Rroxscaffold_2G00149140

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001616
Physical Location & Seq
Forward (+)
86729877 .. 86730798
922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149140.1

Sequence Viewer

Length: 663 bp
ATGAAGACGGGGCGGCCGATGAATACAATGACATTTCCGGCGAGATGTTCGGCGCTCCGCAAGATGTTAGACGGGCTCGAACAAAGCGACGATGAAATTGAAATGGAGTCGTCTGTGGCTGATGCGAGTCCGCGATTCGGGATGAGGAATTCGGGTGGCGAGAGTGGAGAAGCTCGCGAAGATGTGAAGCGGTTCGGCGAGGAAATGATTGACGATGGCTCTCTCGCCTCTATCTACTATTTCCGAATCGATAAATTTCGAGGTGCGGTGAGGCTTTTCTGCCTCGGACCCTTCCTAGCTAAAACAGAGGCTATTCTCCGTCATTGCTTTGGCCAATGTCGACGTAATAATCACTGCAGGACTCCGTTTGGGTACAAATCGGACTCCAATTGGCTCGAGCAGAGTTGCAATCGAAATTCTCTGTGGTTTTCCATAGGTGGAGAGTTTGTGACGGTGAGTGATGGAGAAGAGTGTAGGCGTGACGGTGAGAAAGAGGATGAGAGGCGTTCGTTATGTAATGTACGGTTCGGGCTCGACGGGGAAGACGAAAGGTGTTTGTGGCAACGACCAAGGTCTTTGGAATCGGTTTCGTGGATGCAAGAATTGTATCCACTGAATGGAAAGGAGGTTTTTGTTGTTCAAGACAGCAATAAGCTTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

220

Amino Acids

25.25

Weight (kDa)

5.32

Isoelectric Point (pI)

