Rorug02G0266300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002050
Physical Location & Seq
Reverse (-)
28163381 .. 28166659
3279 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0266300.1

Sequence Viewer

Length: 1548 bp
ATGATGGGGACTTCAACACTAGTTTGCGCTCCGATAATGGCGGAAACGGTTCGCAAGATGGTGAGTGATATGGGCAAGGCAACAGCCCTTGGTGCTGATCTAGTGGAGATTAGATTGGATTATTTGAAGGTCTTCAATAGCAATGAAGATCTTAAGACCCTAATTAAAGAATCCCCTTTGCCAACTCTTTTCACTTATAGACCAAAGTGGCAAGGTGGTCAGTATGATGGTGATGAAAAACATCGACTGGATGCTCTTCGATTAGCGATGGAGCTTGGAGCTGATTACGTTGATGTCGAGTTTCAGGTTGCTCATGAATTCATCGACTCCATATATGGAAAGAAGCCTGACAAGTTTAAAGCTATTGTTTCTTCTCACAACTATCAAGATACTCCATCTGTCGAGGATCTCAGAGATCTTGTTGCTAGAATGCAAGCCACTGGAGCTGACATAGTCAGCATCACAACCACTGCCTTGGATATTACTGACGTAGCACGCATTTTTCAGATAACTGTGCATTCTCAAGTCCCAGTAATAGGACTTGCAATGGGTGAGAGGGGCTTGATTTCTCGGATACTCTGCGCAAAATTTGGTGGTTACCTTACTTCTGGTACACTCGATTCAGGAATTGTTTCAGTTCCTGGAGAACCTGCCATCAAGGATATATTACATGTATACAACTTCAGACTGATAAGACCTGGTACAAAACTGTTTGGTGTTATTGGAAAGCCTATCAGCCACAGCAAATCTCCTCTTCTCTACAATGAAGGATTCAAGTCAGTAGGTTTCGATGGAGTTTATCTACATCTTTTGGTAGATGACATTGCAAATTTTCTCCGTACTTACTCATCAACTGACTTTTCTGGATTCAGCGTTGGAATTCCTCACAAGGAAGCTGCAGTTAAGTGCTGTGATGAGGTAGATCCAGTTGCCAAGTCAATAGGAGCTGTTAACTGCATTGTAAGGAGGCCAACTGATGGGAAGTTATTTGGCTTAAATACTGACTATTTTGGGTCTATTTCTGCTATCGAGGATGGACTTCGAGGTTCACATGATAGAGGCAATACCACTGGTTCACCTTTAGCTGGTAGGTTGTTTGTTGTCATGGGTGCCGGAGGTGCTGGAAAGGCGCTTGCTTATGGTGCAAAGCAGAAGGGAGCTAGAATTGTTATTATTGCCGACCGCGAATATGATCGAGCCAGAAAACTTGCAGACGAAGTTGGAGGAGATGCTTTGTCTTTTGCTGAATTAGAAAATTTCCACCCAGAGGACGGCACGGTTCTTGCAAATGCAACACCTGTCGGAATGCAACCGAATATTGATGAGACACCTATTCCTAAGCATGCTCTGAGATCATATTCGTTGGTGTTTGATGCCGTTTACAACCCCAAAATGACTAGACTTTTGAGCGAAGCTGCGGAGTCCGGTGTCACAGTTGTTAGTGGGGTTGAGATGCTCATTGGACAGGCATATGAACAGTTTGAGAGGTTCACCGGATTGCCTGCACCAAAGGAACTGTTTAGGAAAGTTATGGAAAATTGCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

515

Amino Acids

55.96

Weight (kDa)

5.74

Isoelectric Point (pI)

21.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHquinase_I PF01487 9 - 226 5.5e-63 Type I 3-dehydroquinase
Shikimate_dh_N PF08501 240 - 320 6.2e-25 Shikimate dehydrogenase substrate binding domain
