) | 2489, 2807 |
| BstAPI | GCANNNNNTGC | 4 cut(s) | 1200, 2703, 4721, 4733 |
| BstAUI | TGTACA | 2 cut(s) | 2866, 3493 |
| BstBAI | YACGTR | 2 cut(s) | 1861, 2861 |
| BstC8I | GCNNGC | 5 cut(s) | 1013, 2057, 3409, 3441, 4661 |
| BstENI | CCTNNNNNAGG | 3 cut(s) | 2492, 2711, 4529 |
| BstFNI | CGCG | 1 cut(s) | 1128 |
| BstH2I | RGCGCY | 1 cut(s) | 4320 |
| BstHHI | GCGC | 4 cut(s) | 1128, 2161, 2476, 4319 |
| BstMCI | CGRYCG | 1 cut(s) | 1132 |
| BstNSI | RCATGY | 3 cut(s) | 3390, 3411, 3443 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 3933 |
| BstSFI | CTRYAG | 5 cut(s) | 1109, 1887, 2154, 2175, 2468 |
| BstUI | CGCG | 1 cut(s) | 1128 |
| BstV2I | GAAGAC | 1 cut(s) | 2648 |
| BstX2I | RGATCY | 5 cut(s) | 121, 2831, 4056, 4679, 4800 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1019 |
| BstYI | RGATCY | 5 cut(s) | 121, 2831, 4056, 4679, 4800 |
| BstZ17I | GTATAC | 1 cut(s) | 2858 |
| BtgZI | GCGATG | 1 cut(s) | 3934 |
| BtrI | CACGTC | 1 cut(s) | 4180 |
| BtsI | GCAGTG | 2 cut(s) | 867, 3052 |
| BtsIMutI | CAGTG | 9 cut(s) | 867, 2257, 2380, 2552, 3052, 3245, 3336, 3532, 4715 |
| BveI | ACCTGC | 1 cut(s) | 1898 |
| Cac8I | GCNNGC | 5 cut(s) | 1013, 2057, 3409, 3441, 4661 |
| CaiI | CAGNNNCTG | 3 cut(s) | 2183, 2967, 3215 |
| CciI | TCATGA | 2 cut(s) | 3463, 4438 |
| CfoI | GCGC | 4 cut(s) | 1128, 2161, 2476, 4319 |
| Cfr10I | RCCGGY | 2 cut(s) | 1205, 2055 |
| Cfr13I | GGNCC | 9 cut(s) | 884, 1211, 2485, 2884, 3331, 3935, 4189, 4197, 4597 |
| CspAI | ACCGGT | 1 cut(s) | 1205 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 718, 753 |
| DinI | GGCGCC | 1 cut(s) | 4318 |
| DraI | TTTAAA | 1 cut(s) | 2586 |
| DrdI | GACNNNNNNGTC | 3 cut(s) | 912, 2423, 3459 |
| DseDI | GACNNNNNNGTC | 3 cut(s) | 912, 2423, 3459 |
| EaeI | YGGCCR | 5 cut(s) | 1484, 1668, 1718, 3112, 3843 |
| Eam1104I | CTCTTC | 8 cut(s) | 687, 738, 755, 2063, 2195, 2874, 3804, 4482 |
| EarI | CTCTTC | 8 cut(s) | 687, 738, 755, 2063, 2195, 2874, 3804, 4482 |
| Ecl136II | GAGCTC | 1 cut(s) | 2037 |
| Eco130I | CCWWGG | 5 cut(s) | 442, 3821, 4192, 4505, 4525 |
| Eco147I | AGGCCT | 6 cut(s) | 441, 465, 503, 1852, 2206, 2329 |
| Eco24I | GRGCYC | 1 cut(s) | 2039 |
| Eco31I | GGTCTC | 5 cut(s) | 1954, 2209, 2332, 2428, 2440 |
| Eco47I | GGWCC | 7 cut(s) | 1211, 2485, 2884, 3331, 3935, 4189, 4197 |
| Eco53kI | GAGCTC | 1 cut(s) | 2037 |
| Eco57I | CTGAAG | 9 cut(s) | 711, 938, 2109, 3800, 4068, 4131, 4206, 4502, 4778 |
| Eco72I | CACGTG | 1 cut(s) | 1861 |
| Eco88I | CYCGRG | 1 cut(s) | 2636 |
| EcoICRI | GAGCTC | 1 cut(s) | 2037 |
| EcoNI | CCTNNNNNAGG | 3 cut(s) | 2492, 2711, 4529 |
