RLG00000006715

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000642
Physical Location & Seq
Reverse (-)
9885042 .. 9887782
2741 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000006715

Sequence Viewer

Length: 1446 bp
ATGGAGGAGAGTTTGTTGTTGCCAAGAGGCAAAGATCAAGCAGAGCAAAGAAAAGAGTCATGGTGTTTGTTTTTCGAAGAAGTCAAGAGGTTAGGATGCTTAGCAGGACCTATGGTTGCTGTCTTGCTATCCCAGTACATGGTGCAGGTTGTTTCGATGATGATGGTTGGTCACCTTGGTGAGCTTGCTCTCTCCAGCACTGCCTTGGCCATATCCTTTTCCGGGGTCACTGGCTTCAGTGTTCTTCTAGGAATGGCCAGTGCACTTGAAACTTTATGTGGGCAAGCGTATGGAGCTGAGCAATATCAGAAACTTGGACTTCAAACTTACACCGCCATATTTTCTCTCACCTTAGTTTGTCTTCCTTTGTCTTTGATATGGATATATATGGCGGATATACTGATGTTCATGGGCCAAGACCCTGTAATTTCACATGAAGCTGGCAAGTTCACGATCTGGCTTCTTCCTGCTTTGTTTTCTTATGCAACTCTCCAACCACTCAATAGATACTTTCAGACTCAAAGTCTAATAACTCCTATGCTCGTGAGCTCTTTGGCAACTATTTTGTTCCATATTCCTCTCTGTTGGGCTCTAGTATTCAAGTCTGGACTGGATAGCCTTGGAGGGGCATTAGCAATGAGTATTTCCTACTGGTTCAATGCGATTGTACTTGGATTGTTCATGAAGTTTTCTTCTGCCTGTTCGAAAACCAGAGTTTCAATTTCTATGGAGCTATTCCATGGAATGAGAGAGTTCTTTCGTTTTGCCATCCCTTCTGCTGTAATGATATGCCTTGAGTGGTGGTCATTTGAGCTGCTTATCTTGCTGTCTGGGCTTTTACCAAACCCAGCTCTTGAAACTTCAGTTCTGTCTGTATGTCTCCAGACCATTGCGACACTCTATTCAATACCATACGGGGTTGGTGCTGCAGCAAGCACTAGAGTTTCAAATGAACTAGGTGCTGGCAACCCAAAAGGTGCTCGTATGGCTACGTGTGCTGCGATGTTTCTTGCACTCACAGAGACAACTATAATAACCACAACCCTCTTTGCCTGCCGCAATCTGTTTGGCTACACTTTTAGTAATGAGAGAGATGTCGTCGATTACGTCACAACCATGGCTCCTCTAGTTTGTCTCTCTGTTATACTAGACAGTTTTCAAGGGGTCTTTTCAGGTGTTGCTAGAGGATGTGGGTGGCAGCATATAGGGGCTTACGTAAACCTTGGAGCATTTTATTTATGTGGGATTCCTCTTGCTGCTACATTAGCTTTCTGGGTTCAACTAAGAGGAAGGGGTCTTTGGATTGGAATACAAGTTGGAGCTTTTGTGCAAACCACTCTGCTTGCTATTGTAACAACTTGTACGAACTGGGAAAAGCAGGCAAGTAAGGCAAGGGAGAGGATATTTGAGGCAAGCTATGTAGAAGCTAATGGATTGTTGCACTAA

Protein Analysis

482

Amino Acids

52.62

Weight (kDa)

6.32

Isoelectric Point (pI)

