acyl-activating enzyme
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1994 |
| AccB1I | GGYRCC | 1 cut(s) | 1073 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1711 |
| AccI | GTMKAC | 1 cut(s) | 243 |
| AccII | CGCG | 1 cut(s) | 548 |
| AciI | CCGC | 7 cut(s) | 546, 662, 2621, 2735, 2795, 2882, 2936 |
| AclI | AACGTT | 1 cut(s) | 792 |
| AclWI | GGATC | 3 cut(s) | 120, 1284, 1943 |
| AcoI | YGGCCR | 2 cut(s) | 2108, 2121 |
| AcsI | RAATTY | 5 cut(s) | 55, 627, 929, 1388, 2259 |
| AcuI | CTGAAG | 3 cut(s) | 1475, 1593, 2761 |
| AcvI | CACGTG | 1 cut(s) | 1167 |
| AcyI | GRCGYC | 1 cut(s) | 50 |
| AfeI | AGCGCT | 3 cut(s) | 446, 1452, 2872 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 109, 382, 600, 893, 1021, 1186, 1711, 2184, 2287 |
| AflII | CTTAAG | 1 cut(s) | 848 |
| AflIII | ACRYGT | 1 cut(s) | 1164 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 170 |
| AjnI | CCWGG | 2 cut(s) | 599, 1586 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1075, 1107 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 794, 826 |
| Alw21I | GWGCWC | 3 cut(s) | 1626, 1968, 2528 |
| Alw26I | GTCTC | 4 cut(s) | 32, 41, 829, 2299 |
| Alw44I | GTGCAC | 1 cut(s) | 2524 |
| AlwI | GGATC | 3 cut(s) | 120, 1284, 1943 |
| Ama87I | CYCGRG | 2 cut(s) | 1403, 1506 |
| Aor51HI | AGCGCT | 3 cut(s) | 446, 1452, 2872 |
| AoxI | GGCC | 5 cut(s) | 324, 895, 1012, 2108, 2121 |
| ApaLI | GTGCAC | 1 cut(s) | 2524 |
| ApeKI | GCWGC | 4 cut(s) | 136, 1416, 2051, 2868 |
| ApoI | RAATTY | 5 cut(s) | 55, 627, 929, 1388, 2259 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2816, 2848 |
| AseI | ATTAAT | 1 cut(s) | 2760 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 2246, 2907 |
| AspA2I | CCTAGG | 1 cut(s) | 1999 |
| AspLEI | GCGC | 7 cut(s) | 447, 725, 906, 1057, 1453, 1796, 2873 |
| AspS9I | GGNCC | 5 cut(s) | 100, 325, 1013, 1565, 2440 |
| AsuC2I | CCSGG | 5 cut(s) | 666, 894, 2125, 2288, 2786 |
| AsuHPI | GGTGA | 5 cut(s) | 54, 1694, 1889, 2306, 2345 |
| AvaI | CYCGRG | 2 cut(s) | 1403, 1506 |
| AvaII | GGWCC | 3 cut(s) | 100, 1565, 2440 |
| AvrII | CCTAGG | 1 cut(s) | 1999 |
| BaeGI | GKGCMC | 3 cut(s) | 1287, 2528, 2707 |
| BanI | GGYRCC | 1 cut(s) | 1073 |
| BanII | GRGCYC | 4 cut(s) | 8, 593, 762, 1626 |
| BbrPI | CACGTG | 1 cut(s) | 1167 |
| Bbv12I | GWGCWC | 3 cut(s) | 1626, 1968, 2528 |
| BbvI | GCAGC | 4 cut(s) | 148, 1403, 2038, 2880 |
| BciT130I | CCWGG | 2 cut(s) | 601, 1588 |
| BciVI | GTATCC | 3 cut(s) | 562, 868, 2658 |
