RchiOBHm_Chr2g0102891

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001616
Physical Location & Seq
Reverse (-)
14522140 .. 14524960
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ47729

Sequence Viewer

Length: 606 bp
ATGCCGCCGTTGATGGAATACTTAGTCTCCATGCTCTCGGTTTTGAGGTGCGGTGAGGCGTTTCTGCCTCTGGACCCTTCTTGGCCAAAACAGAGGCTATTCTCCATCATTGTTTTGGCCAATGTTGACCTTATAATCACCTGCAGGACTCCGTTTGGGTACAAATTGGACTCCAATTGGCTCGAGCAAAGTTGCAATAGAAACTCTCTGTGGTTTTCCATAGGTGGAGAGTTTGTGACAGTGAGTGATGGAGGAGAGTGTAGGCGTGAGAGTGAGAAAGAGGATGAGAGGCTGTTCTGTTATGTAATGTACAGTTCGGGCTCGACGGGGAAGCCGAAAGGTGTTTGTGGCACTGAACAAGGTCTTTGGAATCGGTTTCTGTGGATGCAAGAATTGTATCCACTGAATGGAGAGGAGGTTTTGTTGTTCAAGACAGCAATAAGCTTTGTTGATCACTTTCAAGCATTTCTTAACGCTATTCTTACTGGTTTTGCTTTGGTTATACCTCCTTTCAATCAACTTAAACAGAATGTGTTCTCCATTGTGGATTTTCTACAGTTCTGGAGGTGTAGTCTTGGCTTCTCAAGATGGGAAGATTGTGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

201

Amino Acids

23.09

Weight (kDa)

