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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 2 cut(s) | 73, 175 |
| AgsI | TTSAA | 3 cut(s) | 35, 52, 182 |
| AluBI | AGCT | 2 cut(s) | 80, 167 |
| AluI | AGCT | 2 cut(s) | 80, 167 |
| ApoI | RAATTY | 2 cut(s) | 73, 175 |
| AspS9I | GGNCC | 1 cut(s) | 127 |
| AsuHPI | GGTGA | 1 cut(s) | 82 |
| AvaII | GGWCC | 1 cut(s) | 127 |
| BccI | CCATC | 1 cut(s) | 10 |
| Bme18I | GGWCC | 1 cut(s) | 127 |
| BmgT120I | GGNCC | 1 cut(s) | 127 |
| BseMII | CTCAG | 1 cut(s) | 171 |
| BseRI | GAGGAG | 1 cut(s) | 22 |
| BspCNI | CTCAG | 1 cut(s) | 170 |
| BstC8I | GCNNGC | 1 cut(s) | 82 |
| BstDEI | CTNAG | 1 cut(s) | 157 |
| BtsIMutI | CAGTG | 2 cut(s) | 100, 215 |
| Cac8I | GCNNGC | 1 cut(s) | 82 |
| Cfr13I | GGNCC | 1 cut(s) | 127 |
| CviJI | RGCY | 3 cut(s) | 13, 80, 167 |
| CviKI_1 | RGCY | 3 cut(s) | 13, 80, 167 |
| DdeI | CTNAG | 1 cut(s) | 157 |
| Eco47I | GGWCC | 1 cut(s) | 127 |
| EcoRI | GAATTC | 1 cut(s) | 73 |
| FaiI | YATR | 1 cut(s) | 172 |
| HinfI | GANTC | 1 cut(s) | 90 |
| HphI | GGTGA | 1 cut(s) | 82 |
| Hpy188I | TCNGA | 2 cut(s) | 131, 160 |
| Hpy188III | TCNNGA | 1 cut(s) | 189 |
| Hpy99I | CGWCG | 1 cut(s) | 135 |
| HpyF3I | CTNAG | 1 cut(s) | 157 |
| LpnPI | CCDG | 1 cut(s) | 84 |
| MboII | GAAGA | 1 cut(s) | 16 |
| MluCI | AATT | 4 cut(s) | 35, 73, 175, 182 |
| MlyI | GAGTC | 1 cut(s) | 99 |
| MmeI | TCCRAC | 1 cut(s) | 154 |
| MnlI | CCTC | 1 cut(s) | 3 |
| MseI | TTAA | 1 cut(s) | 216 |
| PleI | GAGTC | 1 cut(s) | 98 |
| PpsI | GAGTC | 1 cut(s) | 98 |
| PspPI | GGNCC | 1 cut(s) | 127 |
| SaqAI | TTAA | 1 cut(s) | 216 |
| Sau96I | GGNCC | 1 cut(s) | 127 |
| SchI | GAGTC | 1 cut(s) | 99 |
| SetI | ASST | 3 cut(s) | 82, 169, 196 |
| SgeI | CNNG | 5 cut(s) | 93, 111, 146, 201, 215 |
| SinI | GGWCC | 1 cut(s) | 127 |
| Sse9I | AATT | 4 cut(s) | 35, 73, 175, 182 |
| TaqI | TCGA | 1 cut(s) | 190 |
| TasI | AATT | 4 cut(s) | 35, 73, 175, 182 |
| Tru1I | TTAA | 1 cut(s) | 216 |
| Tru9I | TTAA | 1 cut(s) | 216 |
| TscAI | CASTG | 2 cut(s) | 100, 215 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspRI | CASTG | 2 cut(s) | 100, 215 |
| VpaK11BI | GGWCC | 1 cut(s) | 127 |
| XapI | RAATTY | 2 cut(s) | 73, 175 |