Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0002865

Physical Location & Seq

Genomic Coordinates Reverse (-)

50810420 .. 50836337

Gene Structure 25918 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000009312

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3711 bp

ATGGAGCTGCTGCCATGGAAACTATGTAAGGATGCAGGCAAGTTCAACTTGGACGCCAAGTGCTCGTCGGCCAATATGGCCATCCATCCGGTCCTCCTGTTGATCATGTTTCCAACTTTTGAATGCCGAGACATTCAAGCCAGTAGCGGAAGTGACAGTTATCTGATATATGTCCATTGGAGAGAGAGCTACGAAATGCACCTTCACAATCTGAAACGATATTGTAGCTGCCAACCACGCCTTTACCTCGAGGGCAAGAAAAGATTGGTTAGGCTTGAAGTTCTGCGGAGACCTAAATCTCAATTTTACTTGGAGGTAACTTTCTCCACACTCCCAAGGGGTGTTACTTCACAATCCAAGGAAAATTACTTCTCAAAGCATAAGAAATATGTGTACTTCTCTCGAGATCCTCATGACAAGGCACTTTTCTTGCAATCTGTTGGAAAGATCGTCCCTCGCATTGACAAATTTTCTAGATCTTCCCTCGCTTCACACAAAGAAGAATATAATGGAGGAAAAGACAATGGTCAAGGTGATGTCATGGTCTCATGGATAAGAGACAGGCATCAGTTGCATTCATATCGGTGTTCTTCGTCTGGAGAGGAAACTACAACGACGGCTCAGAGAAGGATGAATCAGTCAAGTGGGTTGCCTTGTAACCCATGCAATGATGACTTGGAGATCGGTCTGAAGGAGGATCATGTTGAGCGCAGGAACCGGTGGACGCAAGCGAAGCTAAGTCTGAAAGCATTGTACCGATTCCACCACACACTTGTCCTGAAAAGGGCAGAAGAAAAGAAGCAGGCAGCAAGGAAACTAATTAGAGCACATACCCAAGCCCTACGAGCTGCGGATATCTTCAAAGAAGTTGCAGATCAAGTGAAAGGGACCGGAGATTGTTTTCCAATTGAACCAGATGAACTTGCTAGTTTGACGAGGAATAGTGCTGTTGATGCTCTGCAGCAATATGGGGGGAAGGAAGGCTTGGCAGATTTACTGAAAACAGATTTAGACAAGGGAATTGATGGGGATGATTTACTAAAGCGGAGAATTAAATTTGGATCAAACATATATCCTCCAAAAACAGCAACGAGCCACTGGACATGTCTTCGGGAAGCTCTGTACGATTTTACTCGGATCGTTTTGATGATTGCTTTTATGGGTTCTGTTGTGATGGGTATTCTGGAAGAGACTAAAAAGCTGGGATGGCACGATGTGTTCAGCATTGCTATTGCAGCTGCCATGTTCTTTCTTATCACAGTCTTACCTGAGTATATTCAAGCTTGTCAGTTCCAGCATATGATTATCAGGGAGGAAATCAGAAACATAAAATTGAAGGTTATTAGAGCTGGTCGACAAGTTCCAGTTTCTATTTGTGATATTGTTGTTGGTGATGTGATATTCCTTGAGTTTGGTGATGAGGTCCCTGCTGATGGCATTTTAATCCGTGGTTGCTCACTTGCAATTGATAAGAGTAGAGTCACTGGAGAGAGCAAGACTTTTCACAAGGATTCAAGGAACCCATTTCTACGGTCTGGCTGGAAAGTAGCACATGGCAGTGCTACTATGCTGGTAACTGGGGTTGGGAAATGTACTGAATCAGGAATTCTCAGGGCTAACACTTATTCAAAAGACACCGAGAATAGAACCCCCTTGCAGGTACTTTTAAAGGAGGTTGCTACTTTCATGGGTGCTGTCGGGTTCATGGGTGCTGCCATTGTTGCATATGTTGTTTGGTTCAGAGACTTCTTTTCTGGTTATAATTCAAATGGAAGTCCCGAACCTGGAAGTATACTTGAAGAAGTTATTAGAATTAGTATCATTGCAGTTGCCATTTTTGTCGTTGCATTACCCGTAGTGCTTCCTTCAGCTTTCAACATAAGCCGTCTCAGCACAATGAAAAAGATGAAGGCAGACAATGCCTTGGTGCGGAGGCCAGCTGTGTGTGAAACCATGGCCTTATGCAATGATGCACTTTGTGACAAGACTGGAATAACTTTGAACCAGATGACTGTTTCTGAGGCCTATGCTGGTCAGAAGAAAATCAATTTTGCTGACAGCACCAGCAACTTGCCTCCAATACTATCGTCCTTGCTTGTTGAAGGAATTGCTCAGAATACGAGTGGCTGTCTATCTGTGCTTGGGAGTGGTGATGTTCAGGCTTCTGGATCACCAATTGATAGGGCAATTCTGCATTGGGGAGTCAAGCTGGGAATGAATTTTGATGACATAACGTCTCAGTCTCCAGTTATGCAAGTTTTGCCATTCAGTTCAGAGAAAAAACGAGGTGGTGTTGCACTTAATCTGACACACGGTCGAGTTCATGTACACTGGAAAGGGGAAGCTCAAGGAGTCTTAGCCTCATGTACAAGATACATTGGGGCTAATGATCAGGTTTTACCAATGGATGATGATGAGTTAACATTTTTTCAAAAAGCTATCGAGGATATGGCTTCTGGAGGTTTGCAATGCGTTGCTATTGCATATAGATCACTTGAATTGGAAAATGTTCTAATCAATAGTAATGGACAACTAGCGCGGTGGGAACTACCTGAGGATGACCTCATTTTACTAGCTATTGTTGGTATTAAGGATGATTGTCAACCGGGGATCAAGGACTCAGTGGAAAAATTGCAAAGAGCTGGCATTAAGGTACGCCTTGTTACGCATGACAATCCTCAAACTGCAAAAACAATTGCTAAGGAATGTGGCATACTTGCTTCTGATTGGTCAGATGCGAGTGAGGAAAATCTTATTGAAGGAAAAGTGTTCCGAGAACGTTCTGATAGTCAGAGAGAAGGATTAGCAGAGAATATTTTGGTAATGGCACGGGCTTCCCCCAATGACAAGCGTTTGCTTGTGCGAGCACTGCAGAAAAGAGGAAAGGTTGTTGCTGTCACTGGATATGTCAGTAATGATGCTTATGCACTACATGAGGCCGACATTGGTCTTGCTATGGGTATTCAAGGGACTGAAGTTGTTAAAGAGAGTTCAGATATCATAATTTCGGATGACAATTTTGCTACAGTATACAAGTGTTTCAGATGGGGACGATCTATGCATAAAAAGGTTCTGAAAGTTATGCTGCAGCAACTTACAGCCAATATTGTAGCAGTAACCATAAATTGCATGGCGGCAGTTTACTATCGTTACATCCCGCTCAATGCTGTGCAGTTTGTGTGGGTGAATCTTATCATATTTGGTCTTGGGCCACTAGCATTGGCTACTGAATCTCCGGCTGATCATCTGATGAGCGTACCCCCTATTGACCAAGGACAAACTCTTATAAAATATATTCAGTGGAAGAGACTGTTGATACAGGTTCTATACCAAATTATAGCGCTGCTTGTCATCAATTTTCAAGGAGGGAGTTTACTGAAAAATTATACCAGAGACCACGCCACAAAAGTAAAGAATACAATGATATTCAATACATTTGTTGTTTGTCAGATTTCCATTGAATTCGGAGTTCGAAAGACTGGTATAGTGGACCATCCTTTGAAATGGATTATTGGTAACTCTGTGTTTCTGGGAATAGTTGGAATAATTCTTATGATTCAGTTTATCATCATTGAGTTTCTTGGAAAGGCATTTTTCATAGTCAGGCTTGACTGGAAAGAGTGGCAGTTTTCGCTGACTCTTGGTATTGTTGAGTTGTTCTTTGCACTGCTTCTATTTCTGAAGCACTTGACACAGAGGAATCATAATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009506 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1237

