Rmu_sc0002160.1_g000017

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002160.1
OG0001616
Physical Location & Seq
Forward (+)
82906 .. 83358
453 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002160.1_g000017.1.cds

Sequence Viewer

Length: 453 bp
atgccgcctttggtggaatacatagtctccgtgctctcggttttgaggtgcggcgaggcgtttctgcatctggacccgtcctggcaaaaacagaggctgctctccgtcgttgcttcggccaatgtcgacctaattatcacctgcacgacgccgtttggttacgaattggactccaattggctggaaaagaatatcaatcgtagctttctgtggttttccatgggtggtggggagtctgtgacaaagagtgatggagagcagcgtaagagtgagaacgagaggctgttctgttatgtaatgtacacttcgggctcgatggggaagccgaaaggtgtctgtggaactgaacaaggtctttggaatcggtttcggtggatgcaagaattgtatccattgaatggagaggaggttttgttgttcaagacagcaataagctttgctgatcacttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

150

Amino Acids

17.08

Weight (kDa)

5.4

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 149
Acc36I ACCTGC 1 cut(s) 149
AccB7I CCANNNNNTGG 1 cut(s) 398
AccI GTMKAC 1 cut(s) 126
AciI CCGC 2 cut(s) 5, 51
AcoI YGGCCR 1 cut(s) 117
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 1 cut(s) 302
AfiI CCNNNNNNNGG 1 cut(s) 398
AgsI TTSAA 2 cut(s) 397, 421
AjnI CCWGG 1 cut(s) 80
AluBI AGCT 2 cut(s) 204, 435
AluI AGCT 2 cut(s) 204, 435
Alw21I GWGCWC 1 cut(s) 36
Alw26I GTCTC 1 cut(s) 31
AlwNI CAGNNNCTG 1 cut(s) 97
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 2 cut(s) 97, 259
AspS9I GGNCC 1 cut(s) 73
AsuHPI GGTGA 1 cut(s) 130
AvaII GGWCC 1 cut(s) 73
BanII GRGCYC 1 cut(s) 314
Bbv12I GWGCWC 1 cut(s) 36
BbvI GCAGC 2 cut(s) 84, 271
BccI CCATC 2 cut(s) 245, 310
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 1 cut(s) 82
BciVI GTATCC 1 cut(s) 399
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 1 cut(s) 31
BfuAI ACCTGC 1 cut(s) 149
BfuI GTATCC 1 cut(s) 399
BisI GCNGC 4 cut(s) 5, 52, 98, 260
BlsI GCNGC 4 cut(s) 6, 53, 99, 261
Bme1390I CCNGG 1 cut(s) 82
Bme18I GGWCC 1 cut(s) 73
BmgT120I GGNCC 1 cut(s) 73
BmiI GGNNCC 1 cut(s) 75
BmrFI CCNGG 1 cut(s) 82
BmsI GCATC 2 cut(s) 76, 366
BsaHI GRCGYC 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 219
BsaXI ACNNNNNCTCC 6 cut(s) 11, 41, 393, 398, 423, 428
Bsc4I CCNNNNNNNGG 1 cut(s) 398
BseBI CCWGG 1 cut(s) 82
BseDI CCNNGG 1 cut(s) 219
BseGI GGATG 1 cut(s) 381
BseLI CCNNNNNNNGG 1 cut(s) 398
BseRI GAGGAG 1 cut(s) 419
BseXI GCAGC 2 cut(s) 84, 271
BsgI GTGCAG 1 cut(s) 127
BshFI GGCC 1 cut(s) 119
BsiHKAI GWGCWC 1 cut(s) 36
BslI CCNNNNNNNGG 1 cut(s) 398
BsmAI GTCTC 1 cut(s) 31
BsnI GGCC 1 cut(s) 119
Bsp1286I GDGCHC 2 cut(s) 36, 314
Bsp1407I TGTACA 1 cut(s) 300
Bsp143I GATC 1 cut(s) 442
Bsp19I CCATGG 1 cut(s) 219
BspACI CCGC 2 cut(s) 5, 51
BspANI GGCC 1 cut(s) 119
BspLI GGNNCC 1 cut(s) 75
BspMI ACCTGC 1 cut(s) 149
BsrGI TGTACA 1 cut(s) 300
BssECI CCNNGG 1 cut(s) 219
BssMI GATC 1 cut(s) 442
BssNI GRCGYC 1 cut(s) 149
BssT1I CCWWGG 1 cut(s) 219
Bst2UI CCWGG 1 cut(s) 82
BstACI GRCGYC 1 cut(s) 149
BstAUI TGTACA 1 cut(s) 300
BstDSI CCRYGG 1 cut(s) 219
BstF5I GGATG 1 cut(s) 381
BstKTI GATC 1 cut(s) 445
BstMAI GTCTC 1 cut(s) 31
BstMBI GATC 1 cut(s) 442
BstNI CCWGG 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 80
BstV1I GCAGC 2 cut(s) 84, 271
BstXI CCANNNNNNTGG 1 cut(s) 181
BsuI GTATCC 1 cut(s) 399