29.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 37 - 196 2.1e-31 MatE
MatE PF01554 258 - 420 8.8e-28 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 136
AccB7I CCANNNNNTGG 1 cut(s) 139
AciI CCGC 3 cut(s) 333, 392, 1057
AcoI YGGCCR 2 cut(s) 207, 255
AcuI CTGAAG 2 cut(s) 220, 846
AfaI GTAC 3 cut(s) 137, 669, 1363
AfiI CCNNNNNNNGG 3 cut(s) 139, 222, 625
AflIII ACRYGT 1 cut(s) 992
AhdI GACNNNNNGTC 1 cut(s) 522
AjuI GAANNNNNNNTTGG 2 cut(s) 1282, 1314
AleI CACNNNNGTG 1 cut(s) 177
Alw21I GWGCWC 3 cut(s) 265, 551, 982
Alw26I GTCTC 3 cut(s) 884, 1016, 1139
Alw44I GTGCAC 1 cut(s) 261
AoxI GGCC 3 cut(s) 207, 255, 412
ApaLI GTGCAC 1 cut(s) 261
ApeKI GCWGC 6 cut(s) 814, 926, 929, 998, 1198, 1256
AspS9I GGNCC 2 cut(s) 107, 412
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 3 cut(s) 164, 191, 340
AsuII TTCGAA 2 cut(s) 75, 704
AvaII GGWCC 1 cut(s) 107
BaeGI GKGCMC 1 cut(s) 265
BalI TGGCCA 2 cut(s) 209, 257
BanII GRGCYC 2 cut(s) 551, 592
BauI CACGAG 1 cut(s) 542
BbsI GAAGAC 1 cut(s) 353
Bbv12I GWGCWC 3 cut(s) 265, 551, 982
BbvI GCAGC 6 cut(s) 801, 913, 941, 985, 1210, 1243
BccI CCATC 2 cut(s) 157, 776
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 3 cut(s) 884, 1016, 1139
BfaI CTAG 7 cut(s) 248, 593, 939, 956, 1127, 1148, 1182
BfmI CTRYAG 1 cut(s) 927
BfuAI ACCTGC 1 cut(s) 136
BisI GCNGC 7 cut(s) 815, 927, 930, 999, 1057, 1199, 1257
BlpI GCTNAGC 2 cut(s) 100, 297
BlsI GCNGC 7 cut(s) 816, 928, 931, 1000, 1058, 1200, 1258
Bme1390I CCNGG 1 cut(s) 223
Bme18I GGWCC 1 cut(s) 107
BmeRI GACNNNNNGTC 1 cut(s) 522
BmgT120I GGNCC 2 cut(s) 107, 412
BmiI GGNNCC 1 cut(s) 1122
BmrFI CCNGG 1 cut(s) 223
BmrI ACTGGG 2 cut(s) 127, 1378
BmsI GCATC 1 cut(s) 86
BmuI ACTGGG 2 cut(s) 127, 1378
BpiI GAAGAC 1 cut(s) 353
BpmI CTGGAG 2 cut(s) 178, 866
Bpu1102I GCTNAGC 2 cut(s) 100, 297
Bpu14I TTCGAA 2 cut(s) 75, 704
BpuEI CTTGAG 1 cut(s) 815
BpuMI CCSGG 1 cut(s) 223
BsaAI YACGTR 2 cut(s) 993, 1216
BsaJI CCNNGG 7 cut(s) 175, 204, 222, 619, 739, 1116, 1222
BsaXI ACNNNNNCTCC 5 cut(s) 29, 1105, 1135, 1311, 1341
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 222, 625
Bse1I ACTGG 6 cut(s) 133, 235, 258, 615, 656, 1373
Bse3DI GCAATG 2 cut(s) 642, 888
BseDI CCNNGG 7 cut(s) 175, 204, 222, 619, 739, 1116, 1222
BseGI GGATG 3 cut(s) 101, 768, 1193
BseLI CCNNNNNNNGG 3 cut(s) 139, 222, 625
BseMI GCAATG 2 cut(s) 642, 888
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 6 cut(s) 133, 235, 258, 615, 656, 1373
