Rmu_sc0004031.1_g000010

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004031.1
OG0001616
Physical Location & Seq
Forward (+)
47295 .. 49497
2203 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004031.1_g000010.1.cds

Sequence Viewer

Length: 780 bp
atgaagacgtggcggctgacgaatacaatgacatttccggcgagatgttcgactggctccggcgagatgttcgacgggctcgaacaaagcgacgatgaaattgaaatggagttgtctgtggctgattcagagtcggtgattcgggatgaggaattcaggtggcagagagtggaaaagctctgcgaagatgtgaagcagttcggcgaggaaatgattgacgatggctctctcgcctctatctacgatttccgaatcgataaatttcagaggctattctccgtcattgttttggccaatgttgacctaataatcacctgcagtactccatttgggtacaaattggactccaattggctcgagcagagttgcaatcgaaattctctgtggttttccataggtggagagtttgtgacagtgagtgatgcaggagagtgtaggcgtgagagtgagaaagaggatgagaggttgttctattatgtaatgtacagttcgggctcgacggggaagccgaaaggtatttgtggcactgaacaaggtctttggaatcggtttctgtggatgcaagaattgtatccactgaatggagaggaggttttgttgttcaagacagcaataagctttgttgatcactttcaagcatttcttaacgctattcttactggttctgctttggttatacctcctttcaatcaacttaaacagaatgtgttctccattgtggattttctacagttctggaggtgtagtcttggcttctcgagatgggaagattgtgcgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

259

Amino Acids

29.89

Weight (kDa)

