Rh1BG120900

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001616
Physical Location & Seq
Reverse (-)
20530746 .. 20532436
1691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG120900.1

Sequence Viewer

Length: 834 bp
ATGAGAAGCCATGAAGGTTCTATATTCCACCACTTCTCCAAAGCAGCGTCCCAAAACCTCAACAAAATCGCAGTCATACACGCCTCCGGTGACCGCCGTACTCGCACCTCCATCACCGTCCAACCTCTCTACGACGGCGACCGCTTCTTCACGTACGCAGAGTTAATCTCCGCCGTCGATACCCTCACTTCGCAGCTCCGTCGCAATAACCTTAATTCAGCTTCATCAGAGCTCCGACCGAGAATTCTCGGCATATATATGCCGCCGTCGGTGGAATACATAGTCTCCGTGCTCTCGGTTTTGAGGTGCGGCGAGGCGTTTCTGCCTCTGGACCCGTCCTGGCCAAAACAGAGGCTGCTCTCCGTCGTTGCTTCGGCCAATGTCGACCTAATTATCACCTGCACGACGCCGTTTGGATACGAATTGGACTCCAATTGGCTCAAGCAGAGTTGCAATTGTAGCTTTCTGTGGTTTTCCATGGGTGGAGAGTCTGTGACAGTGAGTGATGGAGGAGAGTGTAAGAGTGAGAAAGAGAGGCTGTTCTGTTATGTAATGTACACTTCGGGCTCGACGGGGAAGCCGAAAGGTGTCTGCGGAACTGAACAAGGTCTTTGGAATCGGTTTCGGTGGATGCAAGAATTGCATCCATTGAATGGAGAGGAGGTTTTGTTGTTCAAGACAGCAATAAGCTTTGTTGATCACTTGCAAGAATTTCTTAGTGCTATTCTTACTGGTTCTACTTTGGTCATACCTCCTTTCAACCAGCTTAAACAGAATGTGTTCTCCATTGTTGATTTTCTACAGTTACATGACCCTTGCTTATTGTTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

277

Amino Acids

31.1

Weight (kDa)

6.38

Isoelectric Point (pI)