Amino Acids

138.2

Weight (kDa)

8.79

Isoelectric Point (pI)

38.26

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	323 - 385	2.9e-09	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	444 - 535	1.1e-15	P-type ATPase actuator domain
Cation_ATPase	PF13246	699 - 869	8.3e-25	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	856 - 981	4.2e-07	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	1052 - 1222	8.8e-24	Cation transporting ATPase, C-terminus

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0002865)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g06912
pyrus_communis	pycom14g06520
rosa_chinensis	RchiOBHm_Chr4g0398821 RchiOBHm_Chr4g0398831 RchiOBHm_Chr4g0398851 RchiOBHm_Chr4g0398861
rosa_laevigata	RLG00000009311 RLG00000009312 RLG00000009316
rosa_multiflora	Rmu_sc0002885.1_g000007 Rmu_sc0002885.1_g000009 Rmu_sc0004474.1_g000001 Rmu_sc0004751.1_g000005
rosa_roxburghii	Rroxscaffold_173G00435010 Rroxscaffold_5G00343860 Rroxscaffold_5G00343900 Rroxscaffold_5G00355000
rosa_rugosa	Rorug04G0012100 Rorug04G0012300 Rorug04G0012400 Rorug04G0012600
rosa_samantha	Rh4AG090100 Rh4AG090400 Rh4BG087200 Rh4BG087500 Rh4BG087600 Rh4BG087700 Rh4CG098200 Rh4CG098400 Rh4CG098700 Rh4DG081500 Rh4DG081600 Rh4DG081800 Rh4DG082000 Rh4DG082300 Rh4DG082700
rosa_wichuraiana	Rw4G007420 Rw4G007440 Rw4G007480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1761, 3287
AbsI	CCTCGAGG	1 cut(s)	248
Acc36I	ACCTGC	1 cut(s)	1648
AccBSI	CCGCTC	1 cut(s)	3160
AccI	GTMKAC	3 cut(s)	1354, 1792, 3030
AccII	CGCG	1 cut(s)	2539
AciI	CCGC	8 cut(s)	147, 286, 851, 1045, 1930, 2539, 3134, 3158
AclI	AACGTT	1 cut(s)	2779
AclWI	GGATC	6 cut(s)	401, 705, 1069, 1145, 2176, 2618
AcoI	YGGCCR	2 cut(s)	69, 78
AcsI	RAATTY	5 cut(s)	467, 1055, 1605, 2218, 3461
AcuI	CTGAAG	4 cut(s)	710, 1851, 2994, 3701
AcyI	GRCGYC	1 cut(s)	54
AdeI	CACNNNGTG	2 cut(s)	1216, 1979
AfaI	GTAC	9 cut(s)	395, 755, 1124, 1594, 1662, 2328, 2368, 2655, 3258
AfeI	AGCGCT	1 cut(s)	3342
AfiI	CCNNNNNNNGG	5 cut(s)	784, 1433, 1657, 1784, 1929
AflIII	ACRYGT	1 cut(s)	1103
AgeI	ACCGGT	1 cut(s)	717
AhdI	GACNNNNNGTC	2 cut(s)	2233, 2313
AjnI	CCWGG	1 cut(s)	1783
AjuI	GAANNNNNNNTTGG	2 cut(s)	968, 1000
Alw21I	GWGCWC	3 cut(s)	65, 829, 2869
AlwI	GGATC	6 cut(s)	401, 705, 1069, 1145, 2176, 2618
Ama87I	CYCGRG	2 cut(s)	248, 402
Aor51HI	AGCGCT	1 cut(s)	3342