4.98

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 1 - 175 9.4e-20 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 134
AarI CACCTGC 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 149
AccB7I CCANNNNNTGG 1 cut(s) 407
AciI CCGC 2 cut(s) 5, 51
AcoI YGGCCR 2 cut(s) 83, 117
AfaI GTAC 2 cut(s) 161, 311
AfiI CCNNNNNNNGG 1 cut(s) 407
AgsI TTSAA 3 cut(s) 430, 461, 514
AluBI AGCT 1 cut(s) 444
AluI AGCT 1 cut(s) 444
Alw26I GTCTC 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 2 cut(s) 83, 117
Asp700I GAANNNNTTC 1 cut(s) 533
AspS9I GGNCC 1 cut(s) 73
AsuHPI GGTGA 2 cut(s) 65, 130
AvaI CYCGRG 1 cut(s) 182
AvaII GGWCC 1 cut(s) 73
BalI TGGCCA 2 cut(s) 85, 119
BanII GRGCYC 1 cut(s) 323
BccI CCATC 4 cut(s) 7, 113, 242, 582
BciVI GTATCC 1 cut(s) 408
BclI TGATCA 1 cut(s) 451
BcoDI GTCTC 1 cut(s) 31
BfmI CTRYAG 2 cut(s) 142, 554
BfuAI ACCTGC 1 cut(s) 149
BfuI GTATCC 1 cut(s) 408
BisI GCNGC 1 cut(s) 5
BlsI GCNGC 1 cut(s) 6
Bme18I GGWCC 1 cut(s) 73
BmeT110I CYCGRG 1 cut(s) 182
BmgT120I GGNCC 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 75
BmsI GCATC 1 cut(s) 375
BpmI CTGGAG 1 cut(s) 583
BpuEI CTTGAG 1 cut(s) 568
Bsc4I CCNNNNNNNGG 1 cut(s) 407
Bse1I ACTGG 1 cut(s) 490
BseGI GGATG 2 cut(s) 289, 390
BseLI CCNNNNNNNGG 1 cut(s) 407
BseNI ACTGG 1 cut(s) 490
BseRI GAGGAG 2 cut(s) 267, 428
BshFI GGCC 2 cut(s) 85, 119
BsiHKCI CYCGRG 1 cut(s) 182
BslI CCNNNNNNNGG 1 cut(s) 407
BsmAI GTCTC 1 cut(s) 31
BsnI GGCC 2 cut(s) 85, 119
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 323
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 1 cut(s) 451
BspACI CCGC 2 cut(s) 5, 51
BspANI GGCC 2 cut(s) 85, 119
BspLI GGNNCC 1 cut(s) 75
BspMAI CTGCAG 1 cut(s) 146
BspMI ACCTGC 1 cut(s) 149
BsrGI TGTACA 1 cut(s) 309
BsrI ACTGG 1 cut(s) 490
BssMI GATC 1 cut(s) 451
Bst4CI ACNGT 3 cut(s) 241, 314, 558
BstAUI TGTACA 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 22
BstF5I GGATG 2 cut(s) 289, 390
BstKTI GATC 1 cut(s) 454
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 1 cut(s) 451
BstSFI CTRYAG 2 cut(s) 142, 554
BsuI GTATCC 1 cut(s) 408
BsuRI GGCC 2 cut(s) 85, 119
BtsCI GGATG 2 cut(s) 289, 390
BtsIMutI CAGTG 3 cut(s) 246, 351, 401
BveI ACCTGC 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 73
Csp6I GTAC 2 cut(s) 160, 310
CviAII CATG 1 cut(s) 31
CviJI RGCY 9 cut(s) 85, 97, 119, 181, 292, 321, 334, 444, 579
CviKI_1 RGCY 9 cut(s) 85, 97, 119, 181, 292, 321, 334, 444, 579
CviQI GTAC 2 cut(s) 160, 310
DdeI CTNAG 1 cut(s) 22
DpnI GATC 1 cut(s) 453
DpnII GATC 1 cut(s) 451
EaeI YGGCCR 2 cut(s) 83, 117
Eco24I GRGCYC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 182
EcoT38I GRGCYC 1 cut(s) 323
FaeI CATG 1 cut(s) 34
FaiI YATR 5 cut(s) 32, 134, 221, 303, 503
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FatI CATG 1 cut(s) 30
FbaI TGATCA 1 cut(s) 451
Fnu4HI GCNGC 1 cut(s) 5
FokI GGATG 2 cut(s) 296, 397
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 1 cut(s) 5
GluI GCNGC 1 cut(s) 5
GsuI CTGGAG 1 cut(s) 583
HaeIII GGCC 2 cut(s) 85, 119
Hin1II CATG 1 cut(s) 34
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 442
HinfI GANTC 3 cut(s) 148, 170, 370
HphI GGTGA 2 cut(s) 65, 130
Hpy166II GTNNAC 1 cut(s) 127
Hpy188III TCNNGA 4 cut(s) 71, 430, 562, 585
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 1 cut(s) 87
HpyCH4III ACNGT 3 cut(s) 241, 314, 558
HpyCH4V TGCA 3 cut(s) 144, 195, 388
HpyF3I CTNAG 1 cut(s) 22
Hsp92II CATG 1 cut(s) 34
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 1 cut(s) 451
LpnPI CCDG 5 cut(s) 56, 130, 154, 471, 547
LweI GCATC 1 cut(s) 375
MaeIII GTNAC 1 cut(s) 235
MalI GATC 1 cut(s) 453
MboI GATC 1 cut(s) 451
MboII GAAGA 1 cut(s) 605
MfeI CAATTG 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 323
MlsI TGGCCA 2 cut(s) 85, 119
MluCI AATT 3 cut(s) 164, 175, 392
MluNI TGGCCA 2 cut(s) 85, 119
MlyI GAGTC 2 cut(s) 142, 164
Mox20I TGGCCA 2 cut(s) 85, 119
MroXI GAANNNNTTC 1 cut(s) 533
MscI TGGCCA 2 cut(s) 85, 119
MseI TTAA 2 cut(s) 471, 522
Msp20I TGGCCA 2 cut(s) 85, 119
MunI CAATTG 1 cut(s) 175
NdeII GATC 1 cut(s) 451
NlaIII CATG 1 cut(s) 34
NlaIV GGNNCC 1 cut(s) 75
NmuCI GTSAC 1 cut(s) 235
PaeR7I CTCGAG 1 cut(s) 182
PaqCI CACCTGC 1 cut(s) 149
PcsI WCGNNNNNNNCGW 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 533
PfeI GAWTC 1 cut(s) 370
PflMI CCANNNNNTGG 1 cut(s) 407
PkrI GCNGC 1 cut(s) 6
PleI GAGTC 2 cut(s) 142, 164
PpsI GAGTC 2 cut(s) 142, 164
PsiI TTATAA 1 cut(s) 134
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 1 cut(s) 73
PspXI VCTCGAGB 1 cut(s) 182
PstI CTGCAG 1 cut(s) 146
RsaI GTAC 2 cut(s) 161, 311
RsaNI GTAC 2 cut(s) 160, 310
SaqAI TTAA 2 cut(s) 471, 522
SatI GCNGC 1 cut(s) 5
Sau3AI GATC 1 cut(s) 451
Sau96I GGNCC 1 cut(s) 73
SbfI CCTGCAGG 1 cut(s) 146
SchI GAGTC 2 cut(s) 142, 164
SdaI CCTGCAGG 1 cut(s) 146
SduI GDGCHC 1 cut(s) 323
SfaNI GCATC 1 cut(s) 375
SfcI CTRYAG 2 cut(s) 142, 554
Sfr274I CTCGAG 1 cut(s) 182
SinI GGWCC 1 cut(s) 73
SlaI CTCGAG 1 cut(s) 182
SmlI CTYRAG 2 cut(s) 182, 583
SmoI CTYRAG 2 cut(s) 182, 583
Sse8387I CCTGCAGG 1 cut(s) 146
Sse9I AATT 3 cut(s) 164, 175, 392
SsiI CCGC 2 cut(s) 5, 51
TaaI ACNGT 3 cut(s) 241, 314, 558
TaqI TCGA 2 cut(s) 183, 323
TasI AATT 3 cut(s) 164, 175, 392
TatI WGTACW 1 cut(s) 309
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 2 cut(s) 471, 522
Tru9I TTAA 2 cut(s) 471, 522
TscAI CASTG 3 cut(s) 246, 358, 408
TseFI GTSAC 1 cut(s) 235
Tsp45I GTSAC 1 cut(s) 235
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 3 cut(s) 246, 358, 408
Van91I CCANNNNNTGG 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 73
XcmI CCANNNNNNNNNTGG 1 cut(s) 112
XhoI CTCGAG 1 cut(s) 182
XmnI GAANNNNTTC 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.