46.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 27 - 258 9.5e-28 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 407
Acc36I ACCTGC 1 cut(s) 407
AccB7I CCANNNNNTGG 1 cut(s) 653
AccI GTMKAC 1 cut(s) 384
AciI CCGC 6 cut(s) 94, 142, 171, 263, 309, 594
AcoI YGGCCR 2 cut(s) 341, 375
AcsI RAATTY 2 cut(s) 243, 710
AcyI GRCGYC 1 cut(s) 407
AfaI GTAC 3 cut(s) 100, 155, 557
AfiI CCNNNNNNNGG 1 cut(s) 653
AgsI TTSAA 3 cut(s) 652, 676, 760
AjnI CCWGG 1 cut(s) 338
AluBI AGCT 6 cut(s) 196, 221, 232, 462, 690, 766
AluI AGCT 6 cut(s) 196, 221, 232, 462, 690, 766
Alw21I GWGCWC 2 cut(s) 234, 294
Alw26I GTCTC 1 cut(s) 289
AlwNI CAGNNNCTG 1 cut(s) 355
AoxI GGCC 2 cut(s) 341, 375
ApeKI GCWGC 3 cut(s) 44, 193, 355
ApoI RAATTY 2 cut(s) 243, 710
ArsI GACNNNNNNTTYG 2 cut(s) 57, 89
Asp700I GAANNNNTTC 1 cut(s) 779
AspS9I GGNCC 1 cut(s) 331
AsuHPI GGTGA 3 cut(s) 101, 106, 388
AvaII GGWCC 1 cut(s) 331
BalI TGGCCA 1 cut(s) 343
BanII GRGCYC 2 cut(s) 234, 569
Bbv12I GWGCWC 2 cut(s) 234, 294
BbvI GCAGC 3 cut(s) 56, 205, 342
BccI CCATC 2 cut(s) 119, 500
BceAI ACGGC 5 cut(s) 81, 151, 158, 250, 394
BcgI CGANNNNNNTGC 2 cut(s) 182, 216
BciT130I CCWGG 1 cut(s) 340
BciVI GTATCC 1 cut(s) 410
BclI TGATCA 1 cut(s) 697
BcoDI GTCTC 1 cut(s) 289
BfmI CTRYAG 1 cut(s) 800
BfuAI ACCTGC 1 cut(s) 407
BfuI GTATCC 1 cut(s) 410
BisI GCNGC 5 cut(s) 45, 194, 263, 310, 356
BlsI GCNGC 5 cut(s) 46, 195, 264, 311, 357
Bme1390I CCNGG 1 cut(s) 340
Bme18I GGWCC 1 cut(s) 331
BmgT120I GGNCC 1 cut(s) 331
BmiI GGNNCC 1 cut(s) 333
BmrFI CCNGG 1 cut(s) 340
BmsI GCATC 2 cut(s) 621, 652
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BpuEI CTTGAG 1 cut(s) 425
BsaAI YACGTR 1 cut(s) 153
BsaHI GRCGYC 1 cut(s) 407
BsaJI CCNNGG 1 cut(s) 477
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 1 cut(s) 653
Bse1I ACTGG 1 cut(s) 736
BseBI CCWGG 1 cut(s) 340
BseDI CCNNGG 1 cut(s) 477
BseGI GGATG 2 cut(s) 636, 643
BseLI CCNNNNNNNGG 1 cut(s) 653
BseNI ACTGG 1 cut(s) 736
BseRI GAGGAG 2 cut(s) 525, 674
BseXI GCAGC 3 cut(s) 56, 205, 342
BsgI GTGCAG 1 cut(s) 385
Bsh1285I CGRYCG 2 cut(s) 142, 239
BshFI GGCC 2 cut(s) 343, 377
BsiEI CGRYCG 2 cut(s) 142, 239
BsiHKAI GWGCWC 2 cut(s) 234, 294
BsiSI CCGG 1 cut(s) 87
BsiWI CGTACG 1 cut(s) 153
BslFI GGGAC 1 cut(s) 34
BslI CCNNNNNNNGG 1 cut(s) 653
BsmAI GTCTC 1 cut(s) 289
BsmFI GGGAC 1 cut(s) 34
BsnI GGCC 2 cut(s) 343, 377
Bsp1286I GDGCHC 3 cut(s) 234, 294, 569
Bsp1407I TGTACA 1 cut(s) 555
Bsp143I GATC 1 cut(s) 697
Bsp19I CCATGG 1 cut(s) 477
BspACI CCGC 6 cut(s) 94, 142, 171, 263, 309, 594
BspANI GGCC 2 cut(s) 343, 377
BspLI GGNNCC 1 cut(s) 333
BspMI ACCTGC 1 cut(s) 407
BsrGI TGTACA 1 cut(s) 555
BsrI ACTGG 1 cut(s) 736
BssECI CCNNGG 1 cut(s) 477
BssMI GATC 1 cut(s) 697
BssNI GRCGYC 1 cut(s) 407
BssT1I CCWWGG 1 cut(s) 477
Bst2UI CCWGG 1 cut(s) 340
Bst4CI ACNGT 3 cut(s) 118, 499, 804
