Rh3BG369300

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0001616
Physical Location & Seq
Reverse (-)
46291826 .. 46294716
2891 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG369300.1

Sequence Viewer

Length: 504 bp
ATGCCACCTTTGATGGAATACATAGTCTCCGTGCTCTCGGTTTTGAGGTGCGGTGAGGCGTTTCTGCTTCTGGACCCTTCCTGGCCAAAACAGAGGCTACTCTTCGTGTGGTTTTCCATAGGTGGAGAGTTTGTGACAGTGAGTGATGGAGGAGAGTGTAGGCGTGAGAGTGAGAAAGAGGATGAGAGGCTGTTCTGTTATGTAATGTACAGTTCGGGCTCGACGGGGAAGCCGAAAGGTGTTTGTGGCACTGAACAAGGTCTTTGGAATCGGTTGCAAGAATTGTGTCCATTGAATGGAGAGGAGGTTTTGTTGTTCAAGACAACAATAAGCTTTGTTGATCAATTTCAAGAATTTCTTAGCGCTATTCTTACTGGTTCTGCTTTGGTTATACCTCCTTTCAATCAACTTAAACAGAATGTGTTCTCCATTGTGGATTTTCTACAGGTACATGACCTTCGCTTATTGTTTGTTTGGCTTTTCGTACTCAGATTATATTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

167

Amino Acids

19.1

Weight (kDa)

4.91

Isoelectric Point (pI)