| BclI | TGATCA | 3 cut(s) | 1464, 2095, 2818 |
| BcnI | CCSGG | 5 cut(s) | 666, 894, 2125, 2288, 2786 |
| BcoDI | GTCTC | 4 cut(s) | 32, 41, 829, 2299 |
| BfaI | CTAG | 7 cut(s) | 233, 402, 468, 489, 1953, 2000, 2941 |
| BfmI | CTRYAG | 1 cut(s) | 2678 |
| BfoI | RGCGCY | 3 cut(s) | 448, 1454, 2874 |
| BfrI | CTTAAG | 1 cut(s) | 848 |
| BfuI | GTATCC | 3 cut(s) | 562, 868, 2658 |
| BisI | GCNGC | 5 cut(s) | 137, 546, 1417, 2052, 2869 |
| BlnI | CCTAGG | 1 cut(s) | 1999 |
| BlsI | GCNGC | 5 cut(s) | 138, 547, 1418, 2053, 2870 |
| BmcAI | AGTACT | 1 cut(s) | 871 |
| Bme1390I | CCNGG | 7 cut(s) | 601, 666, 894, 1588, 2125, 2288, 2786 |
| Bme18I | GGWCC | 3 cut(s) | 100, 1565, 2440 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 170 |
| BmeT110I | CYCGRG | 2 cut(s) | 1403, 1506 |
| BmgT120I | GGNCC | 5 cut(s) | 100, 325, 1013, 1565, 2440 |
| BmiI | GGNNCC | 7 cut(s) | 101, 590, 759, 1075, 1566, 2605, 2773 |
| BmrFI | CCNGG | 7 cut(s) | 601, 666, 894, 1588, 2125, 2288, 2786 |
| BmrI | ACTGGG | 2 cut(s) | 139, 2072 |
| BmsI | GCATC | 6 cut(s) | 532, 1504, 1745, 2212, 2752, 2869 |
| BmuI | ACTGGG | 2 cut(s) | 139, 2072 |
| BpmI | CTGGAG | 2 cut(s) | 531, 1982 |
| BpuEI | CTTGAG | 3 cut(s) | 628, 884, 1288 |
| BpuMI | CCSGG | 5 cut(s) | 666, 894, 2125, 2288, 2786 |
| BsaAI | YACGTR | 2 cut(s) | 1167, 2816 |
| BsaHI | GRCGYC | 1 cut(s) | 50 |
| BsaI | GGTCTC | 1 cut(s) | 32 |
| BsaJI | CCNNGG | 7 cut(s) | 762, 1016, 1411, 1999, 2124, 2178, 2287 |
| BsaWI | WCCGGW | 2 cut(s) | 75, 701 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 12, 42, 595, 625, 794, 824 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 109, 382, 600, 893, 1021, 1186, 1711, 2184, 2287 |
| Bse118I | RCCGGY | 1 cut(s) | 685 |
| Bse1I | ACTGG | 4 cut(s) | 134, 514, 614, 2078 |
| Bse3DI | GCAATG | 1 cut(s) | 376 |
| BseBI | CCWGG | 2 cut(s) | 601, 1588 |
| BseDI | CCNNGG | 7 cut(s) | 762, 1016, 1411, 1999, 2124, 2178, 2287 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 109, 382, 600, 893, 1021, 1186, 1711, 2184, 2287 |
| BseMI | GCAATG | 1 cut(s) | 376 |
| BseMII | CTCAG | 4 cut(s) | 1176, 1424, 2367, 2655 |
| BseNI | ACTGG | 4 cut(s) | 134, 514, 614, 2078 |
| BseRI | GAGGAG | 3 cut(s) | 815, 2163, 2644 |
| BseSI | GKGCMC | 3 cut(s) | 1287, 2528, 2707 |
| BseX3I | CGGCCG | 1 cut(s) | 2121 |
| BseXI | GCAGC | 4 cut(s) | 148, 1403, 2038, 2880 |
| Bsh1236I | CGCG | 1 cut(s) | 548 |
| Bsh1285I | CGRYCG | 3 cut(s) | 287, 693, 2124 |
| BshFI | GGCC | 5 cut(s) | 326, 897, 1014, 2110, 2123 |
