Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 1005 |
| AccB1I | GGYRCC | 1 cut(s) | 1132 |
| AccBSI | CCGCTC | 1 cut(s) | 482 |
| AccII | CGCG | 1 cut(s) | 447 |
| AciI | CCGC | 1 cut(s) | 480 |
| AclWI | GGATC | 3 cut(s) | 35, 425, 1435 |
| AcoI | YGGCCR | 1 cut(s) | 267 |
| AcsI | RAATTY | 3 cut(s) | 50, 458, 1257 |
| AcuI | CTGAAG | 1 cut(s) | 774 |
| AfaI | GTAC | 5 cut(s) | 61, 692, 949, 1158, 1375 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 637, 791 |
| AgsI | TTSAA | 6 cut(s) | 89, 506, 613, 670, 869, 906 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 626 |
| AhlI | ACTAGT | 2 cut(s) | 62, 1367 |
| AjnI | CCWGG | 1 cut(s) | 1419 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1294, 1326 |
| AleI | CACNNNNGTG | 1 cut(s) | 189 |
| Alw21I | GWGCWC | 3 cut(s) | 277, 730, 994 |
| Alw26I | GTCTC | 5 cut(s) | 377, 661, 896, 1028, 1155 |
| Alw44I | GTGCAC | 1 cut(s) | 273 |
| AlwI | GGATC | 3 cut(s) | 35, 425, 1435 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1268 |
| AoxI | GGCC | 2 cut(s) | 267, 1306 |
| ApaLI | GTGCAC | 1 cut(s) | 273 |
| ApeKI | GCWGC | 6 cut(s) | 826, 938, 941, 1001, 1210, 1268 |
| ApoI | RAATTY | 3 cut(s) | 50, 458, 1257 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 520, 552 |
| AspLEI | GCGC | 1 cut(s) | 1006 |
| AspS9I | GGNCC | 2 cut(s) | 119, 1177 |
| AsuHPI | GGTGA | 2 cut(s) | 203, 232 |
| AvaII | GGWCC | 2 cut(s) | 119, 1177 |
| BaeGI | GKGCMC | 1 cut(s) | 277 |
| BalI | TGGCCA | 1 cut(s) | 269 |
| BanI | GGYRCC | 1 cut(s) | 1132 |
| BanII | GRGCYC | 1 cut(s) | 730 |
| Bbv12I | GWGCWC | 3 cut(s) | 277, 730, 994 |
| BbvI | GCAGC | 6 cut(s) | 813, 925, 953, 988, 1222, 1255 |
| BccI | CCATC | 4 cut(s) | 169, 230, 788, 1405 |
| BceAI | ACGGC | 1 cut(s) | 116 |
| BciT130I | CCWGG | 1 cut(s) | 1421 |
| BcoDI | GTCTC | 5 cut(s) | 377, 661, 896, 1028, 1155 |
| BcuI | ACTAGT | 2 cut(s) | 62, 1367 |
| BfaI | CTAG | 8 cut(s) | 63, 207, 605, 951, 1139, 1160, 1194, 1368 |
| BfmI | CTRYAG | 2 cut(s) | 348, 939 |
| BisI | GCNGC | 6 cut(s) | 827, 939, 942, 1002, 1211, 1269 |
| BlpI | GCTNAGC | 1 cut(s) | 309 |
| BlsI | GCNGC | 6 cut(s) | 828, 940, 943, 1003, 1212, 1270 |
| BmcAI | AGTACT | 2 cut(s) | 61, 949 |
| Bme1390I | CCNGG | 1 cut(s) | 1421 |
| Bme18I | GGWCC | 2 cut(s) | 119, 1177 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 626 |
| BmgT120I | GGNCC | 2 cut(s) | 119, 1177 |
| BmiI | GGNNCC | 2 cut(s) | 1134, 1178 |
| BmrFI | CCNGG | 1 cut(s) | 1421 |
| BmrI | ACTGGG | 1 cut(s) | 977 |
| BmuI | ACTGGG | 1 cut(s) | 977 |
| BpmI | CTGGAG | 3 cut(s) | 255, 714, 878 |
| Bpu10I | CCTNAGC | 1 cut(s) | 557 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 309 |
| BsaJI | CCNNGG | 3 cut(s) | 631, 751, 1420 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 315, 345, 712, 742 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 637, 791 |
