RLG00000010663

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001616
Physical Location & Seq
Reverse (-)
3417334 .. 3419059
1726 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000010663

Sequence Viewer

Length: 729 bp
ATGGAATACATAGTCTCCGTGCTCTCGGTTTTGAGGTGCGGTGAGGCTTTTCTGCCTCTGGACCCTTCCTGGCCAAAACAGAGGCTATTCTCCGTCATTGCTTTGGCCAATGTCGACTTAATAATCACCTGCAGGACTCCGTTTGGGTACAAATTGGACTCCAATTGGCTCGAGCAGAGTTGCAATCGAAATTCTCTGTGGTTTTCCATAGGTGGAGAGTTTGTGACAGTGAGTGATGGAGAAGAGTGTAGGCGTAAGAGTGAGAAAGAGGATGAGAGGCTGTTCTGTTATGTAATGTACAGTTCGGGCTCGACGGGGAAGCCGAAAGGTGTTTGTGGCACTGAACAAGGTCTTTGGAATCGGTTTCTGTGGATGCAAGAATTGTATCCACTGAATGGAGAGGAGGTTTTGTTGTTCAAGACAGCAATAAGCTTTGTTGATCACTGTCAAGAATTTCTTAACGCTATTCTTACTGGTTCTGCTTTGGTTATACCTCCTTTTAATCAACTTAAACAGAATGTGTTCTGCATTGTGGATTTTCTACAGCTAATGTGCTATTTCGTGACAGGAGATAGAACTAATGTAATGTGGAACCTGAATAAGCTGCAACACATCCAGAATTTAAATTATTCCTATTCTGGAGGTGTAGTCTTGGCTTCTCGAGATGGGAAGATTGTGCGTGACAACACTCTCGATGCACGGTTGGATGTTGTATTCAGTATTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

243

Amino Acids

27.6

Weight (kDa)

