RLG00000003325

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001616
Physical Location & Seq
Reverse (-)
47074701 .. 47075246
546 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003325

Sequence Viewer

Length: 546 bp
ATGGAATACATAGTCTCCGTGCTCTCGGTTTTGAGGTACGGTGAGGCGTTTCTGCCTCTGGACCCTTCCTGGCCAAAACAGAGGCTATTCTCCGTCATTGCTTTGGCCAATGTCGACCTAATAATCACCTGCAGGACTCCGTTTGGGTACAAATTGGACTCCAATTGGCTTGAGCAAAGTTGCAATCGAAATTCTCTGTGGTTTTCCATAGGTGGAGAGTTTGTAACAGTGAGTGATGGAGAAGAGTGTAGGCGTGAGAGTGAGAAAGAGGATGAGAGGCTGTTCTGTTATGTAATGTACAGTTCGGGCTCGACGGGGAAGCCGAAAGGTGTTTGTGGCACTGAACAAGGTCTTTGGAATCGGTTTCTGTGGATGCAAGAATTGTATCCACTGAATGGAGAGGAGGTTTTGTTGTTCAAGACAGCAATAAGCTTTGTTGATCACTTTCAAGAATTTCTTAACGCTATTCTTACTGGTTCTGCTTTGGTTATACCTCCTTTCAATCAACTTAAACAGAATGTGTTCTCTATTGTGGATTTTCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

182

Amino Acids

20.73

Weight (kDa)

4.77

Isoelectric Point (pI)

