Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000002

Orthogroup ID

OG0001616

Physical Location & Seq

Genomic Coordinates Reverse (-)

22243699 .. 22245314

Gene Structure 1616 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug02G0226400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1140 bp

ATGAAGCTACATAGCTTTCTTCTTCGTCTTCTGTGTCTGGTTTTACTGAAGTACACTGACGTCAATGCGCAGCAGTCGCCGGGGCCGATGCAGACCAACGGCTATGCCTACCTCGAGAACTTCAACTCCTCGATGGCCCTGGTCATCGTGTTCCTCGTGTGCGCCTTCTTCATCGTCGGATTCTTCTCCATCTACATTCGCCGTTGCGCCGAGACGCACATGGCCAACGCAGGAGGGTCCCTGGGCTCAGCGGCTGCCATTGGAATAGCCGGACGACGCAGAGGCGTCGACCCGGCTGTGATTGAGAAGTTTCCGATGTTCGTCTACTCCGACGTCAAGGATCTCAAGATTGGCAAAGGGGCTCTGGAGTGCGCGGTGTGCTTGAGCGAGTTTGAGGACTACGAAACGCTGCGATTGCTGCCGAAATGCCACCACGTATTCCATCCCGACTGCATCGACGCGTGGTTGGCCTCGCACATCACGTGTCCGGTTTGCCGCGCAAAGCTGACGCCCGACTCAAATCACCCACTTTCCGAGCCAAATAACAATTCGAATCACCAAGAAAGTACTGGTACAAATGAAATTAGTGATGATCAGCAACAGAACGACGCGGTTGTTGTGAATGTGAGTGAAAACCAGAGCAGCGAAGCGATTGAGATAGAGAACCGTCCGGCGAGATCTGGAATATTCGGGAAGTTTCCAAGGTCACACTCGACGGGTCACTCGGTAGTTCAACCCGGAGAGAACACGGAGCGGTACACGTTGAGACTGCCGGATTCAGTGAGGAGGCAGCTGGTTTCGAGCCGGAACAATAAGCTCAAGCGGTCGAGTAGCTACGACGTCGTTCTACCGCGGCTTGGAAGTTCAAGAAAAGGCTATAGATACGGTGGCGAAGGGAGTAGTAGCAGTAGAGGGAAAATTAGTCTTGATCATCAAAACGGGCGGTTCGACCCGTGGGTGTTGTTGAAAACGCCACCGTTCTTTGGTAGGGGTGGTGGTTCCTTTAAGTCTCCTAAGAATGGTGTTGACGGAGATGGGTTGTTTGTAAAGCCTTTGTTAACGCCGGTGAAAATTTCGTTAGATTGTCAGAATGTCAAAGCCAACAGGTCTCAAGAACCGATGTCTCGGCCTCCGGTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000373 GO:0000375 GO:0000377 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0006810 GO:0007275 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0008380 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009790 GO:0009791 GO:0009793 GO:0009892 GO:0009987 GO:0010154 GO:0010467 GO:0010468 GO:0010494 GO:0010496 GO:0010497 GO:0010501 GO:0010605 GO:0010608 GO:0010629 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019222 GO:0022414 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0034641 GO:0035770 GO:0036464 GO:0040029 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0048316 GO:0048519 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0051179 GO:0051234 GO:0060255 GO:0061458 GO:0065007 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0097159 GO:0140098 GO:1901360 GO:1901363 GO:1990904

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD DSHCT Helicase_C

Protein Analysis

379

Amino Acids

41.77

Weight (kDa)

8.94

Isoelectric Point (pI)