BstACI GRCGYC 1 cut(s) 407
BstAPI GCANNNNNTGC 1 cut(s) 640
BstAUI TGTACA 1 cut(s) 555
BstBAI YACGTR 1 cut(s) 153
BstDEI CTNAG 1 cut(s) 716
BstDSI CCRYGG 1 cut(s) 477
BstEII GGTNACC 1 cut(s) 89
BstF5I GGATG 2 cut(s) 636, 643
BstKTI GATC 1 cut(s) 700
BstMAI GTCTC 1 cut(s) 289
BstMBI GATC 1 cut(s) 697
BstMCI CGRYCG 2 cut(s) 142, 239
BstMWI GCNNNNNNNGC 3 cut(s) 102, 459, 640
BstNI CCWGG 1 cut(s) 340
BstPI GGTNACC 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 338
BstSFI CTRYAG 1 cut(s) 800
BstV1I GCAGC 3 cut(s) 56, 205, 342
BsuI GTATCC 1 cut(s) 410
BsuRI GGCC 2 cut(s) 343, 377
BtgI CCRYGG 1 cut(s) 477
BtsCI GGATG 2 cut(s) 636, 643
BtsIMutI CAGTG 1 cut(s) 504
BveI ACCTGC 1 cut(s) 407
CaiI CAGNNNCTG 1 cut(s) 355
Cfr13I GGNCC 1 cut(s) 331
CseI GACGC 2 cut(s) 36, 415
Csp6I GTAC 3 cut(s) 99, 154, 556
CviAII CATG 3 cut(s) 11, 478, 809
CviQI GTAC 3 cut(s) 99, 154, 556
DdeI CTNAG 1 cut(s) 716
DpnI GATC 1 cut(s) 699
DpnII GATC 1 cut(s) 697
EaeI YGGCCR 2 cut(s) 341, 375
EciI GGCGGA 1 cut(s) 160
Ecl136II GAGCTC 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 477
Eco24I GRGCYC 2 cut(s) 234, 569
Eco47I GGWCC 1 cut(s) 331
Eco53kI GAGCTC 1 cut(s) 232
Eco91I GGTNACC 1 cut(s) 89
EcoICRI GAGCTC 1 cut(s) 232
EcoO65I GGTNACC 1 cut(s) 89
EcoRI GAATTC 1 cut(s) 243
EcoRII CCWGG 1 cut(s) 338
EcoT14I CCWWGG 1 cut(s) 477
EcoT38I GRGCYC 2 cut(s) 234, 569
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 3 cut(s) 14, 481, 812
FalI AAGNNNNNCTT 2 cut(s) 699, 731
FaqI GGGAC 1 cut(s) 34
FatI CATG 3 cut(s) 10, 477, 808
FbaI TGATCA 1 cut(s) 697
FblI GTMKAC 1 cut(s) 384
Fnu4HI GCNGC 5 cut(s) 45, 194, 263, 310, 356
FokI GGATG 2 cut(s) 630, 643
FriOI GRGCYC 2 cut(s) 234, 569
Fsp4HI GCNGC 5 cut(s) 45, 194, 263, 310, 356
GluI GCNGC 5 cut(s) 45, 194, 263, 310, 356
HaeIII GGCC 2 cut(s) 343, 377
HapII CCGG 1 cut(s) 87
HgaI GACGC 2 cut(s) 36, 415
Hin1I GRCGYC 1 cut(s) 407
Hin1II CATG 3 cut(s) 14, 481, 812
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HindIII AAGCTT 1 cut(s) 688
HinfI GANTC 3 cut(s) 428, 488, 616
HpaII CCGG 1 cut(s) 87
HphI GGTGA 3 cut(s) 101, 106, 388
Hpy166II GTNNAC 2 cut(s) 385, 558
Hpy188I TCNGA 2 cut(s) 229, 236
Hpy188III TCNNGA 2 cut(s) 329, 676
Hpy8I GTNNAC 2 cut(s) 385, 558
Hpy99I CGWCG 7 cut(s) 137, 179, 204, 271, 368, 409, 574
HpyAV CCTTC 1 cut(s) 8
HpyCH4III ACNGT 3 cut(s) 118, 499, 804
HpyCH4IV ACGT 1 cut(s) 152
HpyCH4V TGCA 5 cut(s) 402, 453, 634, 643, 706
HpyF10VI GCNNNNNNNGC 3 cut(s) 102, 459, 640
HpyF3I CTNAG 1 cut(s) 716
HpySE526I ACGT 1 cut(s) 152
Hsp92I GRCGYC 1 cut(s) 407
Hsp92II CATG 3 cut(s) 14, 481, 812
Ksp22I TGATCA 1 cut(s) 697
Kzo9I GATC 1 cut(s) 697
LmnI GCTCC 2 cut(s) 201, 237
LpnPI CCDG 7 cut(s) 100, 314, 325, 352, 412, 717, 776
Lsp1109I GCAGC 3 cut(s) 56, 205, 342
LweI GCATC 2 cut(s) 621, 652
MaeII ACGT 1 cut(s) 152
MaeIII GTNAC 3 cut(s) 89, 493, 804