Shikimate_DH PF01488 362 - 419 9.9e-06 Shikimate / quinate 5-dehydrogenase
SDH_C PF18317 482 - 511 3.4e-10 Shikimate 5'-dehydrogenase C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 583
Acc36I ACCTGC 1 cut(s) 658
AccB1I GGYRCC 1 cut(s) 1109
AccB7I CCANNNNNTGG 1 cut(s) 977
AccI GTMKAC 1 cut(s) 675
AccII CGCG 1 cut(s) 1185
AciI CCGC 3 cut(s) 41, 1183, 1418
AclWI GGATC 2 cut(s) 414, 917
AcsI RAATTY 5 cut(s) 317, 587, 829, 879, 1255
AcuI CTGAAG 1 cut(s) 667
AfaI GTAC 3 cut(s) 613, 703, 841
AfiI CCNNNNNNNGG 5 cut(s) 536, 977, 1085, 1267, 1271
AflII CTTAAG 1 cut(s) 152
AflIII ACRYGT 1 cut(s) 670
AgsI TTSAA 4 cut(s) 15, 127, 136, 775
AhlI ACTAGT 1 cut(s) 19
AjnI CCWGG 2 cut(s) 640, 697
AluBI AGCT 9 cut(s) 274, 281, 362, 446, 896, 947, 1085, 1160, 1415
AluI AGCT 9 cut(s) 274, 281, 362, 446, 896, 947, 1085, 1160, 1415
Alw26I GTCTC 1 cut(s) 1319
AlwI GGATC 2 cut(s) 414, 917
AlwNI CAGNNNCTG 1 cut(s) 641
AoxI GGCC 1 cut(s) 968
ApeKI GCWGC 2 cut(s) 896, 1415
ApoI RAATTY 5 cut(s) 317, 587, 829, 879, 1255
ArsI GACNNNNNNTTYG 2 cut(s) 1387, 1419
Asp700I GAANNNNTTC 3 cut(s) 48, 131, 631
AspLEI GCGC 3 cut(s) 29, 584, 1132
AsuHPI GGTGA 5 cut(s) 73, 242, 563, 1068, 1483
BanI GGYRCC 1 cut(s) 1109
BbsI GAAGAC 1 cut(s) 124
BbvI GCAGC 2 cut(s) 883, 1402
BccI CCATC 8 cut(s) 52, 221, 262, 403, 662, 785, 971, 1028
BceAI ACGGC 2 cut(s) 1288, 1361
BciT130I CCWGG 2 cut(s) 642, 699
BciVI GTATCC 1 cut(s) 567
BcoDI GTCTC 1 cut(s) 1319
BcuI ACTAGT 1 cut(s) 19
BfaI CTAG 5 cut(s) 20, 101, 426, 1161, 1398
BfmI CTRYAG 1 cut(s) 897
BfoI RGCGCY 1 cut(s) 1133
BfrI CTTAAG 1 cut(s) 152
BfuAI ACCTGC 1 cut(s) 658
BfuI GTATCC 1 cut(s) 567
BglII AGATCT 2 cut(s) 148, 415
BisI GCNGC 2 cut(s) 897, 1416
BlsI GCNGC 2 cut(s) 898, 1417
Bme1390I CCNGG 2 cut(s) 642, 699
BmiI GGNNCC 1 cut(s) 1111
BmrFI CCNGG 2 cut(s) 642, 699
BmrI ACTGGG 1 cut(s) 524
BmsI GCATC 5 cut(s) 241, 468, 1219, 1363, 1443
BmuI ACTGGG 1 cut(s) 524
BpiI GAAGAC 1 cut(s) 124
BpmI CTGGAG 2 cut(s) 462, 663
Bpu10I CCTNAGC 1 cut(s) 1338
BpuEI CTTGAG 1 cut(s) 507
BsaJI CCNNGG 2 cut(s) 88, 474