| Bse118I | RCCGGY | 1 cut(s) | 242 |
| Bse1I | ACTGG | 6 cut(s) | 270, 282, 627, 731, 972, 1262 |
| Bse3DI | GCAATG | 2 cut(s) | 110, 216 |
| BseBI | CCWGG | 1 cut(s) | 1421 |
| BseDI | CCNNGG | 3 cut(s) | 631, 751, 1420 |
| BseGI | GGATG | 3 cut(s) | 631, 780, 1205 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 637, 791 |
| BseMI | GCAATG | 2 cut(s) | 110, 216 |
| BseMII | CTCAG | 2 cut(s) | 996, 1286 |
| BseNI | ACTGG | 6 cut(s) | 270, 282, 627, 731, 972, 1262 |
| BseSI | GKGCMC | 1 cut(s) | 277 |
| BseXI | GCAGC | 6 cut(s) | 813, 925, 953, 988, 1222, 1255 |
| BsgI | GTGCAG | 1 cut(s) | 1361 |
| Bsh1236I | CGCG | 1 cut(s) | 447 |
| BshFI | GGCC | 2 cut(s) | 269, 1308 |
| BshNI | GGYRCC | 1 cut(s) | 1132 |
| BsiHKAI | GWGCWC | 3 cut(s) | 277, 730, 994 |
| BsiSI | CCGG | 2 cut(s) | 243, 1069 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 637, 791 |
| BsmAI | GTCTC | 5 cut(s) | 377, 661, 896, 1028, 1155 |
| BsnI | GGCC | 2 cut(s) | 269, 1308 |
| Bsp1286I | GDGCHC | 3 cut(s) | 277, 730, 994 |
| Bsp1407I | TGTACA | 1 cut(s) | 690 |
| Bsp143I | GATC | 3 cut(s) | 40, 430, 1427 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 309 |
| Bsp19I | CCATGG | 1 cut(s) | 751 |
| Bsp68I | TCGCGA | 1 cut(s) | 447 |
| BspACI | CCGC | 1 cut(s) | 480 |
| BspANI | GGCC | 2 cut(s) | 269, 1308 |
| BspCNI | CTCAG | 2 cut(s) | 995, 1287 |
| BspFNI | CGCG | 1 cut(s) | 447 |
| BspLI | GGNNCC | 2 cut(s) | 1134, 1178 |
| BspMAI | CTGCAG | 1 cut(s) | 943 |
| BspPI | GGATC | 3 cut(s) | 35, 425, 1435 |
| BspT107I | GGYRCC | 1 cut(s) | 1132 |
| BsrBI | CCGCTC | 1 cut(s) | 482 |
| BsrDI | GCAATG | 2 cut(s) | 110, 216 |
| BsrFI | RCCGGY | 1 cut(s) | 242 |
| BsrGI | TGTACA | 1 cut(s) | 690 |
| BsrI | ACTGG | 6 cut(s) | 270, 282, 627, 731, 972, 1262 |
| BssAI | RCCGGY | 1 cut(s) | 242 |
| BssECI | CCNNGG | 3 cut(s) | 631, 751, 1420 |
| BssMI | GATC | 3 cut(s) | 40, 430, 1427 |
| BssT1I | CCWWGG | 2 cut(s) | 631, 751 |
| Bst2UI | CCWGG | 1 cut(s) | 1421 |
| Bst6I | CTCTTC | 3 cut(s) | 92, 507, 1297 |
| BstAUI | TGTACA | 1 cut(s) | 690 |
| BstC8I | GCNNGC | 5 cut(s) | 198, 297, 454, 1169, 1392 |
| BstDEI | CTNAG | 5 cut(s) | 309, 557, 738, 982, 1295 |
| BstDSI | CCRYGG | 1 cut(s) | 751 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 789 |
| BstF5I | GGATG | 3 cut(s) | 631, 780, 1205 |
| BstFNI | CGCG | 1 cut(s) | 447 |
| BstHHI | GCGC | 1 cut(s) | 1006 |
| BstKTI | GATC | 3 cut(s) | 43, 433, 1430 |
| BstMAI | GTCTC | 5 cut(s) | 377, 661, 896, 1028, 1155 |
| BstMBI | GATC | 3 cut(s) | 40, 430, 1427 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 305, 453, 488, 835, 844, 998, 1010, 1277, 1400 |
| BstNI | CCWGG | 1 cut(s) | 1421 |
| BstNSI | RCATGY | 1 cut(s) | 1010 |