AoxI	GGCC	7 cut(s)	69, 78, 1934, 1956, 2022, 2937, 3209
ApoI	RAATTY	5 cut(s)	467, 1055, 1605, 2218, 3461
AsiGI	ACCGGT	1 cut(s)	717
Asp700I	GAANNNNTTC	1 cut(s)	2508
AspLEI	GCGC	3 cut(s)	711, 2539, 3343
AspS9I	GGNCC	5 cut(s)	91, 888, 1423, 3209, 3490
AsuC2I	CCSGG	1 cut(s)	2607
AsuHPI	GGTGA	6 cut(s)	545, 1403, 1427, 2162, 2163, 3196
AsuII	TTCGAA	1 cut(s)	3472
AvaI	CYCGRG	2 cut(s)	248, 402
AvaII	GGWCC	4 cut(s)	91, 888, 1423, 3490
AxyI	CCTNAGG	1 cut(s)	2553
BalI	TGGCCA	1 cut(s)	80
BbsI	GAAGAC	1 cut(s)	1100
Bbv12I	GWGCWC	3 cut(s)	65, 829, 2869
BccI	CCATC	8 cut(s)	89, 93, 1019, 1168, 1200, 1427, 3039, 3501
BceAI	ACGGC	2 cut(s)	633, 1869
BcgI	CGANNNNNNTGC	2 cut(s)	563, 597
BciT130I	CCWGG	1 cut(s)	1785
BclI	TGATCA	3 cut(s)	102, 2389, 3241
BcnI	CCSGG	1 cut(s)	2607
BfaI	CTAG	5 cut(s)	474, 927, 2534, 2573, 3215
BfmI	CTRYAG	4 cut(s)	959, 2870, 3024, 3086
BfoI	RGCGCY	1 cut(s)	3344
BfuAI	ACCTGC	1 cut(s)	1648
BglI	GCCNNNNNGGC	1 cut(s)	77
BglII	AGATCT	1 cut(s)	476
Bme1390I	CCNGG	2 cut(s)	1785, 2607
Bme18I	GGWCC	4 cut(s)	91, 888, 1423, 3490
BmeRI	GACNNNNNGTC	2 cut(s)	2233, 2313
BmeT110I	CYCGRG	2 cut(s)	248, 402
BmgT120I	GGNCC	5 cut(s)	91, 888, 1423, 3209, 3490
BmiI	GGNNCC	4 cut(s)	716, 889, 1425, 1520
BmrFI	CCNGG	2 cut(s)	1785, 2607
BmrI	ACTGGG	1 cut(s)	1587
BmsI	GCATC	6 cut(s)	22, 574, 943, 1960, 2725, 2908
BmuI	ACTGGG	1 cut(s)	1587
BoxI	GACNNNNGTC	2 cut(s)	525, 2946
BpiI	GAAGAC	1 cut(s)	1100
BpmI	CTGGAG	4 cut(s)	618, 1506, 2229, 2478
Bpu10I	CCTNAGC	1 cut(s)	2700
Bpu14I	TTCGAA	1 cut(s)	3472
BpuEI	CTTGAG	2 cut(s)	1427, 2331
BpuMI	CCSGG	1 cut(s)	2607
BsaHI	GRCGYC	1 cut(s)	54
BsaI	GGTCTC	3 cut(s)	283, 550, 3387
BsaJI	CCNNGG	8 cut(s)	14, 335, 357, 1447, 1923, 1953, 2606, 3271
BsaWI	WCCGGW	3 cut(s)	88, 717, 890
BsaXI	ACNNNNNCTCC	2 cut(s)	3217, 3247
Bsc4I	CCNNNNNNNGG	5 cut(s)	784, 1433, 1657, 1784, 1929
Bse118I	RCCGGY	1 cut(s)	717
Bse21I	CCTNAGG	1 cut(s)	2553
Bse3DI	GCAATG	5 cut(s)	673, 1224, 1821, 1972, 2474
BseBI	CCWGG	1 cut(s)	1785
BseDI	CCNNGG	8 cut(s)	14, 335, 357, 1447, 1923, 1953, 2606, 3271
BseLI	CCNNNNNNNGG	5 cut(s)	784, 1433, 1657, 1784, 1929
BseMI	GCAATG	5 cut(s)	673, 1224, 1821, 1972, 2474
BseMII	CTCAG	9 cut(s)	635, 1260, 1624, 1903, 2010, 2126, 2252, 2544, 2634
BseYI	CCCAGC	2 cut(s)	1201, 2209
BsgI	GTGCAG	1 cut(s)	3191
Bsh1236I	CGCG	1 cut(s)	2539
Bsh1285I	CGRYCG	1 cut(s)	2317
BshFI	GGCC	7 cut(s)	71, 80, 1936, 1958, 2024, 2939, 3211
