Rroxscaffold_1G00024170

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001616
Physical Location & Seq
Forward (+)
30049562 .. 30051346
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00024170.1

Sequence Viewer

Length: 537 bp
ATGCAAGAACTGTATCCACTGAATGGAGAGGAGGTTTTGTTGTTCAAGACAGCAATAAGCTTTGTTGATCACTTTCAAGAATTTCTTAGCCCTATTCTTACCGGTTCTGCTTTGGTTATACCTCCTTTCAATCAACTTAAACAGAATGTGTTCTCCATTGTGGATTTTCTACAGTTCTCTCTCTCCCTACGCTACTCCCCTCCAGCACTCTCCGCCTCTCCATCGACCCACTCGCCGTCTCTAAAGTCGATGTCGTTTTGCACCGATCTTTATCCGGCCGGAGCCGAGTCGCCGGTGACCACCGGACCTCCATCCGCGACTAGAAAGCTACCTAGAGACGAAATGAAAAGCAATGACAATCATGAAGACGGTCGGAGCTGCGGTCGAGACTCGATCTGTGCCTACAACTTCTTCTTCCTCATCGCAGATTTCGTTCTCATTTCCCGCTCCCAAAACCCTAACCATAACGCCGCATCGGCTTTTCCAGTTGTCGGTGAGTCCTCTCAATTTTCAATTTCGGACATATTTTTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

178

Amino Acids

19.62

Weight (kDa)

5.05

Isoelectric Point (pI)

