Rh4CG350600

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000642
Physical Location & Seq
Forward (+)
60769787 .. 60781235
11449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG350600.1

Sequence Viewer

Length: 1446 bp
ATGGAGGAGAGTTTGTTATTCCCAAGAGGGAAAGATCAAGCAGAGCAAAGAAAAGAGTCATGGGGTATGTTTTTCGAAGAAGTCAAGAGGTTGGGTTGCTTAGCCGGACCTATGGTTGCTGTCTTGCTATCCCAGTACATGGTGCAGGTTGTTTCGATGATGATGGTTGGTCACCTTGGTGAGCTTACTCTCTCCAGCACTGCCTTGGCCATATCCTTTTCCGGGGTCACCGGCTTTAGTGTTCTTCTAGGAATGGCCAGTGCACTTGAAACTTTATGTGGGCAAGCGTATGGAGCTGAGCAATATCAGAAACTTGGACTTCAAACTTACACCGCCATATTTTCTCTCACCTTAGTTTGTCTTCCTTTGTCTTTGATATGGATATATATGGCGGATATACTGATGTTCATGGGCCAAGACCCTGTAATTTCACATGAAGCTGGCAAATTCACAATCTGGCTTCTTCCTGCTTTGTTTTCTTATGCAACTCTCCAACCACTCAATAGATACTTTCAGACTCAAAGTCTAATAACTCCTATGCTCGTGAGCTCTTTGGCGACTATTTTGTTCCATATTCCTCTCTGTTGGGCTCTAGTATTCAAGTCTGGACTGGATAGCCTTGGAGGGGCATTAGCAATGAGTATTTCCTACTGGTTCAATGCGATTGTACTTGGATTGTTCATGAAGTTTTCTTCTGCCTGTTCGAAAACCAGAGTTTCAATTTCTATGGAGCTATTCCATGGAATGAGAGAGTTCTTTCGTTTTGCCATCCCTTCTGCTGTAATGATATGCCTTGAGTGGTGGTCATTTGAGATGCTTATCTTGCTGTCCGGAATTCTACAAAATCCGGCTCTTGAAACTTCAGTTCTGTCTGTATGTCTCCAGACCATTGTGACACTCTATTACATACCATATGGGTTTGCTGCTGCAGCAAGTGTTAGAGTTTCCAACGAACTAGGAGCTGGTAACCCACAAGGTGCTCGTATAGCTACATGTGCTGCAATGTTTCTTGCAATCACAGAGACAACTATAGTAACCACAACTCTCTTTGCTTGCCGAAATGTATTTGGTTATGTTTTTAGCAATGAGCAAGAAGTTGTTGATTATGTCACAACCATGGCTCCTCTAGTTTGCTTGTCTGTTTTACTAGACAGCTTGCAAGCCGTCCTTTCAGGTATTGCTAGAGGGTGTGGGTGGCAGCACATAGGGGCTTACATAAACCTTGCAGCATTTTATATTTTTGGAATTCCAATTGCTGCCTTACTGGCTTTCTGGGTACAACTAGGAGGAAGGGGCCTTTGGATTGGAATCCAAGTTGGTGCTTTTGTGCAAACAATTCTGCTTGCTTTTGTCACGACTTGTACAAATTGGGATAAACAGGCACAGAAGGCGAGGGCTAGGATATATGAGGGAAGCTCTCTCGAAGCTAATGGATTGCTGCACTAA

Protein Analysis

481

Amino Acids

52.7

Weight (kDa)

5.9

Isoelectric Point (pI)

