Rmu_co8212272.1_g000001

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_co8212272.1
OG0001616
Physical Location & Seq
Reverse (-)
2 .. 659
658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_co8212272.1_g000001.1.cds

Sequence Viewer

Length: 658 bp
atgagaagccatgaaggttctatactccaccacttctccaaagcagcgtcccaaaacctcaacaaaatcgcagtcatacacgcctccggtgatcgccgtcgtacccgcacctccgtcaccgtccaacctctctacgacggcgaccgcttcttcacgtacgcagagttaatctccgccatcgattccctcgcttcgcagctccgtcgcaataaccttaattcagcttcatcagagctcagaccgagaattctcggcatatatatgccgccgtcggtggaatacatagtctccgtgctctcggttttgaggtgcggagaggcgtttctgcctctggacccgtcctggccaaaacagaggctgctctccgtcgttgcttcagccaatgtcgacctaattatcacctgcacgacgctgttcggttacgaattggactccaattggctggaaaagaattccaatcgtagctttctgtggttttccatgggtggtggagagcctgtgacagtgagtgatggagagcagcgtaagagtgagaaagagaggctcttctgttatgtaatgtacacttcgggctcgacggggaagccgaaaggtgtctgtggaactgaacaaggtctttggaatcggtttcggtggatgcaagaattgtatccattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

219

Amino Acids

24.73

Weight (kDa)

8.91

Isoelectric Point (pI)