| BstSCI | CCNGG | 1 cut(s) | 1419 |
| BstSFI | CTRYAG | 2 cut(s) | 348, 939 |
| BstSLI | GKGCMC | 1 cut(s) | 277 |
| BstUI | CGCG | 1 cut(s) | 447 |
| BstV1I | GCAGC | 6 cut(s) | 813, 925, 953, 988, 1222, 1255 |
| BstX2I | RGATCY | 2 cut(s) | 40, 430 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 277 |
| BstYI | RGATCY | 2 cut(s) | 40, 430 |
| BsuRI | GGCC | 2 cut(s) | 269, 1308 |
| BtgI | CCRYGG | 1 cut(s) | 751 |
| BtsCI | GGATG | 3 cut(s) | 631, 780, 1205 |
| BtsI | GCAGTG | 1 cut(s) | 342 |
| BtsIMutI | CAGTG | 3 cut(s) | 275, 277, 342 |
| BtuMI | TCGCGA | 1 cut(s) | 447 |
| Cac8I | GCNNGC | 5 cut(s) | 198, 297, 454, 1169, 1392 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1268 |
| CfoI | GCGC | 1 cut(s) | 1006 |
| Cfr10I | RCCGGY | 1 cut(s) | 242 |
| Cfr13I | GGNCC | 2 cut(s) | 119, 1177 |
| Csp6I | GTAC | 5 cut(s) | 60, 691, 948, 1157, 1374 |
| CviAII | CATG | 5 cut(s) | 72, 421, 694, 752, 1007 |
| CviQI | GTAC | 5 cut(s) | 60, 691, 948, 1157, 1374 |
| DdeI | CTNAG | 5 cut(s) | 309, 557, 738, 982, 1295 |
| DpnI | GATC | 3 cut(s) | 42, 432, 1429 |
| DpnII | GATC | 3 cut(s) | 40, 430, 1427 |
| DriI | GACNNNNNGTC | 1 cut(s) | 626 |
| EaeI | YGGCCR | 1 cut(s) | 267 |
| Eam1104I | CTCTTC | 3 cut(s) | 92, 507, 1297 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 626 |
| EarI | CTCTTC | 3 cut(s) | 92, 507, 1297 |
| Ecl136II | GAGCTC | 1 cut(s) | 728 |
| Eco130I | CCWWGG | 2 cut(s) | 631, 751 |
| Eco147I | AGGCCT | 1 cut(s) | 1308 |
| Eco24I | GRGCYC | 1 cut(s) | 730 |
| Eco47I | GGWCC | 2 cut(s) | 119, 1177 |
| Eco53kI | GAGCTC | 1 cut(s) | 728 |
| Eco57I | CTGAAG | 1 cut(s) | 774 |
| EcoICRI | GAGCTC | 1 cut(s) | 728 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 789 |
| EcoO109I | RGGNCCY | 2 cut(s) | 119, 1177 |
| EcoRI | GAATTC | 1 cut(s) | 1257 |
| EcoRII | CCWGG | 1 cut(s) | 1419 |
| EcoT14I | CCWWGG | 2 cut(s) | 631, 751 |
| EcoT22I | ATGCAT | 1 cut(s) | 806 |
| EcoT38I | GRGCYC | 1 cut(s) | 730 |
| ErhI | CCWWGG | 2 cut(s) | 631, 751 |
| FaeI | CATG | 5 cut(s) | 75, 424, 697, 755, 1010 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1164, 1196 |
| FatI | CATG | 5 cut(s) | 71, 420, 693, 751, 1006 |
| FauI | CCCGC | 1 cut(s) | 487 |
| Fnu4HI | GCNGC | 6 cut(s) | 827, 939, 942, 1002, 1211, 1269 |
| FokI | GGATG | 3 cut(s) | 638, 767, 1212 |
| FriOI | GRGCYC | 1 cut(s) | 730 |
| Fsp4HI | GCNGC | 6 cut(s) | 827, 939, 942, 1002, 1211, 1269 |
| FspBI | CTAG | 8 cut(s) | 63, 207, 605, 951, 1139, 1160, 1194, 1368 |
| FspI | TGCGCA | 1 cut(s) | 1005 |
| GlaI | GCGC | 1 cut(s) | 1005 |
| GluI | GCNGC | 6 cut(s) | 827, 939, 942, 1002, 1211, 1269 |
| GsuI | CTGGAG | 3 cut(s) | 255, 714, 878 |
| HaeIII | GGCC | 2 cut(s) | 269, 1308 |
| HapII | CCGG | 2 cut(s) | 243, 1069 |
