Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0004751.1

Orthogroup ID

OG0002865

Physical Location & Seq

Genomic Coordinates Reverse (-)

14388 .. 46598

Gene Structure 32211 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5802 bp

atgctgaccaggcaaactttagaagaacaagaattgatcgaagtacccaaccttgttgtggtacttcttctaattagtccaaaaagacgtttgcctcgaggcaaggaaagattggttaggcttgaagtcccgcagggacctaaatctcaattttacttggaggtaactttctccacactcccaaggggtgttacttcacaatccaaggaaatttacttctcaaagcataagaaatatgtatacttttctccagatcctgatggcaaggaacttttctcgcaatctgttggaaagatcttccctcgcgttgacaaatttcttccctcgggcaagatggtagctactcgctattacttccgcttccatttggtcgctgtcgcaccggaatttacgggtctcttctccggcgtctcctcaacagttcgcagattggaccattactacgatcttagttctccagtggtcaaccactcaacccgttggcaacaagtgaacagcacttgcgtggggcagccgtactgccacgtggtgcggctgatcaatcaacagcatcagttgcatccatatcggtgttcttggtctggagaggagactacaacaccggctcacagaaggatgaatcactcaattgggttgccttataacccaagcaatgacttggagaccggtcggaaggaggatcatgttgagcgcatgaaccggtggacgcaagcgaaactatatctgaaagcattgcaccgattccgtcacacactagtcctgaaaaaggcagaagaaaagaagcaggcagcaaggaaactacttagagcacatacccaagccctacgagctgcggctatcttcaaagaagttgcagatcaagtgaaagggtctggagattattttccaattgaaccagatgaacttgctcgtttgacgaggagtagtgatgttgatgctctgcagcaatatggggggaaggaaggcttggcagatttactgaaaacagatttagacaagggaattgatggggatgatttactaaagcggacaattaaatttggatcaaacatataccctccaaaaacagcaatgagccactggacatgtcttcgggaagctctgtacaattttactcggatcgttttggtgattgcttttatgggttctgttgtgatggtcttatctgagtatattcaagctcgtcagttccagcatatgattatcagggaggaaatcagaaacataaaattgaaggttattagagctggtcgacaagttgtggtttctaattgtgatattgttgttggtgatgttctacctcttaagactggtgatgaggtccctgctgatggcattttaatccgtggtcactcacttgcaattgataagagtagagtcactggagagagcaagatttttcacaaggattcaaggaacccatttctacgatctggctggaaagtagcacatggcagtgctactatgctggtaactggggttgggaaatgtactgaatcaggaattctcagggctaacacttattcaaaagacatcgaaaataaaacccccttgcaggtgcttttaacggaggttgctactttcatgggtgctgtcggattcatgggtgctgccattgttgcatatgttgtttggttcagagacttctttactggtgataattcaaatggaagtcctgaacctggaagtacacttgaagaagtgattagaattagtaccattgcagttgccatttttgtcgttgcattacctgaagtgcttccttcagctttcaacataagccgtctcagcacaatgaaaaagatgaaggcagacaatgccttggtgcggaggccagctgtatgtgaaaccatggccttatgcaatgatgcactttgtgacaagactggaataactttgaaccagatgactgtttctgaggcctatgctggtgagaagaaaatcaattttgctgacagcacaagcaacttgtctccgatactatcgtccttacttgttgaaggaattgctcagaatacgagtggcagtctatctgtgcttgggagtggtgatgttcaggcttctggatcaccaattgatagggcaattctgcattggggagtcaagctgggaatgaattttgatgatataatgtctcagtctccagttttgcaagttttgccattcagttcagagaaaaaacgaggtggtgttgcacttaatctgacacatggtcgagttcatgtacactggaaaggggcagctgaaggagtcttggcctcatgtacaagatacattggggctaatgatcaggttttaccgatggatgatgagaagttaacattttttcaaaaagctgtcgaggatatggcttctggaggtttgcattgcgttgctattgcatatagatcacttgaattggataatgttctaatcaatagtaatggacaactagcacagtgggaactacctgaggatgacctcattttactagctattgttggtatgaaggatgatagtcaaccggggatcaaggactcggtggaaatattgcaaagagctggcattaaggtacgccttgttacgcatgacaatcctcaaactgcaaaagcaattgctaaggaatgtagcatacttgcttctgatgggtcagatgttagtgaggaaaatcttattgaaggaaaagtgttccgagaactttctgatagtcagagagaaggattagcagagaagattttggtaatggcacggtcttcccccaatgacaagcttttgcttgtgcgagcactgcagaaaagaggaaagattgttgctgtcactggatatgccagtaatgatgctcatgcactacatgaggccgacattggtcttgctatgggtattcaaaggactgaagttgttaaagagagttcagatatcataatcttggatgacaattttgctacagtagccaagtgttttagatggggacgatcgatgcatgaaaaggttctgaaagttatgcagcagcaacttacagccaatattgtagcagtaaccataaactgcatggcggcagtttactatcgttacatcccgctcaatgctgtgcagtttgtgtgggtgaatcttatcatatttggtcttggaccactagcactggctactgaatctccggctgatcacctgatgaacgtaccacctattgaccgaggacaagctcttataaaatatattcagtggaagagactgttgacacaggttctgtaccaaattattgccctgcttgtcatcaattttcaaggagggagtttactgcaaaattataccagagaccatgccacaaaagtgaagaatacaatgatattcaacacatttgttgtttgtcagatttccattgaattcggagttcgaaagactggtatagtggaccatcctttgaaatggattactgctaactctgcgtttctgggaatagttggaataattcttttgattcagtttatcatcattgagtttcttggaaaggcgtttttcatagtcaggcttgactggaaagagtggcagttttcgctgactcttggcattgtcgagttgttgtttgcactgcttctatttctgaagcacctggcatatggaggggaaaacaaaaaacaaaaaaaaggaactgtcgagtatgggagaacaaaaactgaaaattccagtgataatattgatattcatgacttcttctggactcagcaatatgagacctatgacgagtttcccgtcgcacctgtgtctgtagctgtgagagtccgagatagcaggccattatccgctgtggcggcggcgaagagtaattccaatcctctgccgacgacgaggaaccctaacaaaaggagtaaagcagcagaagaagaagaagccgttgaagaagtggaagaggatctaccctggattcaagagaaggccttagacctggtggatttcaccggctctgtcacgcaggccattcctggacccagagttggcaccagctccctccattggattttggctattcctctcgcttatgccggtctcaattttgtcatcgctgttgtcaagaccgtgaaaaagttctcttcccctcgacataaacgcaagaaactgttgctgactgcttgtttgcaacttgcaggcccagttgtaatggatatggcaggttatactgaacggggttttaaaagaaaattagccgttcaagccttacatggaaaggtgttctatctgtcagagaacacgttatcagcacgagctctaaccctaaccctaatactcttctcacttaatttgccacaagccctagccccgcatccccgtgcttgccgcccccgcagccctacgctggcccactacctctgcaaccaacgcacacagctagaacatgctagtgtctgccccctacaatcctcagccttacagcctgccccctcgcatgccagggagtgcgcccctgcgccaaccagcctagcacacgccgtgcgatcgatgccacccgtagctctgcagcacctgcagcatcccctgcagggccctcacccgcgagcaccggcgagcgctcgcctgctctcacgttcgctctactgcgaccacgcgaccccgctacttccccgcgacactgcgacccagcgacctcaaccccacgattccgcgacctcgagactccttgacccgcggcctagtgactcagcgattacgcgacctcgctactccacgattctgagacaccgcgattcagcgattccgcaactctgcaactccgcaaaccccgcggaaagtctgcttgcttgcactaggattaaagcttgtctccaatcctactacaagaaccaaatacgttctgcaatcctaagaggtatgttttactataggttgaggaacctgaacaaattggactttgctccattgggaacaactagctctggatatcacaggtgggttcgtgatgtcagccagcatctcaaggccgatggaatcctggatacgattctcgagccttgtcaggatgtgctaaatgttgagcaagctcaagctttggaagcacatagagcagccttagaggcaaataaggcgaaagccatcatcctaatgactcgtcatatggatgattcgctccagtacgagtgtatgaatgaagaagaccccagaaggttgtgggtctcactcgaagaaagatttggcaacgtccgtgactccctgcctcctgacctagaagtgagatggcatagcctccgcttatgtgatttcaagtcaaggatgccacagagacaaccagacggaggagacgacccagctaaccaagtttcactgccagagatcaccggagctgcgagttcaagccgggtcagattgtccggatctggttcgggtcagccagatatttttgattatgaaggaatcccccaaacgctctgttctgtcattttgaaaccctcgacccgacccggacctgtttctttaacccggccggaactcgctcctccagcgacgccagaccacggcactaacccagatcgcttcctctcctccgtccggtcctccctacggtggcctctagctctgattctcacccaccgcagtgttttgaagtggcgaaacttggtggcgatagacccgatcgaaaaagtgtgggcgatgctcctccgagctgcatcaagtgccgacgggcagcgttgctctcgccaaacggaaccaggccacgatgttgactctcttcttgctactcgactcgtcgatgaagggttcaaagtcgaccttgatggtgcttccatggtttcaggcaagctcgctgagaatcggttcacctttgcttcgcgttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005215 GO:0005388 GO:0005575 GO:0005622 GO:0005623 GO:0005886 GO:0005887 GO:0005911 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0008150 GO:0008324 GO:0009506 GO:0015075 GO:0015085 GO:0015318 GO:0015399 GO:0015405 GO:0015662 GO:0016020 GO:0016021 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019829 GO:0022804 GO:0022853 GO:0022857 GO:0022890 GO:0030001 GO:0030054 GO:0031224 GO:0031226 GO:0034220 GO:0042623 GO:0042625 GO:0042626 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043492 GO:0044424 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055044 GO:0055085 GO:0070588 GO:0070838 GO:0071944 GO:0072511 GO:0090662 GO:0098655 GO:0098660 GO:0098662 GO:0099131 GO:0099132