BseRI GAGGAG 2 cut(s) 20, 1113
BseSI GKGCMC 1 cut(s) 265
BseXI GCAGC 6 cut(s) 801, 913, 941, 985, 1210, 1243
BseYI CCCAGC 1 cut(s) 847
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 3 cut(s) 209, 257, 414
BsiHKAI GWGCWC 3 cut(s) 265, 551, 982
BsiSI CCGG 1 cut(s) 222
BslI CCNNNNNNNGG 3 cut(s) 139, 222, 625
BsmAI GTCTC 3 cut(s) 884, 1016, 1139
BsnI GGCC 3 cut(s) 209, 257, 414
Bsp119I TTCGAA 2 cut(s) 75, 704
Bsp1286I GDGCHC 4 cut(s) 265, 551, 592, 982
Bsp143I GATC 2 cut(s) 34, 453
Bsp1720I GCTNAGC 2 cut(s) 100, 297
Bsp19I CCATGG 2 cut(s) 739, 1116
BspACI CCGC 3 cut(s) 333, 392, 1057
BspANI GGCC 3 cut(s) 209, 257, 414
BspCNI CTCAG 1 cut(s) 289
BspHI TCATGA 1 cut(s) 681
BspLI GGNNCC 1 cut(s) 1122
BspMAI CTGCAG 1 cut(s) 931
BspMI ACCTGC 1 cut(s) 136
BspT104I TTCGAA 2 cut(s) 75, 704
BsrDI GCAATG 2 cut(s) 642, 888
BsrI ACTGG 6 cut(s) 133, 235, 258, 615, 656, 1373
BssECI CCNNGG 7 cut(s) 175, 204, 222, 619, 739, 1116, 1222
BssMI GATC 2 cut(s) 34, 453
BssSI CACGAG 1 cut(s) 542
BssT1I CCWWGG 6 cut(s) 175, 204, 619, 739, 1116, 1222
Bst2BI CACGAG 1 cut(s) 542
Bst4CI ACNGT 1 cut(s) 1154
BstBAI YACGTR 2 cut(s) 993, 1216
BstBI TTCGAA 2 cut(s) 75, 704
BstC8I GCNNGC 9 cut(s) 186, 285, 442, 934, 964, 1054, 1344, 1380, 1414
BstDEI CTNAG 4 cut(s) 100, 297, 352, 1283
BstDSI CCRYGG 2 cut(s) 739, 1116
BstEII GGTNACC 1 cut(s) 170
BstF5I GGATG 3 cut(s) 101, 768, 1193
BstKTI GATC 2 cut(s) 37, 456
BstMAI GTCTC 3 cut(s) 884, 1016, 1139
BstMBI GATC 2 cut(s) 34, 453
BstMWI GCNNNNNNNGC 7 cut(s) 293, 823, 832, 986, 995, 1265, 1388
BstPI GGTNACC 1 cut(s) 170
BstSCI CCNGG 1 cut(s) 221
BstSFI CTRYAG 1 cut(s) 927
BstSLI GKGCMC 1 cut(s) 265
BstSNI TACGTA 1 cut(s) 1216
BstV1I GCAGC 6 cut(s) 801, 913, 941, 985, 1210, 1243
BstV2I GAAGAC 1 cut(s) 353
BsuRI GGCC 3 cut(s) 209, 257, 414
BtgI CCRYGG 2 cut(s) 739, 1116
BtgZI GCGATG 1 cut(s) 1016
BtsCI GGATG 3 cut(s) 101, 768, 1193
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 4 cut(s) 198, 228, 244, 265
BveI ACCTGC 1 cut(s) 136
Cac8I GCNNGC 9 cut(s) 186, 285, 442, 934, 964, 1054, 1344, 1380, 1414
CciI TCATGA 1 cut(s) 681
Cfr13I GGNCC 2 cut(s) 107, 412
Csp6I GTAC 3 cut(s) 136, 668, 1362
CspCI CAANNNNNGTGG 2 cut(s) 1324, 1359
CviAII CATG 7 cut(s) 60, 139, 409, 434, 682, 740, 1117
CviQI GTAC 3 cut(s) 136, 668, 1362
DdeI CTNAG 4 cut(s) 100, 297, 352, 1283
DpnI GATC 2 cut(s) 36, 455
DpnII GATC 2 cut(s) 34, 453
DriI GACNNNNNGTC 1 cut(s) 522
EaeI YGGCCR 2 cut(s) 207, 255