4.49

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 323
Acc36I ACCTGC 1 cut(s) 323
AccB7I CCANNNNNTGG 1 cut(s) 581
AciI CCGC 1 cut(s) 13
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 3 cut(s) 152, 260, 376
AfaI GTAC 3 cut(s) 322, 335, 485
AfiI CCNNNNNNNGG 1 cut(s) 581
AgsI TTSAA 4 cut(s) 104, 604, 635, 688
AjiI CACGTC 1 cut(s) 9
AluBI AGCT 2 cut(s) 178, 618
AluI AGCT 2 cut(s) 178, 618
Ama87I CYCGRG 2 cut(s) 356, 757
AoxI GGCC 1 cut(s) 291
ApoI RAATTY 3 cut(s) 152, 260, 376
Asp700I GAANNNNTTC 2 cut(s) 197, 707
AsuHPI GGTGA 2 cut(s) 148, 304
AvaI CYCGRG 2 cut(s) 356, 757
BalI TGGCCA 1 cut(s) 293
BanII GRGCYC 2 cut(s) 81, 497
BbsI GAAGAC 1 cut(s) 11
BccI CCATC 2 cut(s) 215, 756
BciVI GTATCC 1 cut(s) 582
BclI TGATCA 1 cut(s) 625
BfmI CTRYAG 2 cut(s) 316, 728
BfuAI ACCTGC 1 cut(s) 323
BfuI GTATCC 1 cut(s) 582
BisI GCNGC 1 cut(s) 14
BlsI GCNGC 1 cut(s) 15
BmcAI AGTACT 1 cut(s) 322
BmeT110I CYCGRG 2 cut(s) 356, 757
BmgBI CACGTC 1 cut(s) 9
BmiI GGNNCC 1 cut(s) 58
BmsI GCATC 2 cut(s) 412, 549
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 757
Bsa29I ATCGAT 1 cut(s) 255
BsaXI ACNNNNNCTCC 8 cut(s) 393, 423, 576, 581, 606, 611, 730, 760
Bsc4I CCNNNNNNNGG 1 cut(s) 581
Bse1I ACTGG 2 cut(s) 58, 664
BseCI ATCGAT 1 cut(s) 255
BseGI GGATG 3 cut(s) 151, 463, 564
BseLI CCNNNNNNNGG 1 cut(s) 581
BseNI ACTGG 2 cut(s) 58, 664
BseRI GAGGAG 1 cut(s) 602
BshFI GGCC 1 cut(s) 293
BshVI ATCGAT 1 cut(s) 255
BsiHKCI CYCGRG 2 cut(s) 356, 757
BsiSI CCGG 2 cut(s) 38, 60
BslI CCNNNNNNNGG 1 cut(s) 581
BsnI GGCC 1 cut(s) 293
BsoBI CYCGRG 2 cut(s) 356, 757
Bsp1286I GDGCHC 2 cut(s) 81, 497
Bsp1407I TGTACA 1 cut(s) 483
Bsp143I GATC 1 cut(s) 625
BspACI CCGC 1 cut(s) 13
BspANI GGCC 1 cut(s) 293
BspDI ATCGAT 1 cut(s) 255
BspLI GGNNCC 1 cut(s) 58
BspMAI CTGCAG 1 cut(s) 320
BspMI ACCTGC 1 cut(s) 323
BsrGI TGTACA 1 cut(s) 483
BsrI ACTGG 2 cut(s) 58, 664
BssMI GATC 1 cut(s) 625
Bst4CI ACNGT 3 cut(s) 415, 488, 732
BstAUI TGTACA 1 cut(s) 483
BstF5I GGATG 3 cut(s) 151, 463, 564
BstKTI GATC 1 cut(s) 628
BstMBI GATC 1 cut(s) 625
BstSFI CTRYAG 2 cut(s) 316, 728
BstV2I GAAGAC 1 cut(s) 11
Bsu15I ATCGAT 1 cut(s) 255
BsuI GTATCC 1 cut(s) 582
BsuRI GGCC 1 cut(s) 293
BsuTUI ATCGAT 1 cut(s) 255
BtrI CACGTC 1 cut(s) 9
BtsCI GGATG 3 cut(s) 151, 463, 564
BtsIMutI CAGTG 3 cut(s) 420, 525, 575
BveI ACCTGC 1 cut(s) 323
ClaI ATCGAT 1 cut(s) 255
Csp6I GTAC 3 cut(s) 321, 334, 484
CviQI GTAC 3 cut(s) 321, 334, 484
DpnI GATC 1 cut(s) 627
DpnII GATC 1 cut(s) 625
EaeI YGGCCR 1 cut(s) 291
Eco24I GRGCYC 2 cut(s) 81, 497
Eco88I CYCGRG 2 cut(s) 356, 757
EcoRI GAATTC 1 cut(s) 152
EcoT38I GRGCYC 2 cut(s) 81, 497
FaiI YATR 3 cut(s) 395, 477, 677
FalI AAGNNNNNCTT 2 cut(s) 627, 659
FbaI TGATCA 1 cut(s) 625
Fnu4HI GCNGC 1 cut(s) 14
FokI GGATG 3 cut(s) 158, 470, 571
FriOI GRGCYC 2 cut(s) 81, 497
Fsp4HI GCNGC 1 cut(s) 14
GluI GCNGC 1 cut(s) 14
GsuI CTGGAG 1 cut(s) 757