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CaATP_NAI Cation_ATPase_C Cation_ATPase_N E1-E2_ATPase Hydrolase

Protein Analysis

1933

Amino Acids

214.92

Weight (kDa)

9.04

Isoelectric Point (pI)

46.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Cation_ATPase_N	PF00690	317 - 379	2.6e-07	Cation transporter/ATPase, N-terminus
E1-E2_ATPase	PF00122	410 - 501	2.4e-15	P-type ATPase actuator domain
Cation_ATPase	PF13246	666 - 834	1.7e-26	P-type ATPase, cytoplasmic domain N
Hydrolase	PF00702	823 - 947	2.1e-06	haloacid dehalogenase-like hydrolase
Cation_ATPase_C	PF00689	1018 - 1188	1.5e-24	Cation transporting ATPase, C-terminus
ARMH5	PF22915	1371 - 1406	1.4e-07	Armadillo-like helical domain-containing protein 5

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0002865)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g06912
pyrus_communis	pycom14g06520
rosa_chinensis	RchiOBHm_Chr4g0398821 RchiOBHm_Chr4g0398831 RchiOBHm_Chr4g0398851 RchiOBHm_Chr4g0398861
rosa_laevigata	RLG00000009311 RLG00000009312 RLG00000009316
rosa_multiflora	Rmu_sc0002885.1_g000007 Rmu_sc0002885.1_g000009 Rmu_sc0004474.1_g000001 Rmu_sc0004751.1_g000005
rosa_roxburghii	Rroxscaffold_173G00435010 Rroxscaffold_5G00343860 Rroxscaffold_5G00343900 Rroxscaffold_5G00355000
rosa_rugosa	Rorug04G0012100 Rorug04G0012300 Rorug04G0012400 Rorug04G0012600
rosa_samantha	Rh4AG090100 Rh4AG090400 Rh4BG087200 Rh4BG087500 Rh4BG087600 Rh4BG087700 Rh4CG098200 Rh4CG098400 Rh4CG098700 Rh4DG081500 Rh4DG081600 Rh4DG081800 Rh4DG082000 Rh4DG082300 Rh4DG082700
rosa_wichuraiana	Rw4G007420 Rw4G007440 Rw4G007480