50.98

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	123 - 166	2e-13	Ring finger domain
zf-RING_11	PF17123	123 - 152	5.6e-10	RING-like zinc finger
zf-C3HC4_2	PF13923	123 - 165	1.6e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	124 - 165	4.1e-06	Zinc finger, C3HC4 type (RING finger)
zf-rbx1	PF12678	136 - 166	2.3e-06	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001616)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr2g0102891
rosa_laevigata	RLG00000003325 RLG00000010663 RLG00000014743 RLG00000021608 RLG00000028731
rosa_multiflora	Rmu_co8212272.1_g000001 Rmu_co8257291.1_g000001 Rmu_sc0000375.1_g000004 Rmu_sc0001619.1_g000004 Rmu_sc0002160.1_g000017 Rmu_sc0002960.1_g000003 Rmu_sc0004031.1_g000010 Rmu_sc0011657.1_g000008 Rmu_sc0014046.1_g000004
rosa_roxburghii	Rroxscaffold_1G00024170 Rroxscaffold_2G00149140 Rroxscaffold_5G00382470
rosa_rugosa	Rorug02G0226400 Rorug02G0226500 Rorug04G0046600 Rorug05G0408800 Rorug05G0408900 Rorug06G0089500 Rorug06G0089600 Rorug06G0089700.1 Rorug06G0089800
rosa_samantha	Rh1BG120900 Rh2AG219500 Rh2AG390100 Rh2BG229500 Rh2CG165000 Rh2DG225900 Rh2DG381600 Rh3BG369300 Rh3CG331600 Rh3DG328600 Rh4AG016000 Rh4AG058100 Rh4BG056900 Rh4CG112600 Rh4CG219300 Rh4CG219400 Rh4DG021700 Rh5DG477800 Rh5DG495600 Rh7AG221500 Rh7AG324600 Rh7CG341400
rosa_wichuraiana	Rw1G015160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	3 cut(s)	63, 336, 843
Acc16I	TGCGCA	1 cut(s)	69
AccBSI	CCGCTC	1 cut(s)	754
AccI	GTMKAC	2 cut(s)	288, 324
AccII	CGCG	5 cut(s)	374, 461, 498, 611, 853
AciI	CCGC	9 cut(s)	251, 374, 496, 611, 754, 823, 851, 853, 943
AclWI	GGATC	1 cut(s)	348
AcoI	YGGCCR	1 cut(s)	222
AcsI	RAATTY	1 cut(s)	1071
AcuI	CTGAAG	1 cut(s)	68
AcvI	CACGTG	1 cut(s)	483
AcyI	GRCGYC	5 cut(s)	60, 285, 333, 509, 840
AfaI	GTAC	4 cut(s)	53, 568, 574, 758
AfiI	CCNNNNNNNGG	3 cut(s)	857, 983, 1019
AflIII	ACRYGT	3 cut(s)	459, 482, 759
AgsI	TTSAA	4 cut(s)	124, 734, 867, 967
AjnI	CCWGG	2 cut(s)	138, 240
AjuI	GAANNNNNNNTTGG	2 cut(s)	532, 564
AloI	GAACNNNNNNTCC	2 cut(s)	110, 142
AluBI	AGCT	6 cut(s)	7, 15, 505, 793, 817, 834
AluI	AGCT	6 cut(s)	7, 15, 505, 793, 817, 834
