Rh2DG225900

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001616
Physical Location & Seq
Reverse (-)
22118484 .. 22120204
1721 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG225900.1

Sequence Viewer

Length: 606 bp
ATGTCACCGTTGATGGAATACATAGTCTCCCTGCTCTCGGTTTTGAGGTGCGATGAGGTGTTTTTGCCTCTGGACCCTTCTTGGCCAAAACAGAGGCTACTCTCCGTCATTGCTTTGGCCAATGTCAACCTAATAATCACCTGCAGGACTCCGTTTGGGTACAAATTGGACTCCAATTGGCTTGAGCAGAGTTACAATCAAAATTCTCTGTGGTTTTCCATAGGTGGAGAGTTTGTGATAGTGAGTGATGGAGGAGAGTGTAGGCGTGAGAGTGTGACAGAGGACGAGAGGCTGTTCTGTAATGTAATGTACAGTTCGGGCTCGACGGGGAAGCCGAAACGTGTTTGTGGCACTGAACAAGGTCTTTGGAATCGGTTTTTGTGGATGCAAGAATTGTATCCACTGAATGGAGATGAGGTTTTGTTGTTCAAGAGAGCAATAAGCTTTGTTGATCACTTTCAAGAATTTCTTAGCGCTATTCTTACTGGTTCTGCTTTGGTTATACCTCCTTTCAATCAACTTAAACAGAATGTGTTCTCCATTGTGGATTTCCTACAGTTTTGGAGGTGTAGTCTTGGCTTCTCGAGATGGGAAGATTGTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

201

Amino Acids

23.15

Weight (kDa)

4.96

Isoelectric Point (pI)

