The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Coatomer_E | PF04733 | 2 - 61 | 4.6e-14 | Coatomer epsilon subunit |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 161 |
| AclWI | GGATC | 2 cut(s) | 44, 57 |
| AgsI | TTSAA | 1 cut(s) | 26 |
| AjiI | CACGTC | 1 cut(s) | 148 |
| AjnI | CCWGG | 1 cut(s) | 69 |
| AluBI | AGCT | 2 cut(s) | 170, 184 |
| AluI | AGCT | 2 cut(s) | 170, 184 |
| AlwI | GGATC | 2 cut(s) | 44, 57 |
| AsuHPI | GGTGA | 1 cut(s) | 80 |
| BamHI | GGATCC | 1 cut(s) | 49 |
| BanI | GGYRCC | 1 cut(s) | 161 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 69, 101 |
| BbsI | GAAGAC | 1 cut(s) | 77 |
| BbvCI | CCTCAGC | 1 cut(s) | 166 |
| BciT130I | CCWGG | 1 cut(s) | 71 |
| BfaI | CTAG | 1 cut(s) | 62 |
| BfmI | CTRYAG | 1 cut(s) | 171 |
| Bme1390I | CCNGG | 1 cut(s) | 71 |
| BmgBI | CACGTC | 1 cut(s) | 148 |
| BmiI | GGNNCC | 2 cut(s) | 51, 163 |
| BmrFI | CCNGG | 1 cut(s) | 71 |
| BmsI | GCATC | 1 cut(s) | 31 |
| BpiI | GAAGAC | 1 cut(s) | 77 |
| Bpu10I | CCTNAGC | 1 cut(s) | 166 |
| BsaJI | CCNNGG | 2 cut(s) | 29, 91 |
| BseBI | CCWGG | 1 cut(s) | 71 |
| BseDI | CCNNGG | 2 cut(s) | 29, 91 |
| BseGI | GGATG | 2 cut(s) | 46, 136 |
| BseMII | CTCAG | 1 cut(s) | 180 |
| BshNI | GGYRCC | 1 cut(s) | 161 |
| Bsp143I | GATC | 1 cut(s) | 49 |
| Bsp19I | CCATGG | 1 cut(s) | 29 |
| BspCNI | CTCAG | 1 cut(s) | 179 |
| BspLI | GGNNCC | 2 cut(s) | 51, 163 |
| BspPI | GGATC | 2 cut(s) | 44, 57 |
| BspT107I | GGYRCC | 1 cut(s) | 161 |
| BssECI | CCNNGG | 2 cut(s) | 29, 91 |
| BssMI | GATC | 1 cut(s) | 49 |
| BssT1I | CCWWGG | 2 cut(s) | 29, 91 |
| Bst2UI | CCWGG | 1 cut(s) | 71 |
| BstDEI | CTNAG | 1 cut(s) | 166 |
| BstDSI | CCRYGG | 1 cut(s) | 29 |
| BstF5I | GGATG | 2 cut(s) | 46, 136 |
| BstKTI | GATC | 1 cut(s) | 52 |
| BstMBI | GATC | 1 cut(s) | 49 |
| BstNI | CCWGG | 1 cut(s) | 71 |
| BstSCI | CCNGG | 1 cut(s) | 69 |
| BstSFI | CTRYAG | 1 cut(s) | 171 |
| BstV2I | GAAGAC | 1 cut(s) | 77 |
| BstX2I | RGATCY | 1 cut(s) | 49 |
| BstYI | RGATCY | 1 cut(s) | 49 |
| BtgI | CCRYGG | 1 cut(s) | 29 |
| BtrI | CACGTC | 1 cut(s) | 148 |
| BtsCI | GGATG | 2 cut(s) | 46, 136 |
| BtsI | GCAGTG | 1 cut(s) | 14 |
| BtsIMutI | CAGTG | 1 cut(s) | 14 |
| CsiI | ACCWGGT | 1 cut(s) | 69 |
| CviAII | CATG | 1 cut(s) | 30 |