BsaWI WCCGGW 2 cut(s) 1424, 1493
Bsc4I CCNNNNNNNGG 5 cut(s) 536, 977, 1085, 1267, 1271
Bse1I ACTGG 5 cut(s) 252, 445, 530, 926, 1075
Bse3DI GCAATG 3 cut(s) 148, 552, 822
BseBI CCWGG 2 cut(s) 642, 699
BseDI CCNNGG 2 cut(s) 88, 474
BseGI GGATG 2 cut(s) 256, 1039
BseLI CCNNNNNNNGG 5 cut(s) 536, 977, 1085, 1267, 1271
BseMI GCAATG 3 cut(s) 148, 552, 822
BseMII CTCAG 2 cut(s) 424, 1340
BseNI ACTGG 5 cut(s) 252, 445, 530, 926, 1075
BseRI GAGGAG 2 cut(s) 741, 1239
BseXI GCAGC 2 cut(s) 883, 1402
BsgI GTGCAG 1 cut(s) 1488
Bsh1236I CGCG 1 cut(s) 1185
Bsh1285I CGRYCG 1 cut(s) 1183
BshFI GGCC 1 cut(s) 970
BshNI GGYRCC 1 cut(s) 1109
BsiEI CGRYCG 1 cut(s) 1183
BsiSI CCGG 3 cut(s) 1113, 1425, 1494
BslFI GGGAC 2 cut(s) 22, 512
BslI CCNNNNNNNGG 5 cut(s) 536, 977, 1085, 1267, 1271
BsmAI GTCTC 1 cut(s) 1319
BsmFI GGGAC 2 cut(s) 22, 512
BsmI GAATGC 3 cut(s) 435, 517, 1311
BsnI GGCC 1 cut(s) 970
Bsp143I GATC 7 cut(s) 97, 148, 406, 415, 922, 1192, 1352
BspACI CCGC 3 cut(s) 41, 1183, 1418
BspANI GGCC 1 cut(s) 970
BspCNI CTCAG 2 cut(s) 423, 1341
BspFNI CGCG 1 cut(s) 1185
BspHI TCATGA 1 cut(s) 313
BspLI GGNNCC 1 cut(s) 1111
BspMAI CTGCAG 1 cut(s) 901
BspMI ACCTGC 1 cut(s) 658
BspPI GGATC 2 cut(s) 414, 917
BspQI GCTCTTC 1 cut(s) 261
BspT107I GGYRCC 1 cut(s) 1109
BspTI CTTAAG 1 cut(s) 152
BsrDI GCAATG 3 cut(s) 148, 552, 822
BsrI ACTGG 5 cut(s) 252, 445, 530, 926, 1075
BssECI CCNNGG 2 cut(s) 88, 474
BssMI GATC 7 cut(s) 97, 148, 406, 415, 922, 1192, 1352
BssNAI GTATAC 1 cut(s) 676
BssT1I CCWWGG 2 cut(s) 88, 474
Bst1107I GTATAC 1 cut(s) 676
Bst2UI CCWGG 2 cut(s) 642, 699
Bst4CI ACNGT 7 cut(s) 49, 514, 711, 1279, 1435, 1479, 1518
Bst6I CTCTTC 2 cut(s) 261, 759
BstAFI CTTAAG 1 cut(s) 152
BstC8I GCNNGC 5 cut(s) 435, 496, 1134, 1344, 1503
BstDEI CTNAG 3 cut(s) 410, 1338, 1349
BstEII GGTNACC 1 cut(s) 596
BstF5I GGATG 2 cut(s) 256, 1039
BstFNI CGCG 1 cut(s) 1185
BstH2I RGCGCY 1 cut(s) 1133
BstHHI GCGC 3 cut(s) 29, 584, 1132
BstKTI GATC 7 cut(s) 100, 151, 409, 418, 925, 1195, 1355
BstMAI GTCTC 1 cut(s) 1319
BstMBI GATC 7 cut(s) 97, 148, 406, 415, 922, 1192, 1352