| EcoO109I | RGGNCCY | 4 cut(s) | 2485, 2884, 3331, 3935 |
| EcoRI | GAATTC | 1 cut(s) | 2255 |
| EcoT14I | CCWWGG | 5 cut(s) | 442, 3821, 4192, 4505, 4525 |
| EcoT22I | ATGCAT | 4 cut(s) | 556, 1478, 2121, 4637 |
| EcoT38I | GRGCYC | 1 cut(s) | 2039 |
| EgeI | GGCGCC | 1 cut(s) | 4318 |
| EheI | GGCGCC | 1 cut(s) | 4318 |
| ErhI | CCWWGG | 5 cut(s) | 442, 3821, 4192, 4505, 4525 |
| Esp3I | CGTCTC | 2 cut(s) | 2065, 4778 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1023, 1055, 3914, 3946 |
| FaqI | GGGAC | 4 cut(s) | 310, 2498, 4210, 4340 |
| FauI | CCCGC | 1 cut(s) | 82 |
| FauNDI | CATATG | 1 cut(s) | 4309 |
| FblI | GTMKAC | 7 cut(s) | 775, 904, 1132, 1504, 1525, 1730, 2857 |
| FriOI | GRGCYC | 1 cut(s) | 2039 |
| FseI | GGCCGGCC | 1 cut(s) | 2059 |
| FspBI | CTAG | 9 cut(s) | 770, 2051, 2208, 2331, 2439, 2903, 3198, 4457, 4804 |
| GlaI | GCGC | 4 cut(s) | 1127, 2160, 2475, 4318 |
| GsaI | CCCAGC | 2 cut(s) | 2736, 3598 |
| GsuI | CTGGAG | 3 cut(s) | 3556, 4047, 4718 |
| HaeII | RGCGCY | 1 cut(s) | 4320 |
| HapII | CCGG | 4 cut(s) | 1206, 1250, 2056, 3485 |
| HhaI | GCGC | 4 cut(s) | 1128, 2161, 2476, 4319 |
| Hin1I | GRCGYC | 1 cut(s) | 4317 |
| Hin6I | GCGC | 4 cut(s) | 1126, 2159, 2474, 4317 |
| HinP1I | GCGC | 4 cut(s) | 1126, 2159, 2474, 4317 |
| HindIII | AAGCTT | 4 cut(s) | 200, 1449, 2091, 3423 |
| HpaI | GTTAAC | 3 cut(s) | 1303, 4359, 4644 |
| HpaII | CCGG | 4 cut(s) | 1206, 1250, 2056, 3485 |
| Hpy99I | CGWCG | 1 cut(s) | 4341 |
| HpyCH4IV | ACGT | 7 cut(s) | 1860, 2860, 3283, 4179, 4302, 4511, 4623 |
| HpySE526I | ACGT | 7 cut(s) | 1860, 2860, 3283, 4179, 4302, 4511, 4623 |
| Hsp92I | GRCGYC | 1 cut(s) | 4317 |
| HspAI | GCGC | 4 cut(s) | 1126, 2159, 2474, 4317 |
| KasI | GGCGCC | 1 cut(s) | 4316 |
| KpnI | GGTACC | 2 cut(s) | 2635, 3264 |
| KroI | GCCGGC | 1 cut(s) | 2055 |
| KroNI | GCCGGC | 1 cut(s) | 2057 |
| KspAI | GTTAAC | 3 cut(s) | 1303, 4359, 4644 |
| LmnI | GCTCC | 4 cut(s) | 686, 1828, 2824, 3970 |
| MaeI | CTAG | 9 cut(s) | 770, 2051, 2208, 2331, 2439, 2903, 3198, 4457, 4804 |
| MaeII | ACGT | 7 cut(s) | 1860, 2860, 3283, 4179, 4302, 4511, 4623 |
| MfeI | CAATTG | 4 cut(s) | 2393, 2692, 2870, 3527 |
| MflI | RGATCY | 5 cut(s) | 121, 2831, 4056, 4679, 4800 |
| MhlI | GDGCHC | 3 cut(s) | 625, 2039, 2389 |
| MlsI | TGGCCA | 3 cut(s) | 1670, 3114, 3845 |
| MluNI | TGGCCA | 3 cut(s) | 1670, 3114, 3845 |
| Mly113I | GGCGCC | 1 cut(s) | 4317 |
| MlyI | GAGTC | 4 cut(s) | 1085, 1240, 1999, 4030 |
| MmeI | TCCRAC | 5 cut(s) | 1075, 1660, 1951, 1957, 3182 |
| Mox20I | TGGCCA | 3 cut(s) | 1670, 3114, 3845 |
| Mph1103I | ATGCAT | 4 cut(s) | 556, 1478, 2121, 4637 |
| MroNI | GCCGGC | 1 cut(s) | 2055 |
