RLG00000014743

acyl-activating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001616
Physical Location & Seq
Forward (+)
57073925 .. 57074272
348 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014743

Sequence Viewer

Length: 348 bp
ATGGAATACATAGTCTCCATGCTCTCGGTTTTGAGGTGCGGTGAGGCTTTTCTGCCTTTGGACCCTTCCTGGCCAAAACAAAGGCTATTCTCCGTCATTGCTTTGGCCAATGTCGACGTAATAATCACCTGCAGGACTCCGTTTGGGTACAAATTGGACTCCAATTGGCTCGAGCAGAGTTGCAATCGAAATTCTCTGTGGTTTTCCATAGGTGGAGAGTTTGTGACAGTGAGTGATGGAGAAGAGTGTAGGCGTGAGAGTGAGAAAGAGGATGAGAGGCTGTTCTGTTATGTAATGTACAGTTCGGGCTTGACGGGGAAGCCGAAAGGTGTTTGTGGCACTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AMP-binding AMP-binding_C PP-binding PQQ PQQ_2 PQQ_3

Protein Analysis

116

Amino Acids

13.03

Weight (kDa)

4.89

Isoelectric Point (pI)

45.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AMP-binding PF00501 2 - 112 4.3e-13 AMP-binding enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 137
Acc36I ACCTGC 1 cut(s) 137
AccI GTMKAC 1 cut(s) 114
AciI CCGC 1 cut(s) 39
AcoI YGGCCR 2 cut(s) 71, 105
AcsI RAATTY 1 cut(s) 190
AfaI GTAC 2 cut(s) 149, 299
AjnI CCWGG 1 cut(s) 68
Alw26I GTCTC 1 cut(s) 19
Ama87I CYCGRG 1 cut(s) 170
AoxI GGCC 2 cut(s) 71, 105
ApoI RAATTY 1 cut(s) 190
AspS9I GGNCC 1 cut(s) 61
AsuHPI GGTGA 2 cut(s) 53, 118
AvaI CYCGRG 1 cut(s) 170
AvaII GGWCC 1 cut(s) 61
BalI TGGCCA 2 cut(s) 73, 107
BccI CCATC 1 cut(s) 230
BciT130I CCWGG 1 cut(s) 70
BcoDI GTCTC 1 cut(s) 19
BfmI CTRYAG 1 cut(s) 130
BfuAI ACCTGC 1 cut(s) 137
Bme1390I CCNGG 1 cut(s) 70
Bme18I GGWCC 1 cut(s) 61
BmeT110I CYCGRG 1 cut(s) 170
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 1 cut(s) 70
BsaXI ACNNNNNCTCC 3 cut(s) 29, 207, 237
Bse3DI GCAATG 1 cut(s) 96
BseBI CCWGG 1 cut(s) 70
BseGI GGATG 1 cut(s) 277
BseMI GCAATG 1 cut(s) 96
BshFI GGCC 2 cut(s) 73, 107
BsiHKCI CYCGRG 1 cut(s) 170
BsmAI GTCTC 1 cut(s) 19
BsnI GGCC 2 cut(s) 73, 107
BsoBI CYCGRG 1 cut(s) 170
Bsp1407I TGTACA 1 cut(s) 297
BspACI CCGC 1 cut(s) 39
BspANI GGCC 2 cut(s) 73, 107
BspLI GGNNCC 1 cut(s) 63
BspMAI CTGCAG 1 cut(s) 134
BspMI ACCTGC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 96
BsrGI TGTACA 1 cut(s) 297
Bst2UI CCWGG 1 cut(s) 70
Bst4CI ACNGT 2 cut(s) 229, 302
Bst6I CTCTTC 1 cut(s) 237
BstAUI TGTACA 1 cut(s) 297
BstF5I GGATG 1 cut(s) 277
BstMAI GTCTC 1 cut(s) 19
BstNI CCWGG 1 cut(s) 70
BstSCI CCNGG 1 cut(s) 68
BstSFI CTRYAG 1 cut(s) 130
BsuRI GGCC 2 cut(s) 73, 107
BtsCI GGATG 1 cut(s) 277
BtsIMutI CAGTG 2 cut(s) 234, 339
BveI ACCTGC 1 cut(s) 137
Cfr13I GGNCC 1 cut(s) 61
Csp6I GTAC 2 cut(s) 148, 298
CviAII CATG 1 cut(s) 19
CviJI RGCY 8 cut(s) 47, 73, 85, 107, 169, 280, 309, 322