BstMCI CGRYCG 1 cut(s) 1183
BstMWI GCNNNNNNNGC 5 cut(s) 92, 443, 1118, 1127, 1142
BstNI CCWGG 2 cut(s) 642, 699
BstNSI RCATGY 2 cut(s) 674, 1346
BstPI GGTNACC 1 cut(s) 596
BstSCI CCNGG 2 cut(s) 640, 697
BstSFI CTRYAG 1 cut(s) 897
BstUI CGCG 1 cut(s) 1185
BstV1I GCAGC 2 cut(s) 883, 1402
BstV2I GAAGAC 1 cut(s) 124
BstX2I RGATCY 4 cut(s) 148, 406, 415, 922
BstXI CCANNNNNNTGG 1 cut(s) 475
BstYI RGATCY 4 cut(s) 148, 406, 415, 922
BstZ17I GTATAC 1 cut(s) 676
BsuI GTATCC 1 cut(s) 567
BsuRI GGCC 1 cut(s) 970
BtgZI GCGATG 1 cut(s) 281
BtsCI GGATG 2 cut(s) 256, 1039
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 3 cut(s) 438, 468, 1068
BveI ACCTGC 1 cut(s) 658
Cac8I GCNNGC 5 cut(s) 435, 496, 1134, 1344, 1503
CaiI CAGNNNCTG 1 cut(s) 641
CciI TCATGA 1 cut(s) 313
CfoI GCGC 3 cut(s) 29, 584, 1132
CsiI ACCWGGT 1 cut(s) 697
Csp6I GTAC 3 cut(s) 612, 702, 840
CviAII CATG 5 cut(s) 314, 671, 1052, 1105, 1343
CviQI GTAC 3 cut(s) 612, 702, 840
DdeI CTNAG 3 cut(s) 410, 1338, 1349
DpnI GATC 7 cut(s) 99, 150, 408, 417, 924, 1194, 1354
DpnII GATC 7 cut(s) 97, 148, 406, 415, 922, 1192, 1352
DraI TTTAAA 1 cut(s) 358
Eam1104I CTCTTC 2 cut(s) 261, 759
EarI CTCTTC 2 cut(s) 261, 759
EciI GGCGGA 1 cut(s) 56
Eco130I CCWWGG 2 cut(s) 88, 474
Eco57I CTGAAG 1 cut(s) 667
Eco91I GGTNACC 1 cut(s) 596
EcoO65I GGTNACC 1 cut(s) 596
EcoRI GAATTC 2 cut(s) 317, 879
EcoRII CCWGG 2 cut(s) 640, 697
EcoT14I CCWWGG 2 cut(s) 88, 474
ErhI CCWWGG 2 cut(s) 88, 474
FaeI CATG 5 cut(s) 317, 674, 1055, 1108, 1346
FaqI GGGAC 2 cut(s) 22, 512
FatI CATG 5 cut(s) 313, 670, 1051, 1104, 1342
FauNDI CATATG 1 cut(s) 1471
FblI GTMKAC 1 cut(s) 675
Fnu4HI GCNGC 2 cut(s) 897, 1416
FokI GGATG 2 cut(s) 263, 1046
Fsp4HI GCNGC 2 cut(s) 897, 1416
FspBI CTAG 5 cut(s) 20, 101, 426, 1161, 1398
FspI TGCGCA 1 cut(s) 583
GlaI GCGC 3 cut(s) 28, 583, 1131
GluI GCNGC 2 cut(s) 897, 1416
GsuI CTGGAG 2 cut(s) 462, 663
HaeII RGCGCY 1 cut(s) 1133
HaeIII GGCC 1 cut(s) 970
HapII CCGG 3 cut(s) 1113, 1425, 1494
HhaI GCGC 3 cut(s) 29, 584, 1132
Hin1II CATG 5 cut(s) 317, 674, 1055, 1108, 1346
Hin6I GCGC 3 cut(s) 27, 582, 1130