| MroXI | GAANNNNTTC | 5 cut(s) | 348, 1038, 4623, 4635, 4771 |
| MscI | TGGCCA | 3 cut(s) | 1670, 3114, 3845 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1416, 1863, 4527, 4592 |
| Msp20I | TGGCCA | 3 cut(s) | 1670, 3114, 3845 |
| MspA1I | CMGCKG | 2 cut(s) | 56, 3446 |
| MspCI | CTTAAG | 2 cut(s) | 2489, 2807 |
| MspI | CCGG | 4 cut(s) | 1206, 1250, 2056, 3485 |
| MunI | CAATTG | 4 cut(s) | 2393, 2692, 2870, 3527 |
| Mva1269I | GAATGC | 5 cut(s) | 417, 462, 1653, 4635, 4637 |
| MvnI | CGCG | 1 cut(s) | 1128 |
| NaeI | GCCGGC | 1 cut(s) | 2057 |
| NarI | GGCGCC | 1 cut(s) | 4317 |
| NciI | CCSGG | 1 cut(s) | 3485 |
| NdeI | CATATG | 1 cut(s) | 4309 |
| NgoMIV | GCCGGC | 1 cut(s) | 2055 |
| NmeAIII | GCCGAG | 1 cut(s) | 2368 |
| NmuCI | GTSAC | 7 cut(s) | 98, 236, 2375, 2927, 3001, 3860, 4007 |
| NsiI | ATGCAT | 4 cut(s) | 556, 1478, 2121, 4637 |
| NspI | RCATGY | 3 cut(s) | 3390, 3411, 3443 |
| OliI | CACNNNNGTG | 2 cut(s) | 1863, 4527 |
| PaeI | GCATGC | 2 cut(s) | 3411, 3443 |
| PagI | TCATGA | 2 cut(s) | 3463, 4438 |
| PaqCI | CACCTGC | 1 cut(s) | 1898 |
| PasI | CCCWGGG | 1 cut(s) | 2481 |
| PceI | AGGCCT | 6 cut(s) | 441, 465, 503, 1852, 2206, 2329 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 2080 |
| PctI | GAATGC | 5 cut(s) | 417, 462, 1653, 4635, 4637 |
| PdiI | GCCGGC | 1 cut(s) | 2057 |
| PdmI | GAANNNNTTC | 5 cut(s) | 348, 1038, 4623, 4635, 4771 |
| PfeI | GAWTC | 8 cut(s) | 2076, 2245, 2368, 3056, 3488, 3986, 4265, 4767 |
| PfoI | TCCNGGA | 1 cut(s) | 1150 |
| PinAI | ACCGGT | 1 cut(s) | 1205 |
| PleI | GAGTC | 4 cut(s) | 1084, 1240, 1998, 4030 |
| PluTI | GGCGCC | 1 cut(s) | 4320 |
| PmaCI | CACGTG | 1 cut(s) | 1861 |
| PmlI | CACGTG | 1 cut(s) | 1861 |
| PpsI | GAGTC | 4 cut(s) | 1084, 1240, 1998, 4030 |
| Ppu21I | YACGTR | 2 cut(s) | 1861, 2861 |
| PpuMI | RGGWCCY | 4 cut(s) | 2485, 2884, 3331, 3935 |
| PshAI | GACNNNNGTC | 1 cut(s) | 3933 |
| PshBI | ATTAAT | 1 cut(s) | 3705 |
| PsiI | TTATAA | 1 cut(s) | 4293 |
| Psp124BI | GAGCTC | 1 cut(s) | 2039 |
| Psp1406I | AACGTT | 1 cut(s) | 4623 |
| Psp5II | RGGWCCY | 4 cut(s) | 2485, 2884, 3331, 3935 |
| PspCI | CACGTG | 1 cut(s) | 1861 |
| PspFI | CCCAGC | 2 cut(s) | 2732, 3594 |
| PspPI | GGNCC | 9 cut(s) | 884, 1211, 2485, 2884, 3331, 3935, 4189, 4197, 4597 |
| PspPPI | RGGWCCY | 4 cut(s) | 2485, 2884, 3331, 3935 |
| PstI | CTGCAG | 3 cut(s) | 1113, 1891, 2179 |
| PstNI | CAGNNNCTG | 3 cut(s) | 2183, 2967, 3215 |
| PsuI | RGATCY | 5 cut(s) | 121, 2831, 4056, 4679, 4800 |
| PvuII | CAGCTG | 2 cut(s) | 56, 3446 |
| RigI | GGCCGGCC | 1 cut(s) | 2059 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1416, 1863, 4527, 4592 |