| HhaI | GCGC | 1 cut(s) | 1006 |
| Hin1II | CATG | 5 cut(s) | 75, 424, 697, 755, 1010 |
| Hin6I | GCGC | 1 cut(s) | 1004 |
| HinP1I | GCGC | 1 cut(s) | 1004 |
| HincII | GTYRAC | 1 cut(s) | 18 |
| HindII | GTYRAC | 1 cut(s) | 18 |
| HinfI | GANTC | 5 cut(s) | 136, 237, 330, 475, 544 |
| HpaI | GTTAAC | 1 cut(s) | 18 |
| HpaII | CCGG | 2 cut(s) | 243, 1069 |
| HphI | GGTGA | 2 cut(s) | 203, 232 |
| Hpy166II | GTNNAC | 3 cut(s) | 18, 275, 716 |
| Hpy188I | TCNGA | 5 cut(s) | 321, 518, 528, 985, 1435 |
| Hpy188III | TCNNGA | 7 cut(s) | 234, 428, 446, 618, 792, 866, 895 |
| Hpy8I | GTNNAC | 3 cut(s) | 18, 275, 716 |
| Hpy99I | CGWCG | 1 cut(s) | 1115 |
| HpyAV | CCTTC | 2 cut(s) | 795, 1075 |
| HpyCH4IV | ACGT | 1 cut(s) | 1119 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 305, 453, 488, 835, 844, 998, 1010, 1277, 1400 |
| HpyF3I | CTNAG | 5 cut(s) | 309, 557, 738, 982, 1295 |
| HpySE526I | ACGT | 1 cut(s) | 1119 |
| Hsp92II | CATG | 5 cut(s) | 75, 424, 697, 755, 1010 |
| HspAI | GCGC | 1 cut(s) | 1004 |
| KspAI | GTTAAC | 1 cut(s) | 18 |
| Kzo9I | GATC | 3 cut(s) | 40, 430, 1427 |
| LmnI | GCTCC | 5 cut(s) | 305, 558, 733, 971, 1238 |
| Lsp1109I | GCAGC | 6 cut(s) | 813, 925, 953, 988, 1222, 1255 |
| MaeI | CTAG | 8 cut(s) | 63, 207, 605, 951, 1139, 1160, 1194, 1368 |
| MaeII | ACGT | 1 cut(s) | 1119 |
| MaeIII | GTNAC | 8 cut(s) | 107, 182, 238, 977, 1027, 1082, 1120, 1363 |
| MalI | GATC | 3 cut(s) | 42, 432, 1429 |
| MbiI | CCGCTC | 1 cut(s) | 482 |
| MboI | GATC | 3 cut(s) | 40, 430, 1427 |
| MboII | GAAGA | 8 cut(s) | 17, 101, 109, 494, 696, 780, 1314, 1448 |
| MflI | RGATCY | 2 cut(s) | 40, 430 |
| MhlI | GDGCHC | 3 cut(s) | 277, 730, 994 |
| MlsI | TGGCCA | 1 cut(s) | 269 |
| MluCI | AATT | 6 cut(s) | 50, 366, 458, 465, 1257, 1378 |
| MluNI | TGGCCA | 1 cut(s) | 269 |
| MlyI | GAGTC | 3 cut(s) | 246, 324, 538 |
| Mox20I | TGGCCA | 1 cut(s) | 269 |
| Mph1103I | ATGCAT | 1 cut(s) | 806 |
| MscI | TGGCCA | 1 cut(s) | 269 |
| MseI | TTAA | 2 cut(s) | 17, 365 |
| MslI | CAYNNNNRTG | 2 cut(s) | 189, 1127 |
| Msp20I | TGGCCA | 1 cut(s) | 269 |
| MspI | CCGG | 2 cut(s) | 243, 1069 |
| MspR9I | CCNGG | 1 cut(s) | 1421 |
| MvaI | CCWGG | 1 cut(s) | 1421 |
| MvnI | CGCG | 1 cut(s) | 447 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 305, 453, 488, 835, 844, 998, 1010, 1277, 1400 |
| NcoI | CCATGG | 1 cut(s) | 751 |
| NdeII | GATC | 3 cut(s) | 40, 430, 1427 |
| NlaIII | CATG | 5 cut(s) | 75, 424, 697, 755, 1010 |
| NlaIV | GGNNCC | 2 cut(s) | 1134, 1178 |
| NmuCI | GTSAC | 4 cut(s) | 182, 238, 1027, 1120 |
| NruI | TCGCGA | 1 cut(s) | 447 |
| NsbI | TGCGCA | 1 cut(s) | 1005 |
| NsiI | ATGCAT | 1 cut(s) | 806 |
| NspI | RCATGY | 1 cut(s) | 1010 |
| OliI | CACNNNNGTG | 1 cut(s) | 189 |