HinP1I GCGC 3 cut(s) 27, 582, 1130
HincII GTYRAC 1 cut(s) 952
HindII GTYRAC 1 cut(s) 952
HinfI GANTC 6 cut(s) 170, 326, 620, 771, 867, 1421
HpaI GTTAAC 1 cut(s) 952
HpaII CCGG 3 cut(s) 1113, 1425, 1494
HphI GGTGA 5 cut(s) 73, 242, 563, 1068, 1483
Hpy166II GTNNAC 7 cut(s) 614, 676, 952, 1049, 1076, 1381, 1491
Hpy188I TCNGA 7 cut(s) 33, 413, 507, 573, 686, 1304, 1350
Hpy188III TCNNGA 4 cut(s) 314, 386, 624, 864
Hpy8I GTNNAC 7 cut(s) 614, 676, 952, 1049, 1076, 1381, 1491
HpyAV CCTTC 3 cut(s) 121, 761, 1147
HpyCH4III ACNGT 7 cut(s) 49, 514, 711, 1279, 1435, 1479, 1518
HpyCH4IV ACGT 2 cut(s) 288, 489
HpyF10VI GCNNNNNNNGC 5 cut(s) 92, 443, 1118, 1127, 1142
HpyF3I CTNAG 3 cut(s) 410, 1338, 1349
HpySE526I ACGT 2 cut(s) 288, 489
Hsp92II CATG 5 cut(s) 317, 674, 1055, 1108, 1346
HspAI GCGC 3 cut(s) 27, 582, 1130
KspAI GTTAAC 1 cut(s) 952
Kzo9I GATC 7 cut(s) 97, 148, 406, 415, 922, 1192, 1352
LguI GCTCTTC 1 cut(s) 261
LmnI GCTCC 6 cut(s) 34, 271, 278, 443, 944, 1157
Lsp1109I GCAGC 2 cut(s) 883, 1402
LweI GCATC 5 cut(s) 241, 468, 1219, 1363, 1443
MabI ACCWGGT 1 cut(s) 697
MaeI CTAG 5 cut(s) 20, 101, 426, 1161, 1398
MaeII ACGT 2 cut(s) 288, 489
MaeIII GTNAC 2 cut(s) 596, 1429
MalI GATC 7 cut(s) 99, 150, 408, 417, 924, 1194, 1354
MboI GATC 7 cut(s) 97, 148, 406, 415, 922, 1192, 1352
MboII GAAGA 5 cut(s) 124, 158, 248, 363, 746
MflI RGATCY 4 cut(s) 148, 406, 415, 922
MlyI GAGTC 2 cut(s) 320, 1430
MmeI TCCRAC 3 cut(s) 856, 1201, 1282
MroXI GAANNNNTTC 3 cut(s) 48, 131, 631
MseI TTAA 6 cut(s) 153, 165, 357, 903, 951, 995
MspCI CTTAAG 1 cut(s) 152
MspI CCGG 3 cut(s) 1113, 1425, 1494
MspR9I CCNGG 2 cut(s) 642, 699
Mva1269I GAATGC 3 cut(s) 435, 517, 1311
MvaI CCWGG 2 cut(s) 642, 699
MvnI CGCG 1 cut(s) 1185
MwoI GCNNNNNNNGC 5 cut(s) 92, 443, 1118, 1127, 1142
NdeI CATATG 1 cut(s) 1471
NdeII GATC 7 cut(s) 97, 148, 406, 415, 922, 1192, 1352
NlaIII CATG 5 cut(s) 317, 674, 1055, 1108, 1346
NlaIV GGNNCC 1 cut(s) 1111
NmuCI GTSAC 1 cut(s) 1429
NsbI TGCGCA 1 cut(s) 583
NspI RCATGY 2 cut(s) 674, 1346
PaeI GCATGC 1 cut(s) 1346
PagI TCATGA 1 cut(s) 313
PciI ACATGT 1 cut(s) 670