62.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 617
AccI GTMKAC 1 cut(s) 340
AccII CGCG 2 cut(s) 133, 177
AciI CCGC 5 cut(s) 13, 58, 131, 190, 266
AcoI YGGCCR 2 cut(s) 14, 331
AcsI RAATTY 3 cut(s) 148, 254, 415
AfaI GTAC 2 cut(s) 374, 522
AfiI CCNNNNNNNGG 2 cut(s) 137, 617
AgsI TTSAA 2 cut(s) 101, 641
AluBI AGCT 3 cut(s) 173, 299, 655
AluI AGCT 3 cut(s) 173, 299, 655
Ama87I CYCGRG 1 cut(s) 395
AoxI GGCC 2 cut(s) 14, 331
ApoI RAATTY 3 cut(s) 148, 254, 415
Asp700I GAANNNNTTC 1 cut(s) 191
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 1 cut(s) 287
AsuHPI GGTGA 3 cut(s) 280, 466, 497
AvaI CYCGRG 1 cut(s) 395
AvaII GGWCC 1 cut(s) 287
BalI TGGCCA 1 cut(s) 333
BanII GRGCYC 2 cut(s) 78, 534
BbsI GAAGAC 2 cut(s) 11, 549
BccI CCATC 2 cut(s) 209, 455
BciVI GTATCC 1 cut(s) 618
BfaI CTAG 1 cut(s) 296
BfmI CTRYAG 1 cut(s) 355
BfoI RGCGCY 1 cut(s) 56
BfuI GTATCC 1 cut(s) 618
BisI GCNGC 1 cut(s) 14
BlsI GCNGC 1 cut(s) 15
Bme18I GGWCC 1 cut(s) 287
BmeT110I CYCGRG 1 cut(s) 395
BmgT120I GGNCC 1 cut(s) 287
BmiI GGNNCC 1 cut(s) 289
BmsI GCATC 2 cut(s) 112, 585
BoxI GACNNNNGTC 1 cut(s) 571
BpiI GAAGAC 2 cut(s) 11, 549
Bsa29I ATCGAT 1 cut(s) 249
BsaJI CCNNGG 2 cut(s) 283, 569
BsaXI ACNNNNNCTCC 2 cut(s) 432, 462
Bsc4I CCNNNNNNNGG 2 cut(s) 137, 617
Bse3DI GCAATG 1 cut(s) 322
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 2 cut(s) 283, 569
BseGI GGATG 3 cut(s) 147, 502, 600
BseLI CCNNNNNNNGG 2 cut(s) 137, 617
BseMI GCAATG 1 cut(s) 322
BseX3I CGGCCG 1 cut(s) 14
Bsh1236I CGCG 2 cut(s) 133, 177
Bsh1285I CGRYCG 1 cut(s) 17
BshFI GGCC 2 cut(s) 16, 333
BshVI ATCGAT 1 cut(s) 249
BsiEI CGRYCG 1 cut(s) 17
BsiHKCI CYCGRG 1 cut(s) 395
BsiSI CCGG 1 cut(s) 38
BslI CCNNNNNNNGG 2 cut(s) 137, 617
BsnI GGCC 2 cut(s) 16, 333
BsoBI CYCGRG 1 cut(s) 395
Bsp1286I GDGCHC 2 cut(s) 78, 534
Bsp68I TCGCGA 1 cut(s) 177
BspACI CCGC 5 cut(s) 13, 58, 131, 190, 266
BspANI GGCC 2 cut(s) 16, 333
BspDI ATCGAT 1 cut(s) 249
BspFNI CGCG 2 cut(s) 133, 177
BspLI GGNNCC 1 cut(s) 289
BspMAI CTGCAG 1 cut(s) 359
BsrDI GCAATG 1 cut(s) 322
BssECI CCNNGG 2 cut(s) 283, 569
BssT1I CCWWGG 1 cut(s) 569
Bst4CI ACNGT 3 cut(s) 454, 485, 525
Bst6I CTCTTC 1 cut(s) 462
BstC8I GCNNGC 1 cut(s) 175
BstF5I GGATG 3 cut(s) 147, 502, 600
BstFNI CGCG 2 cut(s) 133, 177
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 1 cut(s) 55
BstMCI CGRYCG 1 cut(s) 17
BstPAI GACNNNNGTC 1 cut(s) 571
BstSFI CTRYAG 1 cut(s) 355
BstUI CGCG 2 cut(s) 133, 177
BstV2I GAAGAC 2 cut(s) 11, 549
BstZI CGGCCG 1 cut(s) 14
Bsu15I ATCGAT 1 cut(s) 249
BsuI GTATCC 1 cut(s) 618
BsuRI GGCC 2 cut(s) 16, 333
BsuTUI ATCGAT 1 cut(s) 249
BtsCI GGATG 3 cut(s) 147, 502, 600
BtsI GCAGTG 1 cut(s) 352
BtsIMutI CAGTG 2 cut(s) 352, 611
BtuMI TCGCGA 1 cut(s) 177
Cac8I GCNNGC 1 cut(s) 175
CfoI GCGC 1 cut(s) 55
Cfr13I GGNCC 1 cut(s) 287
ClaI ATCGAT 1 cut(s) 249
Csp6I GTAC 2 cut(s) 373, 521
CviQI GTAC 2 cut(s) 373, 521
EaeI YGGCCR 2 cut(s) 14, 331
EagI CGGCCG 1 cut(s) 14
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
EclXI CGGCCG 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 569
Eco24I GRGCYC 2 cut(s) 78, 534
Eco47I GGWCC 1 cut(s) 287
Eco52I CGGCCG 1 cut(s) 14
Eco88I CYCGRG 1 cut(s) 395
EcoRI GAATTC 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 569
EcoT38I GRGCYC 2 cut(s) 78, 534