BsuRI GGCC 1 cut(s) 119
BtgI CCRYGG 1 cut(s) 219
BtsCI GGATG 1 cut(s) 381
BveI ACCTGC 1 cut(s) 149
CaiI CAGNNNCTG 1 cut(s) 97
Cfr13I GGNCC 1 cut(s) 73
CseI GACGC 1 cut(s) 157
Csp6I GTAC 1 cut(s) 301
CviAII CATG 1 cut(s) 220
CviJI RGCY 8 cut(s) 97, 119, 181, 204, 283, 312, 325, 435
CviKI_1 RGCY 8 cut(s) 97, 119, 181, 204, 283, 312, 325, 435
CviQI GTAC 1 cut(s) 301
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
EaeI YGGCCR 1 cut(s) 117
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 80
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 1 cut(s) 314
ErhI CCWWGG 1 cut(s) 219
FaeI CATG 1 cut(s) 223
FaiI YATR 3 cut(s) 23, 221, 294
FatI CATG 1 cut(s) 219
FbaI TGATCA 1 cut(s) 442
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 4 cut(s) 5, 52, 98, 260
FokI GGATG 1 cut(s) 388
FriOI GRGCYC 1 cut(s) 314
Fsp4HI GCNGC 4 cut(s) 5, 52, 98, 260
GluI GCNGC 4 cut(s) 5, 52, 98, 260
HaeIII GGCC 1 cut(s) 119
HgaI GACGC 1 cut(s) 157
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 1 cut(s) 223
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HindIII AAGCTT 1 cut(s) 433
HinfI GANTC 3 cut(s) 170, 233, 361
HphI GGTGA 1 cut(s) 130
Hpy166II GTNNAC 2 cut(s) 127, 303
Hpy188III TCNNGA 2 cut(s) 71, 421
Hpy8I GTNNAC 2 cut(s) 127, 303
Hpy99I CGWCG 2 cut(s) 110, 151
HpyCH4V TGCA 3 cut(s) 67, 144, 379
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 1 cut(s) 223
Ksp22I TGATCA 1 cut(s) 442
Kzo9I GATC 1 cut(s) 442
LpnPI CCDG 5 cut(s) 56, 67, 94, 154, 167
Lsp1109I GCAGC 2 cut(s) 84, 271
LweI GCATC 2 cut(s) 76, 366
MaeIII GTNAC 2 cut(s) 158, 238
MalI GATC 1 cut(s) 444
MboI GATC 1 cut(s) 442
MfeI CAATTG 1 cut(s) 175
MhlI GDGCHC 2 cut(s) 36, 314
MluCI AATT 4 cut(s) 132, 164, 175, 383
MlyI GAGTC 2 cut(s) 164, 242
MnlI CCTC 6 cut(s) 39, 49, 87, 273, 397, 400
MspR9I CCNGG 1 cut(s) 82
MunI CAATTG 1 cut(s) 175
MvaI CCWGG 1 cut(s) 82
NcoI CCATGG 1 cut(s) 219
NdeII GATC 1 cut(s) 442
NlaIII CATG 1 cut(s) 223
NlaIV GGNNCC 1 cut(s) 75
NmuCI GTSAC 1 cut(s) 238
PaqCI CACCTGC 1 cut(s) 149
PfeI GAWTC 1 cut(s) 361
PflMI CCANNNNNTGG 1 cut(s) 398
PkrI GCNGC 4 cut(s) 6, 53, 99, 261
PleI GAGTC 2 cut(s) 164, 241
PpsI GAGTC 2 cut(s) 164, 241
Psp6I CCWGG 1 cut(s) 80
PspGI CCWGG 1 cut(s) 80
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 1 cut(s) 73
PstNI CAGNNNCTG 1 cut(s) 97
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
SalI GTCGAC 1 cut(s) 125
SatI GCNGC 4 cut(s) 5, 52, 98, 260
Sau3AI GATC 1 cut(s) 442
Sau96I GGNCC 1 cut(s) 73
SchI GAGTC 2 cut(s) 164, 242
ScrFI CCNGG 1 cut(s) 82
SduI GDGCHC 2 cut(s) 36, 314
SetI ASST 8 cut(s) 50, 132, 143, 206, 334, 355, 411, 437
SfaNI GCATC 2 cut(s) 76, 366
SinI GGWCC 1 cut(s) 73
Sse9I AATT 4 cut(s) 132, 164, 175, 383
SsiI CCGC 2 cut(s) 5, 51
StyD4I CCNGG 1 cut(s) 80
StyI CCWWGG 1 cut(s) 219
TaqI TCGA 2 cut(s) 126, 314
TasI AATT 4 cut(s) 132, 164, 175, 383
TatI WGTACW 1 cut(s) 300
TauI GCSGC 2 cut(s) 7, 54
TfiI GAWTC 1 cut(s) 361
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 2 cut(s) 97, 259
Tsp45I GTSAC 1 cut(s) 238
TspGWI ACGGA 2 cut(s) 19, 94
Van91I CCANNNNNTGG 1 cut(s) 398
VpaK11BI GGWCC 1 cut(s) 73
XmiI GTMKAC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.