5.3

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 2 - 180 2.2e-19 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 395
AccI GTMKAC 1 cut(s) 114
AciI CCGC 1 cut(s) 39
AcoI YGGCCR 2 cut(s) 71, 105
AcsI RAATTY 3 cut(s) 190, 452, 619
AfaI GTAC 2 cut(s) 149, 299
AfiI CCNNNNNNNGG 1 cut(s) 395
AgsI TTSAA 1 cut(s) 418
AjnI CCWGG 1 cut(s) 68
AluBI AGCT 3 cut(s) 432, 547, 604
AluI AGCT 3 cut(s) 432, 547, 604
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 1 cut(s) 19
Ama87I CYCGRG 2 cut(s) 170, 660
AoxI GGCC 2 cut(s) 71, 105
ApeKI GCWGC 1 cut(s) 604
ApoI RAATTY 3 cut(s) 190, 452, 619
Asp700I GAANNNNTTC 1 cut(s) 521
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 2 cut(s) 53, 118
AvaI CYCGRG 2 cut(s) 170, 660
AvaII GGWCC 1 cut(s) 61
BalI TGGCCA 2 cut(s) 73, 107
BanII GRGCYC 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 1 cut(s) 591
BccI CCATC 2 cut(s) 230, 659
BciT130I CCWGG 1 cut(s) 70
BciVI GTATCC 1 cut(s) 396
BclI TGATCA 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 19
BfmI CTRYAG 2 cut(s) 130, 542
BfuAI ACCTGC 1 cut(s) 137
BfuI GTATCC 1 cut(s) 396
BisI GCNGC 1 cut(s) 605
BlsI GCNGC 1 cut(s) 606
Bme1390I CCNGG 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 2 cut(s) 170, 660
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 2 cut(s) 63, 593
BmrFI CCNGG 1 cut(s) 70
BmsI GCATC 2 cut(s) 363, 685
BpmI CTGGAG 1 cut(s) 660
BsaXI ACNNNNNCTCC 9 cut(s) 29, 207, 237, 390, 395, 420, 425, 633, 663
Bsc4I CCNNNNNNNGG 1 cut(s) 395
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 70
BseGI GGATG 4 cut(s) 277, 378, 612, 712
BseLI CCNNNNNNNGG 1 cut(s) 395
BseMI GCAATG 1 cut(s) 96
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 1 cut(s) 416
BseXI GCAGC 1 cut(s) 591
BshFI GGCC 2 cut(s) 73, 107
BsiHKAI GWGCWC 1 cut(s) 24
BsiHKCI CYCGRG 2 cut(s) 170, 660
BslI CCNNNNNNNGG 1 cut(s) 395
BsmAI GTCTC 1 cut(s) 19
BsnI GGCC 2 cut(s) 73, 107
BsoBI CYCGRG 2 cut(s) 170, 660
Bsp1286I GDGCHC 2 cut(s) 24, 311
Bsp1407I TGTACA 1 cut(s) 297
Bsp143I GATC 1 cut(s) 439
BspACI CCGC 1 cut(s) 39
BspANI GGCC 2 cut(s) 73, 107
BspLI GGNNCC 2 cut(s) 63, 593
BspMAI CTGCAG 1 cut(s) 134
BspMI ACCTGC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 96
BsrGI TGTACA 1 cut(s) 297
BsrI ACTGG 1 cut(s) 478
BssMI GATC 1 cut(s) 439
Bst2UI CCWGG 1 cut(s) 70
Bst4CI ACNGT 4 cut(s) 229, 302, 446, 702
Bst6I CTCTTC 1 cut(s) 237
BstAUI TGTACA 1 cut(s) 297
BstF5I GGATG 4 cut(s) 277, 378, 612, 712
BstKTI GATC 1 cut(s) 442
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 1 cut(s) 439
BstNI CCWGG 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 68
BstSFI CTRYAG 2 cut(s) 130, 542
BstV1I GCAGC 1 cut(s) 591
BsuI GTATCC 1 cut(s) 396
BsuRI GGCC 2 cut(s) 73, 107
BtsCI GGATG 4 cut(s) 277, 378, 612, 712
BtsIMutI CAGTG 4 cut(s) 234, 339, 389, 442
BveI ACCTGC 1 cut(s) 137
Cfr13I GGNCC 1 cut(s) 61
Csp6I GTAC 2 cut(s) 148, 298
CviQI GTAC 2 cut(s) 148, 298
DpnI GATC 1 cut(s) 441
DpnII GATC 1 cut(s) 439
DraI TTTAAA 1 cut(s) 624
EaeI YGGCCR 2 cut(s) 71, 105
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Eco24I GRGCYC 1 cut(s) 311
Eco47I GGWCC 1 cut(s) 61
Eco88I CYCGRG 2 cut(s) 170, 660
EcoRII CCWGG 1 cut(s) 68
EcoT38I GRGCYC 1 cut(s) 311
FaiI YATR 4 cut(s) 11, 209, 291, 491
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FbaI TGATCA 1 cut(s) 439