HaeIII GGCC 1 cut(s) 293
HapII CCGG 2 cut(s) 38, 60
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HindIII AAGCTT 1 cut(s) 616
HinfI GANTC 6 cut(s) 125, 131, 139, 252, 344, 544
HpaII CCGG 2 cut(s) 38, 60
HphI GGTGA 2 cut(s) 148, 304
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 3 cut(s) 130, 251, 267
Hpy188III TCNNGA 5 cut(s) 143, 604, 736, 757, 759
Hpy8I GTNNAC 1 cut(s) 301
Hpy99I CGWCG 3 cut(s) 77, 95, 502
HpyCH4III ACNGT 3 cut(s) 415, 488, 732
HpyCH4IV ACGT 1 cut(s) 8
HpyCH4V TGCA 4 cut(s) 318, 369, 425, 562
HpySE526I ACGT 1 cut(s) 8
Ksp22I TGATCA 1 cut(s) 625
Kzo9I GATC 1 cut(s) 625
LmnI GCTCC 1 cut(s) 62
LpnPI CCDG 8 cut(s) 39, 51, 73, 142, 328, 411, 645, 721
LweI GCATC 2 cut(s) 412, 549
MaeII ACGT 1 cut(s) 8
MaeIII GTNAC 1 cut(s) 409
MalI GATC 1 cut(s) 627
MboI GATC 1 cut(s) 625
MboII GAAGA 3 cut(s) 16, 197, 779
MfeI CAATTG 1 cut(s) 349
MhlI GDGCHC 2 cut(s) 81, 497
MlsI TGGCCA 1 cut(s) 293
MluCI AATT 7 cut(s) 99, 152, 260, 338, 349, 376, 566
MluNI TGGCCA 1 cut(s) 293
MlyI GAGTC 2 cut(s) 140, 338
Mox20I TGGCCA 1 cut(s) 293
MroXI GAANNNNTTC 2 cut(s) 197, 707
MscI TGGCCA 1 cut(s) 293
MseI TTAA 2 cut(s) 645, 696
Msp20I TGGCCA 1 cut(s) 293
MspI CCGG 2 cut(s) 38, 60
MunI CAATTG 1 cut(s) 349
NdeII GATC 1 cut(s) 625
NlaIV GGNNCC 1 cut(s) 58
NmuCI GTSAC 1 cut(s) 409
PaeR7I CTCGAG 2 cut(s) 356, 757
PaqCI CACCTGC 1 cut(s) 323
PcsI WCGNNNNNNNCGW 3 cut(s) 78, 87, 506
PdmI GAANNNNTTC 2 cut(s) 197, 707
PfeI GAWTC 4 cut(s) 125, 139, 252, 544
PflMI CCANNNNNTGG 1 cut(s) 581
PkrI GCNGC 1 cut(s) 15
PleI GAGTC 2 cut(s) 139, 338
PpsI GAGTC 2 cut(s) 139, 338
PspN4I GGNNCC 1 cut(s) 58
PspXI VCTCGAGB 1 cut(s) 356
PstI CTGCAG 1 cut(s) 320
RsaI GTAC 3 cut(s) 322, 335, 485
RsaNI GTAC 3 cut(s) 321, 334, 484
SaqAI TTAA 2 cut(s) 645, 696
SatI GCNGC 1 cut(s) 14
Sau3AI GATC 1 cut(s) 625
ScaI AGTACT 1 cut(s) 322
SchI GAGTC 2 cut(s) 140, 338
SduI GDGCHC 2 cut(s) 81, 497
SfaNI GCATC 2 cut(s) 412, 549
SfcI CTRYAG 2 cut(s) 316, 728
Sfr274I CTCGAG 2 cut(s) 356, 757
SlaI CTCGAG 2 cut(s) 356, 757
SmlI CTYRAG 2 cut(s) 356, 757
SmoI CTYRAG 2 cut(s) 356, 757
Sse9I AATT 7 cut(s) 99, 152, 260, 338, 349, 376, 566
SsiI CCGC 1 cut(s) 13
TaaI ACNGT 3 cut(s) 415, 488, 732
TaiI ACGT 1 cut(s) 11
TaqI TCGA 8 cut(s) 50, 72, 81, 255, 357, 373, 497, 758
TasI AATT 7 cut(s) 99, 152, 260, 338, 349, 376, 566
TatI WGTACW 2 cut(s) 320, 483
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 4 cut(s) 125, 139, 252, 544
Tru1I TTAA 2 cut(s) 645, 696
Tru9I TTAA 2 cut(s) 645, 696
TscAI CASTG 3 cut(s) 420, 532, 582
TseFI GTSAC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 2 cut(s) 17, 111
TspGWI ACGGA 1 cut(s) 268
TspRI CASTG 3 cut(s) 420, 532, 582
Van91I CCANNNNNTGG 1 cut(s) 581
XapI RAATTY 3 cut(s) 152, 260, 376
XhoI CTCGAG 2 cut(s) 356, 757
XmnI GAANNNNTTC 2 cut(s) 197, 707
ZrmI AGTACT 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.