Rh4AG016000

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001616
Physical Location & Seq
Forward (+)
3202422 .. 3211884
9463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG016000.1

Sequence Viewer

Length: 447 bp
ATGCCGCCATCGGTGGAATACATAGTCTCCATGCTCTCGGTTTTGAGGTGCAGCGAGGCGTTTCTGCCTCTGGACCAGTCCTGGCCAAAACAGAGGCTACTCTCCGTCGTTGCTTCGGCTAATGTCGACCTAATTATCACTTGCACGACTCCGTTTGGTTACGAATTGGACTCCAATTGGCTGGAAAAGAATATCAATCTTAGCTTTCTGTGGTTTTCCATGGGTGGTGGAGAGTCTGTGACAGTGAGTGATGGAGAGCAGCGTAAGAGTGAGAAAGAGAGGCTGTTCTGTTATGTAATGTACACTTCGTGCTCGACAGGGAAGCCGAAAGGTGTCTGTGGAACTGAACAAGGTCTTTGGAATCGGTTTCGGTGGATGCAAGAATTGTATCCATTGAATGGAGAGGAGCATGTATCAAATATTCTGTACAATGATTGCATGGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

148

Amino Acids

16.84

Weight (kDa)

4.86

Isoelectric Point (pI)

54.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 1 - 127 6.1e-15 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 398
AccI GTMKAC 1 cut(s) 126
AciI CCGC 1 cut(s) 5
AcoI YGGCCR 1 cut(s) 83
AdeI CACNNNGTG 1 cut(s) 309
AfaI GTAC 2 cut(s) 302, 428
AfiI CCNNNNNNNGG 1 cut(s) 398
AgsI TTSAA 1 cut(s) 397
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 1 cut(s) 204
AluI AGCT 1 cut(s) 204
Alw21I GWGCWC 1 cut(s) 314
Alw26I GTCTC 1 cut(s) 31
AoxI GGCC 1 cut(s) 83
ApeKI GCWGC 2 cut(s) 51, 259
AspS9I GGNCC 1 cut(s) 73
AvaII GGWCC 1 cut(s) 73
BalI TGGCCA 1 cut(s) 85
Bbv12I GWGCWC 1 cut(s) 314
BbvI GCAGC 2 cut(s) 63, 271
BccI CCATC 2 cut(s) 16, 245
BciT130I CCWGG 1 cut(s) 82
BciVI GTATCC 1 cut(s) 399
BcoDI GTCTC 1 cut(s) 31
BfuI GTATCC 1 cut(s) 399
BisI GCNGC 3 cut(s) 5, 52, 260
BlsI GCNGC 3 cut(s) 6, 53, 261
Bme1390I CCNGG 1 cut(s) 82
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 73
BmrFI CCNGG 1 cut(s) 82
BmsI GCATC 1 cut(s) 366
BsaJI CCNNGG 1 cut(s) 219
BsaXI ACNNNNNCTCC 4 cut(s) 11, 41, 222, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 398
Bse1I ACTGG 1 cut(s) 76
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 1 cut(s) 381
BseLI CCNNNNNNNGG 1 cut(s) 398
BseNI ACTGG 1 cut(s) 76
BseRI GAGGAG 1 cut(s) 419
BseXI GCAGC 2 cut(s) 63, 271
BsgI GTGCAG 1 cut(s) 70
BshFI GGCC 1 cut(s) 85
BsiHKAI GWGCWC 1 cut(s) 314
BslI CCNNNNNNNGG 1 cut(s) 398
BsmAI GTCTC 1 cut(s) 31
BsnI GGCC 1 cut(s) 85
Bsp1286I GDGCHC 1 cut(s) 314
Bsp1407I TGTACA 2 cut(s) 300, 426
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 85
BsrGI TGTACA 2 cut(s) 300, 426
BsrI ACTGG 1 cut(s) 76
BssECI CCNNGG 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 82
Bst4CI ACNGT 1 cut(s) 244
BstAUI TGTACA 2 cut(s) 300, 426
BstDEI CTNAG 1 cut(s) 200
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 1 cut(s) 381
BstMAI GTCTC 1 cut(s) 31
BstNI CCWGG 1 cut(s) 82
BstNSI RCATGY 1 cut(s) 413
BstSCI CCNGG 1 cut(s) 80
BstV1I GCAGC 2 cut(s) 63, 271
BstXI CCANNNNNNTGG 1 cut(s) 181
BsuI GTATCC 1 cut(s) 399
BsuRI GGCC 1 cut(s) 85
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 1 cut(s) 381
BtsIMutI CAGTG 1 cut(s) 249