| BshNI | GGYRCC | 1 cut(s) | 1073 |
| BsiEI | CGRYCG | 3 cut(s) | 287, 693, 2124 |
| BsiHKAI | GWGCWC | 3 cut(s) | 1626, 1968, 2528 |
| BsiHKCI | CYCGRG | 2 cut(s) | 1403, 1506 |
| BslFI | GGGAC | 4 cut(s) | 158, 644, 2024, 2577 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 109, 382, 600, 893, 1021, 1186, 1711, 2184, 2287 |
| BsmAI | GTCTC | 4 cut(s) | 32, 41, 829, 2299 |
| BsmBI | CGTCTC | 1 cut(s) | 41 |
| BsmFI | GGGAC | 4 cut(s) | 158, 644, 2024, 2577 |
| BsnI | GGCC | 5 cut(s) | 326, 897, 1014, 2110, 2123 |
| Bso31I | GGTCTC | 1 cut(s) | 32 |
| BsoBI | CYCGRG | 2 cut(s) | 1403, 1506 |
| Bsp1286I | GDGCHC | 8 cut(s) | 8, 593, 762, 1287, 1626, 1968, 2528, 2707 |
| Bsp1407I | TGTACA | 3 cut(s) | 1097, 1161, 1498 |
| Bsp19I | CCATGG | 2 cut(s) | 762, 1411 |
| BspACI | CCGC | 7 cut(s) | 546, 662, 2621, 2735, 2795, 2882, 2936 |
| BspANI | GGCC | 5 cut(s) | 326, 897, 1014, 2110, 2123 |
| BspCNI | CTCAG | 4 cut(s) | 1177, 1425, 2368, 2654 |
| BspFNI | CGCG | 1 cut(s) | 548 |
| BspHI | TCATGA | 1 cut(s) | 2821 |
| BspLI | GGNNCC | 7 cut(s) | 101, 590, 759, 1075, 1566, 2605, 2773 |
| BspPI | GGATC | 3 cut(s) | 120, 1284, 1943 |
| BspT107I | GGYRCC | 1 cut(s) | 1073 |
| BspTI | CTTAAG | 1 cut(s) | 848 |
| BspTNI | GGTCTC | 1 cut(s) | 32 |
| BsrDI | GCAATG | 1 cut(s) | 376 |
| BsrFI | RCCGGY | 1 cut(s) | 685 |
| BsrGI | TGTACA | 3 cut(s) | 1097, 1161, 1498 |
| BsrI | ACTGG | 4 cut(s) | 134, 514, 614, 2078 |
| BssAI | RCCGGY | 1 cut(s) | 685 |
| BssECI | CCNNGG | 7 cut(s) | 762, 1016, 1411, 1999, 2124, 2178, 2287 |
| BssNI | GRCGYC | 1 cut(s) | 50 |
| BssT1I | CCWWGG | 4 cut(s) | 762, 1411, 1999, 2178 |
| Bst2UI | CCWGG | 2 cut(s) | 601, 1588 |
| Bst4CI | ACNGT | 9 cut(s) | 199, 559, 1043, 1123, 1554, 2520, 2568, 2682, 2828 |
| Bst6I | CTCTTC | 1 cut(s) | 2600 |
| BstACI | GRCGYC | 1 cut(s) | 50 |
| BstAFI | CTTAAG | 1 cut(s) | 848 |
| BstAUI | TGTACA | 3 cut(s) | 1097, 1161, 1498 |
| BstBAI | YACGTR | 2 cut(s) | 1167, 2816 |
| BstC8I | GCNNGC | 4 cut(s) | 1519, 1523, 1527, 2841 |
| BstDSI | CCRYGG | 3 cut(s) | 762, 1016, 1411 |
| BstFNI | CGCG | 1 cut(s) | 548 |
| BstH2I | RGCGCY | 3 cut(s) | 448, 1454, 2874 |
| BstHHI | GCGC | 7 cut(s) | 447, 725, 906, 1057, 1453, 1796, 2873 |
| BstMAI | GTCTC | 4 cut(s) | 32, 41, 829, 2299 |
| BstMCI | CGRYCG | 3 cut(s) | 287, 693, 2124 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 142, 451, 903, 2462, 2627, 2718, 2879 |
| BstNI | CCWGG | 2 cut(s) | 601, 1588 |
| BstNSI | RCATGY | 2 cut(s) | 1505, 2583 |