47.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 4 - 175 7.3e-16 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 149
AccB7I CCANNNNNTGG 1 cut(s) 407
AcoI YGGCCR 2 cut(s) 83, 117
AcsI RAATTY 2 cut(s) 202, 464
AfaI GTAC 2 cut(s) 161, 311
AfeI AGCGCT 1 cut(s) 475
AfiI CCNNNNNNNGG 2 cut(s) 37, 407
AflIII ACRYGT 1 cut(s) 340
AgsI TTSAA 3 cut(s) 430, 461, 514
AluBI AGCT 1 cut(s) 444
AluI AGCT 1 cut(s) 444
Alw26I GTCTC 1 cut(s) 31
Ama87I CYCGRG 1 cut(s) 583
Aor51HI AGCGCT 1 cut(s) 475
AoxI GGCC 2 cut(s) 83, 117
ApoI RAATTY 2 cut(s) 202, 464
Asp700I GAANNNNTTC 1 cut(s) 533
AspLEI GCGC 1 cut(s) 476
AspS9I GGNCC 1 cut(s) 73
AsuHPI GGTGA 1 cut(s) 130
AvaI CYCGRG 1 cut(s) 583
AvaII GGWCC 1 cut(s) 73
BalI TGGCCA 2 cut(s) 85, 119
BanII GRGCYC 1 cut(s) 323
BccI CCATC 3 cut(s) 7, 242, 582
BciVI GTATCC 1 cut(s) 408
BclI TGATCA 1 cut(s) 451
BcoDI GTCTC 1 cut(s) 31
BfmI CTRYAG 2 cut(s) 142, 554
BfoI RGCGCY 1 cut(s) 477
BfuAI ACCTGC 1 cut(s) 149
BfuI GTATCC 1 cut(s) 408
Bme18I GGWCC 1 cut(s) 73
BmeT110I CYCGRG 1 cut(s) 583
BmgT120I GGNCC 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 75
BmsI GCATC 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 203
BsaXI ACNNNNNCTCC 8 cut(s) 11, 41, 219, 249, 402, 432, 556, 586
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 407
Bse1I ACTGG 1 cut(s) 490
Bse3DI GCAATG 1 cut(s) 108
BseGI GGATG 1 cut(s) 390
BseLI CCNNNNNNNGG 2 cut(s) 37, 407
BseMI GCAATG 1 cut(s) 108
BseNI ACTGG 1 cut(s) 490
BseRI GAGGAG 1 cut(s) 267
BshFI GGCC 2 cut(s) 85, 119
BsiHKCI CYCGRG 1 cut(s) 583
BslI CCNNNNNNNGG 2 cut(s) 37, 407
BsmAI GTCTC 1 cut(s) 31
BsnI GGCC 2 cut(s) 85, 119
BsoBI CYCGRG 1 cut(s) 583
Bsp1286I GDGCHC 1 cut(s) 323
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 1 cut(s) 451
BspANI GGCC 2 cut(s) 85, 119
BspLI GGNNCC 1 cut(s) 75
BspMAI CTGCAG 1 cut(s) 146
BspMI ACCTGC 1 cut(s) 149
BsrDI GCAATG 1 cut(s) 108
BsrGI TGTACA 1 cut(s) 309
BsrI ACTGG 1 cut(s) 490
BssMI GATC 1 cut(s) 451
Bst4CI ACNGT 3 cut(s) 9, 314, 558
BstAUI TGTACA 1 cut(s) 309
BstDEI CTNAG 1 cut(s) 470
BstF5I GGATG 1 cut(s) 390
BstH2I RGCGCY 1 cut(s) 477
BstHHI GCGC 1 cut(s) 476
BstKTI GATC 1 cut(s) 454
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 1 cut(s) 451
BstSFI CTRYAG 2 cut(s) 142, 554
BsuI GTATCC 1 cut(s) 408
BsuRI GGCC 2 cut(s) 85, 119
BtgZI GCGATG 1 cut(s) 66
BtsCI GGATG 1 cut(s) 390
BtsIMutI CAGTG 2 cut(s) 351, 401
BveI ACCTGC 1 cut(s) 149
CfoI GCGC 1 cut(s) 476
Cfr13I GGNCC 1 cut(s) 73
Csp6I GTAC 2 cut(s) 160, 310
CviJI RGCY 9 cut(s) 85, 97, 119, 181, 292, 321, 334, 444, 579
CviKI_1 RGCY 9 cut(s) 85, 97, 119, 181, 292, 321, 334, 444, 579
CviQI GTAC 2 cut(s) 160, 310
DdeI CTNAG 1 cut(s) 470
DpnI GATC 1 cut(s) 453
DpnII GATC 1 cut(s) 451
EaeI YGGCCR 2 cut(s) 83, 117
Eco24I GRGCYC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 73
Eco47III AGCGCT 1 cut(s) 475
Eco88I CYCGRG 1 cut(s) 583
EcoT38I GRGCYC 1 cut(s) 323
FaiI YATR 3 cut(s) 23, 221, 503
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FbaI TGATCA 1 cut(s) 451
FokI GGATG 1 cut(s) 397
FriOI GRGCYC 1 cut(s) 323
GlaI GCGC 1 cut(s) 475
HaeII RGCGCY 1 cut(s) 477
HaeIII GGCC 2 cut(s) 85, 119
HhaI GCGC 1 cut(s) 476
Hin6I GCGC 1 cut(s) 474
HinP1I GCGC 1 cut(s) 474
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 442
HinfI GANTC 3 cut(s) 148, 170, 370
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 1 cut(s) 127
Hpy188III TCNNGA 5 cut(s) 71, 430, 461, 583, 585
Hpy8I GTNNAC 1 cut(s) 127
Hpy99I CGWCG 1 cut(s) 328
HpyAV CCTTC 1 cut(s) 87
HpyCH4III ACNGT 3 cut(s) 9, 314, 558
HpyCH4IV ACGT 1 cut(s) 340
HpyCH4V TGCA 2 cut(s) 144, 388
HpyF3I CTNAG 1 cut(s) 470
HpySE526I ACGT 1 cut(s) 340
HspAI GCGC 1 cut(s) 474
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 1 cut(s) 451
LpnPI CCDG 5 cut(s) 44, 56, 130, 154, 471
LweI GCATC 1 cut(s) 375
MaeII ACGT 1 cut(s) 340
MaeIII GTNAC 3 cut(s) 3, 191, 274
MalI GATC 1 cut(s) 453
MboI GATC 1 cut(s) 451
MboII GAAGA 1 cut(s) 605
MfeI CAATTG 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 323
MlsI TGGCCA 2 cut(s) 85, 119
MluCI AATT 5 cut(s) 164, 175, 202, 392, 464
MluNI TGGCCA 2 cut(s) 85, 119
MlyI GAGTC 2 cut(s) 142, 164
Mox20I TGGCCA 2 cut(s) 85, 119
MroXI GAANNNNTTC 1 cut(s) 533
MscI TGGCCA 2 cut(s) 85, 119
MseI TTAA 1 cut(s) 522
Msp20I TGGCCA 2 cut(s) 85, 119
MunI CAATTG 1 cut(s) 175
NdeII GATC 1 cut(s) 451
NlaIV GGNNCC 1 cut(s) 75
NmuCI GTSAC 2 cut(s) 3, 274
PaeR7I CTCGAG 1 cut(s) 583
PaqCI CACCTGC 1 cut(s) 149
PcsI WCGNNNNNNNCGW 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 533
PfeI GAWTC 1 cut(s) 370
PflMI CCANNNNNTGG 1 cut(s) 407
PleI GAGTC 2 cut(s) 142, 164
PpsI GAGTC 2 cut(s) 142, 164
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 1 cut(s) 73
PstI CTGCAG 1 cut(s) 146
RsaI GTAC 2 cut(s) 161, 311
RsaNI GTAC 2 cut(s) 160, 310
SaqAI TTAA 1 cut(s) 522
Sau3AI GATC 1 cut(s) 451
Sau96I GGNCC 1 cut(s) 73
SbfI CCTGCAGG 1 cut(s) 146
SchI GAGTC 2 cut(s) 142, 164
SdaI CCTGCAGG 1 cut(s) 146
SduI GDGCHC 1 cut(s) 323
SfaNI GCATC 1 cut(s) 375
SfcI CTRYAG 2 cut(s) 142, 554
Sfr274I CTCGAG 1 cut(s) 583
SinI GGWCC 1 cut(s) 73
SlaI CTCGAG 1 cut(s) 583
SmlI CTYRAG 2 cut(s) 182, 583
SmoI CTYRAG 2 cut(s) 182, 583
Sse8387I CCTGCAGG 1 cut(s) 146
Sse9I AATT 5 cut(s) 164, 175, 202, 392, 464
TaaI ACNGT 3 cut(s) 9, 314, 558
TaiI ACGT 1 cut(s) 343
TaqI TCGA 2 cut(s) 323, 584
TasI AATT 5 cut(s) 164, 175, 202, 392, 464
TatI WGTACW 1 cut(s) 309
TfiI GAWTC 1 cut(s) 370
Tru1I TTAA 1 cut(s) 522
Tru9I TTAA 1 cut(s) 522
TscAI CASTG 2 cut(s) 358, 408
TseFI GTSAC 2 cut(s) 3, 274
Tsp45I GTSAC 2 cut(s) 3, 274
TspGWI ACGGA 2 cut(s) 94, 141
TspRI CASTG 2 cut(s) 358, 408
Van91I CCANNNNNTGG 1 cut(s) 407
VpaK11BI GGWCC 1 cut(s) 73
XapI RAATTY 2 cut(s) 202, 464
XhoI CTCGAG 1 cut(s) 583
XmnI GAANNNNTTC 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.