31.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 37 - 196 5.1e-31 MatE
MatE PF01554 258 - 420 3.3e-26 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 136
AccB7I CCANNNNNTGG 1 cut(s) 139
AccIII TCCGGA 1 cut(s) 830
AciI CCGC 2 cut(s) 333, 392
AcoI YGGCCR 2 cut(s) 207, 255
AcsI RAATTY 3 cut(s) 446, 834, 1245
AcuI CTGAAG 1 cut(s) 846
AdeI CACNNNGTG 1 cut(s) 977
AfaI GTAC 4 cut(s) 137, 669, 1278, 1363
AfiI CCNNNNNNNGG 3 cut(s) 139, 222, 625
AflIII ACRYGT 1 cut(s) 992
AgsI TTSAA 6 cut(s) 269, 323, 601, 658, 720, 857
AhdI GACNNNNNGTC 1 cut(s) 522
AjuI GAANNNNNNNTTGG 2 cut(s) 1282, 1314
AleI CACNNNNGTG 1 cut(s) 177
Alw21I GWGCWC 3 cut(s) 265, 551, 982
Alw26I GTCTC 2 cut(s) 884, 1016
Alw44I GTGCAC 1 cut(s) 261
Aor13HI TCCGGA 1 cut(s) 830
AoxI GGCC 4 cut(s) 207, 255, 412, 1294
ApaLI GTGCAC 1 cut(s) 261
ApeKI GCWGC 8 cut(s) 923, 926, 929, 998, 1198, 1226, 1256, 1438
ApoI RAATTY 3 cut(s) 446, 834, 1245
AspS9I GGNCC 3 cut(s) 107, 412, 1294
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 4 cut(s) 164, 191, 220, 340
AsuII TTCGAA 2 cut(s) 75, 704
AvaII GGWCC 1 cut(s) 107
BaeGI GKGCMC 1 cut(s) 265
BalI TGGCCA 2 cut(s) 209, 257
BanII GRGCYC 2 cut(s) 551, 592
BauI CACGAG 1 cut(s) 542
BbsI GAAGAC 1 cut(s) 353
Bbv12I GWGCWC 3 cut(s) 265, 551, 982
BbvI GCAGC 8 cut(s) 910, 913, 941, 985, 1210, 1238, 1243, 1425
BccI CCATC 2 cut(s) 157, 776
BceAI ACGGC 1 cut(s) 1148
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 2 cut(s) 884, 1016
BfaI CTAG 8 cut(s) 248, 593, 956, 1127, 1148, 1182, 1283, 1398
BfmI CTRYAG 2 cut(s) 927, 1029
BfuAI ACCTGC 1 cut(s) 136
BglI GCCNNNNNGGC 1 cut(s) 1265
BisI GCNGC 8 cut(s) 924, 927, 930, 999, 1199, 1227, 1257, 1439
BlpI GCTNAGC 2 cut(s) 100, 297
BlsI GCNGC 8 cut(s) 925, 928, 931, 1000, 1200, 1228, 1258, 1440
Bme1390I CCNGG 1 cut(s) 223
Bme18I GGWCC 1 cut(s) 107
BmeRI GACNNNNNGTC 1 cut(s) 522
BmgT120I GGNCC 3 cut(s) 107, 412, 1294
BmiI GGNNCC 2 cut(s) 1122, 1295
BmrFI CCNGG 1 cut(s) 223
BmrI ACTGGG 1 cut(s) 127
BmsI GCATC 1 cut(s) 804
BmuI ACTGGG 1 cut(s) 127
BpiI GAAGAC 1 cut(s) 353
BplI GAGNNNNNCTC 2 cut(s) 1400, 1432
BpmI CTGGAG 2 cut(s) 178, 866
Bpu1102I GCTNAGC 2 cut(s) 100, 297
Bpu14I TTCGAA 2 cut(s) 75, 704
BpuEI CTTGAG 1 cut(s) 815
BpuMI CCSGG 1 cut(s) 223
BsaBI GATNNNNATC 2 cut(s) 818, 1307
BsaJI CCNNGG 6 cut(s) 175, 204, 222, 619, 739, 1116
BsaWI WCCGGW 1 cut(s) 830
BsaXI ACNNNNNCTCC 3 cut(s) 29, 1105, 1135
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 222, 625
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 5 cut(s) 133, 258, 615, 656, 1269
Bse3DI GCAATG 3 cut(s) 642, 1008, 1090
Bse8I GATNNNNATC 2 cut(s) 818, 1307