Alw26I	GTCTC	5 cut(s)	206, 760, 1014, 1113, 1128
AlwI	GGATC	1 cut(s)	348
AlwNI	CAGNNNCTG	1 cut(s)	254
Ama87I	CYCGRG	1 cut(s)	113
AoxI	GGCC	5 cut(s)	83, 135, 222, 468, 1127
ApeKI	GCWGC	6 cut(s)	70, 254, 409, 418, 642, 790
ApoI	RAATTY	1 cut(s)	1071
AspLEI	GCGC	5 cut(s)	70, 164, 209, 374, 500
AspS9I	GGNCC	3 cut(s)	83, 136, 237
AsuC2I	CCSGG	3 cut(s)	81, 293, 738
AsuHPI	GGTGA	3 cut(s)	515, 548, 1078
AsuII	TTCGAA	1 cut(s)	551
AvaI	CYCGRG	1 cut(s)	113
AvaII	GGWCC	1 cut(s)	237
BalI	TGGCCA	1 cut(s)	224
BanII	GRGCYC	2 cut(s)	248, 364
BauI	CACGAG	1 cut(s)	155
BbrPI	CACGTG	1 cut(s)	483
BbsI	GAAGAC	1 cut(s)	20
BbvI	GCAGC	6 cut(s)	82, 241, 396, 405, 654, 802
BccI	CCATC	4 cut(s)	127, 197, 450, 1028
BceAI	ACGGC	2 cut(s)	115, 186
BcgI	CGANNNNNNTGC	2 cut(s)	268, 302
BciT130I	CCWGG	2 cut(s)	140, 242
BclI	TGATCA	2 cut(s)	592, 928
BcnI	CCSGG	3 cut(s)	81, 293, 738
BcoDI	GTCTC	5 cut(s)	206, 760, 1014, 1113, 1128
BfmI	CTRYAG	1 cut(s)	877
BglII	AGATCT	1 cut(s)	677
BisI	GCNGC	9 cut(s)	71, 252, 255, 410, 419, 496, 643, 791, 854
BlpI	GCTNAGC	1 cut(s)	247
BlsI	GCNGC	9 cut(s)	72, 253, 256, 411, 420, 497, 644, 792, 855
BmcAI	AGTACT	1 cut(s)	568
Bme1390I	CCNGG	5 cut(s)	81, 140, 242, 293, 738
Bme18I	GGWCC	1 cut(s)	237
BmeT110I	CYCGRG	1 cut(s)	113
BmgT120I	GGNCC	3 cut(s)	83, 136, 237
BmiI	GGNNCC	4 cut(s)	84, 238, 239, 1000
BmrFI	CCNGG	5 cut(s)	81, 140, 242, 293, 738
BmsI	GCATC	2 cut(s)	78, 462
BpiI	GAAGAC	1 cut(s)	20
BpmI	CTGGAG	1 cut(s)	386
Bpu1102I	GCTNAGC	1 cut(s)	247
Bpu14I	TTCGAA	1 cut(s)	551
BpuEI	CTTGAG	4 cut(s)	329, 403, 803, 1095
BpuMI	CCSGG	3 cut(s)	81, 293, 738
BsaAI	YACGTR	2 cut(s)	436, 483
BsaHI	GRCGYC	5 cut(s)	60, 285, 333, 509, 840
BsaI	GGTCTC	1 cut(s)	1113
BsaJI	CCNNGG	7 cut(s)	80, 138, 240, 241, 701, 851, 953
BsaWI	WCCGGW	2 cut(s)	487, 1132
BsaXI	ACNNNNNCTCC	2 cut(s)	110, 140
Bsc4I	CCNNNNNNNGG	3 cut(s)	857, 983, 1019
Bse118I	RCCGGY	1 cut(s)	1063
Bse1I	ACTGG	1 cut(s)	574
BseBI	CCWGG	2 cut(s)	140, 242
BseDI	CCNNGG	7 cut(s)	80, 138, 240, 241, 701, 851, 953
BseGI	GGATG	1 cut(s)	442
BseLI	CCNNNNNNNGG	3 cut(s)	857, 983, 1019
BseMII	CTCAG	1 cut(s)	261
BseNI	ACTGG	1 cut(s)	574