39.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 2 - 171 2.2e-19 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 395
AccI GTMKAC 1 cut(s) 114
AcoI YGGCCR 2 cut(s) 71, 105
AcsI RAATTY 2 cut(s) 190, 452
AfaI GTAC 3 cut(s) 38, 149, 299
AfiI CCNNNNNNNGG 1 cut(s) 395
AgsI TTSAA 3 cut(s) 418, 449, 502
AjnI CCWGG 1 cut(s) 68
AluBI AGCT 1 cut(s) 432
AluI AGCT 1 cut(s) 432
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 1 cut(s) 19
AoxI GGCC 2 cut(s) 71, 105
ApoI RAATTY 2 cut(s) 190, 452
Asp700I GAANNNNTTC 1 cut(s) 521
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 2 cut(s) 53, 118
AvaII GGWCC 1 cut(s) 61
BalI TGGCCA 2 cut(s) 73, 107
BanII GRGCYC 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 24
BccI CCATC 1 cut(s) 230
BciT130I CCWGG 1 cut(s) 70
BciVI GTATCC 1 cut(s) 396
BclI TGATCA 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 19
BfmI CTRYAG 2 cut(s) 130, 542
BfuAI ACCTGC 1 cut(s) 137
BfuI GTATCC 1 cut(s) 396
Bme1390I CCNGG 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 70
BmsI GCATC 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 191
BsaXI ACNNNNNCTCC 7 cut(s) 29, 207, 237, 390, 395, 420, 425
Bsc4I CCNNNNNNNGG 1 cut(s) 395
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 70
BseGI GGATG 2 cut(s) 277, 378
BseLI CCNNNNNNNGG 1 cut(s) 395
BseMI GCAATG 1 cut(s) 96
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 1 cut(s) 416
BshFI GGCC 2 cut(s) 73, 107
BsiHKAI GWGCWC 1 cut(s) 24
BslI CCNNNNNNNGG 1 cut(s) 395
BsmAI GTCTC 1 cut(s) 19
BsnI GGCC 2 cut(s) 73, 107
Bsp1286I GDGCHC 2 cut(s) 24, 311
Bsp1407I TGTACA 1 cut(s) 297
Bsp143I GATC 1 cut(s) 439
BspANI GGCC 2 cut(s) 73, 107
BspLI GGNNCC 1 cut(s) 63
BspMAI CTGCAG 1 cut(s) 134
BspMI ACCTGC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 96
BsrGI TGTACA 1 cut(s) 297
BsrI ACTGG 1 cut(s) 478
BssMI GATC 1 cut(s) 439
Bst2UI CCWGG 1 cut(s) 70
Bst4CI ACNGT 3 cut(s) 41, 229, 302
Bst6I CTCTTC 1 cut(s) 237
BstAUI TGTACA 1 cut(s) 297
BstF5I GGATG 2 cut(s) 277, 378
BstKTI GATC 1 cut(s) 442
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 1 cut(s) 439
BstNI CCWGG 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 68
BstSFI CTRYAG 2 cut(s) 130, 542
BsuI GTATCC 1 cut(s) 396
BsuRI GGCC 2 cut(s) 73, 107
BtsCI GGATG 2 cut(s) 277, 378
BtsIMutI CAGTG 3 cut(s) 234, 339, 389
BveI ACCTGC 1 cut(s) 137
Cfr13I GGNCC 1 cut(s) 61
Csp6I GTAC 3 cut(s) 37, 148, 298
CviJI RGCY 8 cut(s) 73, 85, 107, 169, 280, 309, 322, 432
CviKI_1 RGCY 8 cut(s) 73, 85, 107, 169, 280, 309, 322, 432
CviQI GTAC 3 cut(s) 37, 148, 298
DpnI GATC 1 cut(s) 441
DpnII GATC 1 cut(s) 439
EaeI YGGCCR 2 cut(s) 71, 105
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Eco24I GRGCYC 1 cut(s) 311
Eco47I GGWCC 1 cut(s) 61
EcoRII CCWGG 1 cut(s) 68
EcoT38I GRGCYC 1 cut(s) 311
FaiI YATR 5 cut(s) 11, 209, 291, 491, 544
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FbaI TGATCA 1 cut(s) 439
FblI GTMKAC 1 cut(s) 114
FokI GGATG 2 cut(s) 284, 385
FriOI GRGCYC 1 cut(s) 311
HaeIII GGCC 2 cut(s) 73, 107
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 430
HinfI GANTC 3 cut(s) 136, 158, 358
HphI GGTGA 2 cut(s) 53, 118
Hpy166II GTNNAC 1 cut(s) 115
Hpy188III TCNNGA 3 cut(s) 59, 418, 449
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 316
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 3 cut(s) 41, 229, 302
HpyCH4V TGCA 3 cut(s) 132, 183, 376
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 1 cut(s) 439
LpnPI CCDG 6 cut(s) 44, 55, 82, 118, 142, 459
LweI GCATC 1 cut(s) 363
MaeIII GTNAC 1 cut(s) 223
MalI GATC 1 cut(s) 441
MboI GATC 1 cut(s) 439
MboII GAAGA 1 cut(s) 254
MfeI CAATTG 1 cut(s) 163
MhlI GDGCHC 2 cut(s) 24, 311
MlsI TGGCCA 2 cut(s) 73, 107
MluCI AATT 5 cut(s) 152, 163, 190, 380, 452
MluNI TGGCCA 2 cut(s) 73, 107
MlyI GAGTC 2 cut(s) 130, 152
MnlI CCTC 9 cut(s) 27, 37, 66, 75, 262, 270, 394, 397, 504
Mox20I TGGCCA 2 cut(s) 73, 107
MroXI GAANNNNTTC 1 cut(s) 521
MscI TGGCCA 2 cut(s) 73, 107
MseI TTAA 2 cut(s) 459, 510
Msp20I TGGCCA 2 cut(s) 73, 107
MspR9I CCNGG 1 cut(s) 70
MunI CAATTG 1 cut(s) 163
MvaI CCWGG 1 cut(s) 70
NdeII GATC 1 cut(s) 439
NlaIV GGNNCC 1 cut(s) 63
PaqCI CACCTGC 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 320
PdmI GAANNNNTTC 1 cut(s) 521
PfeI GAWTC 1 cut(s) 358
PflMI CCANNNNNTGG 1 cut(s) 395
PleI GAGTC 2 cut(s) 130, 152
PpsI GAGTC 2 cut(s) 130, 152
Psp6I CCWGG 1 cut(s) 68
PspGI CCWGG 1 cut(s) 68
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 1 cut(s) 61
PstI CTGCAG 1 cut(s) 134
RsaI GTAC 3 cut(s) 38, 149, 299
RsaNI GTAC 3 cut(s) 37, 148, 298
SalI GTCGAC 1 cut(s) 113
SaqAI TTAA 2 cut(s) 459, 510
Sau3AI GATC 1 cut(s) 439
Sau96I GGNCC 1 cut(s) 61
SbfI CCTGCAGG 1 cut(s) 134
SchI GAGTC 2 cut(s) 130, 152
ScrFI CCNGG 1 cut(s) 70
SdaI CCTGCAGG 1 cut(s) 134
SduI GDGCHC 2 cut(s) 24, 311
SetI ASST 9 cut(s) 38, 120, 131, 214, 331, 352, 408, 434, 496
SfaNI GCATC 1 cut(s) 363
SfcI CTRYAG 2 cut(s) 130, 542
SinI GGWCC 1 cut(s) 61
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse8387I CCTGCAGG 1 cut(s) 134
Sse9I AATT 5 cut(s) 152, 163, 190, 380, 452
StyD4I CCNGG 1 cut(s) 68
TaaI ACNGT 3 cut(s) 41, 229, 302
TaqI TCGA 3 cut(s) 114, 187, 311
TasI AATT 5 cut(s) 152, 163, 190, 380, 452
TatI WGTACW 1 cut(s) 297
TfiI GAWTC 1 cut(s) 358
Tru1I TTAA 2 cut(s) 459, 510
Tru9I TTAA 2 cut(s) 459, 510
TscAI CASTG 3 cut(s) 234, 346, 396
TspGWI ACGGA 3 cut(s) 7, 82, 129
TspRI CASTG 3 cut(s) 234, 346, 396
Van91I CCANNNNNTGG 1 cut(s) 395
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 2 cut(s) 190, 452
XmiI GTMKAC 1 cut(s) 114
XmnI GAANNNNTTC 1 cut(s) 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.