54.29

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 410
Acc36I ACCTGC 1 cut(s) 410
AccI GTMKAC 1 cut(s) 387
AciI CCGC 5 cut(s) 106, 145, 174, 266, 312
AcoI YGGCCR 1 cut(s) 344
AcsI RAATTY 2 cut(s) 246, 451
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 3 cut(s) 103, 158, 563
AjnI CCWGG 1 cut(s) 341
AluBI AGCT 4 cut(s) 199, 224, 235, 465
AluI AGCT 4 cut(s) 199, 224, 235, 465
Alw21I GWGCWC 2 cut(s) 237, 297
Alw26I GTCTC 1 cut(s) 292
AlwNI CAGNNNCTG 1 cut(s) 358
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 4 cut(s) 44, 196, 358, 520
ApoI RAATTY 2 cut(s) 246, 451
ArsI GACNNNNNNTTYG 2 cut(s) 57, 89
AspS9I GGNCC 1 cut(s) 334
AsuHPI GGTGA 3 cut(s) 101, 109, 391
AvaII GGWCC 1 cut(s) 334
BalI TGGCCA 1 cut(s) 346
BanII GRGCYC 2 cut(s) 237, 575
BarI GAAGNNNNNNTAC 1 cut(s) 38
Bbv12I GWGCWC 2 cut(s) 237, 297
BbvI GCAGC 4 cut(s) 56, 208, 345, 532
BccI CCATC 2 cut(s) 185, 506
BceAI ACGGC 3 cut(s) 81, 154, 253
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 1 cut(s) 343
BcoDI GTCTC 1 cut(s) 292
BfuAI ACCTGC 1 cut(s) 410
BisI GCNGC 5 cut(s) 45, 197, 266, 359, 521
BlsI GCNGC 5 cut(s) 46, 198, 267, 360, 522
Bme1390I CCNGG 1 cut(s) 343
Bme18I GGWCC 1 cut(s) 334
BmgT120I GGNCC 1 cut(s) 334
BmiI GGNNCC 1 cut(s) 336
BmrFI CCNGG 1 cut(s) 343
BmsI GCATC 1 cut(s) 627
BplI GAGNNNNNCTC 2 cut(s) 155, 187
Bsa29I ATCGAT 1 cut(s) 180
BsaAI YACGTR 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 480
BsaWI WCCGGW 1 cut(s) 86
BsaXI ACNNNNNCTCC 6 cut(s) 20, 50, 272, 302, 483, 513
BseBI CCWGG 1 cut(s) 343
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 480
BseGI GGATG 1 cut(s) 642
BseMII CTCAG 1 cut(s) 250
BseXI GCAGC 4 cut(s) 56, 208, 345, 532
BsgI GTGCAG 1 cut(s) 388
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 1 cut(s) 346
BshVI ATCGAT 1 cut(s) 180
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 2 cut(s) 237, 297
BsiSI CCGG 1 cut(s) 87
BsiWI CGTACG 1 cut(s) 156
BslFI GGGAC 1 cut(s) 34
BsmAI GTCTC 1 cut(s) 292
BsmFI GGGAC 1 cut(s) 34
BsnI GGCC 1 cut(s) 346
Bsp1286I GDGCHC 3 cut(s) 237, 297, 575
Bsp1407I TGTACA 1 cut(s) 561
Bsp143I GATC 1 cut(s) 91
Bsp19I CCATGG 1 cut(s) 480
BspACI CCGC 5 cut(s) 106, 145, 174, 266, 312
BspANI GGCC 1 cut(s) 346
BspCNI CTCAG 1 cut(s) 249
BspDI ATCGAT 1 cut(s) 180
BspLI GGNNCC 1 cut(s) 336
BspMI ACCTGC 1 cut(s) 410
BspQI GCTCTTC 1 cut(s) 551
BsrGI TGTACA 1 cut(s) 561
BssECI CCNNGG 1 cut(s) 480
BssMI GATC 1 cut(s) 91
BssT1I CCWWGG 1 cut(s) 480
Bst2UI CCWGG 1 cut(s) 343
Bst4CI ACNGT 2 cut(s) 121, 505
Bst6I CTCTTC 1 cut(s) 551
BstAUI TGTACA 1 cut(s) 561
BstBAI YACGTR 1 cut(s) 156
BstDEI CTNAG 1 cut(s) 236
BstDSI CCRYGG 1 cut(s) 480
BstF5I GGATG 1 cut(s) 642
BstKTI GATC 1 cut(s) 94
BstMAI GTCTC 1 cut(s) 292
BstMBI GATC 1 cut(s) 91
BstMCI CGRYCG 1 cut(s) 145
BstNI CCWGG 1 cut(s) 343
BstSCI CCNGG 1 cut(s) 341
BstV1I GCAGC 4 cut(s) 56, 208, 345, 532
BstXI CCANNNNNNTGG 1 cut(s) 442
Bsu15I ATCGAT 1 cut(s) 180
BsuRI GGCC 1 cut(s) 346
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 480
BtsCI GGATG 1 cut(s) 642
BtsIMutI CAGTG 1 cut(s) 510
BveI ACCTGC 1 cut(s) 410
CaiI CAGNNNCTG 1 cut(s) 358
Cfr13I GGNCC 1 cut(s) 334
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 2 cut(s) 36, 418
Csp6I GTAC 3 cut(s) 102, 157, 562
CviAII CATG 2 cut(s) 11, 481
CviQI GTAC 3 cut(s) 102, 157, 562
DdeI CTNAG 1 cut(s) 236
DpnI GATC 1 cut(s) 93
DpnII GATC 1 cut(s) 91
EaeI YGGCCR 1 cut(s) 344
Eam1104I CTCTTC 1 cut(s) 551
EarI CTCTTC 1 cut(s) 551
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 480
Eco24I GRGCYC 2 cut(s) 237, 575
Eco47I GGWCC 1 cut(s) 334
Eco53kI GAGCTC 1 cut(s) 235
Eco57I CTGAAG 1 cut(s) 360
EcoICRI GAGCTC 1 cut(s) 235
EcoRI GAATTC 2 cut(s) 246, 451
EcoRII CCWGG 1 cut(s) 341
EcoT14I CCWWGG 1 cut(s) 480