BseRI	GAGGAG	2 cut(s)	118, 799
BseXI	GCAGC	6 cut(s)	82, 241, 396, 405, 654, 802
Bsh1236I	CGCG	5 cut(s)	374, 461, 498, 611, 853
Bsh1285I	CGRYCG	1 cut(s)	827
BshFI	GGCC	5 cut(s)	85, 137, 224, 470, 1129
BsiEI	CGRYCG	1 cut(s)	827
BsiHKCI	CYCGRG	1 cut(s)	113
BslFI	GGGAC	1 cut(s)	223
BslI	CCNNNNNNNGG	3 cut(s)	857, 983, 1019
BsmAI	GTCTC	5 cut(s)	206, 760, 1014, 1113, 1128
BsmBI	CGTCTC	1 cut(s)	206
BsmFI	GGGAC	1 cut(s)	223
BsnI	GGCC	5 cut(s)	85, 137, 224, 470, 1129
Bso31I	GGTCTC	1 cut(s)	1113
BsoBI	CYCGRG	1 cut(s)	113
Bsp119I	TTCGAA	1 cut(s)	551
Bsp1286I	GDGCHC	2 cut(s)	248, 364
Bsp143I	GATC	4 cut(s)	340, 592, 677, 928
Bsp1720I	GCTNAGC	1 cut(s)	247
BspACI	CCGC	9 cut(s)	251, 374, 496, 611, 754, 823, 851, 853, 943
BspANI	GGCC	5 cut(s)	85, 137, 224, 470, 1129
BspCNI	CTCAG	1 cut(s)	260
BspFNI	CGCG	5 cut(s)	374, 461, 498, 611, 853
BspLI	GGNNCC	4 cut(s)	84, 238, 239, 1000
BspPI	GGATC	1 cut(s)	348
BspT104I	TTCGAA	1 cut(s)	551
BspTNI	GGTCTC	1 cut(s)	1113
BsrBI	CCGCTC	1 cut(s)	754
BsrFI	RCCGGY	1 cut(s)	1063
BsrI	ACTGG	1 cut(s)	574
BssAI	RCCGGY	1 cut(s)	1063
BssECI	CCNNGG	7 cut(s)	80, 138, 240, 241, 701, 851, 953
BssMI	GATC	4 cut(s)	340, 592, 677, 928
BssNI	GRCGYC	5 cut(s)	60, 285, 333, 509, 840
BssSI	CACGAG	1 cut(s)	155
BssT1I	CCWWGG	1 cut(s)	701
Bst2BI	CACGAG	1 cut(s)	155
Bst2UI	CCWGG	2 cut(s)	140, 242
Bst4CI	ACNGT	3 cut(s)	668, 887, 978
BstACI	GRCGYC	5 cut(s)	60, 285, 333, 509, 840
BstBAI	YACGTR	2 cut(s)	436, 483
BstBI	TTCGAA	1 cut(s)	551
BstDEI	CTNAG	2 cut(s)	247, 1014
BstDSI	CCRYGG	2 cut(s)	851, 953
BstF5I	GGATG	1 cut(s)	442
BstFNI	CGCG	5 cut(s)	374, 461, 498, 611, 853
BstHHI	GCGC	5 cut(s)	70, 164, 209, 374, 500
BstKTI	GATC	4 cut(s)	343, 595, 680, 931
BstMAI	GTCTC	5 cut(s)	206, 760, 1014, 1113, 1128
BstMBI	GATC	4 cut(s)	340, 592, 677, 928
BstMCI	CGRYCG	1 cut(s)	827
BstMWI	GCNNNNNNNGC	5 cut(s)	76, 378, 415, 418, 467
BstNI	CCWGG	2 cut(s)	140, 242
BstSCI	CCNGG	5 cut(s)	79, 138, 240, 291, 736
BstSFI	CTRYAG	1 cut(s)	877
BstUI	CGCG	5 cut(s)	374, 461, 498, 611, 853
BstV1I	GCAGC	6 cut(s)	82, 241, 396, 405, 654, 802
BstV2I	GAAGAC	1 cut(s)	20
BstX2I	RGATCY	2 cut(s)	340, 677
BstYI	RGATCY	2 cut(s)	340, 677
