Rorug02G0313700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000773
Physical Location & Seq
Forward (+)
37982307 .. 37982943
637 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0313700.1

Sequence Viewer

Length: 429 bp
ATGGTTTCAGTTTCTTTATTTCCTGTCAACAAAATGAGATCTGTTGATGCAATGGCTACTAGGCTAGCCTCCTCTCTTGCTATCGCTGAAGGTAAGAGACATATCGATTTGCATCCAACACAGGGCCAAGGCTTGAGACAATCGAAGGTGGAGGAAGCTGAAGGGCAGAGAGTGTTCTTCACGTTATCTGACCCTGTAGATAGGGTTCAGATCTGGCGCGGCGCGCCATGGGGCTACAACCACTCTCCGATGGCTCTCGCCTGGTATGATGGCATCATGCCGGTGGATAAGGAGATCGATAAGAATGCTAAGAAGGCCGAGAAGCTCCGGATCAGGGTTGAGATTCCACTGAACAAGCCATTGCCGTTCAAGCGCTGGTGTTGGGTGGAAGATGAAGTCGAGTTCCTATGTAGATTCAAGTATGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

142

Amino Acids

16.43

Weight (kDa)

8.93

Isoelectric Point (pI)

53.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 219, 224
AccIII TCCGGA 1 cut(s) 327
AciI CCGC 1 cut(s) 219
AclWI GGATC 1 cut(s) 338
AcuI CTGAAG 2 cut(s) 108, 180
AfeI AGCGCT 1 cut(s) 374
AfiI CCNNNNNNNGG 2 cut(s) 122, 334
AgsI TTSAA 2 cut(s) 370, 418
AjnI CCWGG 1 cut(s) 260
AluBI AGCT 2 cut(s) 158, 325
AluI AGCT 2 cut(s) 158, 325
Alw26I GTCTC 2 cut(s) 91, 130
AlwI GGATC 1 cut(s) 338
Aor13HI TCCGGA 1 cut(s) 327
Aor51HI AGCGCT 1 cut(s) 374
AoxI GGCC 2 cut(s) 124, 315
AscI GGCGCGCC 1 cut(s) 222
AspLEI GCGC 4 cut(s) 219, 224, 226, 375
AspS9I GGNCC 1 cut(s) 124
AsuNHI GCTAGC 1 cut(s) 64
BccI CCATC 2 cut(s) 244, 263
BceAI ACGGC 1 cut(s) 349
BcgI CGANNNNNNTGC 2 cut(s) 287, 321
BciT130I CCWGG 1 cut(s) 262
BcoDI GTCTC 2 cut(s) 91, 130
BfaI CTAG 3 cut(s) 60, 65, 427
BfmI CTRYAG 1 cut(s) 195
BfoI RGCGCY 1 cut(s) 376
BglII AGATCT 2 cut(s) 38, 210
BisI GCNGC 1 cut(s) 220
BlsI GCNGC 1 cut(s) 221
Bme1390I CCNGG 1 cut(s) 262
BmgT120I GGNCC 1 cut(s) 124
BmrFI CCNGG 1 cut(s) 262
BmsI GCATC 3 cut(s) 37, 121, 282
BmtI GCTAGC 1 cut(s) 68
BpuEI CTTGAG 1 cut(s) 154
Bsa29I ATCGAT 2 cut(s) 105, 297
BsaBI GATNNNNATC 1 cut(s) 111
BsaJI CCNNGG 2 cut(s) 127, 227
BsaWI WCCGGW 1 cut(s) 327
Bsc4I CCNNNNNNNGG 2 cut(s) 122, 334
Bse118I RCCGGY 1 cut(s) 280
Bse3DI GCAATG 2 cut(s) 57, 359
Bse8I GATNNNNATC 1 cut(s) 111
BseAI TCCGGA 1 cut(s) 327
BseBI CCWGG 1 cut(s) 262
BseCI ATCGAT 2 cut(s) 105, 297
BseDI CCNNGG 2 cut(s) 127, 227
BseGI GGATG 1 cut(s) 112
BseJI GATNNNNATC 1 cut(s) 111
BseLI CCNNNNNNNGG 2 cut(s) 122, 334
BseMI GCAATG 2 cut(s) 57, 359
BsePI GCGCGC 1 cut(s) 222
BseRI GAGGAG 1 cut(s) 61