| CviJI | RGCY | 4 cut(s) | 65, 120, 170, 184 |
| CviKI_1 | RGCY | 4 cut(s) | 65, 120, 170, 184 |
| DdeI | CTNAG | 1 cut(s) | 166 |
| DpnI | GATC | 1 cut(s) | 51 |
| DpnII | GATC | 1 cut(s) | 49 |
| Eco130I | CCWWGG | 2 cut(s) | 29, 91 |
| EcoRII | CCWGG | 1 cut(s) | 69 |
| EcoT14I | CCWWGG | 2 cut(s) | 29, 91 |
| ErhI | CCWWGG | 2 cut(s) | 29, 91 |
| FaeI | CATG | 1 cut(s) | 33 |
| FaiI | YATR | 2 cut(s) | 31, 79 |
| FatI | CATG | 1 cut(s) | 29 |
| FokI | GGATG | 2 cut(s) | 53, 123 |
| FspBI | CTAG | 1 cut(s) | 62 |
| Hin1II | CATG | 1 cut(s) | 33 |
| HphI | GGTGA | 1 cut(s) | 80 |
| Hpy188III | TCNNGA | 4 cut(s) | 23, 53, 126, 140 |
| HpyAV | CCTTC | 1 cut(s) | 114 |
| HpyCH4IV | ACGT | 1 cut(s) | 147 |
| HpyCH4V | TGCA | 2 cut(s) | 44, 134 |
| HpyF3I | CTNAG | 1 cut(s) | 166 |
| HpySE526I | ACGT | 1 cut(s) | 147 |
| Hsp92II | CATG | 1 cut(s) | 33 |
| Kzo9I | GATC | 1 cut(s) | 49 |
| LpnPI | CCDG | 4 cut(s) | 56, 66, 83, 153 |
| LweI | GCATC | 1 cut(s) | 31 |
| MabI | ACCWGGT | 1 cut(s) | 69 |
| MaeI | CTAG | 1 cut(s) | 62 |
| MaeII | ACGT | 1 cut(s) | 147 |
| MalI | GATC | 1 cut(s) | 51 |
| MboI | GATC | 1 cut(s) | 49 |
| MboII | GAAGA | 1 cut(s) | 77 |
| MflI | RGATCY | 1 cut(s) | 49 |
| MnlI | CCTC | 3 cut(s) | 40, 161, 175 |
| MspA1I | CMGCKG | 1 cut(s) | 170 |
| MspR9I | CCNGG | 1 cut(s) | 71 |
| MvaI | CCWGG | 1 cut(s) | 71 |
| NcoI | CCATGG | 1 cut(s) | 29 |
| NdeII | GATC | 1 cut(s) | 49 |
| NlaIII | CATG | 1 cut(s) | 33 |
| NlaIV | GGNNCC | 2 cut(s) | 51, 163 |
| PflFI | GACNNNGTC | 1 cut(s) | 146 |
| Psp6I | CCWGG | 1 cut(s) | 69 |
| PspGI | CCWGG | 1 cut(s) | 69 |
| PspN4I | GGNNCC | 2 cut(s) | 51, 163 |
| PsuI | RGATCY | 1 cut(s) | 49 |
| PsyI | GACNNNGTC | 1 cut(s) | 146 |
| PvuII | CAGCTG | 1 cut(s) | 170 |
| Sau3AI | GATC | 1 cut(s) | 49 |
| ScrFI | CCNGG | 1 cut(s) | 71 |
| SetI | ASST | 7 cut(s) | 72, 93, 106, 150, 163, 172, 186 |
| SexAI | ACCWGGT | 1 cut(s) | 69 |
| SfaNI | GCATC | 1 cut(s) | 31 |
| SfcI | CTRYAG | 1 cut(s) | 171 |
| SspMI | CTAG | 1 cut(s) | 62 |
| StyD4I | CCNGG | 1 cut(s) | 69 |
| StyI | CCWWGG | 2 cut(s) | 29, 91 |
| TaiI | ACGT | 1 cut(s) | 150 |
| TscAI | CASTG | 1 cut(s) | 21 |
| TspRI | CASTG | 1 cut(s) | 21 |
| Tth111I | GACNNNGTC | 1 cut(s) | 146 |
| XspI | CTAG | 1 cut(s) | 62 |