BsuRI	GGCC	5 cut(s)	85, 137, 224, 470, 1129
BtgI	CCRYGG	2 cut(s)	851, 953
BtsCI	GGATG	1 cut(s)	442
BtsIMutI	CAGTG	2 cut(s)	54, 786
CaiI	CAGNNNCTG	1 cut(s)	254
CfoI	GCGC	5 cut(s)	70, 164, 209, 374, 500
Cfr10I	RCCGGY	1 cut(s)	1063
Cfr13I	GGNCC	3 cut(s)	83, 136, 237
Cfr42I	CCGCGG	1 cut(s)	854
CseI	GACGC	6 cut(s)	223, 274, 285, 467, 517, 617
Csp6I	GTAC	4 cut(s)	52, 567, 573, 757
CviAII	CATG	1 cut(s)	220
CviQI	GTAC	4 cut(s)	52, 567, 573, 757
DdeI	CTNAG	2 cut(s)	247, 1014
DpnI	GATC	4 cut(s)	342, 594, 679, 930
DpnII	GATC	4 cut(s)	340, 592, 677, 928
EaeI	YGGCCR	1 cut(s)	222
Eco130I	CCWWGG	1 cut(s)	701
Eco24I	GRGCYC	2 cut(s)	248, 364
Eco31I	GGTCTC	1 cut(s)	1113
Eco47I	GGWCC	1 cut(s)	237
Eco57I	CTGAAG	1 cut(s)	68
Eco72I	CACGTG	1 cut(s)	483
Eco88I	CYCGRG	1 cut(s)	113
EcoO109I	RGGNCCY	1 cut(s)	237
EcoRII	CCWGG	2 cut(s)	138, 240
EcoT14I	CCWWGG	1 cut(s)	701
EcoT38I	GRGCYC	2 cut(s)	248, 364
ErhI	CCWWGG	1 cut(s)	701
Esp3I	CGTCTC	1 cut(s)	206
FaeI	CATG	1 cut(s)	223
FaiI	YATR	4 cut(s)	12, 105, 221, 879
FaqI	GGGAC	1 cut(s)	223
FatI	CATG	1 cut(s)	219
FbaI	TGATCA	2 cut(s)	592, 928
FblI	GTMKAC	2 cut(s)	288, 324
Fnu4HI	GCNGC	9 cut(s)	71, 252, 255, 410, 419, 496, 643, 791, 854
FokI	GGATG	1 cut(s)	429
FriOI	GRGCYC	2 cut(s)	248, 364
Fsp4HI	GCNGC	9 cut(s)	71, 252, 255, 410, 419, 496, 643, 791, 854
FspI	TGCGCA	1 cut(s)	69
GlaI	GCGC	5 cut(s)	69, 163, 208, 373, 499
GluI	GCNGC	9 cut(s)	71, 252, 255, 410, 419, 496, 643, 791, 854
GsuI	CTGGAG	1 cut(s)	386
HaeIII	GGCC	5 cut(s)	85, 137, 224, 470, 1129
HgaI	GACGC	6 cut(s)	223, 274, 285, 467, 517, 617
HhaI	GCGC	5 cut(s)	70, 164, 209, 374, 500
Hin1I	GRCGYC	5 cut(s)	60, 285, 333, 509, 840
Hin1II	CATG	1 cut(s)	223
Hin6I	GCGC	5 cut(s)	68, 162, 207, 372, 498
HinP1I	GCGC	5 cut(s)	68, 162, 207, 372, 498
HincII	GTYRAC	3 cut(s)	289, 1027, 1059
HindII	GTYRAC	3 cut(s)	289, 1027, 1059
HinfI	GANTC	4 cut(s)	180, 515, 553, 776
HpaI	GTTAAC	1 cut(s)	1059
HphI	GGTGA	3 cut(s)	515, 548, 1078
Hpy166II	GTNNAC	6 cut(s)	54, 289, 325, 759, 1027, 1059
Hpy188I	TCNGA	5 cut(s)	179, 315, 331, 535, 1089
Hpy188III	TCNNGA	9 cut(s)	115, 346, 365, 446, 681, 691, 867, 926, 1112