BshTI	ACCGGT	1 cut(s)	717
BsiEI	CGRYCG	1 cut(s)	2317
BsiHKAI	GWGCWC	3 cut(s)	65, 829, 2869
BsiHKCI	CYCGRG	2 cut(s)	248, 402
BsiSI	CCGG	5 cut(s)	89, 718, 891, 2606, 3236
BslFI	GGGAC	6 cut(s)	437, 901, 1409, 1761, 2983, 3063
BslI	CCNNNNNNNGG	5 cut(s)	784, 1433, 1657, 1784, 1929
BsmBI	CGTCTC	2 cut(s)	1892, 2241
BsmFI	GGGAC	6 cut(s)	437, 901, 1409, 1761, 2983, 3063
BsmI	GAATGC	2 cut(s)	128, 574
BsnI	GGCC	7 cut(s)	71, 80, 1936, 1958, 2024, 2939, 3211
Bso31I	GGTCTC	3 cut(s)	283, 550, 3387
BsoBI	CYCGRG	2 cut(s)	248, 402
Bsp119I	TTCGAA	1 cut(s)	3472
Bsp1286I	GDGCHC	3 cut(s)	65, 829, 2869
Bsp1407I	TGTACA	2 cut(s)	2326, 2366
Bsp19I	CCATGG	2 cut(s)	14, 1953
BspACI	CCGC	8 cut(s)	147, 286, 851, 1045, 1930, 2539, 3134, 3158
BspANI	GGCC	7 cut(s)	71, 80, 1936, 1958, 2024, 2939, 3211
BspCNI	CTCAG	9 cut(s)	634, 1261, 1623, 1902, 2011, 2125, 2251, 2545, 2633
BspFNI	CGCG	1 cut(s)	2539
BspHI	TCATGA	1 cut(s)	412
BspLI	GGNNCC	4 cut(s)	716, 889, 1425, 1520
BspMAI	CTGCAG	3 cut(s)	963, 2874, 3090
BspMI	ACCTGC	1 cut(s)	1648
BspPI	GGATC	6 cut(s)	401, 705, 1069, 1145, 2176, 2618
BspT104I	TTCGAA	1 cut(s)	3472
BspTNI	GGTCTC	3 cut(s)	283, 550, 3387
BsrBI	CCGCTC	1 cut(s)	3160
BsrDI	GCAATG	5 cut(s)	673, 1224, 1821, 1972, 2474
BsrFI	RCCGGY	1 cut(s)	717
BsrGI	TGTACA	2 cut(s)	2326, 2366
BssAI	RCCGGY	1 cut(s)	717
BssECI	CCNNGG	8 cut(s)	14, 335, 357, 1447, 1923, 1953, 2606, 3271
BssNAI	GTATAC	2 cut(s)	1793, 3031
BssNI	GRCGYC	1 cut(s)	54
BssT1I	CCWWGG	6 cut(s)	14, 335, 357, 1923, 1953, 3271
Bst1107I	GTATAC	2 cut(s)	1793, 3031
Bst2UI	CCWGG	1 cut(s)	1785
Bst4CI	ACNGT	7 cut(s)	158, 1261, 1533, 2014, 2315, 3028, 3312
Bst6I	CTCTTC	2 cut(s)	1182, 3299
BstACI	GRCGYC	1 cut(s)	54
BstAPI	GCANNNNNTGC	3 cut(s)	571, 1919, 2260
BstAUI	TGTACA	2 cut(s)	2326, 2366
BstBI	TTCGAA	1 cut(s)	3472
BstC8I	GCNNGC	6 cut(s)	37, 729, 804, 1938, 2644, 2865
BstDSI	CCRYGG	3 cut(s)	14, 1447, 1953
BstFNI	CGCG	1 cut(s)	2539
BstH2I	RGCGCY	1 cut(s)	3344
BstHHI	GCGC	3 cut(s)	711, 2539, 3343
BstMCI	CGRYCG	1 cut(s)	2317
BstNI	CCWGG	1 cut(s)	1785
BstNSI	RCATGY	1 cut(s)	1107
BstPAI	GACNNNNGTC	2 cut(s)	525, 2946
BstSCI	CCNGG	2 cut(s)	1783, 2605
BstSFI	CTRYAG	4 cut(s)	959, 2870, 3024, 3086
BstUI	CGCG	1 cut(s)	2539
BstV2I	GAAGAC	1 cut(s)	1100
BstX2I	RGATCY	2 cut(s)	406, 476
BstYI	RGATCY	2 cut(s)	406, 476
BstZ17I	GTATAC	2 cut(s)	1793, 3031
Bsu36I	CCTNAGG	1 cut(s)	2553
