The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non- clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 31 |
| AciI | CCGC | 2 cut(s) | 12, 55 |
| AclWI | GGATC | 1 cut(s) | 214 |
| AcuI | CTGAAG | 2 cut(s) | 165, 228 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 229 |
| AluBI | AGCT | 3 cut(s) | 52, 110, 179 |
| AluI | AGCT | 3 cut(s) | 52, 110, 179 |
| Alw26I | GTCTC | 1 cut(s) | 89 |
| AlwI | GGATC | 1 cut(s) | 214 |
| ApeKI | GCWGC | 2 cut(s) | 49, 193 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 82, 114 |
| AxyI | CCTNAGG | 1 cut(s) | 164 |
| BbvI | GCAGC | 2 cut(s) | 61, 205 |
| BciVI | GTATCC | 1 cut(s) | 12 |
| BcoDI | GTCTC | 1 cut(s) | 89 |
| BfaI | CTAG | 2 cut(s) | 39, 235 |
| BfuAI | ACCTGC | 1 cut(s) | 31 |
| BfuI | GTATCC | 1 cut(s) | 12 |
| BisI | GCNGC | 3 cut(s) | 13, 50, 194 |
| BlsI | GCNGC | 3 cut(s) | 14, 51, 195 |
| BmsI | GCATC | 1 cut(s) | 35 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 36, 66 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 229 |
| Bse21I | CCTNAGG | 1 cut(s) | 164 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 229 |
| BseXI | GCAGC | 2 cut(s) | 61, 205 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 229 |
| BsmAI | GTCTC | 1 cut(s) | 89 |
| Bsp143I | GATC | 1 cut(s) | 219 |
| BspACI | CCGC | 2 cut(s) | 12, 55 |
| BspMI | ACCTGC | 1 cut(s) | 31 |
| BspPI | GGATC | 1 cut(s) | 214 |
| BssMI | GATC | 1 cut(s) | 219 |
| BstDEI | CTNAG | 2 cut(s) | 30, 164 |
| BstKTI | GATC | 1 cut(s) | 222 |
| BstMAI | GTCTC | 1 cut(s) | 89 |
| BstMBI | GATC | 1 cut(s) | 219 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 49 |
| BstV1I | GCAGC | 2 cut(s) | 61, 205 |
| BstX2I | RGATCY | 1 cut(s) | 219 |
| BstYI | RGATCY | 1 cut(s) | 219 |
| Bsu36I | CCTNAGG | 1 cut(s) | 164 |
| BsuI | GTATCC | 1 cut(s) | 12 |
| BveI | ACCTGC | 1 cut(s) | 31 |
| CviJI | RGCY | 4 cut(s) | 15, 52, 110, 179 |
| CviKI_1 | RGCY | 4 cut(s) | 15, 52, 110, 179 |
| DdeI | CTNAG | 2 cut(s) | 30, 164 |
| DpnI | GATC | 1 cut(s) | 221 |
| DpnII | GATC | 1 cut(s) | 219 |
| Eco57I | CTGAAG | 2 cut(s) | 165, 228 |
| Eco81I | CCTNAGG | 1 cut(s) | 164 |
| FaiI | YATR | 4 cut(s) | 76, 143, 171, 216 |
| Fnu4HI | GCNGC | 3 cut(s) | 13, 50, 194 |
| Fsp4HI | GCNGC | 3 cut(s) | 13, 50, 194 |
| FspBI | CTAG | 2 cut(s) | 39, 235 |
| GluI | GCNGC | 3 cut(s) | 13, 50, 194 |
| HinfI | GANTC | 1 cut(s) | 35 |
| Hpy188I | TCNGA | 2 cut(s) | 184, 208 |
| Hpy188III | TCNNGA | 1 cut(s) | 223 |
| HpyCH4V | TGCA | 1 cut(s) | 26 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 49 |
| HpyF3I | CTNAG | 2 cut(s) | 30, 164 |
| Kzo9I | GATC | 1 cut(s) | 219 |
| LmnI | GCTCC | 2 cut(s) | 57, 190 |
| LpnPI | CCDG | 2 cut(s) | 36, 96 |
| Lsp1109I | GCAGC | 2 cut(s) | 61, 205 |
| LweI | GCATC | 1 cut(s) | 35 |
| MaeI | CTAG | 2 cut(s) | 39, 235 |
| MalI | GATC | 1 cut(s) | 221 |
| MboI | GATC | 1 cut(s) | 219 |
| MflI | RGATCY | 1 cut(s) | 219 |
| MnlI | CCTC | 1 cut(s) | 164 |
| MseI | TTAA | 2 cut(s) | 131, 201 |
| MspA1I | CMGCKG | 1 cut(s) | 110 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 49 |
| NdeII | GATC | 1 cut(s) | 219 |
| PfeI | GAWTC | 1 cut(s) | 35 |
| PkrI | GCNGC | 3 cut(s) | 14, 51, 195 |
| PsuI | RGATCY | 1 cut(s) | 219 |
| PvuII | CAGCTG | 1 cut(s) | 110 |
| SaqAI | TTAA | 2 cut(s) | 131, 201 |
| SatI | GCNGC | 3 cut(s) | 13, 50, 194 |
| Sau3AI | GATC | 1 cut(s) | 219 |
| SetI | ASST | 6 cut(s) | 25, 54, 112, 156, 170, 181 |
| SfaNI | GCATC | 1 cut(s) | 35 |
| SgeI | CNNG | 5 cut(s) | 28, 35, 51, 70, 123 |
| SsiI | CCGC | 2 cut(s) | 12, 55 |
| SspMI | CTAG | 2 cut(s) | 39, 235 |
| TauI | GCSGC | 1 cut(s) | 15 |
| TfiI | GAWTC | 1 cut(s) | 35 |
| Tru1I | TTAA | 2 cut(s) | 131, 201 |
| Tru9I | TTAA | 2 cut(s) | 131, 201 |
| TseI | GCWGC | 2 cut(s) | 49, 193 |
| TspDTI | ATGAA | 1 cut(s) | 186 |
| TspGWI | ACGGA | 1 cut(s) | 107 |
| XspI | CTAG | 2 cut(s) | 39, 235 |