Eam1105I GACNNNNNGTC 1 cut(s) 522
EciI GGCGGA 1 cut(s) 407
Ecl136II GAGCTC 1 cut(s) 549
Eco105I TACGTA 1 cut(s) 1216
Eco130I CCWWGG 6 cut(s) 175, 204, 619, 739, 1116, 1222
Eco24I GRGCYC 2 cut(s) 551, 592
Eco47I GGWCC 1 cut(s) 107
Eco53kI GAGCTC 1 cut(s) 549
Eco57I CTGAAG 2 cut(s) 220, 846
Eco91I GGTNACC 1 cut(s) 170
EcoICRI GAGCTC 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 107
EcoO65I GGTNACC 1 cut(s) 170
EcoT14I CCWWGG 6 cut(s) 175, 204, 619, 739, 1116, 1222
EcoT38I GRGCYC 2 cut(s) 551, 592
ErhI CCWWGG 6 cut(s) 175, 204, 619, 739, 1116, 1222
FaeI CATG 7 cut(s) 63, 142, 412, 437, 685, 743, 1120
FatI CATG 7 cut(s) 59, 138, 408, 433, 681, 739, 1116
Fnu4HI GCNGC 7 cut(s) 815, 927, 930, 999, 1057, 1199, 1257
FokI GGATG 3 cut(s) 108, 755, 1200
FriOI GRGCYC 2 cut(s) 551, 592
Fsp4HI GCNGC 7 cut(s) 815, 927, 930, 999, 1057, 1199, 1257
FspBI CTAG 7 cut(s) 248, 593, 939, 956, 1127, 1148, 1182
GluI GCNGC 7 cut(s) 815, 927, 930, 999, 1057, 1199, 1257
GsaI CCCAGC 1 cut(s) 851
GsuI CTGGAG 2 cut(s) 178, 866
HaeIII GGCC 3 cut(s) 209, 257, 414
HapII CCGG 1 cut(s) 222
Hin1II CATG 7 cut(s) 63, 142, 412, 437, 685, 743, 1120
HinfI GANTC 3 cut(s) 56, 517, 1246
HpaII CCGG 1 cut(s) 222
HphI GGTGA 3 cut(s) 164, 191, 340
Hpy166II GTNNAC 3 cut(s) 263, 450, 1219
Hpy188I TCNGA 2 cut(s) 309, 516
Hpy188III TCNNGA 7 cut(s) 85, 451, 544, 606, 682, 854, 883
Hpy8I GTNNAC 3 cut(s) 263, 450, 1219
Hpy99I CGWCG 1 cut(s) 1103
HpyAV CCTTC 2 cut(s) 783, 1284
HpyCH4III ACNGT 1 cut(s) 1154
HpyCH4IV ACGT 3 cut(s) 992, 1107, 1215
HpyCH4V TGCA 7 cut(s) 145, 263, 485, 929, 1013, 1330, 1441
HpyF10VI GCNNNNNNNGC 7 cut(s) 293, 823, 832, 986, 995, 1265, 1388
HpyF3I CTNAG 4 cut(s) 100, 297, 352, 1283
HpySE526I ACGT 3 cut(s) 992, 1107, 1215
Hsp92II CATG 7 cut(s) 63, 142, 412, 437, 685, 743, 1120
Kzo9I GATC 2 cut(s) 34, 453
LmnI GCTCC 5 cut(s) 293, 730, 1126, 1226, 1319
Lsp1109I GCAGC 6 cut(s) 801, 913, 941, 985, 1210, 1243
LweI GCATC 1 cut(s) 86
MaeI CTAG 7 cut(s) 248, 593, 939, 956, 1127, 1148, 1182
MaeII ACGT 3 cut(s) 992, 1107, 1215
MaeIII GTNAC 4 cut(s) 170, 226, 1108, 1351
MalI GATC 2 cut(s) 36, 455
MboI GATC 2 cut(s) 34, 453
MboII GAAGA 5 cut(s) 89, 236, 353, 455, 684
MhlI GDGCHC 4 cut(s) 265, 551, 592, 982
MlsI TGGCCA 2 cut(s) 209, 257
MluCI AATT 2 cut(s) 426, 720
MluNI TGGCCA 2 cut(s) 209, 257
MlyI GAGTC 2 cut(s) 65, 511
MmeI TCCRAC 2 cut(s) 517, 1297
Mox20I TGGCCA 2 cut(s) 209, 257
MscI TGGCCA 2 cut(s) 209, 257