Hpy8I	GTNNAC	6 cut(s)	54, 289, 325, 759, 1027, 1059
Hpy99I	CGWCG	9 cut(s)	179, 279, 290, 335, 461, 611, 718, 842, 845
HpyAV	CCTTC	2 cut(s)	175, 887
HpyCH4III	ACNGT	3 cut(s)	668, 887, 978
HpyCH4IV	ACGT	6 cut(s)	60, 333, 435, 482, 761, 840
HpyCH4V	TGCA	2 cut(s)	91, 453
HpyF10VI	GCNNNNNNNGC	5 cut(s)	76, 378, 415, 418, 467
HpyF3I	CTNAG	2 cut(s)	247, 1014
HpySE526I	ACGT	6 cut(s)	60, 333, 435, 482, 761, 840
Hsp92I	GRCGYC	5 cut(s)	60, 285, 333, 509, 840
Hsp92II	CATG	1 cut(s)	223
HspAI	GCGC	5 cut(s)	68, 162, 207, 372, 498
KflI	GGGWCCC	1 cut(s)	237
Ksp22I	TGATCA	2 cut(s)	592, 928
KspAI	GTTAAC	1 cut(s)	1059
KspI	CCGCGG	1 cut(s)	854
Kzo9I	GATC	4 cut(s)	340, 592, 677, 928
LmnI	GCTCC	1 cut(s)	751
Lsp1109I	GCAGC	6 cut(s)	82, 241, 396, 405, 654, 802
LweI	GCATC	2 cut(s)	78, 462
MaeII	ACGT	6 cut(s)	60, 333, 435, 482, 761, 840
MaeIII	GTNAC	2 cut(s)	705, 719
MalI	GATC	4 cut(s)	342, 594, 679, 930
MbiI	CCGCTC	1 cut(s)	754
MboI	GATC	4 cut(s)	340, 592, 677, 928
MboII	GAAGA	5 cut(s)	11, 14, 20, 160, 175
MflI	RGATCY	2 cut(s)	340, 677
MhlI	GDGCHC	2 cut(s)	248, 364
MlsI	TGGCCA	1 cut(s)	224
MluCI	AATT	4 cut(s)	547, 582, 918, 1071
MluI	ACGCGT	1 cut(s)	459
MluNI	TGGCCA	1 cut(s)	224
MlyI	GAGTC	1 cut(s)	509
MmeI	TCCRAC	2 cut(s)	157, 354
Mox20I	TGGCCA	1 cut(s)	224
MscI	TGGCCA	1 cut(s)	224
MseI	TTAA	2 cut(s)	1005, 1058
Msp20I	TGGCCA	1 cut(s)	224
MspA1I	CMGCKG	3 cut(s)	251, 793, 853
MspR9I	CCNGG	5 cut(s)	81, 140, 242, 293, 738
MvaI	CCWGG	2 cut(s)	140, 242
MvnI	CGCG	5 cut(s)	374, 461, 498, 611, 853
MwoI	GCNNNNNNNGC	5 cut(s)	76, 378, 415, 418, 467
NciI	CCSGG	3 cut(s)	81, 293, 738
NdeII	GATC	4 cut(s)	340, 592, 677, 928
NlaIII	CATG	1 cut(s)	223
NlaIV	GGNNCC	4 cut(s)	84, 238, 239, 1000
NmeAIII	GCCGAG	2 cut(s)	235, 1105
NmuCI	GTSAC	2 cut(s)	705, 719
NsbI	TGCGCA	1 cut(s)	69
NspV	TTCGAA	1 cut(s)	551
PaeR7I	CTCGAG	1 cut(s)	113
PasI	CCCWGGG	1 cut(s)	241
PcsI	WCGNNNNNNNCGW	5 cut(s)	83, 153, 327, 479, 945
PfeI	GAWTC	3 cut(s)	180, 553, 776
PkrI	GCNGC	9 cut(s)	72, 253, 256, 411, 420, 497, 644, 792, 855
PleI	GAGTC	1 cut(s)	509
PmaCI	CACGTG	1 cut(s)	483
PmlI	CACGTG	1 cut(s)	483
PpsI	GAGTC	1 cut(s)	509
Ppu21I	YACGTR	2 cut(s)	436, 483