PciSI GCTCTTC 1 cut(s) 261
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PctI GAATGC 3 cut(s) 435, 517, 1311
PdmI GAANNNNTTC 3 cut(s) 48, 131, 631
PfeI GAWTC 4 cut(s) 170, 620, 771, 867
PflFI GACNNNGTC 1 cut(s) 452
PflMI CCANNNNNTGG 1 cut(s) 977
PfoI TCCNGGA 1 cut(s) 640
PkrI GCNGC 2 cut(s) 898, 1417
PleI GAGTC 2 cut(s) 320, 1429
PpsI GAGTC 2 cut(s) 320, 1429
PscI ACATGT 1 cut(s) 670
Psp6I CCWGG 2 cut(s) 640, 697
PspEI GGTNACC 1 cut(s) 596
PspGI CCWGG 2 cut(s) 640, 697
PspN4I GGNNCC 1 cut(s) 1111
PstI CTGCAG 1 cut(s) 901
PstNI CAGNNNCTG 1 cut(s) 641
PsuI RGATCY 4 cut(s) 148, 406, 415, 922
PsyI GACNNNGTC 1 cut(s) 452
RsaI GTAC 3 cut(s) 613, 703, 841
RsaNI GTAC 3 cut(s) 612, 702, 840
SapI GCTCTTC 1 cut(s) 261
SaqAI TTAA 6 cut(s) 153, 165, 357, 903, 951, 995
SatI GCNGC 2 cut(s) 897, 1416
Sau3AI GATC 7 cut(s) 97, 148, 406, 415, 922, 1192, 1352
SchI GAGTC 2 cut(s) 320, 1430
ScrFI CCNGG 2 cut(s) 642, 699
SexAI ACCWGGT 1 cut(s) 697
SfaNI GCATC 5 cut(s) 241, 468, 1219, 1363, 1443
SfcI CTRYAG 1 cut(s) 897
SmlI CTYRAG 2 cut(s) 152, 522
SmoI CTYRAG 2 cut(s) 152, 522
SpeI ACTAGT 1 cut(s) 19
SphI GCATGC 1 cut(s) 1346
SsiI CCGC 3 cut(s) 41, 1183, 1418
SspI AATATT 1 cut(s) 1318
SspMI CTAG 5 cut(s) 20, 101, 426, 1161, 1398
StyD4I CCNGG 2 cut(s) 640, 697
StyI CCWWGG 2 cut(s) 88, 474
TaaI ACNGT 7 cut(s) 49, 514, 711, 1279, 1435, 1479, 1518
TaiI ACGT 2 cut(s) 291, 492
TfiI GAWTC 4 cut(s) 170, 620, 771, 867
Tru1I TTAA 6 cut(s) 153, 165, 357, 903, 951, 995
Tru9I TTAA 6 cut(s) 153, 165, 357, 903, 951, 995
TscAI CASTG 3 cut(s) 445, 475, 1075
TseFI GTSAC 1 cut(s) 1429
TseI GCWGC 2 cut(s) 896, 1415
Tsp45I GTSAC 1 cut(s) 1429
TspDTI ATGAA 6 cut(s) 159, 249, 310, 330, 780, 1488
TspGWI ACGGA 1 cut(s) 827
TspRI CASTG 3 cut(s) 445, 475, 1075
Tth111I GACNNNGTC 1 cut(s) 452
Van91I CCANNNNNTGG 1 cut(s) 977
Vha464I CTTAAG 1 cut(s) 152
XapI RAATTY 5 cut(s) 317, 587, 829, 879, 1255
XceI RCATGY 2 cut(s) 674, 1346
XmiI GTMKAC 1 cut(s) 675
XmnI GAANNNNTTC 3 cut(s) 48, 131, 631
XspI CTAG 5 cut(s) 20, 101, 426, 1161, 1398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.