Rh2CG514600

Belongs to the argonaute family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002050
Physical Location & Seq
Reverse (-)
68858262 .. 68860734
2473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG514600.1

Sequence Viewer

Length: 855 bp
ATGATGGTAGCTGGTGCCGAAAATACAGGTGGGGACGCAGATTTGCCCCCACCCCCTCCAACCATGCCATCCAATTTGAAGCCAGGACTAGTGGCTCCGCCCAAGTATTCTATTATGACTAGGCCATGCAGGACTGGAACTACTGGACGGCGCATAACTTTGCTTGCAAACCACTTCAAAGTTTCAGTGAATAATCCAGATGCTGTATTTTACCAGTATTCTGTTCACATGACTTATGAAGATAATAAGCCAGTTCTTCCGGGCATTGGAATTGGGAGAAAATTGATGGATAGGCTTTATCAAACATACTCCGTGGAATTTGATGGCAAAAAGTTTGCATATGATGGGGAGAAAGCTCTGTACACAGTTGGCCCTCTTCCGCTGCACAAGCTCAATTACACAGTTGTGCTAGAGGAATCAGTTTCTAGGCCACATGAAACCGGGAGCTCTAGTGTCACTGAAAAACGGATGAAACAATCTATTCAGTCAAAGACTTTCAATGTAGAGATAAGTTATGCTGCCAAAATACCTCTGAAGCCAATTGTTCTTGCCCTTGAAGGAGCTGATGCAGATGATGGTCAAGATGCATTGAGAGTGCTCAACATAGTCTTAAGGCAGCAAGCAGCTAACAGGGTGTACAAAACCCCCCTCAACATTGACTGGGAAAGGGGTGTAAGGAGTTATGGCTATAATGATGATCCTGTGCTTGCTGCATGCGGTATATCCATAGACAAGCAATTGACTCAGATTGAAGGTCATGTACTTGAGGCTCCGAAGTTAAAGGTCGGTAACAATGAGGACATGATGCCCTTTAAGGGACGATGGAACTTCAAAGGAAAGATCCAGAAGCTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0002252 GO:0002376 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006325 GO:0006342 GO:0006355 GO:0006396 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0006996 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009615 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010467 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0016043 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019219 GO:0019222 GO:0030422 GO:0031047 GO:0031050 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0032259 GO:0034641 GO:0035194 GO:0035197 GO:0040029 GO:0042221 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0045814 GO:0045892 GO:0045934 GO:0046483 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051276 GO:0051607 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0061980 GO:0065007 GO:0070887 GO:0070918 GO:0071310 GO:0071359 GO:0071407 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0097159 GO:0098542 GO:1901360 GO:1901363 GO:1901698 GO:1901699 GO:1902679 GO:1903506 GO:1903507 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArgoL1 ArgoL2 ArgoMid ArgoN PAZ Piwi

Protein Analysis

284

Amino Acids

31.52

Weight (kDa)

9.1

Isoelectric Point (pI)