| BstSCI | CCNGG | 7 cut(s) | 599, 664, 892, 1586, 2123, 2286, 2784 |
| BstSFI | CTRYAG | 1 cut(s) | 2678 |
| BstSLI | GKGCMC | 3 cut(s) | 1287, 2528, 2707 |
| BstUI | CGCG | 1 cut(s) | 548 |
| BstV1I | GCAGC | 4 cut(s) | 148, 1403, 2038, 2880 |
| BstX2I | RGATCY | 1 cut(s) | 112 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1010 |
| BstYI | RGATCY | 1 cut(s) | 112 |
| BstZI | CGGCCG | 1 cut(s) | 2121 |
| BsuI | GTATCC | 3 cut(s) | 562, 868, 2658 |
| BsuRI | GGCC | 5 cut(s) | 326, 897, 1014, 2110, 2123 |
| BtgI | CCRYGG | 3 cut(s) | 762, 1016, 1411 |
| BtsI | GCAGTG | 2 cut(s) | 774, 2738 |
| BtsIMutI | CAGTG | 6 cut(s) | 405, 774, 1990, 2649, 2738, 2866 |
| Cac8I | GCNNGC | 4 cut(s) | 1519, 1523, 1527, 2841 |
| CciI | TCATGA | 1 cut(s) | 2821 |
| CfoI | GCGC | 7 cut(s) | 447, 725, 906, 1057, 1453, 1796, 2873 |
| Cfr10I | RCCGGY | 1 cut(s) | 685 |
| Cfr13I | GGNCC | 5 cut(s) | 100, 325, 1013, 1565, 2440 |
| CseI | GACGC | 3 cut(s) | 23, 58, 2043 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1331, 1366 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1994 |
| DriI | GACNNNNNGTC | 1 cut(s) | 170 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1994 |
| EaeI | YGGCCR | 2 cut(s) | 2108, 2121 |
| EagI | CGGCCG | 1 cut(s) | 2121 |
| Eam1104I | CTCTTC | 1 cut(s) | 2600 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 170 |
| EarI | CTCTTC | 1 cut(s) | 2600 |
| EciI | GGCGGA | 1 cut(s) | 2724 |
| Ecl136II | GAGCTC | 1 cut(s) | 1624 |
| EclXI | CGGCCG | 1 cut(s) | 2121 |
| Eco130I | CCWWGG | 4 cut(s) | 762, 1411, 1999, 2178 |
| Eco24I | GRGCYC | 4 cut(s) | 8, 593, 762, 1626 |
| Eco31I | GGTCTC | 1 cut(s) | 32 |
| Eco47I | GGWCC | 3 cut(s) | 100, 1565, 2440 |
| Eco47III | AGCGCT | 3 cut(s) | 446, 1452, 2872 |
| Eco52I | CGGCCG | 1 cut(s) | 2121 |
| Eco53kI | GAGCTC | 1 cut(s) | 1624 |
| Eco57I | CTGAAG | 3 cut(s) | 1475, 1593, 2761 |
| Eco72I | CACGTG | 1 cut(s) | 1167 |
| Eco88I | CYCGRG | 2 cut(s) | 1403, 1506 |
| EcoICRI | GAGCTC | 1 cut(s) | 1624 |
| EcoO109I | RGGNCCY | 2 cut(s) | 325, 1565 |
| EcoRII | CCWGG | 2 cut(s) | 599, 1586 |
| EcoT14I | CCWWGG | 4 cut(s) | 762, 1411, 1999, 2178 |
| EcoT38I | GRGCYC | 4 cut(s) | 8, 593, 762, 1626 |
| ErhI | CCWWGG | 4 cut(s) | 762, 1411, 1999, 2178 |
| Esp3I | CGTCTC | 1 cut(s) | 41 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 1075, 1107, 1997, 2029, 2579, 2611 |
| FaqI | GGGAC | 4 cut(s) | 158, 644, 2024, 2577 |
| FauI | CCCGC | 3 cut(s) | 669, 2614, 2788 |