53.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 23
AccBSI CCGCTC 1 cut(s) 447
AccII CGCG 1 cut(s) 317
AciI CCGC 5 cut(s) 213, 315, 381, 445, 471
AcoI YGGCCR 1 cut(s) 276
AcsI RAATTY 1 cut(s) 80
AfiI CCNNNNNNNGG 2 cut(s) 23, 491
AgeI ACCGGT 1 cut(s) 101
AgsI TTSAA 4 cut(s) 46, 77, 130, 513
AluBI AGCT 3 cut(s) 60, 328, 378
AluI AGCT 3 cut(s) 60, 328, 378
Alw26I GTCTC 3 cut(s) 243, 330, 381
AoxI GGCC 1 cut(s) 276
ApeKI GCWGC 1 cut(s) 378
ApoI RAATTY 1 cut(s) 80
AsiGI ACCGGT 1 cut(s) 101
Asp700I GAANNNNTTC 1 cut(s) 149
AspS9I GGNCC 1 cut(s) 305
AsuHPI GGTGA 2 cut(s) 307, 506
AvaII GGWCC 1 cut(s) 305
BbsI GAAGAC 1 cut(s) 372
BbvI GCAGC 1 cut(s) 365
BccI CCATC 2 cut(s) 229, 319
BceAI ACGGC 1 cut(s) 220
BciVI GTATCC 1 cut(s) 24
BclI TGATCA 1 cut(s) 67
BcoDI GTCTC 3 cut(s) 243, 330, 381
BfaI CTAG 2 cut(s) 321, 333
BfmI CTRYAG 1 cut(s) 170
BfuI GTATCC 1 cut(s) 24
BglI GCCNNNNNGGC 1 cut(s) 476
BisI GCNGC 2 cut(s) 379, 471
BlsI GCNGC 2 cut(s) 380, 472
Bme18I GGWCC 1 cut(s) 305
BmgT120I GGNCC 1 cut(s) 305
BmiI GGNNCC 1 cut(s) 283
BmsI GCATC 1 cut(s) 482
BpiI GAAGAC 1 cut(s) 372
BpmI CTGGAG 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 270
BsaWI WCCGGW 2 cut(s) 101, 302
BsaXI ACNNNNNCTCC 8 cut(s) 18, 23, 48, 53, 292, 322, 367, 397
Bsc4I CCNNNNNNNGG 2 cut(s) 23, 491
Bse118I RCCGGY 2 cut(s) 101, 292
Bse1I ACTGG 1 cut(s) 485
Bse3DI GCAATG 1 cut(s) 358
Bse8I GATNNNNATC 1 cut(s) 270
BseGI GGATG 1 cut(s) 311
BseJI GATNNNNATC 1 cut(s) 270
BseLI CCNNNNNNNGG 2 cut(s) 23, 491
BseMI GCAATG 1 cut(s) 358
BseNI ACTGG 1 cut(s) 485
BseRI GAGGAG 1 cut(s) 44
BseX3I CGGCCG 1 cut(s) 276
BseXI GCAGC 1 cut(s) 365
Bsh1236I CGCG 1 cut(s) 317
Bsh1285I CGRYCG 3 cut(s) 279, 373, 385
BshFI GGCC 1 cut(s) 278
BshTI ACCGGT 1 cut(s) 101
BsiEI CGRYCG 3 cut(s) 279, 373, 385
BsiSI CCGG 5 cut(s) 102, 275, 279, 293, 303
BslI CCNNNNNNNGG 2 cut(s) 23, 491
BsmAI GTCTC 3 cut(s) 243, 330, 381
BsmBI CGTCTC 2 cut(s) 243, 330
BsnI GGCC 1 cut(s) 278
Bsp143I GATC 3 cut(s) 67, 265, 393
BspACI CCGC 5 cut(s) 213, 315, 381, 445, 471
BspANI GGCC 1 cut(s) 278
BspFNI CGCG 1 cut(s) 317
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 283
BsrBI CCGCTC 1 cut(s) 447
BsrDI GCAATG 1 cut(s) 358
BsrFI RCCGGY 2 cut(s) 101, 292
BsrI ACTGG 1 cut(s) 485
BssAI RCCGGY 2 cut(s) 101, 292
BssMI GATC 3 cut(s) 67, 265, 393
Bst4CI ACNGT 3 cut(s) 12, 174, 371
BstDEI CTNAG 1 cut(s) 86
BstEII GGTNACC 1 cut(s) 295
BstF5I GGATG 1 cut(s) 311
BstFNI CGCG 1 cut(s) 317
BstKTI GATC 3 cut(s) 70, 268, 396
BstMAI GTCTC 3 cut(s) 243, 330, 381
BstMBI GATC 3 cut(s) 67, 265, 393
BstMCI CGRYCG 3 cut(s) 279, 373, 385
BstMWI GCNNNNNNNGC 2 cut(s) 212, 476
BstPI GGTNACC 1 cut(s) 295
BstSFI CTRYAG 1 cut(s) 170
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 1 cut(s) 365
BstV2I GAAGAC 1 cut(s) 372
BstZI CGGCCG 1 cut(s) 276
BsuI GTATCC 1 cut(s) 24
BsuRI GGCC 1 cut(s) 278
BtgZI GCGATG 1 cut(s) 406
BtsCI GGATG 1 cut(s) 311
BtsIMutI CAGTG 1 cut(s) 17
CciI TCATGA 1 cut(s) 361
Cfr10I RCCGGY 2 cut(s) 101, 292
Cfr13I GGNCC 1 cut(s) 305
CspAI ACCGGT 1 cut(s) 101
CviAII CATG 1 cut(s) 362
CviJI RGCY 7 cut(s) 60, 90, 278, 284, 328, 378, 479
CviKI_1 RGCY 7 cut(s) 60, 90, 278, 284, 328, 378, 479
DdeI CTNAG 1 cut(s) 86
DpnI GATC 3 cut(s) 69, 267, 395
DpnII GATC 3 cut(s) 67, 265, 393