CviKI_1 RGCY 8 cut(s) 47, 73, 85, 107, 169, 280, 309, 322
CviQI GTAC 2 cut(s) 148, 298
EaeI YGGCCR 2 cut(s) 71, 105
Eam1104I CTCTTC 1 cut(s) 237
EarI CTCTTC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 61
Eco88I CYCGRG 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 68
FaeI CATG 1 cut(s) 22
FaiI YATR 4 cut(s) 11, 20, 209, 291
FatI CATG 1 cut(s) 18
FblI GTMKAC 1 cut(s) 114
FokI GGATG 1 cut(s) 284
HaeIII GGCC 2 cut(s) 73, 107
Hin1II CATG 1 cut(s) 22
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 2 cut(s) 136, 158
HphI GGTGA 2 cut(s) 53, 118
Hpy166II GTNNAC 1 cut(s) 115
Hpy8I GTNNAC 1 cut(s) 115
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 2 cut(s) 229, 302
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 2 cut(s) 132, 183
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 1 cut(s) 22
LpnPI CCDG 4 cut(s) 55, 82, 118, 142
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 1 cut(s) 223
MboII GAAGA 1 cut(s) 254
MfeI CAATTG 1 cut(s) 163
MlsI TGGCCA 2 cut(s) 73, 107
MluCI AATT 3 cut(s) 152, 163, 190
MluNI TGGCCA 2 cut(s) 73, 107
MlyI GAGTC 2 cut(s) 130, 152
MnlI CCTC 4 cut(s) 27, 37, 262, 270
Mox20I TGGCCA 2 cut(s) 73, 107
MscI TGGCCA 2 cut(s) 73, 107
Msp20I TGGCCA 2 cut(s) 73, 107
MspR9I CCNGG 1 cut(s) 70
MunI CAATTG 1 cut(s) 163
MvaI CCWGG 1 cut(s) 70
NlaIII CATG 1 cut(s) 22
NlaIV GGNNCC 1 cut(s) 63
NmuCI GTSAC 1 cut(s) 223
PaeR7I CTCGAG 1 cut(s) 170
PaqCI CACCTGC 1 cut(s) 137
PcsI WCGNNNNNNNCGW 1 cut(s) 320
PleI GAGTC 2 cut(s) 130, 152
PpsI GAGTC 2 cut(s) 130, 152
Psp6I CCWGG 1 cut(s) 68
PspGI CCWGG 1 cut(s) 68
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 1 cut(s) 61
PspXI VCTCGAGB 1 cut(s) 170
PstI CTGCAG 1 cut(s) 134
RsaI GTAC 2 cut(s) 149, 299
RsaNI GTAC 2 cut(s) 148, 298
SalI GTCGAC 1 cut(s) 113
Sau96I GGNCC 1 cut(s) 61
SbfI CCTGCAGG 1 cut(s) 134
SchI GAGTC 2 cut(s) 130, 152
ScrFI CCNGG 1 cut(s) 70
SdaI CCTGCAGG 1 cut(s) 134
SetI ASST 5 cut(s) 38, 120, 131, 214, 331
SfcI CTRYAG 1 cut(s) 130
Sfr274I CTCGAG 1 cut(s) 170
SinI GGWCC 1 cut(s) 61
SlaI CTCGAG 1 cut(s) 170
SmlI CTYRAG 1 cut(s) 170
SmoI CTYRAG 1 cut(s) 170
Sse8387I CCTGCAGG 1 cut(s) 134
Sse9I AATT 3 cut(s) 152, 163, 190
SsiI CCGC 1 cut(s) 39
StyD4I CCNGG 1 cut(s) 68
TaaI ACNGT 2 cut(s) 229, 302
TaiI ACGT 1 cut(s) 120
TaqI TCGA 3 cut(s) 114, 171, 187
TasI AATT 3 cut(s) 152, 163, 190
TatI WGTACW 1 cut(s) 297
TscAI CASTG 2 cut(s) 234, 346
TseFI GTSAC 1 cut(s) 223
Tsp45I GTSAC 1 cut(s) 223
TspGWI ACGGA 2 cut(s) 82, 129
TspRI CASTG 2 cut(s) 234, 346
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 1 cut(s) 190
XhoI CTCGAG 1 cut(s) 170
XmiI GTMKAC 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.