PpuMI	RGGWCCY	1 cut(s)	237
Psp5II	RGGWCCY	1 cut(s)	237
Psp6I	CCWGG	2 cut(s)	138, 240
PspCI	CACGTG	1 cut(s)	483
PspGI	CCWGG	2 cut(s)	138, 240
PspN4I	GGNNCC	4 cut(s)	84, 238, 239, 1000
PspPI	GGNCC	3 cut(s)	83, 136, 237
PspPPI	RGGWCCY	1 cut(s)	237
PstNI	CAGNNNCTG	1 cut(s)	254
PsuI	RGATCY	2 cut(s)	340, 677
PvuII	CAGCTG	1 cut(s)	793
RsaI	GTAC	4 cut(s)	53, 568, 574, 758
RsaNI	GTAC	4 cut(s)	52, 567, 573, 757
SacII	CCGCGG	1 cut(s)	854
SalI	GTCGAC	1 cut(s)	287
SaqAI	TTAA	2 cut(s)	1005, 1058
SatI	GCNGC	9 cut(s)	71, 252, 255, 410, 419, 496, 643, 791, 854
Sau3AI	GATC	4 cut(s)	340, 592, 677, 928
Sau96I	GGNCC	3 cut(s)	83, 136, 237
ScaI	AGTACT	1 cut(s)	568
SchI	GAGTC	1 cut(s)	509
ScrFI	CCNGG	5 cut(s)	81, 140, 242, 293, 738
SduI	GDGCHC	2 cut(s)	248, 364
SfaNI	GCATC	2 cut(s)	78, 462
SfcI	CTRYAG	1 cut(s)	877
Sfr274I	CTCGAG	1 cut(s)	113
Sfr303I	CCGCGG	1 cut(s)	854
SfuI	TTCGAA	1 cut(s)	551
SgrAI	CRCCGGYG	1 cut(s)	1063
SgrBI	CCGCGG	1 cut(s)	854
SinI	GGWCC	1 cut(s)	237
SlaI	CTCGAG	1 cut(s)	113
SmlI	CTYRAG	5 cut(s)	113, 344, 382, 818, 1110
SmoI	CTYRAG	5 cut(s)	113, 344, 382, 818, 1110
Sse9I	AATT	4 cut(s)	547, 582, 918, 1071
SsiI	CCGC	9 cut(s)	251, 374, 496, 611, 754, 823, 851, 853, 943
SspI	AATATT	1 cut(s)	687
StyD4I	CCNGG	5 cut(s)	79, 138, 240, 291, 736
StyI	CCWWGG	1 cut(s)	701
TaaI	ACNGT	3 cut(s)	668, 887, 978
TaiI	ACGT	6 cut(s)	63, 336, 438, 485, 764, 843
TaqI	TCGA	9 cut(s)	114, 131, 288, 456, 551, 713, 800, 827, 948
TasI	AATT	4 cut(s)	547, 582, 918, 1071
TatI	WGTACW	2 cut(s)	51, 566
TauI	GCSGC	3 cut(s)	254, 498, 856
TfiI	GAWTC	3 cut(s)	180, 553, 776
Tru1I	TTAA	2 cut(s)	1005, 1058
Tru9I	TTAA	2 cut(s)	1005, 1058
TscAI	CASTG	2 cut(s)	61, 786
TseFI	GTSAC	2 cut(s)	705, 719
TseI	GCWGC	6 cut(s)	70, 254, 409, 418, 642, 790
Tsp45I	GTSAC	2 cut(s)	705, 719
TspDTI	ATGAA	3 cut(s)	17, 160, 594
TspGWI	ACGGA	2 cut(s)	764, 1044
TspRI	CASTG	2 cut(s)	61, 786
VpaK11BI	GGWCC	1 cut(s)	237
XapI	RAATTY	1 cut(s)	1071
XcmI	CCANNNNNNNNNTGG	1 cut(s)	566
XhoI	CTCGAG	1 cut(s)	113
XmiI	GTMKAC	2 cut(s)	288, 324
ZraI	GACGTC	3 cut(s)	61, 334, 841
ZrmI	AGTACT	1 cut(s)	568

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.