| FauNDI | CATATG | 2 cut(s) | 1113, 2400 |
| FbaI | TGATCA | 3 cut(s) | 1464, 2095, 2818 |
| FblI | GTMKAC | 1 cut(s) | 243 |
| Fnu4HI | GCNGC | 5 cut(s) | 137, 546, 1417, 2052, 2869 |
| FriOI | GRGCYC | 4 cut(s) | 8, 593, 762, 1626 |
| Fsp4HI | GCNGC | 5 cut(s) | 137, 546, 1417, 2052, 2869 |
| FspBI | CTAG | 7 cut(s) | 233, 402, 468, 489, 1953, 2000, 2941 |
| GlaI | GCGC | 7 cut(s) | 446, 724, 905, 1056, 1452, 1795, 2872 |
| GluI | GCNGC | 5 cut(s) | 137, 546, 1417, 2052, 2869 |
| GsuI | CTGGAG | 2 cut(s) | 531, 1982 |
| HaeII | RGCGCY | 3 cut(s) | 448, 1454, 2874 |
| HaeIII | GGCC | 5 cut(s) | 326, 897, 1014, 2110, 2123 |
| HgaI | GACGC | 3 cut(s) | 23, 58, 2043 |
| HhaI | GCGC | 7 cut(s) | 447, 725, 906, 1057, 1453, 1796, 2873 |
| Hin1I | GRCGYC | 1 cut(s) | 50 |
| Hin6I | GCGC | 7 cut(s) | 445, 723, 904, 1055, 1451, 1794, 2871 |
| HinP1I | GCGC | 7 cut(s) | 445, 723, 904, 1055, 1451, 1794, 2871 |
| HincII | GTYRAC | 3 cut(s) | 187, 244, 2133 |
| HindII | GTYRAC | 3 cut(s) | 187, 244, 2133 |
| HindIII | AAGCTT | 3 cut(s) | 1859, 1935, 2145 |
| HphI | GGTGA | 5 cut(s) | 54, 1694, 1889, 2306, 2345 |
| Hpy188III | TCNNGA | 6 cut(s) | 1403, 1532, 1961, 2822, 2900, 2927 |
| Hpy99I | CGWCG | 4 cut(s) | 429, 462, 486, 2122 |
| HpyAV | CCTTC | 7 cut(s) | 1342, 1578, 1585, 1859, 1897, 2453, 2785 |
| HpyCH4III | ACNGT | 9 cut(s) | 199, 559, 1043, 1123, 1554, 2520, 2568, 2682, 2828 |
| HpyCH4IV | ACGT | 7 cut(s) | 792, 1005, 1166, 1321, 2135, 2548, 2815 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 142, 451, 903, 2462, 2627, 2718, 2879 |
| HpySE526I | ACGT | 7 cut(s) | 792, 1005, 1166, 1321, 2135, 2548, 2815 |
| Hsp92I | GRCGYC | 1 cut(s) | 50 |
| HspAI | GCGC | 7 cut(s) | 445, 723, 904, 1055, 1451, 1794, 2871 |
| Ksp22I | TGATCA | 3 cut(s) | 1464, 2095, 2818 |
| LmnI | GCTCC | 8 cut(s) | 133, 588, 603, 757, 849, 1240, 1963, 2609 |
| Lsp1109I | GCAGC | 4 cut(s) | 148, 1403, 2038, 2880 |
| LweI | GCATC | 6 cut(s) | 532, 1504, 1745, 2212, 2752, 2869 |
| MaeI | CTAG | 7 cut(s) | 233, 402, 468, 489, 1953, 2000, 2941 |
| MaeII | ACGT | 7 cut(s) | 792, 1005, 1166, 1321, 2135, 2548, 2815 |
| MboII | GAAGA | 6 cut(s) | 636, 972, 975, 1586, 1803, 2587 |
| MfeI | CAATTG | 2 cut(s) | 951, 2690 |
| MflI | RGATCY | 1 cut(s) | 112 |
| MhlI | GDGCHC | 8 cut(s) | 8, 593, 762, 1287, 1626, 1968, 2528, 2707 |
| MlyI | GAGTC | 6 cut(s) | 619, 2236, 2301, 2353, 2376, 2725 |
| MmeI | TCCRAC | 2 cut(s) | 646, 2305 |
| MroXI | GAANNNNTTC | 2 cut(s) | 2246, 2907 |