BseAI TCCGGA 1 cut(s) 830
BseDI CCNNGG 6 cut(s) 175, 204, 222, 619, 739, 1116
BseGI GGATG 1 cut(s) 768
BseJI GATNNNNATC 2 cut(s) 818, 1307
BseLI CCNNNNNNNGG 3 cut(s) 139, 222, 625
BseMI GCAATG 3 cut(s) 642, 1008, 1090
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 5 cut(s) 133, 258, 615, 656, 1269
BseRI GAGGAG 2 cut(s) 20, 1113
BseSI GKGCMC 1 cut(s) 265
BseXI GCAGC 8 cut(s) 910, 913, 941, 985, 1210, 1238, 1243, 1425
BsgI GTGCAG 2 cut(s) 164, 1424
BshFI GGCC 4 cut(s) 209, 257, 414, 1296
BsiHKAI GWGCWC 3 cut(s) 265, 551, 982
BsiSI CCGG 5 cut(s) 105, 222, 231, 831, 848
BslI CCNNNNNNNGG 3 cut(s) 139, 222, 625
BsmAI GTCTC 2 cut(s) 884, 1016
BsnI GGCC 4 cut(s) 209, 257, 414, 1296
Bsp119I TTCGAA 2 cut(s) 75, 704
Bsp1286I GDGCHC 4 cut(s) 265, 551, 592, 982
Bsp13I TCCGGA 1 cut(s) 830
Bsp1407I TGTACA 1 cut(s) 1361
Bsp143I GATC 1 cut(s) 34
Bsp1720I GCTNAGC 2 cut(s) 100, 297
Bsp19I CCATGG 2 cut(s) 739, 1116
BspACI CCGC 2 cut(s) 333, 392
BspANI GGCC 4 cut(s) 209, 257, 414, 1296
BspCNI CTCAG 1 cut(s) 289
BspEI TCCGGA 1 cut(s) 830
BspHI TCATGA 1 cut(s) 681
BspLI GGNNCC 2 cut(s) 1122, 1295
BspMAI CTGCAG 1 cut(s) 931
BspMI ACCTGC 1 cut(s) 136
BspT104I TTCGAA 2 cut(s) 75, 704
BsrDI GCAATG 3 cut(s) 642, 1008, 1090
BsrFI RCCGGY 1 cut(s) 230
BsrGI TGTACA 1 cut(s) 1361
BsrI ACTGG 5 cut(s) 133, 258, 615, 656, 1269
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 6 cut(s) 175, 204, 222, 619, 739, 1116
BssMI GATC 1 cut(s) 34
BssSI CACGAG 1 cut(s) 542
BssT1I CCWWGG 5 cut(s) 175, 204, 619, 739, 1116
Bst2BI CACGAG 1 cut(s) 542
BstAUI TGTACA 1 cut(s) 1361
BstBI TTCGAA 2 cut(s) 75, 704
BstC8I GCNNGC 6 cut(s) 285, 442, 1054, 1157, 1161, 1344
BstDEI CTNAG 3 cut(s) 100, 297, 352
BstDSI CCRYGG 2 cut(s) 739, 1116
BstEII GGTNACC 3 cut(s) 170, 226, 965
BstF5I GGATG 1 cut(s) 768
BstKTI GATC 1 cut(s) 37
BstMAI GTCTC 2 cut(s) 884, 1016
BstMBI GATC 1 cut(s) 34
BstMWI GCNNNNNNNGC 7 cut(s) 293, 823, 929, 986, 995, 1265, 1388
BstNSI RCATGY 1 cut(s) 996
BstPI GGTNACC 3 cut(s) 170, 226, 965
BstSCI CCNGG 1 cut(s) 221
BstSFI CTRYAG 2 cut(s) 927, 1029
BstSLI GKGCMC 1 cut(s) 265
BstV1I GCAGC 8 cut(s) 910, 913, 941, 985, 1210, 1238, 1243, 1425
BstV2I GAAGAC 1 cut(s) 353
BsuRI GGCC 4 cut(s) 209, 257, 414, 1296
BtgI CCRYGG 2 cut(s) 739, 1116
BtsCI GGATG 1 cut(s) 768
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 198, 265
BveI ACCTGC 1 cut(s) 136
Cac8I GCNNGC 6 cut(s) 285, 442, 1054, 1157, 1161, 1344
CciI TCATGA 1 cut(s) 681
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 3 cut(s) 107, 412, 1294
Csp6I GTAC 4 cut(s) 136, 668, 1277, 1362
CviAII CATG 8 cut(s) 60, 139, 409, 434, 682, 740, 993, 1117