BsuRI	GGCC	7 cut(s)	71, 80, 1936, 1958, 2024, 2939, 3211
BtgI	CCRYGG	3 cut(s)	14, 1447, 1953
BtsI	GCAGTG	3 cut(s)	1564, 2867, 3665
BtsIMutI	CAGTG	9 cut(s)	1096, 1482, 1564, 2329, 2628, 2867, 2898, 3305, 3665
BveI	ACCTGC	1 cut(s)	1648
Cac8I	GCNNGC	6 cut(s)	37, 729, 804, 1938, 2644, 2865
CciI	TCATGA	1 cut(s)	412
CfoI	GCGC	3 cut(s)	711, 2539, 3343
Cfr10I	RCCGGY	1 cut(s)	717
Cfr13I	GGNCC	5 cut(s)	91, 888, 1423, 3209, 3490
CseI	GACGC	2 cut(s)	62, 733
Csp6I	GTAC	9 cut(s)	394, 754, 1123, 1593, 1661, 2327, 2367, 2654, 3257
CspAI	ACCGGT	1 cut(s)	717
CspCI	CAANNNNNGTGG	2 cut(s)	3201, 3236
CviQI	GTAC	9 cut(s)	394, 754, 1123, 1593, 1661, 2327, 2367, 2654, 3257
DraI	TTTAAA	1 cut(s)	1668
DraIII	CACNNNGTG	2 cut(s)	1216, 1979
DriI	GACNNNNNGTC	2 cut(s)	2233, 2313
EaeI	YGGCCR	2 cut(s)	69, 78
Eam1104I	CTCTTC	2 cut(s)	1182, 3299
Eam1105I	GACNNNNNGTC	2 cut(s)	2233, 2313
EarI	CTCTTC	2 cut(s)	1182, 3299
Eco130I	CCWWGG	6 cut(s)	14, 335, 357, 1923, 1953, 3271
Eco147I	AGGCCT	1 cut(s)	2024
Eco31I	GGTCTC	3 cut(s)	283, 550, 3387
Eco32I	GATATC	2 cut(s)	856, 2998
Eco47I	GGWCC	4 cut(s)	91, 888, 1423, 3490
Eco47III	AGCGCT	1 cut(s)	3342
Eco57I	CTGAAG	4 cut(s)	710, 1851, 2994, 3701
Eco81I	CCTNAGG	1 cut(s)	2553
Eco88I	CYCGRG	2 cut(s)	248, 402
EcoO109I	RGGNCCY	1 cut(s)	1423
EcoRI	GAATTC	2 cut(s)	1605, 3461
EcoRII	CCWGG	1 cut(s)	1783
EcoRV	GATATC	2 cut(s)	856, 2998
EcoT14I	CCWWGG	6 cut(s)	14, 335, 357, 1923, 1953, 3271
EcoT22I	ATGCAT	1 cut(s)	3063
ErhI	CCWWGG	6 cut(s)	14, 335, 357, 1923, 1953, 3271
Esp3I	CGTCTC	2 cut(s)	1892, 2241
FalI	AAGNNNNNCTT	4 cut(s)	32, 64, 968, 1000
FaqI	GGGAC	6 cut(s)	437, 901, 1409, 1761, 2983, 3063
FauI	CCCGC	1 cut(s)	3165
FauNDI	CATATG	2 cut(s)	1299, 1726
FbaI	TGATCA	3 cut(s)	102, 2389, 3241
FblI	GTMKAC	3 cut(s)	1354, 1792, 3030
FspBI	CTAG	5 cut(s)	474, 927, 2534, 2573, 3215
GlaI	GCGC	3 cut(s)	710, 2538, 3342
GsaI	CCCAGC	2 cut(s)	1205, 2213
GsuI	CTGGAG	4 cut(s)	618, 1506, 2229, 2478
HaeII	RGCGCY	1 cut(s)	3344
HaeIII	GGCC	7 cut(s)	71, 80, 1936, 1958, 2024, 2939, 3211
HapII	CCGG	5 cut(s)	89, 718, 891, 2606, 3236
HgaI	GACGC	2 cut(s)	62, 733
HhaI	GCGC	3 cut(s)	711, 2539, 3343
Hin1I	GRCGYC	1 cut(s)	54
Hin6I	GCGC	3 cut(s)	709, 2537, 3341
HinP1I	GCGC	3 cut(s)	709, 2537, 3341
HincII	GTYRAC	3 cut(s)	1355, 2421, 2603
HindII	GTYRAC	3 cut(s)	1355, 2421, 2603
HindIII	AAGCTT	1 cut(s)	1281