40.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 56 - 150 4.8e-12 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 296
AciI CCGC 1 cut(s) 51
AcoI YGGCCR 1 cut(s) 83
AcsI RAATTY 1 cut(s) 353
AfaI GTAC 3 cut(s) 209, 450, 486
AfeI AGCGCT 1 cut(s) 364
AfiI CCNNNNNNNGG 1 cut(s) 296
AgsI TTSAA 4 cut(s) 295, 319, 350, 403
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 1 cut(s) 333
AluI AGCT 1 cut(s) 333
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 1 cut(s) 31
Aor51HI AGCGCT 1 cut(s) 364
AoxI GGCC 1 cut(s) 83
ApoI RAATTY 1 cut(s) 353
Asp700I GAANNNNTTC 1 cut(s) 422
AspLEI GCGC 1 cut(s) 365
AspS9I GGNCC 1 cut(s) 73
AsuHPI GGTGA 1 cut(s) 65
AvaII GGWCC 1 cut(s) 73
BalI TGGCCA 1 cut(s) 85
BanII GRGCYC 1 cut(s) 221
BarI GAAGNNNNNNTAC 2 cut(s) 441, 473
Bbv12I GWGCWC 1 cut(s) 36
BccI CCATC 2 cut(s) 7, 140
BciT130I CCWGG 1 cut(s) 82
BclI TGATCA 1 cut(s) 340
BcoDI GTCTC 1 cut(s) 31
BfmI CTRYAG 1 cut(s) 443
BfoI RGCGCY 1 cut(s) 366
Bme1390I CCNGG 1 cut(s) 82
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 75
BmrFI CCNGG 1 cut(s) 82
BsaXI ACNNNNNCTCC 8 cut(s) 11, 41, 117, 147, 291, 296, 321, 326
Bsc4I CCNNNNNNNGG 1 cut(s) 296
Bse1I ACTGG 1 cut(s) 379
BseBI CCWGG 1 cut(s) 82
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 296
BseMII CTCAG 1 cut(s) 502
BseNI ACTGG 1 cut(s) 379
BseRI GAGGAG 2 cut(s) 165, 317
BshFI GGCC 1 cut(s) 85
BsiHKAI GWGCWC 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 296
BsmAI GTCTC 1 cut(s) 31
BsnI GGCC 1 cut(s) 85
Bsp1286I GDGCHC 2 cut(s) 36, 221
Bsp1407I TGTACA 1 cut(s) 207
Bsp143I GATC 1 cut(s) 340
BspACI CCGC 1 cut(s) 51
BspANI GGCC 1 cut(s) 85
BspCNI CTCAG 1 cut(s) 501
BspLI GGNNCC 1 cut(s) 75
BsrGI TGTACA 1 cut(s) 207
BsrI ACTGG 1 cut(s) 379
BssMI GATC 1 cut(s) 340
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 2 cut(s) 139, 212
Bst6I CTCTTC 1 cut(s) 107
BstAUI TGTACA 1 cut(s) 207
BstDEI CTNAG 2 cut(s) 359, 488
BstF5I GGATG 1 cut(s) 187
BstH2I RGCGCY 1 cut(s) 366
BstHHI GCGC 1 cut(s) 365
BstKTI GATC 1 cut(s) 343
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 1 cut(s) 340
BstNI CCWGG 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 80
BstSFI CTRYAG 1 cut(s) 443
BsuRI GGCC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 187
BtsIMutI CAGTG 2 cut(s) 144, 249
CfoI GCGC 1 cut(s) 365
Cfr13I GGNCC 1 cut(s) 73
Csp6I GTAC 3 cut(s) 208, 449, 485
CviAII CATG 1 cut(s) 452
CviJI RGCY 7 cut(s) 85, 97, 190, 219, 232, 333, 478
CviKI_1 RGCY 7 cut(s) 85, 97, 190, 219, 232, 333, 478
CviQI GTAC 3 cut(s) 208, 449, 485
DdeI CTNAG 2 cut(s) 359, 488
DpnI GATC 1 cut(s) 342
DpnII GATC 1 cut(s) 340
EaeI YGGCCR 1 cut(s) 83
Eam1104I CTCTTC 1 cut(s) 107
EarI CTCTTC 1 cut(s) 107
Eco24I GRGCYC 1 cut(s) 221
Eco47I GGWCC 1 cut(s) 73
Eco47III AGCGCT 1 cut(s) 364
EcoRII CCWGG 1 cut(s) 80
EcoT38I GRGCYC 1 cut(s) 221
FaeI CATG 1 cut(s) 455
FaiI YATR 6 cut(s) 23, 119, 201, 392, 453, 496
FalI AAGNNNNNCTT 2 cut(s) 342, 374
FatI CATG 1 cut(s) 451
FbaI TGATCA 1 cut(s) 340
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 1 cut(s) 221
GlaI GCGC 1 cut(s) 364
HaeII RGCGCY 1 cut(s) 366
HaeIII GGCC 1 cut(s) 85
HhaI GCGC 1 cut(s) 365
Hin1II CATG 1 cut(s) 455
Hin6I GCGC 1 cut(s) 363
HinP1I GCGC 1 cut(s) 363
HindIII AAGCTT 1 cut(s) 331
HinfI GANTC 1 cut(s) 268
HphI GGTGA 1 cut(s) 65
Hpy188I TCNGA 1 cut(s) 491
Hpy188III TCNNGA 4 cut(s) 71, 319, 350, 501
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 2 cut(s) 87, 467
HpyCH4III ACNGT 2 cut(s) 139, 212
HpyCH4V TGCA 1 cut(s) 277
HpyF3I CTNAG 2 cut(s) 359, 488
Hsp92II CATG 1 cut(s) 455
HspAI GCGC 1 cut(s) 363
Ksp22I TGATCA 1 cut(s) 340
Kzo9I GATC 1 cut(s) 340
LpnPI CCDG 5 cut(s) 56, 67, 94, 360, 431
MaeIII GTNAC 1 cut(s) 133
MalI GATC 1 cut(s) 342
MboI GATC 1 cut(s) 340
MboII GAAGA 1 cut(s) 94
MhlI GDGCHC 2 cut(s) 36, 221
MlsI TGGCCA 1 cut(s) 85
MluCI AATT 3 cut(s) 281, 344, 353
MluNI TGGCCA 1 cut(s) 85
MnlI CCTC 9 cut(s) 39, 49, 87, 143, 172, 180, 295, 298, 405
Mox20I TGGCCA 1 cut(s) 85
MroXI GAANNNNTTC 1 cut(s) 422
MscI TGGCCA 1 cut(s) 85
MseI TTAA 1 cut(s) 411
Msp20I TGGCCA 1 cut(s) 85
MspR9I CCNGG 1 cut(s) 82
MvaI CCWGG 1 cut(s) 82
NdeII GATC 1 cut(s) 340
NlaIII CATG 1 cut(s) 455
NlaIV GGNNCC 1 cut(s) 75
NmuCI GTSAC 1 cut(s) 133
PcsI WCGNNNNNNNCGW 1 cut(s) 230
PdmI GAANNNNTTC 1 cut(s) 422
PfeI GAWTC 1 cut(s) 268
PflMI CCANNNNNTGG 1 cut(s) 296
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 1 cut(s) 73
RsaI GTAC 3 cut(s) 209, 450, 486
RsaNI GTAC 3 cut(s) 208, 449, 485
SaqAI TTAA 1 cut(s) 411
Sau3AI GATC 1 cut(s) 340
Sau96I GGNCC 1 cut(s) 73
ScrFI CCNGG 1 cut(s) 82
SduI GDGCHC 2 cut(s) 36, 221
SfcI CTRYAG 1 cut(s) 443
SinI GGWCC 1 cut(s) 73
Sse9I AATT 3 cut(s) 281, 344, 353
SsiI CCGC 1 cut(s) 51
StyD4I CCNGG 1 cut(s) 80
TaaI ACNGT 2 cut(s) 139, 212
TaqI TCGA 1 cut(s) 221
TasI AATT 3 cut(s) 281, 344, 353
TatI WGTACW 1 cut(s) 207
TfiI GAWTC 1 cut(s) 268
Tru1I TTAA 1 cut(s) 411
Tru9I TTAA 1 cut(s) 411
TscAI CASTG 2 cut(s) 144, 256
TseFI GTSAC 1 cut(s) 133
Tsp45I GTSAC 1 cut(s) 133
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 2 cut(s) 144, 256
Van91I CCANNNNNTGG 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 73
XapI RAATTY 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.