CviQI GTAC 4 cut(s) 136, 668, 1277, 1362
DdeI CTNAG 3 cut(s) 100, 297, 352
DpnI GATC 1 cut(s) 36
DpnII GATC 1 cut(s) 34
DraIII CACNNNGTG 1 cut(s) 977
DriI GACNNNNNGTC 1 cut(s) 522
EaeI YGGCCR 2 cut(s) 207, 255
Eam1105I GACNNNNNGTC 1 cut(s) 522
EciI GGCGGA 1 cut(s) 407
Ecl136II GAGCTC 1 cut(s) 549
Eco130I CCWWGG 5 cut(s) 175, 204, 619, 739, 1116
Eco24I GRGCYC 2 cut(s) 551, 592
Eco47I GGWCC 1 cut(s) 107
Eco53kI GAGCTC 1 cut(s) 549
Eco57I CTGAAG 1 cut(s) 846
Eco91I GGTNACC 3 cut(s) 170, 226, 965
EcoICRI GAGCTC 1 cut(s) 549
EcoO109I RGGNCCY 1 cut(s) 1294
EcoO65I GGTNACC 3 cut(s) 170, 226, 965
EcoRI GAATTC 2 cut(s) 834, 1245
EcoT14I CCWWGG 5 cut(s) 175, 204, 619, 739, 1116
EcoT38I GRGCYC 2 cut(s) 551, 592
ErhI CCWWGG 5 cut(s) 175, 204, 619, 739, 1116
FaeI CATG 8 cut(s) 63, 142, 412, 437, 685, 743, 996, 1120
FalI AAGNNNNNCTT 2 cut(s) 1152, 1184
FatI CATG 8 cut(s) 59, 138, 408, 433, 681, 739, 992, 1116
FauNDI CATATG 1 cut(s) 913
Fnu4HI GCNGC 8 cut(s) 924, 927, 930, 999, 1199, 1227, 1257, 1439
FokI GGATG 1 cut(s) 755
FriOI GRGCYC 2 cut(s) 551, 592
Fsp4HI GCNGC 8 cut(s) 924, 927, 930, 999, 1199, 1227, 1257, 1439
FspBI CTAG 8 cut(s) 248, 593, 956, 1127, 1148, 1182, 1283, 1398
GluI GCNGC 8 cut(s) 924, 927, 930, 999, 1199, 1227, 1257, 1439
GsuI CTGGAG 2 cut(s) 178, 866
HaeIII GGCC 4 cut(s) 209, 257, 414, 1296
HapII CCGG 5 cut(s) 105, 222, 231, 831, 848
Hin1II CATG 8 cut(s) 63, 142, 412, 437, 685, 743, 996, 1120
HinfI GANTC 3 cut(s) 56, 517, 1308
HpaII CCGG 5 cut(s) 105, 222, 231, 831, 848
HphI GGTGA 4 cut(s) 164, 191, 220, 340
Hpy166II GTNNAC 1 cut(s) 263
Hpy188I TCNGA 2 cut(s) 309, 516
Hpy188III TCNNGA 9 cut(s) 85, 544, 606, 682, 831, 854, 883, 1354, 1421
Hpy8I GTNNAC 1 cut(s) 263
HpyAV CCTTC 3 cut(s) 783, 1284, 1381
HpyF10VI GCNNNNNNNGC 7 cut(s) 293, 823, 929, 986, 995, 1265, 1388
HpyF3I CTNAG 3 cut(s) 100, 297, 352
Hsp92II CATG 8 cut(s) 63, 142, 412, 437, 685, 743, 996, 1120
Kpn2I TCCGGA 1 cut(s) 830
Kzo9I GATC 1 cut(s) 34
LmnI GCTCC 4 cut(s) 293, 730, 959, 1126
Lsp1109I GCAGC 8 cut(s) 910, 913, 941, 985, 1210, 1238, 1243, 1425
LweI GCATC 1 cut(s) 804
MaeI CTAG 8 cut(s) 248, 593, 956, 1127, 1148, 1182, 1283, 1398
MaeIII GTNAC 7 cut(s) 170, 226, 892, 965, 1033, 1108, 1351
MalI GATC 1 cut(s) 36
MboI GATC 1 cut(s) 34
MboII GAAGA 5 cut(s) 89, 236, 353, 455, 684
MfeI CAATTG 1 cut(s) 1251
MhlI GDGCHC 4 cut(s) 265, 551, 592, 982
MlsI TGGCCA 2 cut(s) 209, 257
MluCI AATT 8 cut(s) 426, 446, 720, 834, 1245, 1251, 1335, 1366
MluNI TGGCCA 2 cut(s) 209, 257
MlyI GAGTC 2 cut(s) 65, 511
MmeI TCCRAC 2 cut(s) 517, 972
MnlI CCTC 9 cut(s) 20, 81, 588, 617, 1134, 1178, 1280, 1386, 1402