HpaI	GTTAAC	1 cut(s)	2421
HpaII	CCGG	5 cut(s)	89, 718, 891, 2606, 3236
HphI	GGTGA	6 cut(s)	545, 1403, 1427, 2162, 2163, 3196
Hpy99I	CGWCG	2 cut(s)	70, 619
HpyCH4III	ACNGT	7 cut(s)	158, 1261, 1533, 2014, 2315, 3028, 3312
HpyCH4IV	ACGT	2 cut(s)	2234, 2779
HpySE526I	ACGT	2 cut(s)	2234, 2779
Hsp92I	GRCGYC	1 cut(s)	54
HspAI	GCGC	3 cut(s)	709, 2537, 3341
Ksp22I	TGATCA	3 cut(s)	102, 2389, 3241
KspAI	GTTAAC	1 cut(s)	2421
LmnI	GCTCC	1 cut(s)	4
LweI	GCATC	6 cut(s)	22, 574, 943, 1960, 2725, 2908
MaeI	CTAG	5 cut(s)	474, 927, 2534, 2573, 3215
MaeII	ACGT	2 cut(s)	2234, 2779
MbiI	CCGCTC	1 cut(s)	3160
MfeI	CAATTG	4 cut(s)	906, 1464, 2175, 2694
MflI	RGATCY	2 cut(s)	406, 476
MhlI	GDGCHC	3 cut(s)	65, 829, 2869
MlsI	TGGCCA	1 cut(s)	80
MluNI	TGGCCA	1 cut(s)	80
MlyI	GAGTC	5 cut(s)	1488, 2211, 2361, 2612, 3631
MmeI	TCCRAC	3 cut(s)	137, 421, 3520
Mox20I	TGGCCA	1 cut(s)	80
Mph1103I	ATGCAT	1 cut(s)	3063
MroXI	GAANNNNTTC	1 cut(s)	2508
MscI	TGGCCA	1 cut(s)	80
MseI	TTAA	8 cut(s)	1053, 1442, 1667, 2301, 2420, 2589, 2649, 2982
MslI	CAYNNNNRTG	2 cut(s)	583, 1557
Msp20I	TGGCCA	1 cut(s)	80
MspA1I	CMGCKG	2 cut(s)	1238, 1940
MspI	CCGG	5 cut(s)	89, 718, 891, 2606, 3236
MspR9I	CCNGG	2 cut(s)	1785, 2607
MunI	CAATTG	4 cut(s)	906, 1464, 2175, 2694
Mva1269I	GAATGC	2 cut(s)	128, 574
MvaI	CCWGG	1 cut(s)	1785
MvnI	CGCG	1 cut(s)	2539
NciI	CCSGG	1 cut(s)	2607
NcoI	CCATGG	2 cut(s)	14, 1953
NdeI	CATATG	2 cut(s)	1299, 1726
NlaIV	GGNNCC	4 cut(s)	716, 889, 1425, 1520
NmeAIII	GCCGAG	1 cut(s)	152
NmuCI	GTSAC	4 cut(s)	152, 1480, 1979, 2896
NsiI	ATGCAT	1 cut(s)	3063
NspI	RCATGY	1 cut(s)	1107
NspV	TTCGAA	1 cut(s)	3472
PaeR7I	CTCGAG	2 cut(s)	248, 402
PagI	TCATGA	1 cut(s)	412
PceI	AGGCCT	1 cut(s)	2024
PciI	ACATGT	1 cut(s)	1103
PctI	GAATGC	2 cut(s)	128, 574
PdmI	GAANNNNTTC	1 cut(s)	2508
PfeI	GAWTC	8 cut(s)	634, 759, 1511, 1598, 3187, 3230, 3556, 3700
PinAI	ACCGGT	1 cut(s)	717
PleI	GAGTC	5 cut(s)	1487, 2210, 2360, 2612, 3631
PpsI	GAGTC	5 cut(s)	1487, 2210, 2360, 2612, 3631
PpuMI	RGGWCCY	1 cut(s)	1423
PscI	ACATGT	1 cut(s)	1103
PshAI	GACNNNNGTC	2 cut(s)	525, 2946
PsiI	TTATAA	2 cut(s)	1761, 3287
Psp1406I	AACGTT	1 cut(s)	2779
Psp5II	RGGWCCY	1 cut(s)	1423
Psp6I	CCWGG	1 cut(s)	1783
PspFI	CCCAGC	2 cut(s)	1201, 2209
PspGI	CCWGG	1 cut(s)	1783
PspN4I	GGNNCC	4 cut(s)	716, 889, 1425, 1520
PspPI	GGNCC	5 cut(s)	91, 888, 1423, 3209, 3490
PspPPI	RGGWCCY	1 cut(s)	1423