| MseI | TTAA | 8 cut(s) | 849, 1383, 1674, 1829, 1941, 2138, 2411, 2760 |
| MslI | CAYNNNNRTG | 6 cut(s) | 540, 767, 1008, 2578, 2720, 2826 |
| MspA1I | CMGCKG | 1 cut(s) | 2621 |
| MspCI | CTTAAG | 1 cut(s) | 848 |
| MspR9I | CCNGG | 7 cut(s) | 601, 666, 894, 1588, 2125, 2288, 2786 |
| MunI | CAATTG | 2 cut(s) | 951, 2690 |
| MvaI | CCWGG | 2 cut(s) | 601, 1588 |
| MvnI | CGCG | 1 cut(s) | 548 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 142, 451, 903, 2462, 2627, 2718, 2879 |
| NciI | CCSGG | 5 cut(s) | 666, 894, 2125, 2288, 2786 |
| NcoI | CCATGG | 2 cut(s) | 762, 1411 |
| NdeI | CATATG | 2 cut(s) | 1113, 2400 |
| NlaIV | GGNNCC | 7 cut(s) | 101, 590, 759, 1075, 1566, 2605, 2773 |
| NmuCI | GTSAC | 6 cut(s) | 277, 697, 1985, 2055, 2312, 2685 |
| NspI | RCATGY | 2 cut(s) | 1505, 2583 |
| PaeR7I | CTCGAG | 1 cut(s) | 1403 |
| PagI | TCATGA | 1 cut(s) | 2821 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 430 |
| PdmI | GAANNNNTTC | 2 cut(s) | 2246, 2907 |
| PfeI | GAWTC | 6 cut(s) | 397, 1174, 1782, 2114, 2488, 2615 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1711 |
| PfoI | TCCNGGA | 1 cut(s) | 599 |
| PkrI | GCNGC | 5 cut(s) | 138, 547, 1418, 2053, 2870 |
| Ple19I | CGATCG | 1 cut(s) | 287 |
| PleI | GAGTC | 6 cut(s) | 618, 2235, 2300, 2352, 2375, 2725 |
| PmaCI | CACGTG | 1 cut(s) | 1167 |
| PmlI | CACGTG | 1 cut(s) | 1167 |
| PpsI | GAGTC | 6 cut(s) | 618, 2235, 2300, 2352, 2375, 2725 |
| Ppu21I | YACGTR | 2 cut(s) | 1167, 2816 |
| PpuMI | RGGWCCY | 1 cut(s) | 1565 |
| PshBI | ATTAAT | 1 cut(s) | 2760 |
| Psp124BI | GAGCTC | 1 cut(s) | 1626 |
| Psp1406I | AACGTT | 1 cut(s) | 792 |
| Psp5II | RGGWCCY | 1 cut(s) | 1565 |
| Psp6I | CCWGG | 2 cut(s) | 599, 1586 |
| PspCI | CACGTG | 1 cut(s) | 1167 |
| PspGI | CCWGG | 2 cut(s) | 599, 1586 |
| PspN4I | GGNNCC | 7 cut(s) | 101, 590, 759, 1075, 1566, 2605, 2773 |
| PspPI | GGNCC | 5 cut(s) | 100, 325, 1013, 1565, 2440 |
| PspPPI | RGGWCCY | 1 cut(s) | 1565 |
| PsuI | RGATCY | 1 cut(s) | 112 |
| PvuI | CGATCG | 1 cut(s) | 287 |
| RseI | CAYNNNNRTG | 6 cut(s) | 540, 767, 1008, 2578, 2720, 2826 |
| SacI | GAGCTC | 1 cut(s) | 1626 |
| SalI | GTCGAC | 1 cut(s) | 242 |
| SaqAI | TTAA | 8 cut(s) | 849, 1383, 1674, 1829, 1941, 2138, 2411, 2760 |
| SatI | GCNGC | 5 cut(s) | 137, 546, 1417, 2052, 2869 |
| Sau96I | GGNCC | 5 cut(s) | 100, 325, 1013, 1565, 2440 |
| ScaI | AGTACT | 1 cut(s) | 871 |
| SchI | GAGTC | 6 cut(s) | 619, 2236, 2301, 2353, 2376, 2725 |