Mox20I TGGCCA 2 cut(s) 209, 257
MroI TCCGGA 1 cut(s) 830
MscI TGGCCA 2 cut(s) 209, 257
MslI CAYNNNNRTG 2 cut(s) 177, 1115
Msp20I TGGCCA 2 cut(s) 209, 257
MspI CCGG 5 cut(s) 105, 222, 231, 831, 848
MspR9I CCNGG 1 cut(s) 223
MunI CAATTG 1 cut(s) 1251
MwoI GCNNNNNNNGC 7 cut(s) 293, 823, 929, 986, 995, 1265, 1388
NciI CCSGG 1 cut(s) 223
NcoI CCATGG 2 cut(s) 739, 1116
NdeI CATATG 1 cut(s) 913
NdeII GATC 1 cut(s) 34
NlaIII CATG 8 cut(s) 63, 142, 412, 437, 685, 743, 996, 1120
NlaIV GGNNCC 2 cut(s) 1122, 1295
NmuCI GTSAC 5 cut(s) 170, 226, 892, 1108, 1351
NspI RCATGY 1 cut(s) 996
NspV TTCGAA 2 cut(s) 75, 704
OliI CACNNNNGTG 1 cut(s) 177
PagI TCATGA 1 cut(s) 681
PciI ACATGT 1 cut(s) 992
PfeI GAWTC 1 cut(s) 1308
PflMI CCANNNNNTGG 1 cut(s) 139
PkrI GCNGC 8 cut(s) 925, 928, 931, 1000, 1200, 1228, 1258, 1440
PleI GAGTC 2 cut(s) 64, 511
PpsI GAGTC 2 cut(s) 64, 511
PscI ACATGT 1 cut(s) 992
Psp124BI GAGCTC 1 cut(s) 551
PspEI GGTNACC 3 cut(s) 170, 226, 965
PspN4I GGNNCC 2 cut(s) 1122, 1295
PspPI GGNCC 3 cut(s) 107, 412, 1294
PstI CTGCAG 1 cut(s) 931
RsaI GTAC 4 cut(s) 137, 669, 1278, 1363
RsaNI GTAC 4 cut(s) 136, 668, 1277, 1362
RseI CAYNNNNRTG 2 cut(s) 177, 1115
SacI GAGCTC 1 cut(s) 551
SatI GCNGC 8 cut(s) 924, 927, 930, 999, 1199, 1227, 1257, 1439
Sau3AI GATC 1 cut(s) 34
Sau96I GGNCC 3 cut(s) 107, 412, 1294
SchI GAGTC 2 cut(s) 65, 511
ScrFI CCNGG 1 cut(s) 223
SduI GDGCHC 4 cut(s) 265, 551, 592, 982
SfaNI GCATC 1 cut(s) 804
SfcI CTRYAG 2 cut(s) 927, 1029
SfuI TTCGAA 2 cut(s) 75, 704
SinI GGWCC 1 cut(s) 107
SmiMI CAYNNNNRTG 2 cut(s) 177, 1115
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
Sse9I AATT 8 cut(s) 426, 446, 720, 834, 1245, 1251, 1335, 1366
SsiI CCGC 2 cut(s) 333, 392
SspMI CTAG 8 cut(s) 248, 593, 956, 1127, 1148, 1182, 1283, 1398
SstI GAGCTC 1 cut(s) 551
StyD4I CCNGG 1 cut(s) 221
StyI CCWWGG 5 cut(s) 175, 204, 619, 739, 1116
TaqI TCGA 4 cut(s) 75, 155, 704, 1422
TasI AATT 8 cut(s) 426, 446, 720, 834, 1245, 1251, 1335, 1366
TatI WGTACW 3 cut(s) 135, 667, 1361
TfiI GAWTC 1 cut(s) 1308
TscAI CASTG 2 cut(s) 205, 265
TseFI GTSAC 5 cut(s) 170, 226, 892, 1108, 1351
TseI GCWGC 8 cut(s) 923, 926, 929, 998, 1198, 1226, 1256, 1438
Tsp45I GTSAC 5 cut(s) 170, 226, 892, 1108, 1351
TspDTI ATGAA 4 cut(s) 397, 450, 670, 698
TspRI CASTG 2 cut(s) 205, 265
Van91I CCANNNNNTGG 1 cut(s) 139
VneI GTGCAC 1 cut(s) 261
VpaK11BI GGWCC 1 cut(s) 107
XapI RAATTY 3 cut(s) 446, 834, 1245
XceI RCATGY 1 cut(s) 996
XcmI CCANNNNNNNNNTGG 1 cut(s) 202
XspI CTAG 8 cut(s) 248, 593, 956, 1127, 1148, 1182, 1283, 1398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.