Rw6G033750

Cenp-O kinetochore centromere component

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001768
Physical Location & Seq
Forward (+)
58355763 .. 58357103
1341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G033750.1

Sequence Viewer

Length: 492 bp
ATGCCGCATAATAGTATTGTGATTATTGATATGAGTGTGCCGAATCAACGCCGACTCTGCACTTATGAATCCAAACTCCCAAATCCTTGCTTTGAAAGGGATCCACTGAATGTCCAAATATTCCTCAAAATGCATAGGTCTGATTATGCTAAAAGATGGCTGAAAGAAATGCAAAAGATTGATGAGGACCACACCTTAACTCAACTTGAAACAGCATGGCAAAATCTGGCTGTGGTAATTGTTAAGCTTAAGTATGCAGCTCTTGTTTCCATAATTCCAACAAATGTCAGTGTAGTAGTATGGCCCATTCAAATCTGGAGATTCAGTGTAAATATATCAACCATGAATACACTGGAAGGTCTAACTTGTCTACTCTATGCAGAGGATGCCTTACGAACCATGAGCTTACCTAAAGGTATACTTGTAATGTATGATGTTTTTCCTTGTTTAGTATTGTCAATAACAAACTATCTTGATGATATGAACAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CENP-O

Protein Analysis

163

Amino Acids

18.85

Weight (kDa)

6.27

Isoelectric Point (pI)