| ScrFI | CCNGG | 7 cut(s) | 601, 666, 894, 1588, 2125, 2288, 2786 |
| SduI | GDGCHC | 8 cut(s) | 8, 593, 762, 1287, 1626, 1968, 2528, 2707 |
| SfaNI | GCATC | 6 cut(s) | 532, 1504, 1745, 2212, 2752, 2869 |
| SfcI | CTRYAG | 1 cut(s) | 2678 |
| Sfr274I | CTCGAG | 1 cut(s) | 1403 |
| SinI | GGWCC | 3 cut(s) | 100, 1565, 2440 |
| SlaI | CTCGAG | 1 cut(s) | 1403 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 540, 767, 1008, 2578, 2720, 2826 |
| SmlI | CTYRAG | 5 cut(s) | 607, 848, 899, 1303, 1403 |
| SmoI | CTYRAG | 5 cut(s) | 607, 848, 899, 1303, 1403 |
| SsiI | CCGC | 7 cut(s) | 546, 662, 2621, 2735, 2795, 2882, 2936 |
| SspI | AATATT | 2 cut(s) | 1945, 2889 |
| SspMI | CTAG | 7 cut(s) | 233, 402, 468, 489, 1953, 2000, 2941 |
| SstI | GAGCTC | 1 cut(s) | 1626 |
| StyD4I | CCNGG | 7 cut(s) | 599, 664, 892, 1586, 2123, 2286, 2784 |
| StyI | CCWWGG | 4 cut(s) | 762, 1411, 1999, 2178 |
| TaaI | ACNGT | 9 cut(s) | 199, 559, 1043, 1123, 1554, 2520, 2568, 2682, 2828 |
| TaiI | ACGT | 7 cut(s) | 795, 1008, 1169, 1324, 2138, 2551, 2818 |
| TaqI | TCGA | 6 cut(s) | 193, 243, 287, 449, 1404, 2365 |
| TaqII | GACCGA | 1 cut(s) | 707 |
| TatI | WGTACW | 6 cut(s) | 869, 1045, 1097, 1161, 1498, 2071 |
| TauI | GCSGC | 1 cut(s) | 548 |
| TfiI | GAWTC | 6 cut(s) | 397, 1174, 1782, 2114, 2488, 2615 |
| Tru1I | TTAA | 8 cut(s) | 849, 1383, 1674, 1829, 1941, 2138, 2411, 2760 |
| Tru9I | TTAA | 8 cut(s) | 849, 1383, 1674, 1829, 1941, 2138, 2411, 2760 |
| TscAI | CASTG | 6 cut(s) | 412, 774, 1990, 2649, 2745, 2866 |
| TseFI | GTSAC | 6 cut(s) | 277, 697, 1985, 2055, 2312, 2685 |
| TseI | GCWGC | 4 cut(s) | 136, 1416, 2051, 2868 |
| Tsp45I | GTSAC | 6 cut(s) | 277, 697, 1985, 2055, 2312, 2685 |
| TspDTI | ATGAA | 8 cut(s) | 922, 998, 1302, 1981, 2387, 2550, 2704, 2900 |
| TspGWI | ACGGA | 1 cut(s) | 1804 |
| TspRI | CASTG | 6 cut(s) | 412, 774, 1990, 2649, 2745, 2866 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1711 |
| Vha464I | CTTAAG | 1 cut(s) | 848 |
| VneI | GTGCAC | 1 cut(s) | 2524 |
| VpaK11BI | GGWCC | 3 cut(s) | 100, 1565, 2440 |
| VspI | ATTAAT | 1 cut(s) | 2760 |
| XapI | RAATTY | 5 cut(s) | 55, 627, 929, 1388, 2259 |
| XceI | RCATGY | 2 cut(s) | 1505, 2583 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 232 |
| XhoI | CTCGAG | 1 cut(s) | 1403 |
| XmaJI | CCTAGG | 1 cut(s) | 1999 |
| XmiI | GTMKAC | 1 cut(s) | 243 |
| XmnI | GAANNNNTTC | 2 cut(s) | 2246, 2907 |
| XspI | CTAG | 7 cut(s) | 233, 402, 468, 489, 1953, 2000, 2941 |
| ZrmI | AGTACT | 1 cut(s) | 871 |