EaeI YGGCCR 1 cut(s) 276
EagI CGGCCG 1 cut(s) 276
EciI GGCGGA 1 cut(s) 202
EclXI CGGCCG 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 305
Eco52I CGGCCG 1 cut(s) 276
Eco91I GGTNACC 1 cut(s) 295
EcoO65I GGTNACC 1 cut(s) 295
Esp3I CGTCTC 2 cut(s) 243, 330
FaeI CATG 1 cut(s) 365
FaiI YATR 4 cut(s) 119, 363, 465, 524
FalI AAGNNNNNCTT 2 cut(s) 69, 101
FatI CATG 1 cut(s) 361
FauI CCCGC 1 cut(s) 452
FbaI TGATCA 1 cut(s) 67
Fnu4HI GCNGC 2 cut(s) 379, 471
FokI GGATG 1 cut(s) 298
Fsp4HI GCNGC 2 cut(s) 379, 471
FspBI CTAG 2 cut(s) 321, 333
GluI GCNGC 2 cut(s) 379, 471
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 1 cut(s) 278
HapII CCGG 5 cut(s) 102, 275, 279, 293, 303
Hin1II CATG 1 cut(s) 365
HindIII AAGCTT 1 cut(s) 58
HinfI GANTC 3 cut(s) 287, 389, 497
HpaII CCGG 5 cut(s) 102, 275, 279, 293, 303
HphI GGTGA 2 cut(s) 307, 506
Hpy188I TCNGA 2 cut(s) 375, 520
Hpy188III TCNNGA 4 cut(s) 46, 77, 362, 386
HpyCH4III ACNGT 3 cut(s) 12, 174, 371
HpyCH4V TGCA 2 cut(s) 4, 261
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 476
HpyF3I CTNAG 1 cut(s) 86
Hsp92II CATG 1 cut(s) 365
Ksp22I TGATCA 1 cut(s) 67
Kzo9I GATC 3 cut(s) 67, 265, 393
LmnI GCTCC 3 cut(s) 281, 375, 452
LpnPI CCDG 7 cut(s) 115, 216, 288, 292, 306, 316, 498
Lsp1109I GCAGC 1 cut(s) 365
LweI GCATC 1 cut(s) 482
MaeI CTAG 2 cut(s) 321, 333
MaeIII GTNAC 1 cut(s) 295
MalI GATC 3 cut(s) 69, 267, 395
MbiI CCGCTC 1 cut(s) 447
MboI GATC 3 cut(s) 67, 265, 393
MboII GAAGA 3 cut(s) 377, 403, 406
MluCI AATT 3 cut(s) 80, 506, 513
MlyI GAGTC 3 cut(s) 296, 383, 506
MmeI TCCRAC 1 cut(s) 353
MnlI CCTC 8 cut(s) 22, 25, 132, 210, 226, 318, 428, 511
MroXI GAANNNNTTC 1 cut(s) 149
MseI TTAA 2 cut(s) 138, 535
MspI CCGG 5 cut(s) 102, 275, 279, 293, 303
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 2 cut(s) 212, 476
NdeII GATC 3 cut(s) 67, 265, 393
NlaIII CATG 1 cut(s) 365
NlaIV GGNNCC 1 cut(s) 283
NmeAIII GCCGAG 1 cut(s) 310
NmuCI GTSAC 1 cut(s) 295
PagI TCATGA 1 cut(s) 361
PcsI WCGNNNNNNNCGW 1 cut(s) 429
PdmI GAANNNNTTC 1 cut(s) 149
PflMI CCANNNNNTGG 1 cut(s) 23
PinAI ACCGGT 1 cut(s) 101
PkrI GCNGC 2 cut(s) 380, 472
PleI GAGTC 3 cut(s) 295, 383, 505
PpsI GAGTC 3 cut(s) 295, 383, 505
PspEI GGTNACC 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 283
PspPI GGNCC 1 cut(s) 305
SaqAI TTAA 2 cut(s) 138, 535
SatI GCNGC 2 cut(s) 379, 471
Sau3AI GATC 3 cut(s) 67, 265, 393
Sau96I GGNCC 1 cut(s) 305
SchI GAGTC 3 cut(s) 296, 383, 506
SetI ASST 7 cut(s) 36, 62, 124, 310, 330, 334, 380
SfaNI GCATC 1 cut(s) 482
SfcI CTRYAG 1 cut(s) 170
SgrAI CRCCGGYG 1 cut(s) 292
SinI GGWCC 1 cut(s) 305
Sse9I AATT 3 cut(s) 80, 506, 513
SsiI CCGC 5 cut(s) 213, 315, 381, 445, 471
SspMI CTAG 2 cut(s) 321, 333
TaaI ACNGT 3 cut(s) 12, 174, 371
TaqI TCGA 4 cut(s) 224, 248, 385, 392
TasI AATT 3 cut(s) 80, 506, 513
TauI GCSGC 1 cut(s) 473
Tru1I TTAA 2 cut(s) 138, 535
Tru9I TTAA 2 cut(s) 138, 535
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 1 cut(s) 378
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 2 cut(s) 359, 378
TspRI CASTG 1 cut(s) 24
Van91I CCANNNNNTGG 1 cut(s) 23
VpaK11BI GGWCC 1 cut(s) 305
XapI RAATTY 1 cut(s) 80
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 2 cut(s) 321, 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.