51.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Coatomer_E PF04733 34 - 86 3.5e-16 Coatomer epsilon subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 370, 418
AciI CCGC 1 cut(s) 5
AclWI GGATC 2 cut(s) 95, 108
AflII CTTAAG 1 cut(s) 248
AgsI TTSAA 3 cut(s) 95, 209, 311
AluBI AGCT 4 cut(s) 247, 260, 405, 489
AluI AGCT 4 cut(s) 247, 260, 405, 489
AlwI GGATC 2 cut(s) 95, 108
AoxI GGCC 1 cut(s) 302
ApeKI GCWGC 1 cut(s) 257
AspS9I GGNCC 2 cut(s) 187, 303
AvaII GGWCC 1 cut(s) 187
BamHI GGATCC 1 cut(s) 100
BbvI GCAGC 1 cut(s) 269
BccI CCATC 1 cut(s) 150
BfrI CTTAAG 1 cut(s) 248
BisI GCNGC 2 cut(s) 5, 258
BlsI GCNGC 2 cut(s) 6, 259
Bme18I GGWCC 1 cut(s) 187
BmgT120I GGNCC 2 cut(s) 187, 303
BmiI GGNNCC 1 cut(s) 102
BmsI GCATC 1 cut(s) 376
BpmI CTGGAG 1 cut(s) 337
BsaXI ACNNNNNCTCC 2 cut(s) 310, 340
Bse1I ACTGG 1 cut(s) 357
BseGI GGATG 1 cut(s) 391
BseNI ACTGG 1 cut(s) 357
BseXI GCAGC 1 cut(s) 269
BsgI GTGCAG 1 cut(s) 43
BshFI GGCC 1 cut(s) 304
BsnI GGCC 1 cut(s) 304
Bsp143I GATC 1 cut(s) 100
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 304
BspLI GGNNCC 1 cut(s) 102
BspPI GGATC 2 cut(s) 95, 108
BspTI CTTAAG 1 cut(s) 248
BsrI ACTGG 1 cut(s) 357
BssMI GATC 1 cut(s) 100
BssNAI GTATAC 1 cut(s) 419
Bst1107I GTATAC 1 cut(s) 419
BstAFI CTTAAG 1 cut(s) 248
BstAPI GCANNNNNTGC 1 cut(s) 386
BstF5I GGATG 1 cut(s) 391
BstKTI GATC 1 cut(s) 103
BstMBI GATC 1 cut(s) 100
BstMWI GCNNNNNNNGC 2 cut(s) 57, 386
BstV1I GCAGC 1 cut(s) 269
BstX2I RGATCY 1 cut(s) 100
BstYI RGATCY 1 cut(s) 100
BstZ17I GTATAC 1 cut(s) 419
BsuRI GGCC 1 cut(s) 304
BtsCI GGATG 1 cut(s) 391
BtsIMutI CAGTG 4 cut(s) 104, 295, 331, 350
Cfr13I GGNCC 2 cut(s) 187, 303
CviAII CATG 3 cut(s) 216, 343, 400
CviJI RGCY 7 cut(s) 160, 230, 247, 260, 304, 405, 489
CviKI_1 RGCY 7 cut(s) 160, 230, 247, 260, 304, 405, 489
DpnI GATC 1 cut(s) 102
DpnII GATC 1 cut(s) 100
Eco47I GGWCC 1 cut(s) 187
EcoT22I ATGCAT 1 cut(s) 135
FaeI CATG 3 cut(s) 219, 346, 403
FalI AAGNNNNNCTT 2 cut(s) 405, 437
FatI CATG 3 cut(s) 215, 342, 399
FblI GTMKAC 2 cut(s) 370, 418
Fnu4HI GCNGC 2 cut(s) 5, 258
FokI GGATG 1 cut(s) 398
Fsp4HI GCNGC 2 cut(s) 5, 258
GluI GCNGC 2 cut(s) 5, 258
GsuI CTGGAG 1 cut(s) 337
HaeIII GGCC 1 cut(s) 304
Hin1II CATG 3 cut(s) 219, 346, 403
HindIII AAGCTT 1 cut(s) 245
HinfI GANTC 4 cut(s) 43, 54, 68, 321
Hpy166II GTNNAC 2 cut(s) 371, 419
Hpy188I TCNGA 1 cut(s) 142
Hpy188III TCNNGA 2 cut(s) 316, 473
Hpy8I GTNNAC 2 cut(s) 371, 419
HpyAV CCTTC 1 cut(s) 350
HpyCH4V TGCA 5 cut(s) 60, 133, 172, 257, 380
HpyF10VI GCNNNNNNNGC 2 cut(s) 57, 386
Hsp92II CATG 3 cut(s) 219, 346, 403
Kzo9I GATC 1 cut(s) 100
LpnPI CCDG 3 cut(s) 212, 301, 338
Lsp1109I GCAGC 1 cut(s) 269
LweI GCATC 1 cut(s) 376
MalI GATC 1 cut(s) 102
MboI GATC 1 cut(s) 100
MflI RGATCY 1 cut(s) 100
MluCI AATT 2 cut(s) 237, 273
MlyI GAGTC 1 cut(s) 48
MmeI TCCRAC 1 cut(s) 302
MnlI CCTC 3 cut(s) 134, 178, 376
Mph1103I ATGCAT 1 cut(s) 135
MseI TTAA 3 cut(s) 197, 243, 249
MspA1I CMGCKG 1 cut(s) 489
MspCI CTTAAG 1 cut(s) 248
MwoI GCNNNNNNNGC 2 cut(s) 57, 386
NdeII GATC 1 cut(s) 100
NlaIII CATG 3 cut(s) 219, 346, 403
NlaIV GGNNCC 1 cut(s) 102
NsiI ATGCAT 1 cut(s) 135
PfeI GAWTC 3 cut(s) 43, 68, 321
PkrI GCNGC 2 cut(s) 6, 259
PleI GAGTC 1 cut(s) 48
PpsI GAGTC 1 cut(s) 48
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 2 cut(s) 187, 303
PsuI RGATCY 1 cut(s) 100
PvuII CAGCTG 1 cut(s) 489
SaqAI TTAA 3 cut(s) 197, 243, 249
SatI GCNGC 2 cut(s) 5, 258
Sau3AI GATC 1 cut(s) 100
Sau96I GGNCC 2 cut(s) 187, 303
SchI GAGTC 1 cut(s) 48
SetI ASST 9 cut(s) 140, 197, 249, 262, 361, 407, 412, 418, 491
SfaNI GCATC 1 cut(s) 376
SinI GGWCC 1 cut(s) 187
SmlI CTYRAG 1 cut(s) 248
SmoI CTYRAG 1 cut(s) 248
Sse9I AATT 2 cut(s) 237, 273
SsiI CCGC 1 cut(s) 5
SspI AATATT 1 cut(s) 120
TasI AATT 2 cut(s) 237, 273
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 3 cut(s) 43, 68, 321
Tru1I TTAA 3 cut(s) 197, 243, 249
Tru9I TTAA 3 cut(s) 197, 243, 249
TscAI CASTG 4 cut(s) 111, 295, 331, 357
TseI GCWGC 1 cut(s) 257
TspDTI ATGAA 2 cut(s) 81, 359
TspRI CASTG 4 cut(s) 111, 295, 331, 357
Vha464I CTTAAG 1 cut(s) 248
VpaK11BI GGWCC 1 cut(s) 187
XcmI CCANNNNNNNNNTGG 1 cut(s) 349
XmiI GTMKAC 2 cut(s) 370, 418
Zsp2I ATGCAT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.