FblI GTMKAC 1 cut(s) 114
Fnu4HI GCNGC 1 cut(s) 605
FokI GGATG 4 cut(s) 284, 385, 599, 719
FriOI GRGCYC 1 cut(s) 311
Fsp4HI GCNGC 1 cut(s) 605
GluI GCNGC 1 cut(s) 605
GsuI CTGGAG 1 cut(s) 660
HaeIII GGCC 2 cut(s) 73, 107
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 430
HinfI GANTC 3 cut(s) 136, 158, 358
HphI GGTGA 2 cut(s) 53, 118
Hpy166II GTNNAC 1 cut(s) 115
Hpy188III TCNNGA 9 cut(s) 59, 418, 449, 562, 616, 639, 660, 662, 692
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 316
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 4 cut(s) 229, 302, 446, 702
HpyCH4V TGCA 6 cut(s) 132, 183, 376, 528, 607, 698
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 1 cut(s) 439
Lsp1109I GCAGC 1 cut(s) 591
LweI GCATC 2 cut(s) 363, 685
MaeIII GTNAC 3 cut(s) 223, 562, 680
MalI GATC 1 cut(s) 441
MboI GATC 1 cut(s) 439
MboII GAAGA 2 cut(s) 254, 682
MfeI CAATTG 1 cut(s) 163
MhlI GDGCHC 2 cut(s) 24, 311
MlsI TGGCCA 2 cut(s) 73, 107
MluCI AATT 7 cut(s) 152, 163, 190, 380, 452, 619, 625
MluNI TGGCCA 2 cut(s) 73, 107
MlyI GAGTC 2 cut(s) 130, 152
MmeI TCCRAC 1 cut(s) 684
Mox20I TGGCCA 2 cut(s) 73, 107
MroXI GAANNNNTTC 1 cut(s) 521
MscI TGGCCA 2 cut(s) 73, 107
MseI TTAA 5 cut(s) 119, 459, 501, 510, 623
Msp20I TGGCCA 2 cut(s) 73, 107
MspR9I CCNGG 1 cut(s) 70
MunI CAATTG 1 cut(s) 163
MvaI CCWGG 1 cut(s) 70
NdeII GATC 1 cut(s) 439
NlaIV GGNNCC 2 cut(s) 63, 593
NmuCI GTSAC 3 cut(s) 223, 562, 680
PaeR7I CTCGAG 2 cut(s) 170, 660
PaqCI CACCTGC 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 320
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 1 cut(s) 358
PflMI CCANNNNNTGG 1 cut(s) 395
PkrI GCNGC 1 cut(s) 606
PleI GAGTC 2 cut(s) 130, 152
PpsI GAGTC 2 cut(s) 130, 152
Psp6I CCWGG 1 cut(s) 68
PspGI CCWGG 1 cut(s) 68
PspN4I GGNNCC 2 cut(s) 63, 593
PspPI GGNCC 1 cut(s) 61
PspXI VCTCGAGB 1 cut(s) 170
PstI CTGCAG 1 cut(s) 134
RsaI GTAC 2 cut(s) 149, 299
RsaNI GTAC 2 cut(s) 148, 298
SalI GTCGAC 1 cut(s) 113
SaqAI TTAA 5 cut(s) 119, 459, 501, 510, 623
SatI GCNGC 1 cut(s) 605
Sau3AI GATC 1 cut(s) 439
Sau96I GGNCC 1 cut(s) 61
SbfI CCTGCAGG 1 cut(s) 134
SchI GAGTC 2 cut(s) 130, 152
ScrFI CCNGG 1 cut(s) 70
SdaI CCTGCAGG 1 cut(s) 134
SduI GDGCHC 2 cut(s) 24, 311
SfaNI GCATC 2 cut(s) 363, 685
SfcI CTRYAG 2 cut(s) 130, 542
Sfr274I CTCGAG 2 cut(s) 170, 660
SinI GGWCC 1 cut(s) 61
SlaI CTCGAG 2 cut(s) 170, 660
SmiI ATTTAAAT 1 cut(s) 624
SmlI CTYRAG 2 cut(s) 170, 660
SmoI CTYRAG 2 cut(s) 170, 660
Sse8387I CCTGCAGG 1 cut(s) 134
Sse9I AATT 7 cut(s) 152, 163, 190, 380, 452, 619, 625
SsiI CCGC 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 68
SwaI ATTTAAAT 1 cut(s) 624
TaaI ACNGT 4 cut(s) 229, 302, 446, 702
TaqI TCGA 6 cut(s) 114, 171, 187, 311, 661, 693
TasI AATT 7 cut(s) 152, 163, 190, 380, 452, 619, 625
TatI WGTACW 1 cut(s) 297
TfiI GAWTC 1 cut(s) 358
Tru1I TTAA 5 cut(s) 119, 459, 501, 510, 623
Tru9I TTAA 5 cut(s) 119, 459, 501, 510, 623
TscAI CASTG 4 cut(s) 234, 346, 396, 449
TseFI GTSAC 3 cut(s) 223, 562, 680
TseI GCWGC 1 cut(s) 604
Tsp45I GTSAC 3 cut(s) 223, 562, 680
TspGWI ACGGA 3 cut(s) 7, 82, 129
TspRI CASTG 4 cut(s) 234, 346, 396, 449
Van91I CCANNNNNTGG 1 cut(s) 395
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 3 cut(s) 190, 452, 619
XhoI CTCGAG 2 cut(s) 170, 660
XmiI GTMKAC 1 cut(s) 114
XmnI GAANNNNTTC 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.