PspXI	VCTCGAGB	1 cut(s)	248
PstI	CTGCAG	3 cut(s)	963, 2874, 3090
PsuI	RGATCY	2 cut(s)	406, 476
PvuII	CAGCTG	2 cut(s)	1238, 1940
RsaI	GTAC	9 cut(s)	395, 755, 1124, 1594, 1662, 2328, 2368, 2655, 3258
RsaNI	GTAC	9 cut(s)	394, 754, 1123, 1593, 1661, 2327, 2367, 2654, 3257
RseI	CAYNNNNRTG	2 cut(s)	583, 1557
SalI	GTCGAC	1 cut(s)	1353
SaqAI	TTAA	8 cut(s)	1053, 1442, 1667, 2301, 2420, 2589, 2649, 2982
Sau96I	GGNCC	5 cut(s)	91, 888, 1423, 3209, 3490
SchI	GAGTC	5 cut(s)	1488, 2211, 2361, 2612, 3631
ScrFI	CCNGG	2 cut(s)	1785, 2607
SduI	GDGCHC	3 cut(s)	65, 829, 2869
SfaNI	GCATC	6 cut(s)	22, 574, 943, 1960, 2725, 2908
SfcI	CTRYAG	4 cut(s)	959, 2870, 3024, 3086
SfiI	GGCCNNNNNGGCC	1 cut(s)	77
Sfr274I	CTCGAG	2 cut(s)	248, 402
SfuI	TTCGAA	1 cut(s)	3472
SinI	GGWCC	4 cut(s)	91, 888, 1423, 3490
SlaI	CTCGAG	2 cut(s)	248, 402
SmiMI	CAYNNNNRTG	2 cut(s)	583, 1557
SmlI	CTYRAG	4 cut(s)	248, 402, 1406, 2346
SmoI	CTYRAG	4 cut(s)	248, 402, 1406, 2346
SseBI	AGGCCT	1 cut(s)	2024
SsiI	CCGC	8 cut(s)	147, 286, 851, 1045, 1930, 2539, 3134, 3158
SspI	AATATT	2 cut(s)	2815, 3106
SspMI	CTAG	5 cut(s)	474, 927, 2534, 2573, 3215
StuI	AGGCCT	1 cut(s)	2024
StyD4I	CCNGG	2 cut(s)	1783, 2605
StyI	CCWWGG	6 cut(s)	14, 335, 357, 1923, 1953, 3271
TaaI	ACNGT	7 cut(s)	158, 1261, 1533, 2014, 2315, 3028, 3312
TaiI	ACGT	2 cut(s)	2237, 2782
TaqI	TCGA	6 cut(s)	249, 403, 1354, 2317, 2442, 3472
TaqII	GACCGA	1 cut(s)	674
TatI	WGTACW	4 cut(s)	393, 1592, 2326, 2366
TauI	GCSGC	1 cut(s)	3137
TfiI	GAWTC	8 cut(s)	634, 759, 1511, 1598, 3187, 3230, 3556, 3700
Tru1I	TTAA	8 cut(s)	1053, 1442, 1667, 2301, 2420, 2589, 2649, 2982
Tru9I	TTAA	8 cut(s)	1053, 1442, 1667, 2301, 2420, 2589, 2649, 2982
TscAI	CASTG	9 cut(s)	1103, 1489, 1564, 2336, 2628, 2874, 2905, 3305, 3672
TseFI	GTSAC	4 cut(s)	152, 1480, 1979, 2896
Tsp45I	GTSAC	4 cut(s)	152, 1480, 1979, 2896
TspGWI	ACGGA	1 cut(s)	1436
TspRI	CASTG	9 cut(s)	1103, 1489, 1564, 2336, 2628, 2874, 2905, 3305, 3672
VpaK11BI	GGWCC	4 cut(s)	91, 888, 1423, 3490
XapI	RAATTY	5 cut(s)	467, 1055, 1605, 2218, 3461
XbaI	TCTAGA	1 cut(s)	473
XceI	RCATGY	1 cut(s)	1107
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3127
XhoI	CTCGAG	2 cut(s)	248, 402
XmiI	GTMKAC	3 cut(s)	1354, 1792, 3030
XmnI	GAANNNNTTC	1 cut(s)	2508
XspI	CTAG	5 cut(s)	474, 927, 2534, 2573, 3215
Zsp2I	ATGCAT	1 cut(s)	3063

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.