RLG00000021608

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001616
Physical Location & Seq
Forward (+)
76705233 .. 76705739
507 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021608

Sequence Viewer

Length: 507 bp
ATGGAATACATAGTCTCCGTGCTCTCGGTTTTGAGGTGCGGTGAGGCTTTTCTGCCTTTGGACCCTTCCTGGCCAAAACAGAGGCTATTCTCCGTCATTGCTTTGGCCAATGTCGACGTAATAATCACCTGCAGGACTCCGTTTGGGTACAAATTGGACTCCAATTGGCTCGAGCAGAGTTGCAATCGAAATTCTCTGTGGTTTTCCATAGGTGGAGAGTTTGTGACAGTGAGTGATGGAGAAAAGTGTAGGCGTGAGAGTGAGAAAGAGGATGAGAGGCTGTTCTGTTATGTAATGTACAGTTCGGGCTCGATGGGGAAGCCGAAAGGTGTTTGTGGCACTGAACAAGGTCTTTGGAATCGGTTTCTGTGGATGCAAGAATTGTATCCACTGAATGGAGAGGAGGTTTTGTTGTTCAAGACAGCAATAAGCTTTGTTGATCACTTTCAAGAATTTCTTAACGCGATTCTTACTGGTTCTGCTTTGGTTATACCTCCTTTCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

169

Amino Acids

19.15

Weight (kDa)