MslI CAYNNNNRTG 2 cut(s) 177, 1115
Msp20I TGGCCA 2 cut(s) 209, 257
MspI CCGG 1 cut(s) 222
MspR9I CCNGG 1 cut(s) 223
MwoI GCNNNNNNNGC 7 cut(s) 293, 823, 832, 986, 995, 1265, 1388
NciI CCSGG 1 cut(s) 223
NcoI CCATGG 2 cut(s) 739, 1116
NdeII GATC 2 cut(s) 34, 453
NlaIII CATG 7 cut(s) 63, 142, 412, 437, 685, 743, 1120
NlaIV GGNNCC 1 cut(s) 1122
NmuCI GTSAC 3 cut(s) 170, 226, 1108
NspV TTCGAA 2 cut(s) 75, 704
OliI CACNNNNGTG 1 cut(s) 177
PagI TCATGA 1 cut(s) 681
PcsI WCGNNNNNNNCGW 2 cut(s) 998, 1104
PfeI GAWTC 1 cut(s) 1246
PflMI CCANNNNNTGG 1 cut(s) 139
PkrI GCNGC 7 cut(s) 816, 928, 931, 1000, 1058, 1200, 1258
PleI GAGTC 2 cut(s) 64, 511
PpsI GAGTC 2 cut(s) 64, 511
Ppu21I YACGTR 2 cut(s) 993, 1216
PpuMI RGGWCCY 1 cut(s) 107
Psp124BI GAGCTC 1 cut(s) 551
Psp5II RGGWCCY 1 cut(s) 107
PspEI GGTNACC 1 cut(s) 170
PspFI CCCAGC 1 cut(s) 847
PspN4I GGNNCC 1 cut(s) 1122
PspPI GGNCC 2 cut(s) 107, 412
PspPPI RGGWCCY 1 cut(s) 107
PstI CTGCAG 1 cut(s) 931
RsaI GTAC 3 cut(s) 137, 669, 1363
RsaNI GTAC 3 cut(s) 136, 668, 1362
RseI CAYNNNNRTG 2 cut(s) 177, 1115
SacI GAGCTC 1 cut(s) 551
SatI GCNGC 7 cut(s) 815, 927, 930, 999, 1057, 1199, 1257
Sau3AI GATC 2 cut(s) 34, 453
Sau96I GGNCC 2 cut(s) 107, 412
SchI GAGTC 2 cut(s) 65, 511
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 4 cut(s) 265, 551, 592, 982
SfaNI GCATC 1 cut(s) 86
SfcI CTRYAG 1 cut(s) 927
SfuI TTCGAA 2 cut(s) 75, 704
SinI GGWCC 1 cut(s) 107
SmiMI CAYNNNNRTG 2 cut(s) 177, 1115
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
SnaBI TACGTA 1 cut(s) 1216
Sse9I AATT 2 cut(s) 426, 720
SsiI CCGC 3 cut(s) 333, 392, 1057
SspMI CTAG 7 cut(s) 248, 593, 939, 956, 1127, 1148, 1182
SstI GAGCTC 1 cut(s) 551
StyD4I CCNGG 1 cut(s) 221
StyI CCWWGG 6 cut(s) 175, 204, 619, 739, 1116, 1222
TaaI ACNGT 1 cut(s) 1154
TaiI ACGT 3 cut(s) 995, 1110, 1218
TaqI TCGA 4 cut(s) 75, 155, 704, 1101
TasI AATT 2 cut(s) 426, 720
TatI WGTACW 2 cut(s) 135, 667
TauI GCSGC 1 cut(s) 1059
TfiI GAWTC 1 cut(s) 1246
TscAI CASTG 4 cut(s) 205, 235, 244, 265
TseFI GTSAC 3 cut(s) 170, 226, 1108
TseI GCWGC 6 cut(s) 814, 926, 929, 998, 1198, 1256
Tsp45I GTSAC 3 cut(s) 170, 226, 1108
TspDTI ATGAA 5 cut(s) 397, 450, 670, 698, 966
TspRI CASTG 4 cut(s) 205, 235, 244, 265
Van91I CCANNNNNTGG 1 cut(s) 139
VneI GTGCAC 1 cut(s) 261
VpaK11BI GGWCC 1 cut(s) 107
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XspI CTAG 7 cut(s) 248, 593, 939, 956, 1127, 1148, 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.