EcoT38I GRGCYC 2 cut(s) 237, 575
ErhI CCWWGG 1 cut(s) 480
FaeI CATG 2 cut(s) 14, 484
FaqI GGGAC 1 cut(s) 34
FatI CATG 2 cut(s) 10, 480
FauI CCCGC 1 cut(s) 113
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 5 cut(s) 45, 197, 266, 359, 521
FokI GGATG 1 cut(s) 649
FriOI GRGCYC 2 cut(s) 237, 575
Fsp4HI GCNGC 5 cut(s) 45, 197, 266, 359, 521
GluI GCNGC 5 cut(s) 45, 197, 266, 359, 521
HaeIII GGCC 1 cut(s) 346
HapII CCGG 1 cut(s) 87
HgaI GACGC 2 cut(s) 36, 418
Hin1II CATG 2 cut(s) 14, 484
HincII GTYRAC 1 cut(s) 388
HindII GTYRAC 1 cut(s) 388
HinfI GANTC 3 cut(s) 182, 431, 622
HpaII CCGG 1 cut(s) 87
HphI GGTGA 3 cut(s) 101, 109, 391
Hpy166II GTNNAC 2 cut(s) 388, 564
Hpy188I TCNGA 2 cut(s) 232, 239
Hpy188III TCNNGA 1 cut(s) 332
Hpy8I GTNNAC 2 cut(s) 388, 564
Hpy99I CGWCG 7 cut(s) 102, 140, 207, 274, 371, 412, 580
HpyAV CCTTC 1 cut(s) 8
HpyCH4III ACNGT 2 cut(s) 121, 505
HpyCH4IV ACGT 1 cut(s) 155
HpyCH4V TGCA 2 cut(s) 405, 640
HpyF3I CTNAG 1 cut(s) 236
HpySE526I ACGT 1 cut(s) 155
Hsp92II CATG 2 cut(s) 14, 484
Kzo9I GATC 1 cut(s) 91
LguI GCTCTTC 1 cut(s) 551
LmnI GCTCC 1 cut(s) 204
LpnPI CCDG 7 cut(s) 100, 317, 328, 355, 415, 428, 510
Lsp1109I GCAGC 4 cut(s) 56, 208, 345, 532
LweI GCATC 1 cut(s) 627
MaeII ACGT 1 cut(s) 155
MaeIII GTNAC 3 cut(s) 115, 419, 499
MalI GATC 1 cut(s) 93
MboI GATC 1 cut(s) 91
MboII GAAGA 2 cut(s) 142, 538
MfeI CAATTG 1 cut(s) 436
MhlI GDGCHC 3 cut(s) 237, 297, 575
MlsI TGGCCA 1 cut(s) 346
MluCI AATT 7 cut(s) 217, 246, 393, 425, 436, 451, 644
MluNI TGGCCA 1 cut(s) 346
MlyI GAGTC 1 cut(s) 425
MmeI TCCRAC 1 cut(s) 148
Mox20I TGGCCA 1 cut(s) 346
MscI TGGCCA 1 cut(s) 346
MseI TTAA 2 cut(s) 167, 216
MslI CAYNNNNRTG 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 346
MspI CCGG 1 cut(s) 87
MspR9I CCNGG 1 cut(s) 343
MunI CAATTG 1 cut(s) 436
MvaI CCWGG 1 cut(s) 343
NcoI CCATGG 1 cut(s) 480
NdeII GATC 1 cut(s) 91
NlaIII CATG 2 cut(s) 14, 484
NlaIV GGNNCC 1 cut(s) 336
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 2 cut(s) 115, 499
PaqCI CACCTGC 1 cut(s) 410
PciSI GCTCTTC 1 cut(s) 551
PcsI WCGNNNNNNNCGW 1 cut(s) 584
PfeI GAWTC 2 cut(s) 182, 622
Pfl23II CGTACG 1 cut(s) 156
PkrI GCNGC 5 cut(s) 46, 198, 267, 360, 522
PleI GAGTC 1 cut(s) 425
PpsI GAGTC 1 cut(s) 425
Ppu21I YACGTR 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 341
PspGI CCWGG 1 cut(s) 341
PspLI CGTACG 1 cut(s) 156
PspN4I GGNNCC 1 cut(s) 336
PspPI GGNCC 1 cut(s) 334
PstNI CAGNNNCTG 1 cut(s) 358
RsaI GTAC 3 cut(s) 103, 158, 563
RsaNI GTAC 3 cut(s) 102, 157, 562
RseI CAYNNNNRTG 1 cut(s) 260
SacI GAGCTC 1 cut(s) 237
SalI GTCGAC 1 cut(s) 386
SapI GCTCTTC 1 cut(s) 551
SaqAI TTAA 2 cut(s) 167, 216
SatI GCNGC 5 cut(s) 45, 197, 266, 359, 521
Sau3AI GATC 1 cut(s) 91
Sau96I GGNCC 1 cut(s) 334
SchI GAGTC 1 cut(s) 425
ScrFI CCNGG 1 cut(s) 343
SduI GDGCHC 3 cut(s) 237, 297, 575
SfaNI GCATC 1 cut(s) 627
SinI GGWCC 1 cut(s) 334
SmiMI CAYNNNNRTG 1 cut(s) 260
Sse9I AATT 7 cut(s) 217, 246, 393, 425, 436, 451, 644
SsiI CCGC 5 cut(s) 106, 145, 174, 266, 312
SstI GAGCTC 1 cut(s) 237
StyD4I CCNGG 1 cut(s) 341
StyI CCWWGG 1 cut(s) 480
TaaI ACNGT 2 cut(s) 121, 505
TaiI ACGT 1 cut(s) 158
TaqI TCGA 3 cut(s) 180, 387, 575
TaqII GACCGA 1 cut(s) 256
TasI AATT 7 cut(s) 217, 246, 393, 425, 436, 451, 644
TatI WGTACW 1 cut(s) 561
TauI GCSGC 1 cut(s) 268
TfiI GAWTC 2 cut(s) 182, 622
Tru1I TTAA 2 cut(s) 167, 216
Tru9I TTAA 2 cut(s) 167, 216
TscAI CASTG 1 cut(s) 510
TseFI GTSAC 2 cut(s) 115, 499
TseI GCWGC 4 cut(s) 44, 196, 358, 520
Tsp45I GTSAC 2 cut(s) 115, 499
TspDTI ATGAA 2 cut(s) 27, 216
TspGWI ACGGA 4 cut(s) 103, 191, 280, 355
TspRI CASTG 1 cut(s) 510
VpaK11BI GGWCC 1 cut(s) 334
XapI RAATTY 2 cut(s) 246, 451
XmiI GTMKAC 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.