ErhI CCWWGG 1 cut(s) 569
FaiI YATR 2 cut(s) 434, 514
FblI GTMKAC 1 cut(s) 340
Fnu4HI GCNGC 1 cut(s) 14
FokI GGATG 3 cut(s) 154, 509, 607
FriOI GRGCYC 2 cut(s) 78, 534
Fsp4HI GCNGC 1 cut(s) 14
FspBI CTAG 1 cut(s) 296
GlaI GCGC 1 cut(s) 54
GluI GCNGC 1 cut(s) 14
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 2 cut(s) 16, 333
HapII CCGG 1 cut(s) 38
HhaI GCGC 1 cut(s) 55
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HincII GTYRAC 1 cut(s) 341
HindII GTYRAC 1 cut(s) 341
HindIII AAGCTT 1 cut(s) 653
HinfI GANTC 7 cut(s) 107, 127, 135, 246, 361, 383, 581
HpaII CCGG 1 cut(s) 38
HphI GGTGA 3 cut(s) 280, 466, 497
Hpy166II GTNNAC 1 cut(s) 341
Hpy188I TCNGA 3 cut(s) 245, 287, 382
Hpy188III TCNNGA 3 cut(s) 139, 176, 641
Hpy8I GTNNAC 1 cut(s) 341
Hpy99I CGWCG 3 cut(s) 92, 345, 539
HpyAV CCTTC 1 cut(s) 301
HpyCH4III ACNGT 3 cut(s) 454, 485, 525
HpyCH4IV ACGT 1 cut(s) 343
HpyCH4V TGCA 3 cut(s) 357, 408, 598
HpySE526I ACGT 1 cut(s) 343
HspAI GCGC 1 cut(s) 53
LmnI GCTCC 1 cut(s) 60
LpnPI CCDG 2 cut(s) 51, 343
LweI GCATC 2 cut(s) 112, 585
MaeI CTAG 1 cut(s) 296
MaeII ACGT 1 cut(s) 343
MaeIII GTNAC 2 cut(s) 448, 479
MboII GAAGA 4 cut(s) 16, 191, 479, 554
MfeI CAATTG 1 cut(s) 388
MhlI GDGCHC 2 cut(s) 78, 534
MlsI TGGCCA 1 cut(s) 333
MluCI AATT 6 cut(s) 96, 148, 254, 388, 415, 602
MluNI TGGCCA 1 cut(s) 333
MlyI GAGTC 4 cut(s) 116, 136, 355, 377
Mox20I TGGCCA 1 cut(s) 333
MroXI GAANNNNTTC 1 cut(s) 191
MscI TGGCCA 1 cut(s) 333
Msp20I TGGCCA 1 cut(s) 333
MspI CCGG 1 cut(s) 38
MunI CAATTG 1 cut(s) 388
MvnI CGCG 2 cut(s) 133, 177
NlaIV GGNNCC 1 cut(s) 289
NmuCI GTSAC 2 cut(s) 448, 479
NruI TCGCGA 1 cut(s) 177
PaeR7I CTCGAG 1 cut(s) 395
PcsI WCGNNNNNNNCGW 3 cut(s) 14, 84, 543
PdmI GAANNNNTTC 1 cut(s) 191
PfeI GAWTC 3 cut(s) 135, 246, 581
PflMI CCANNNNNTGG 1 cut(s) 617
PkrI GCNGC 1 cut(s) 15
PleI GAGTC 4 cut(s) 115, 135, 355, 377
PpsI GAGTC 4 cut(s) 115, 135, 355, 377
PshAI GACNNNNGTC 1 cut(s) 571
PspN4I GGNNCC 1 cut(s) 289
PspPI GGNCC 1 cut(s) 287
PspXI VCTCGAGB 1 cut(s) 395
PstI CTGCAG 1 cut(s) 359
RruI TCGCGA 1 cut(s) 177
RsaI GTAC 2 cut(s) 374, 522
RsaNI GTAC 2 cut(s) 373, 521
SalI GTCGAC 1 cut(s) 339
SatI GCNGC 1 cut(s) 14
Sau96I GGNCC 1 cut(s) 287
SchI GAGTC 4 cut(s) 116, 136, 355, 377
SduI GDGCHC 2 cut(s) 78, 534
SetI ASST 9 cut(s) 175, 265, 301, 346, 439, 554, 575, 630, 657
SfaNI GCATC 2 cut(s) 112, 585
SfcI CTRYAG 1 cut(s) 355
Sfr274I CTCGAG 1 cut(s) 395
SinI GGWCC 1 cut(s) 287
SlaI CTCGAG 1 cut(s) 395
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 6 cut(s) 96, 148, 254, 388, 415, 602
SsiI CCGC 5 cut(s) 13, 58, 131, 190, 266
SspMI CTAG 1 cut(s) 296
StyI CCWWGG 1 cut(s) 569
TaaI ACNGT 3 cut(s) 454, 485, 525
TaiI ACGT 1 cut(s) 346
TaqI TCGA 7 cut(s) 78, 249, 259, 340, 396, 412, 534
TasI AATT 6 cut(s) 96, 148, 254, 388, 415, 602
TauI GCSGC 1 cut(s) 16
TfiI GAWTC 3 cut(s) 135, 246, 581
TscAI CASTG 2 cut(s) 359, 618
TseFI GTSAC 2 cut(s) 448, 479
Tsp45I GTSAC 2 cut(s) 448, 479
TspDTI ATGAA 3 cut(s) 17, 35, 108
TspGWI ACGGA 2 cut(s) 308, 354
TspRI CASTG 2 cut(s) 359, 618
Van91I CCANNNNNTGG 1 cut(s) 617
VpaK11BI GGWCC 1 cut(s) 287
XapI RAATTY 3 cut(s) 148, 254, 415
XhoI CTCGAG 1 cut(s) 395
XmiI GTMKAC 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 191
XspI CTAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.