Cfr13I GGNCC 1 cut(s) 73
Csp6I GTAC 2 cut(s) 301, 427
CviAII CATG 4 cut(s) 31, 220, 410, 439
CviJI RGCY 7 cut(s) 85, 97, 119, 181, 204, 283, 325
CviKI_1 RGCY 7 cut(s) 85, 97, 119, 181, 204, 283, 325
CviQI GTAC 2 cut(s) 301, 427
DdeI CTNAG 1 cut(s) 200
DraIII CACNNNGTG 1 cut(s) 309
EaeI YGGCCR 1 cut(s) 83
Eco130I CCWWGG 1 cut(s) 219
Eco47I GGWCC 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 4 cut(s) 34, 223, 413, 442
FaiI YATR 7 cut(s) 23, 32, 221, 294, 411, 440, 445
FatI CATG 4 cut(s) 30, 219, 409, 438
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 3 cut(s) 5, 52, 260
FokI GGATG 1 cut(s) 388
Fsp4HI GCNGC 3 cut(s) 5, 52, 260
GluI GCNGC 3 cut(s) 5, 52, 260
HaeIII GGCC 1 cut(s) 85
Hin1II CATG 4 cut(s) 34, 223, 413, 442
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 4 cut(s) 148, 170, 233, 361
Hpy166II GTNNAC 2 cut(s) 127, 303
Hpy188III TCNNGA 1 cut(s) 71
Hpy8I GTNNAC 2 cut(s) 127, 303
Hpy99I CGWCG 1 cut(s) 110
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 4 cut(s) 51, 144, 379, 438
HpyF3I CTNAG 1 cut(s) 200
Hsp92II CATG 4 cut(s) 34, 223, 413, 442
LmnI GCTCC 1 cut(s) 406
LpnPI CCDG 6 cut(s) 56, 67, 89, 94, 167, 303
Lsp1109I GCAGC 2 cut(s) 63, 271
LweI GCATC 1 cut(s) 366
MaeIII GTNAC 2 cut(s) 158, 238
MfeI CAATTG 1 cut(s) 175
MhlI GDGCHC 1 cut(s) 314
MlsI TGGCCA 1 cut(s) 85
MluCI AATT 4 cut(s) 132, 164, 175, 383
MluNI TGGCCA 1 cut(s) 85
MlyI GAGTC 3 cut(s) 142, 164, 242
MnlI CCTC 6 cut(s) 39, 49, 78, 87, 273, 397
Mox20I TGGCCA 1 cut(s) 85
MscI TGGCCA 1 cut(s) 85
Msp20I TGGCCA 1 cut(s) 85
MspR9I CCNGG 1 cut(s) 82
MunI CAATTG 1 cut(s) 175
MvaI CCWGG 1 cut(s) 82
NcoI CCATGG 1 cut(s) 219
NlaIII CATG 4 cut(s) 34, 223, 413, 442
NmuCI GTSAC 1 cut(s) 238
NspI RCATGY 1 cut(s) 413
PfeI GAWTC 1 cut(s) 361
PflMI CCANNNNNTGG 1 cut(s) 398
PkrI GCNGC 3 cut(s) 6, 53, 261
PleI GAGTC 3 cut(s) 142, 164, 241
PpsI GAGTC 3 cut(s) 142, 164, 241
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspPI GGNCC 1 cut(s) 73
RsaI GTAC 2 cut(s) 302, 428
RsaNI GTAC 2 cut(s) 301, 427
SalI GTCGAC 1 cut(s) 125
SatI GCNGC 3 cut(s) 5, 52, 260
Sau96I GGNCC 1 cut(s) 73
SchI GAGTC 3 cut(s) 142, 164, 242
ScrFI CCNGG 1 cut(s) 82
SduI GDGCHC 1 cut(s) 314
SetI ASST 5 cut(s) 50, 132, 206, 334, 355
SfaNI GCATC 1 cut(s) 366
SinI GGWCC 1 cut(s) 73
Sse9I AATT 4 cut(s) 132, 164, 175, 383
SsiI CCGC 1 cut(s) 5
SspI AATATT 1 cut(s) 421
StyD4I CCNGG 1 cut(s) 80
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 1 cut(s) 244
TaqI TCGA 2 cut(s) 126, 314
TasI AATT 4 cut(s) 132, 164, 175, 383
TatI WGTACW 2 cut(s) 300, 426
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 1 cut(s) 361
TscAI CASTG 1 cut(s) 249
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 2 cut(s) 51, 259
Tsp45I GTSAC 1 cut(s) 238
TspGWI ACGGA 2 cut(s) 94, 141
TspRI CASTG 1 cut(s) 249
Van91I CCANNNNNTGG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 73
XceI RCATGY 1 cut(s) 413
XmiI GTMKAC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.