Bsh1236I CGCG 2 cut(s) 219, 224
BshFI GGCC 2 cut(s) 126, 317
BshVI ATCGAT 2 cut(s) 105, 297
BsiSI CCGG 2 cut(s) 281, 328
BslI CCNNNNNNNGG 2 cut(s) 122, 334
BsmAI GTCTC 2 cut(s) 91, 130
BsmI GAATGC 1 cut(s) 310
BsnI GGCC 2 cut(s) 126, 317
Bsp13I TCCGGA 1 cut(s) 327
Bsp143I GATC 4 cut(s) 38, 210, 294, 330
Bsp19I CCATGG 1 cut(s) 227
BspACI CCGC 1 cut(s) 219
BspANI GGCC 2 cut(s) 126, 317
BspDI ATCGAT 2 cut(s) 105, 297
BspEI TCCGGA 1 cut(s) 327
BspFNI CGCG 2 cut(s) 219, 224
BspOI GCTAGC 1 cut(s) 68
BspPI GGATC 1 cut(s) 338
BsrDI GCAATG 2 cut(s) 57, 359
BsrFI RCCGGY 1 cut(s) 280
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 2 cut(s) 127, 227
BssHII GCGCGC 1 cut(s) 222
BssMI GATC 4 cut(s) 38, 210, 294, 330
BssT1I CCWWGG 2 cut(s) 127, 227
Bst2UI CCWGG 1 cut(s) 262
BstC8I GCNNGC 2 cut(s) 66, 224
BstDEI CTNAG 1 cut(s) 309
BstDSI CCRYGG 1 cut(s) 227
BstF5I GGATG 1 cut(s) 112
BstFNI CGCG 2 cut(s) 219, 224
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 4 cut(s) 219, 224, 226, 375
BstKTI GATC 4 cut(s) 41, 213, 297, 333
BstMAI GTCTC 2 cut(s) 91, 130
BstMBI GATC 4 cut(s) 38, 210, 294, 330
BstMWI GCNNNNNNNGC 3 cut(s) 223, 314, 370
BstNI CCWGG 1 cut(s) 262
BstSCI CCNGG 1 cut(s) 260
BstSFI CTRYAG 1 cut(s) 195
BstUI CGCG 2 cut(s) 219, 224
BstX2I RGATCY 2 cut(s) 38, 210
BstYI RGATCY 2 cut(s) 38, 210
Bsu15I ATCGAT 2 cut(s) 105, 297
BsuRI GGCC 2 cut(s) 126, 317
BsuTUI ATCGAT 2 cut(s) 105, 297
BtgI CCRYGG 1 cut(s) 227
BtsCI GGATG 1 cut(s) 112
BtsIMutI CAGTG 1 cut(s) 347
Cac8I GCNNGC 2 cut(s) 66, 224
CfoI GCGC 4 cut(s) 219, 224, 226, 375
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 1 cut(s) 124
ClaI ATCGAT 2 cut(s) 105, 297
CviAII CATG 2 cut(s) 228, 277
DdeI CTNAG 1 cut(s) 309
DpnI GATC 4 cut(s) 40, 212, 296, 332
DpnII GATC 4 cut(s) 38, 210, 294, 330
Eco130I CCWWGG 2 cut(s) 127, 227
Eco47III AGCGCT 1 cut(s) 374
Eco57I CTGAAG 2 cut(s) 108, 180
EcoRII CCWGG 1 cut(s) 260
EcoT14I CCWWGG 2 cut(s) 127, 227
ErhI CCWWGG 2 cut(s) 127, 227
FaeI CATG 2 cut(s) 231, 280
FaiI YATR 6 cut(s) 102, 229, 267, 278, 409, 423
FatI CATG 2 cut(s) 227, 276
Fnu4HI GCNGC 1 cut(s) 220
FokI GGATG 1 cut(s) 99
Fsp4HI GCNGC 1 cut(s) 220
FspBI CTAG 3 cut(s) 60, 65, 427
GlaI GCGC 4 cut(s) 218, 223, 225, 374
GluI GCNGC 1 cut(s) 220
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 2 cut(s) 126, 317
HapII CCGG 2 cut(s) 281, 328
HhaI GCGC 4 cut(s) 219, 224, 226, 375
Hin1II CATG 2 cut(s) 231, 280
