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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 118 |
| AcsI | RAATTY | 1 cut(s) | 110 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 34 |
| AgsI | TTSAA | 3 cut(s) | 42, 78, 110 |
| AluBI | AGCT | 1 cut(s) | 136 |
| AluI | AGCT | 1 cut(s) | 136 |
| AlwI | GGATC | 1 cut(s) | 118 |
| ApoI | RAATTY | 1 cut(s) | 110 |
| BmsI | GCATC | 1 cut(s) | 104 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 52, 82 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 34 |
| BseGI | GGATG | 1 cut(s) | 95 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 34 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 34 |
| Bsp143I | GATC | 1 cut(s) | 123 |
| BspHI | TCATGA | 1 cut(s) | 117 |
| BspPI | GGATC | 1 cut(s) | 118 |
| BssMI | GATC | 1 cut(s) | 123 |
| BstF5I | GGATG | 1 cut(s) | 95 |
| BstKTI | GATC | 1 cut(s) | 126 |
| BstMBI | GATC | 1 cut(s) | 123 |
| BstX2I | RGATCY | 1 cut(s) | 123 |
| BstYI | RGATCY | 1 cut(s) | 123 |
| BtsCI | GGATG | 1 cut(s) | 95 |
| CciI | TCATGA | 1 cut(s) | 117 |
| CviAII | CATG | 1 cut(s) | 118 |
| CviJI | RGCY | 3 cut(s) | 17, 38, 136 |
| CviKI_1 | RGCY | 3 cut(s) | 17, 38, 136 |
| DpnI | GATC | 1 cut(s) | 125 |
| DpnII | GATC | 1 cut(s) | 123 |
| FaeI | CATG | 1 cut(s) | 121 |
| FaiI | YATR | 1 cut(s) | 119 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 125 |
| FatI | CATG | 1 cut(s) | 117 |
| FokI | GGATG | 1 cut(s) | 82 |
| Hin1II | CATG | 1 cut(s) | 121 |
| Hpy188III | TCNNGA | 1 cut(s) | 118 |
| Hsp92II | CATG | 1 cut(s) | 121 |
| Kzo9I | GATC | 1 cut(s) | 123 |
| LpnPI | CCDG | 1 cut(s) | 27 |
| LweI | GCATC | 1 cut(s) | 104 |
| MaeIII | GTNAC | 1 cut(s) | 21 |
| MalI | GATC | 1 cut(s) | 125 |
| MboI | GATC | 1 cut(s) | 123 |
| MboII | GAAGA | 1 cut(s) | 133 |
| MflI | RGATCY | 1 cut(s) | 123 |
| MluCI | AATT | 1 cut(s) | 110 |
| MmeI | TCCRAC | 1 cut(s) | 95 |
| MnlI | CCTC | 2 cut(s) | 64, 85 |
| MseI | TTAA | 1 cut(s) | 145 |
| NdeII | GATC | 1 cut(s) | 123 |
| NlaIII | CATG | 1 cut(s) | 121 |
| NmeAIII | GCCGAG | 1 cut(s) | 14 |
| NmuCI | GTSAC | 1 cut(s) | 21 |
| PagI | TCATGA | 1 cut(s) | 117 |
| PsuI | RGATCY | 1 cut(s) | 123 |
| SaqAI | TTAA | 1 cut(s) | 145 |
| Sau3AI | GATC | 1 cut(s) | 123 |
| SetI | ASST | 1 cut(s) | 138 |
| SfaNI | GCATC | 1 cut(s) | 104 |
| SgeI | CNNG | 6 cut(s) | 26, 31, 46, 101, 130, 140 |
| Sse9I | AATT | 1 cut(s) | 110 |
| TasI | AATT | 1 cut(s) | 110 |
| Tru1I | TTAA | 1 cut(s) | 145 |
| Tru9I | TTAA | 1 cut(s) | 145 |
| TseFI | GTSAC | 1 cut(s) | 21 |
| Tsp45I | GTSAC | 1 cut(s) | 21 |
| TspDTI | ATGAA | 1 cut(s) | 134 |
| TspGWI | ACGGA | 1 cut(s) | 88 |
| XapI | RAATTY | 1 cut(s) | 110 |