39.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArgoN PF16486 50 - 204 3.8e-27 N-terminal domain of argonaute
ArgoL2 PF16488 223 - 261 6.5e-11 Argonaute linker 2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 14
AciI CCGC 3 cut(s) 98, 380, 717
AclWI GGATC 2 cut(s) 692, 835
AcsI RAATTY 1 cut(s) 317
AcuI CTGAAG 1 cut(s) 554
AfaI GTAC 3 cut(s) 362, 638, 762
AfiI CCNNNNNNNGG 2 cut(s) 266, 815
AflII CTTAAG 1 cut(s) 610
AgsI TTSAA 6 cut(s) 79, 178, 499, 557, 752, 832
AhlI ACTAGT 1 cut(s) 88
AjnI CCWGG 1 cut(s) 82
AleI CACNNNNGTG 1 cut(s) 404
AluBI AGCT 7 cut(s) 11, 356, 391, 447, 563, 626, 850
AluI AGCT 7 cut(s) 11, 356, 391, 447, 563, 626, 850
Alw21I GWGCWC 2 cut(s) 449, 600
AlwI GGATC 2 cut(s) 692, 835
AlwNI CAGNNNCTG 2 cut(s) 203, 850
AoxI GGCC 3 cut(s) 122, 370, 428
ApeKI GCWGC 5 cut(s) 382, 518, 616, 623, 710
ApoI RAATTY 1 cut(s) 317
AspLEI GCGC 1 cut(s) 153
AspS9I GGNCC 1 cut(s) 371
AsuC2I CCSGG 2 cut(s) 261, 442
BanI GGYRCC 1 cut(s) 14
BanII GRGCYC 1 cut(s) 449
BarI GAAGNNNNNNTAC 2 cut(s) 744, 776
Bbv12I GWGCWC 2 cut(s) 449, 600
BbvI GCAGC 5 cut(s) 369, 505, 628, 635, 697
BccI CCATC 6 cut(s) 76, 280, 317, 338, 569, 816
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 1 cut(s) 84
BcnI CCSGG 2 cut(s) 261, 442
BcuI ACTAGT 1 cut(s) 88
BfaI CTAG 5 cut(s) 89, 120, 410, 426, 450
BfrI CTTAAG 1 cut(s) 610
BisI GCNGC 5 cut(s) 383, 519, 617, 624, 711
BlsI GCNGC 5 cut(s) 384, 520, 618, 625, 712
Bme1390I CCNGG 3 cut(s) 84, 261, 442
BmgT120I GGNCC 1 cut(s) 371
BmiI GGNNCC 3 cut(s) 16, 96, 771
BmrFI CCNGG 3 cut(s) 84, 261, 442
BmrI ACTGGG 1 cut(s) 670
BmsI GCATC 4 cut(s) 190, 556, 574, 795
BmuI ACTGGG 1 cut(s) 670
BpuEI CTTGAG 1 cut(s) 785
BpuMI CCSGG 2 cut(s) 261, 442
BsaJI CCNNGG 1 cut(s) 312
BsaXI ACNNNNNCTCC 2 cut(s) 436, 466
Bsc4I CCNNNNNNNGG 2 cut(s) 266, 815
Bse1I ACTGG 5 cut(s) 139, 148, 214, 251, 665
BseBI CCWGG 1 cut(s) 84
BseDI CCNNGG 1 cut(s) 312
BseGI GGATG 2 cut(s) 68, 474
BseLI CCNNNNNNNGG 2 cut(s) 266, 815
BseMII CTCAG 1 cut(s) 758
BseNI ACTGG 5 cut(s) 139, 148, 214, 251, 665
BseXI GCAGC 5 cut(s) 369, 505, 628, 635, 697
BsgI GTGCAG 1 cut(s) 368
BshFI GGCC 3 cut(s) 124, 372, 430
BshNI GGYRCC 1 cut(s) 14
BsiHKAI GWGCWC 2 cut(s) 449, 600
BsiSI CCGG 2 cut(s) 260, 441
BslFI GGGAC 2 cut(s) 47, 831
BslI CCNNNNNNNGG 2 cut(s) 266, 815
BsmFI GGGAC 2 cut(s) 47, 831
BsnI GGCC 3 cut(s) 124, 372, 430
Bsp1286I GDGCHC 2 cut(s) 449, 600
Bsp1407I TGTACA 2 cut(s) 360, 636
Bsp143I GATC 2 cut(s) 697, 840
BspACI CCGC 3 cut(s) 98, 380, 717
BspANI GGCC 3 cut(s) 124, 372, 430
BspCNI CTCAG 1 cut(s) 757
BspLI GGNNCC 3 cut(s) 16, 96, 771
BspPI GGATC 2 cut(s) 692, 835
BspT107I GGYRCC 1 cut(s) 14
BspTI CTTAAG 1 cut(s) 610
BsrGI TGTACA 2 cut(s) 360, 636