Hin6I GCGC 4 cut(s) 217, 222, 224, 373
HinP1I GCGC 4 cut(s) 217, 222, 224, 373
HincII GTYRAC 1 cut(s) 28
HindII GTYRAC 1 cut(s) 28
HinfI GANTC 2 cut(s) 343, 414
HpaII CCGG 2 cut(s) 281, 328
Hpy166II GTNNAC 1 cut(s) 28
Hpy188I TCNGA 3 cut(s) 190, 210, 249
Hpy188III TCNNGA 1 cut(s) 328
Hpy8I GTNNAC 1 cut(s) 28
HpyAV CCTTC 4 cut(s) 83, 139, 155, 307
HpyCH4IV ACGT 1 cut(s) 182
HpyCH4V TGCA 2 cut(s) 50, 112
HpyF10VI GCNNNNNNNGC 3 cut(s) 223, 314, 370
HpyF3I CTNAG 1 cut(s) 309
HpySE526I ACGT 1 cut(s) 182
Hsp92II CATG 2 cut(s) 231, 280
HspAI GCGC 4 cut(s) 217, 222, 224, 373
Kpn2I TCCGGA 1 cut(s) 327
Kzo9I GATC 4 cut(s) 38, 210, 294, 330
LmnI GCTCC 1 cut(s) 330
LweI GCATC 3 cut(s) 37, 121, 282
MaeI CTAG 3 cut(s) 60, 65, 427
MaeII ACGT 1 cut(s) 182
MalI GATC 4 cut(s) 40, 212, 296, 332
MboI GATC 4 cut(s) 38, 210, 294, 330
MboII GAAGA 2 cut(s) 169, 401
MflI RGATCY 2 cut(s) 38, 210
MmeI TCCRAC 1 cut(s) 140
MnlI CCTC 3 cut(s) 79, 82, 145
MroI TCCGGA 1 cut(s) 327
MslI CAYNNNNRTG 1 cut(s) 281
MspI CCGG 2 cut(s) 281, 328
MspR9I CCNGG 1 cut(s) 262
MteI GCGCNGCGC 1 cut(s) 220
Mva1269I GAATGC 1 cut(s) 310
MvaI CCWGG 1 cut(s) 262
MvnI CGCG 2 cut(s) 219, 224
MwoI GCNNNNNNNGC 3 cut(s) 223, 314, 370
NcoI CCATGG 1 cut(s) 227
NdeII GATC 4 cut(s) 38, 210, 294, 330
NheI GCTAGC 1 cut(s) 64
NlaIII CATG 2 cut(s) 231, 280
NmeAIII GCCGAG 1 cut(s) 343
PalAI GGCGCGCC 1 cut(s) 222
PauI GCGCGC 1 cut(s) 222
PctI GAATGC 1 cut(s) 310
PfeI GAWTC 2 cut(s) 343, 414
PkrI GCNGC 1 cut(s) 221
Psp6I CCWGG 1 cut(s) 260
PspGI CCWGG 1 cut(s) 260
PspPI GGNCC 1 cut(s) 124
PsrI GAACNNNNNNTAC 2 cut(s) 189, 221
PsuI RGATCY 2 cut(s) 38, 210
PteI GCGCGC 1 cut(s) 222
RseI CAYNNNNRTG 1 cut(s) 281
SatI GCNGC 1 cut(s) 220
Sau3AI GATC 4 cut(s) 38, 210, 294, 330
Sau96I GGNCC 1 cut(s) 124
ScrFI CCNGG 1 cut(s) 262
SetI ASST 5 cut(s) 94, 150, 160, 185, 327
SfaNI GCATC 3 cut(s) 37, 121, 282
SfcI CTRYAG 1 cut(s) 195
SgsI GGCGCGCC 1 cut(s) 222
SmiMI CAYNNNNRTG 1 cut(s) 281
SmlI CTYRAG 1 cut(s) 133
SmoI CTYRAG 1 cut(s) 133
SsiI CCGC 1 cut(s) 219
SspMI CTAG 3 cut(s) 60, 65, 427
StyD4I CCNGG 1 cut(s) 260
StyI CCWWGG 2 cut(s) 127, 227
TaiI ACGT 1 cut(s) 185
TaqI TCGA 4 cut(s) 105, 143, 297, 399
TauI GCSGC 1 cut(s) 222
TfiI GAWTC 2 cut(s) 343, 414
TscAI CASTG 1 cut(s) 354
TspDTI ATGAA 1 cut(s) 408
TspRI CASTG 1 cut(s) 354
XspI CTAG 3 cut(s) 60, 65, 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.