| SacI | GAGCTC | 1 cut(s) | 2039 |
| SalI | GTCGAC | 6 cut(s) | 774, 903, 1131, 1503, 1524, 1729 |
| Sau96I | GGNCC | 9 cut(s) | 884, 1211, 2485, 2884, 3331, 3935, 4189, 4197, 4597 |
| ScaI | AGTACT | 2 cut(s) | 2112, 3642 |
| SchI | GAGTC | 4 cut(s) | 1085, 1240, 1999, 4030 |
| SduI | GDGCHC | 3 cut(s) | 625, 2039, 2389 |
| SfcI | CTRYAG | 5 cut(s) | 1109, 1887, 2154, 2175, 2468 |
| SfoI | GGCGCC | 1 cut(s) | 4318 |
| SgrAI | CRCCGGYG | 1 cut(s) | 1205 |
| SinI | GGWCC | 7 cut(s) | 1211, 2485, 2884, 3331, 3935, 4189, 4197 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1416, 1863, 4527, 4592 |
| SmlI | CTYRAG | 5 cut(s) | 624, 2489, 2708, 2807, 3372 |
| SmoI | CTYRAG | 5 cut(s) | 624, 2489, 2708, 2807, 3372 |
| SphI | GCATGC | 2 cut(s) | 3411, 3443 |
| SseBI | AGGCCT | 6 cut(s) | 441, 465, 503, 1852, 2206, 2329 |
| SsiI | CCGC | 7 cut(s) | 89, 153, 165, 447, 1128, 1751, 4724 |
| SspDI | GGCGCC | 1 cut(s) | 4316 |
| SspI | AATATT | 3 cut(s) | 682, 2758, 3709 |
| SspMI | CTAG | 9 cut(s) | 770, 2051, 2208, 2331, 2439, 2903, 3198, 4457, 4804 |
| SstI | GAGCTC | 1 cut(s) | 2039 |
| StuI | AGGCCT | 6 cut(s) | 441, 465, 503, 1852, 2206, 2329 |
| StyI | CCWWGG | 5 cut(s) | 442, 3821, 4192, 4505, 4525 |
| TaiI | ACGT | 7 cut(s) | 1863, 2863, 3286, 4182, 4305, 4514, 4626 |
| TaqII | GACCGA | 1 cut(s) | 4214 |
| TatI | WGTACW | 7 cut(s) | 2110, 2866, 2922, 3085, 3493, 3568, 3640 |
| TauI | GCSGC | 1 cut(s) | 4727 |
| TfiI | GAWTC | 8 cut(s) | 2076, 2245, 2368, 3056, 3488, 3986, 4265, 4767 |
| TscAI | CASTG | 9 cut(s) | 867, 2257, 2380, 2552, 3052, 3252, 3343, 3539, 4722 |
| TseFI | GTSAC | 7 cut(s) | 98, 236, 2375, 2927, 3001, 3860, 4007 |
| Tsp45I | GTSAC | 7 cut(s) | 98, 236, 2375, 2927, 3001, 3860, 4007 |
| TspGWI | ACGGA | 5 cut(s) | 1331, 2249, 2372, 2569, 4325 |
| TspRI | CASTG | 9 cut(s) | 867, 2257, 2380, 2552, 3052, 3252, 3343, 3539, 4722 |
| Vha464I | CTTAAG | 2 cut(s) | 2489, 2807 |
| VpaK11BI | GGWCC | 7 cut(s) | 1211, 2485, 2884, 3331, 3935, 4189, 4197 |
| VspI | ATTAAT | 1 cut(s) | 3705 |
| XagI | CCTNNNNNAGG | 3 cut(s) | 2492, 2711, 4529 |
| XapI | RAATTY | 8 cut(s) | 105, 348, 371, 2255, 2300, 3836, 4183, 4388 |
| XbaI | TCTAGA | 1 cut(s) | 4456 |
| XceI | RCATGY | 3 cut(s) | 3390, 3411, 3443 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 3609 |
| XmiI | GTMKAC | 7 cut(s) | 775, 904, 1132, 1504, 1525, 1730, 2857 |
| XmnI | GAANNNNTTC | 5 cut(s) | 348, 1038, 4623, 4635, 4771 |
| XspI | CTAG | 9 cut(s) | 770, 2051, 2208, 2331, 2439, 2903, 3198, 4457, 4804 |
| ZrmI | AGTACT | 2 cut(s) | 2112, 3642 |
| Zsp2I | ATGCAT | 4 cut(s) | 556, 1478, 2121, 4637 |