BsrI ACTGG 5 cut(s) 139, 148, 214, 251, 665
BssECI CCNNGG 1 cut(s) 312
BssMI GATC 2 cut(s) 697, 840
Bst2UI CCWGG 1 cut(s) 84
Bst4CI ACNGT 2 cut(s) 367, 403
Bst6I CTCTTC 1 cut(s) 381
BstAFI CTTAAG 1 cut(s) 610
BstAUI TGTACA 2 cut(s) 360, 636
BstC8I GCNNGC 4 cut(s) 165, 621, 708, 715
BstDEI CTNAG 1 cut(s) 744
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 2 cut(s) 68, 474
BstHHI GCGC 1 cut(s) 153
BstKTI GATC 2 cut(s) 700, 843
BstMBI GATC 2 cut(s) 697, 840
BstMWI GCNNNNNNNGC 1 cut(s) 388
BstNI CCWGG 1 cut(s) 84
BstNSI RCATGY 1 cut(s) 717
BstSCI CCNGG 3 cut(s) 82, 259, 440
BstV1I GCAGC 5 cut(s) 369, 505, 628, 635, 697
BstX2I RGATCY 1 cut(s) 840
BstYI RGATCY 1 cut(s) 840
BsuRI GGCC 3 cut(s) 124, 372, 430
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 2 cut(s) 68, 474
BtsIMutI CAGTG 2 cut(s) 192, 456
Cac8I GCNNGC 4 cut(s) 165, 621, 708, 715
CaiI CAGNNNCTG 2 cut(s) 203, 850
CfoI GCGC 1 cut(s) 153
Cfr13I GGNCC 1 cut(s) 371
CseI GACGC 1 cut(s) 44
Csp6I GTAC 3 cut(s) 361, 637, 761
CviAII CATG 7 cut(s) 64, 126, 229, 434, 714, 758, 802
CviQI GTAC 3 cut(s) 361, 637, 761
DdeI CTNAG 1 cut(s) 744
DpnI GATC 2 cut(s) 699, 842
DpnII GATC 2 cut(s) 697, 840
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
EciI GGCGGA 1 cut(s) 87
Ecl136II GAGCTC 1 cut(s) 447
Eco24I GRGCYC 1 cut(s) 449
Eco53kI GAGCTC 1 cut(s) 447
Eco57I CTGAAG 1 cut(s) 554
EcoICRI GAGCTC 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 82
EcoT22I ATGCAT 1 cut(s) 589
EcoT38I GRGCYC 1 cut(s) 449
FaeI CATG 7 cut(s) 67, 129, 232, 437, 717, 761, 805
FaqI GGGAC 2 cut(s) 47, 831
FatI CATG 7 cut(s) 63, 125, 228, 433, 713, 757, 801
FauNDI CATATG 1 cut(s) 340
Fnu4HI GCNGC 5 cut(s) 383, 519, 617, 624, 711
FokI GGATG 2 cut(s) 55, 481
FriOI GRGCYC 1 cut(s) 449
Fsp4HI GCNGC 5 cut(s) 383, 519, 617, 624, 711
FspBI CTAG 5 cut(s) 89, 120, 410, 426, 450
GlaI GCGC 1 cut(s) 152
GluI GCNGC 5 cut(s) 383, 519, 617, 624, 711
HaeIII GGCC 3 cut(s) 124, 372, 430
HapII CCGG 2 cut(s) 260, 441
HgaI GACGC 1 cut(s) 44
HhaI GCGC 1 cut(s) 153
Hin1II CATG 7 cut(s) 67, 129, 232, 437, 717, 761, 805
Hin6I GCGC 1 cut(s) 151
HinP1I GCGC 1 cut(s) 151
HinfI GANTC 2 cut(s) 416, 742
HpaII CCGG 2 cut(s) 260, 441
Hpy166II GTNNAC 3 cut(s) 226, 363, 637
Hpy188I TCNGA 3 cut(s) 534, 747, 774
Hpy188III TCNNGA 3 cut(s) 197, 581, 844
Hpy8I GTNNAC 3 cut(s) 226, 363, 637
HpyAV CCTTC 2 cut(s) 551, 746
HpyCH4III ACNGT 2 cut(s) 367, 403
HpyCH4V TGCA 7 cut(s) 129, 167, 338, 385, 569, 587, 713
HpyF10VI GCNNNNNNNGC 1 cut(s) 388
HpyF3I CTNAG 1 cut(s) 744
Hsp92II CATG 7 cut(s) 67, 129, 232, 437, 717, 761, 805
HspAI GCGC 1 cut(s) 151
Kzo9I GATC 2 cut(s) 697, 840
LmnI GCTCC 4 cut(s) 100, 444, 560, 775
Lsp1109I GCAGC 5 cut(s) 369, 505, 628, 635, 697
LweI GCATC 4 cut(s) 190, 556, 574, 795