| PceI | AGGCCT | 1 cut(s) | 1308 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1116 |
| PfeI | GAWTC | 2 cut(s) | 136, 475 |
| PkrI | GCNGC | 6 cut(s) | 828, 940, 943, 1003, 1212, 1270 |
| PleI | GAGTC | 3 cut(s) | 245, 324, 538 |
| PpsI | GAGTC | 3 cut(s) | 245, 324, 538 |
| PpuMI | RGGWCCY | 2 cut(s) | 119, 1177 |
| Psp124BI | GAGCTC | 1 cut(s) | 730 |
| Psp5II | RGGWCCY | 2 cut(s) | 119, 1177 |
| Psp6I | CCWGG | 1 cut(s) | 1419 |
| PspGI | CCWGG | 1 cut(s) | 1419 |
| PspN4I | GGNNCC | 2 cut(s) | 1134, 1178 |
| PspPI | GGNCC | 2 cut(s) | 119, 1177 |
| PspPPI | RGGWCCY | 2 cut(s) | 119, 1177 |
| PstI | CTGCAG | 1 cut(s) | 943 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1268 |
| PsuI | RGATCY | 2 cut(s) | 40, 430 |
| RruI | TCGCGA | 1 cut(s) | 447 |
| RsaI | GTAC | 5 cut(s) | 61, 692, 949, 1158, 1375 |
| RsaNI | GTAC | 5 cut(s) | 60, 691, 948, 1157, 1374 |
| RseI | CAYNNNNRTG | 2 cut(s) | 189, 1127 |
| SacI | GAGCTC | 1 cut(s) | 730 |
| SaqAI | TTAA | 2 cut(s) | 17, 365 |
| SatI | GCNGC | 6 cut(s) | 827, 939, 942, 1002, 1211, 1269 |
| Sau3AI | GATC | 3 cut(s) | 40, 430, 1427 |
| Sau96I | GGNCC | 2 cut(s) | 119, 1177 |
| ScaI | AGTACT | 2 cut(s) | 61, 949 |
| SchI | GAGTC | 3 cut(s) | 246, 324, 538 |
| ScrFI | CCNGG | 1 cut(s) | 1421 |
| SduI | GDGCHC | 3 cut(s) | 277, 730, 994 |
| SfcI | CTRYAG | 2 cut(s) | 348, 939 |
| SinI | GGWCC | 2 cut(s) | 119, 1177 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 189, 1127 |
| SpeI | ACTAGT | 2 cut(s) | 62, 1367 |
| Sse9I | AATT | 6 cut(s) | 50, 366, 458, 465, 1257, 1378 |
| SseBI | AGGCCT | 1 cut(s) | 1308 |
| SsiI | CCGC | 1 cut(s) | 480 |
| SspMI | CTAG | 8 cut(s) | 63, 207, 605, 951, 1139, 1160, 1194, 1368 |
| SstI | GAGCTC | 1 cut(s) | 730 |
| StuI | AGGCCT | 1 cut(s) | 1308 |
| StyD4I | CCNGG | 1 cut(s) | 1419 |
| StyI | CCWWGG | 2 cut(s) | 631, 751 |
| TaiI | ACGT | 1 cut(s) | 1122 |
| TaqI | TCGA | 2 cut(s) | 167, 1113 |
| TasI | AATT | 6 cut(s) | 50, 366, 458, 465, 1257, 1378 |
| TatI | WGTACW | 5 cut(s) | 59, 690, 947, 1156, 1373 |
| TfiI | GAWTC | 2 cut(s) | 136, 475 |
| Tru1I | TTAA | 2 cut(s) | 17, 365 |
| Tru9I | TTAA | 2 cut(s) | 17, 365 |
| TscAI | CASTG | 3 cut(s) | 277, 282, 349 |
| TseFI | GTSAC | 4 cut(s) | 182, 238, 1027, 1120 |
| TseI | GCWGC | 6 cut(s) | 826, 938, 941, 1001, 1210, 1268 |
| Tsp45I | GTSAC | 4 cut(s) | 182, 238, 1027, 1120 |
| TspDTI | ATGAA | 3 cut(s) | 409, 710, 978 |
| TspRI | CASTG | 3 cut(s) | 277, 282, 349 |
| VneI | GTGCAC | 1 cut(s) | 273 |
| VpaK11BI | GGWCC | 2 cut(s) | 119, 1177 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 789 |
| XapI | RAATTY | 3 cut(s) | 50, 458, 1257 |
| XceI | RCATGY | 1 cut(s) | 1010 |
| XspI | CTAG | 8 cut(s) | 63, 207, 605, 951, 1139, 1160, 1194, 1368 |
| ZrmI | AGTACT | 2 cut(s) | 61, 949 |
| Zsp2I | ATGCAT | 1 cut(s) | 806 |