4.95

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 2 - 166 6.5e-17 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 395
AccI GTMKAC 1 cut(s) 114
AccII CGCG 1 cut(s) 464
AciI CCGC 1 cut(s) 39
AcoI YGGCCR 2 cut(s) 71, 105
AcsI RAATTY 2 cut(s) 190, 452
AfaI GTAC 2 cut(s) 149, 299
AfiI CCNNNNNNNGG 1 cut(s) 395
AgsI TTSAA 3 cut(s) 418, 449, 502
AjnI CCWGG 1 cut(s) 68
AluBI AGCT 1 cut(s) 432
AluI AGCT 1 cut(s) 432
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 2 cut(s) 71, 105
ApoI RAATTY 2 cut(s) 190, 452
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 2 cut(s) 53, 118
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 1 cut(s) 61
BalI TGGCCA 2 cut(s) 73, 107
BanII GRGCYC 1 cut(s) 311
Bbv12I GWGCWC 1 cut(s) 24
BccI CCATC 2 cut(s) 230, 307
BciT130I CCWGG 1 cut(s) 70
BciVI GTATCC 1 cut(s) 396
BclI TGATCA 1 cut(s) 439
BcoDI GTCTC 1 cut(s) 19
BfmI CTRYAG 1 cut(s) 130
BfuAI ACCTGC 1 cut(s) 137
BfuI GTATCC 1 cut(s) 396
Bme1390I CCNGG 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 70
BmsI GCATC 1 cut(s) 363
BsaXI ACNNNNNCTCC 7 cut(s) 29, 207, 237, 390, 395, 420, 425
Bsc4I CCNNNNNNNGG 1 cut(s) 395
Bse1I ACTGG 1 cut(s) 478
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 70
BseGI GGATG 2 cut(s) 277, 378
BseLI CCNNNNNNNGG 1 cut(s) 395
BseMI GCAATG 1 cut(s) 96
BseNI ACTGG 1 cut(s) 478
BseRI GAGGAG 1 cut(s) 416
Bsh1236I CGCG 1 cut(s) 464
BshFI GGCC 2 cut(s) 73, 107
BsiHKAI GWGCWC 1 cut(s) 24
BsiHKCI CYCGRG 1 cut(s) 170
BslI CCNNNNNNNGG 1 cut(s) 395
BsmAI GTCTC 1 cut(s) 19
BsnI GGCC 2 cut(s) 73, 107
BsoBI CYCGRG 1 cut(s) 170
Bsp1286I GDGCHC 2 cut(s) 24, 311
Bsp1407I TGTACA 1 cut(s) 297
Bsp143I GATC 1 cut(s) 439
BspACI CCGC 1 cut(s) 39
BspANI GGCC 2 cut(s) 73, 107
BspFNI CGCG 1 cut(s) 464
BspLI GGNNCC 1 cut(s) 63
BspMAI CTGCAG 1 cut(s) 134
BspMI ACCTGC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 96
BsrGI TGTACA 1 cut(s) 297
BsrI ACTGG 1 cut(s) 478
BssMI GATC 1 cut(s) 439
Bst2UI CCWGG 1 cut(s) 70
Bst4CI ACNGT 2 cut(s) 229, 302
BstAUI TGTACA 1 cut(s) 297
BstF5I GGATG 2 cut(s) 277, 378
BstFNI CGCG 1 cut(s) 464
BstKTI GATC 1 cut(s) 442
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 1 cut(s) 439
BstNI CCWGG 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 68
BstSFI CTRYAG 1 cut(s) 130
BstUI CGCG 1 cut(s) 464
BsuI GTATCC 1 cut(s) 396
BsuRI GGCC 2 cut(s) 73, 107
BtsCI GGATG 2 cut(s) 277, 378
BtsIMutI CAGTG 3 cut(s) 234, 339, 389
BveI ACCTGC 1 cut(s) 137
Cfr13I GGNCC 1 cut(s) 61
Csp6I GTAC 2 cut(s) 148, 298
CviJI RGCY 9 cut(s) 47, 73, 85, 107, 169, 280, 309, 322, 432
CviKI_1 RGCY 9 cut(s) 47, 73, 85, 107, 169, 280, 309, 322, 432
CviQI GTAC 2 cut(s) 148, 298
DpnI GATC 1 cut(s) 441
DpnII GATC 1 cut(s) 439
EaeI YGGCCR 2 cut(s) 71, 105
Eco24I GRGCYC 1 cut(s) 311
Eco47I GGWCC 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 68
EcoT38I GRGCYC 1 cut(s) 311
FaiI YATR 4 cut(s) 11, 209, 291, 491
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FbaI TGATCA 1 cut(s) 439
FblI GTMKAC 1 cut(s) 114
FokI GGATG 2 cut(s) 284, 385
FriOI GRGCYC 1 cut(s) 311
HaeIII GGCC 2 cut(s) 73, 107
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HindIII AAGCTT 1 cut(s) 430
HinfI GANTC 4 cut(s) 136, 158, 358, 466
HphI GGTGA 2 cut(s) 53, 118
Hpy166II GTNNAC 1 cut(s) 115
Hpy188III TCNNGA 2 cut(s) 418, 449
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 2 cut(s) 229, 302
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 3 cut(s) 132, 183, 376
HpySE526I ACGT 1 cut(s) 117
Ksp22I TGATCA 1 cut(s) 439
Kzo9I GATC 1 cut(s) 439
LpnPI CCDG 5 cut(s) 55, 82, 118, 142, 459
LweI GCATC 1 cut(s) 363
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 223
MalI GATC 1 cut(s) 441
MboI GATC 1 cut(s) 439
MfeI CAATTG 1 cut(s) 163
MhlI GDGCHC 2 cut(s) 24, 311
MlsI TGGCCA 2 cut(s) 73, 107
MluCI AATT 6 cut(s) 152, 163, 190, 380, 452, 502
MluNI TGGCCA 2 cut(s) 73, 107
MlyI GAGTC 2 cut(s) 130, 152
MnlI CCTC 8 cut(s) 27, 37, 75, 262, 270, 394, 397, 504
Mox20I TGGCCA 2 cut(s) 73, 107
MscI TGGCCA 2 cut(s) 73, 107
MseI TTAA 2 cut(s) 459, 505
Msp20I TGGCCA 2 cut(s) 73, 107
MspR9I CCNGG 1 cut(s) 70
MunI CAATTG 1 cut(s) 163
MvaI CCWGG 1 cut(s) 70
MvnI CGCG 1 cut(s) 464
NdeII GATC 1 cut(s) 439
NlaIV GGNNCC 1 cut(s) 63
NmuCI GTSAC 1 cut(s) 223
PaeR7I CTCGAG 1 cut(s) 170
PaqCI CACCTGC 1 cut(s) 137
PfeI GAWTC 2 cut(s) 358, 466
PflMI CCANNNNNTGG 1 cut(s) 395
PleI GAGTC 2 cut(s) 130, 152
PpsI GAGTC 2 cut(s) 130, 152
Psp6I CCWGG 1 cut(s) 68
PspGI CCWGG 1 cut(s) 68
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 1 cut(s) 61
PspXI VCTCGAGB 1 cut(s) 170
PstI CTGCAG 1 cut(s) 134
RsaI GTAC 2 cut(s) 149, 299
RsaNI GTAC 2 cut(s) 148, 298
SalI GTCGAC 1 cut(s) 113
SaqAI TTAA 2 cut(s) 459, 505
Sau3AI GATC 1 cut(s) 439
Sau96I GGNCC 1 cut(s) 61
SbfI CCTGCAGG 1 cut(s) 134
SchI GAGTC 2 cut(s) 130, 152
ScrFI CCNGG 1 cut(s) 70
SdaI CCTGCAGG 1 cut(s) 134
SduI GDGCHC 2 cut(s) 24, 311
SetI ASST 9 cut(s) 38, 120, 131, 214, 331, 352, 408, 434, 496
SfaNI GCATC 1 cut(s) 363
SfcI CTRYAG 1 cut(s) 130
Sfr274I CTCGAG 1 cut(s) 170
SinI GGWCC 1 cut(s) 61
SlaI CTCGAG 1 cut(s) 170
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse8387I CCTGCAGG 1 cut(s) 134
Sse9I AATT 6 cut(s) 152, 163, 190, 380, 452, 502
SsiI CCGC 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 68
TaaI ACNGT 2 cut(s) 229, 302
TaiI ACGT 1 cut(s) 120
TaqI TCGA 4 cut(s) 114, 171, 187, 311
TasI AATT 6 cut(s) 152, 163, 190, 380, 452, 502
TatI WGTACW 1 cut(s) 297
TfiI GAWTC 2 cut(s) 358, 466
Tru1I TTAA 2 cut(s) 459, 505
Tru9I TTAA 2 cut(s) 459, 505
TscAI CASTG 3 cut(s) 234, 346, 396
TseFI GTSAC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 223
TspGWI ACGGA 3 cut(s) 7, 82, 129
TspRI CASTG 3 cut(s) 234, 346, 396
Van91I CCANNNNNTGG 1 cut(s) 395
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 2 cut(s) 190, 452
XhoI CTCGAG 1 cut(s) 170
XmiI GTMKAC 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.