MaeI CTAG 5 cut(s) 89, 120, 410, 426, 450
MaeIII GTNAC 2 cut(s) 454, 788
MalI GATC 2 cut(s) 699, 842
MboI GATC 2 cut(s) 697, 840
MboII GAAGA 3 cut(s) 248, 251, 368
MfeI CAATTG 2 cut(s) 540, 737
MflI RGATCY 1 cut(s) 840
MhlI GDGCHC 2 cut(s) 449, 600
MluCI AATT 7 cut(s) 73, 270, 281, 317, 394, 540, 737
MlyI GAGTC 1 cut(s) 736
MmeI TCCRAC 1 cut(s) 83
MnlI CCTC 7 cut(s) 66, 384, 406, 540, 659, 760, 790
Mph1103I ATGCAT 1 cut(s) 589
MseI TTAA 3 cut(s) 611, 779, 813
MslI CAYNNNNRTG 1 cut(s) 404
MspA1I CMGCKG 1 cut(s) 382
MspCI CTTAAG 1 cut(s) 610
MspI CCGG 2 cut(s) 260, 441
MspR9I CCNGG 3 cut(s) 84, 261, 442
MunI CAATTG 2 cut(s) 540, 737
MvaI CCWGG 1 cut(s) 84
MwoI GCNNNNNNNGC 1 cut(s) 388
NciI CCSGG 2 cut(s) 261, 442
NdeI CATATG 1 cut(s) 340
NdeII GATC 2 cut(s) 697, 840
NlaIII CATG 7 cut(s) 67, 129, 232, 437, 717, 761, 805
NlaIV GGNNCC 3 cut(s) 16, 96, 771
NmuCI GTSAC 1 cut(s) 454
NsiI ATGCAT 1 cut(s) 589
NspI RCATGY 1 cut(s) 717
OliI CACNNNNGTG 1 cut(s) 404
PaeI GCATGC 1 cut(s) 717
PfeI GAWTC 1 cut(s) 416
PkrI GCNGC 5 cut(s) 384, 520, 618, 625, 712
PleI GAGTC 1 cut(s) 736
PpsI GAGTC 1 cut(s) 736
Psp124BI GAGCTC 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 82
PspGI CCWGG 1 cut(s) 82
PspN4I GGNNCC 3 cut(s) 16, 96, 771
PspPI GGNCC 1 cut(s) 371
PstNI CAGNNNCTG 2 cut(s) 203, 850
PsuI RGATCY 1 cut(s) 840
RsaI GTAC 3 cut(s) 362, 638, 762
RsaNI GTAC 3 cut(s) 361, 637, 761
RseI CAYNNNNRTG 1 cut(s) 404
SacI GAGCTC 1 cut(s) 449
SaqAI TTAA 3 cut(s) 611, 779, 813
SatI GCNGC 5 cut(s) 383, 519, 617, 624, 711
Sau3AI GATC 2 cut(s) 697, 840
Sau96I GGNCC 1 cut(s) 371
SchI GAGTC 1 cut(s) 736
ScrFI CCNGG 3 cut(s) 84, 261, 442
SduI GDGCHC 2 cut(s) 449, 600
SfaNI GCATC 4 cut(s) 190, 556, 574, 795
SmiMI CAYNNNNRTG 1 cut(s) 404
SmlI CTYRAG 2 cut(s) 610, 764
SmoI CTYRAG 2 cut(s) 610, 764
SpeI ACTAGT 1 cut(s) 88
SphI GCATGC 1 cut(s) 717
Sse9I AATT 7 cut(s) 73, 270, 281, 317, 394, 540, 737
SsiI CCGC 3 cut(s) 98, 380, 717
SspMI CTAG 5 cut(s) 89, 120, 410, 426, 450
SstI GAGCTC 1 cut(s) 449
StyD4I CCNGG 3 cut(s) 82, 259, 440
TaaI ACNGT 2 cut(s) 367, 403
TasI AATT 7 cut(s) 73, 270, 281, 317, 394, 540, 737
TatI WGTACW 3 cut(s) 360, 636, 760
TfiI GAWTC 1 cut(s) 416
Tru1I TTAA 3 cut(s) 611, 779, 813
Tru9I TTAA 3 cut(s) 611, 779, 813
TscAI CASTG 2 cut(s) 192, 463
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 5 cut(s) 382, 518, 616, 623, 710
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 3 cut(s) 252, 450, 485
TspGWI ACGGA 2 cut(s) 301, 481
TspRI CASTG 2 cut(s) 192, 463
Vha464I CTTAAG 1 cut(s) 610
XapI RAATTY 1 cut(s) 317
XceI RCATGY 1 cut(s) 717
XspI CTAG 5 cut(s) 89, 120, 410, 426, 450
Zsp2I ATGCAT 1 cut(s) 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.