MalI GATC 1 cut(s) 699
MboI GATC 1 cut(s) 697
MboII GAAGA 1 cut(s) 139
MfeI CAATTG 2 cut(s) 433, 454
MhlI GDGCHC 3 cut(s) 234, 294, 569
MlsI TGGCCA 1 cut(s) 343
MluCI AATT 8 cut(s) 214, 243, 390, 422, 433, 454, 638, 710
MluNI TGGCCA 1 cut(s) 343
MlyI GAGTC 2 cut(s) 422, 497
MmeI TCCRAC 2 cut(s) 145, 259
Mox20I TGGCCA 1 cut(s) 343
MroXI GAANNNNTTC 1 cut(s) 779
MscI TGGCCA 1 cut(s) 343
MseI TTAA 3 cut(s) 164, 213, 768
MslI CAYNNNNRTG 1 cut(s) 257
Msp20I TGGCCA 1 cut(s) 343
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 1 cut(s) 340
MunI CAATTG 2 cut(s) 433, 454
MvaI CCWGG 1 cut(s) 340
MwoI GCNNNNNNNGC 3 cut(s) 102, 459, 640
NcoI CCATGG 1 cut(s) 477
NdeII GATC 1 cut(s) 697
NlaIII CATG 3 cut(s) 14, 481, 812
NlaIV GGNNCC 1 cut(s) 333
NmeAIII GCCGAG 1 cut(s) 228
NmuCI GTSAC 2 cut(s) 89, 493
PaqCI CACCTGC 1 cut(s) 407
PcsI WCGNNNNNNNCGW 1 cut(s) 578
PdmI GAANNNNTTC 1 cut(s) 779
PfeI GAWTC 1 cut(s) 616
Pfl23II CGTACG 1 cut(s) 153
PflMI CCANNNNNTGG 1 cut(s) 653
PkrI GCNGC 5 cut(s) 46, 195, 264, 311, 357
PleI GAGTC 2 cut(s) 422, 496
PpsI GAGTC 2 cut(s) 422, 496
Ppu21I YACGTR 1 cut(s) 153
Psp124BI GAGCTC 1 cut(s) 234
Psp6I CCWGG 1 cut(s) 338
PspEI GGTNACC 1 cut(s) 89
PspGI CCWGG 1 cut(s) 338
PspLI CGTACG 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 333
PspPI GGNCC 1 cut(s) 331
PstNI CAGNNNCTG 1 cut(s) 355
RsaI GTAC 3 cut(s) 100, 155, 557
RsaNI GTAC 3 cut(s) 99, 154, 556
RseI CAYNNNNRTG 1 cut(s) 257
SacI GAGCTC 1 cut(s) 234
SalI GTCGAC 1 cut(s) 383
SaqAI TTAA 3 cut(s) 164, 213, 768
SatI GCNGC 5 cut(s) 45, 194, 263, 310, 356
Sau3AI GATC 1 cut(s) 697
Sau96I GGNCC 1 cut(s) 331
SchI GAGTC 2 cut(s) 422, 497
ScrFI CCNGG 1 cut(s) 340
SduI GDGCHC 3 cut(s) 234, 294, 569
SfaNI GCATC 2 cut(s) 621, 652
SfcI CTRYAG 1 cut(s) 800
SinI GGWCC 1 cut(s) 331
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 1 cut(s) 440
SmoI CTYRAG 1 cut(s) 440
Sse9I AATT 8 cut(s) 214, 243, 390, 422, 433, 454, 638, 710
SsiI CCGC 6 cut(s) 94, 142, 171, 263, 309, 594
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 1 cut(s) 338
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 3 cut(s) 118, 499, 804
TaiI ACGT 1 cut(s) 155
TaqI TCGA 3 cut(s) 177, 384, 569
TaqII GACCGA 1 cut(s) 253
TasI AATT 8 cut(s) 214, 243, 390, 422, 433, 454, 638, 710
TatI WGTACW 1 cut(s) 555
TauI GCSGC 2 cut(s) 265, 312
TfiI GAWTC 1 cut(s) 616
Tru1I TTAA 3 cut(s) 164, 213, 768
Tru9I TTAA 3 cut(s) 164, 213, 768
TscAI CASTG 1 cut(s) 504
TseFI GTSAC 2 cut(s) 89, 493
TseI GCWGC 3 cut(s) 44, 193, 355
Tsp45I GTSAC 2 cut(s) 89, 493
TspDTI ATGAA 2 cut(s) 27, 213
TspGWI ACGGA 3 cut(s) 188, 277, 352
TspRI CASTG 1 cut(s) 504
Van91I CCANNNNNTGG 1 cut(s) 653
VpaK11BI GGWCC 1 cut(s) 331
XapI RAATTY 2 cut(s) 243, 710
XmiI GTMKAC 1 cut(s) 384
XmnI GAANNNNTTC 1 cut(s) 779
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.