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	642, 3185
AarI	CACCTGC	2 cut(s)	1546, 4503
AbsI	CCTCGAGG	1 cut(s)	96
Acc36I	ACCTGC	3 cut(s)	1546, 4133, 4503
AccB1I	GGYRCC	1 cut(s)	3971
AccBSI	CCGCTC	1 cut(s)	3058
AccI	GTMKAC	3 cut(s)	240, 1252, 5733
AccIII	TCCGGA	1 cut(s)	5336
AclWI	GGATC	8 cut(s)	248, 687, 1051, 1127, 2074, 2516, 3894, 5347
AcoI	YGGCCR	1 cut(s)	5447
AcsI	RAATTY	8 cut(s)	210, 314, 386, 1037, 1503, 2116, 3359, 3655
AcuI	CTGAAG	5 cut(s)	1749, 1773, 2265, 2892, 3599
AcvI	CACGTG	1 cut(s)	526
AcyI	GRCGYC	2 cut(s)	408, 5471
AdeI	CACNNNGTG	3 cut(s)	529, 1877, 4462
AfeI	AGCGCT	1 cut(s)	4540
AflII	CTTAAG	1 cut(s)	1304
AflIII	ACRYGT	2 cut(s)	1085, 4221
AgeI	ACCGGT	2 cut(s)	665, 699
AhdI	GACNNNNNGTC	1 cut(s)	2211
AhlI	ACTAGT	1 cut(s)	754
AjnI	CCWGG	9 cut(s)	8, 1681, 3585, 3893, 3918, 3955, 4421, 4959, 5674
AjuI	GAANNNNNNNTTGG	4 cut(s)	950, 982, 5142, 5174
AleI	CACNNNNGTG	3 cut(s)	503, 3305, 5559
Alw21I	GWGCWC	4 cut(s)	811, 2767, 4240, 4531
AlwI	GGATC	8 cut(s)	248, 687, 1051, 1127, 2074, 2516, 3894, 5347
AlwNI	CAGNNNCTG	3 cut(s)	257, 3223, 4495
Ama87I	CYCGRG	4 cut(s)	96, 325, 4639, 4973
Aor13HI	TCCGGA	1 cut(s)	5336
Aor51HI	AGCGCT	1 cut(s)	4540
ApaI	GGGCCC	1 cut(s)	4517
ApoI	RAATTY	8 cut(s)	210, 314, 386, 1037, 1503, 2116, 3359, 3655
ArsI	GACNNNNNNTTYG	2 cut(s)	4090, 4122
AsiGI	ACCGGT	2 cut(s)	665, 699
Asp700I	GAANNNNTTC	4 cut(s)	1758, 2967, 4058, 5780
AspLEI	GCGC	4 cut(s)	693, 4433, 4441, 4541
AsuC2I	CCSGG	4 cut(s)	2505, 5324, 5427, 5446
AsuII	TTCGAA	1 cut(s)	3370
AvaI	CYCGRG	4 cut(s)	96, 325, 4639, 4973
AvaII	GGWCC	8 cut(s)	137, 433, 1321, 3107, 3388, 3959, 5429, 5517
AxyI	CCTNAGG	1 cut(s)	2451
BaeGI	GKGCMC	1 cut(s)	4517
BaeI	ACNNNNGTAYC	2 cut(s)	1970, 2003
BanI	GGYRCC	1 cut(s)	3971
BanII	GRGCYC	2 cut(s)	4240, 4517
BarI	GAAGNNNNNNTAC	2 cut(s)	3873, 3905
BauI	CACGAG	1 cut(s)	4233
BbrPI	CACGTG	1 cut(s)	526
BbsI	GAAGAC	3 cut(s)	1082, 2724, 5127
Bbv12I	GWGCWC	4 cut(s)	811, 2767, 4240, 4531
BbvCI	CCTCAGC	1 cut(s)	4393
BceAI	ACGGC	6 cut(s)	499, 1767, 3851, 4163, 4445, 5497
BcgI	CGANNNNNNTGC	2 cut(s)	548, 582
BciT130I	CCWGG	9 cut(s)	10, 1683, 3587, 3895, 3920, 3957, 4423, 4961, 5676
BciVI	GTATCC	1 cut(s)	4957
BclI	TGATCA	3 cut(s)	537, 2287, 3139
BcnI	CCSGG	4 cut(s)	2505, 5324, 5427, 5446
BcuI	ACTAGT	1 cut(s)	754
BfmI	CTRYAG	8 cut(s)	941, 2768, 2922, 3741, 4487, 4496, 4508, 4849
BfoI	RGCGCY	1 cut(s)	4542
BfrI	CTTAAG	1 cut(s)	1304
BfuAI	ACCTGC	3 cut(s)	1546, 4133, 4503
BfuI	GTATCC	1 cut(s)	4957
BglI	GCCNNNNNGGC	1 cut(s)	5042
BglII	AGATCT	1 cut(s)	294
Bme18I	GGWCC	8 cut(s)	137, 433, 1321, 3107, 3388, 3959, 5429, 5517
BmeRI	GACNNNNNGTC	1 cut(s)	2211
BmeT110I	CYCGRG	4 cut(s)	96, 325, 4639, 4973
BmiI	GGNNCC	9 cut(s)	138, 1323, 1418, 3827, 3961, 3973, 4515, 4862, 5673
BmrI	ACTGGG	2 cut(s)	1485, 4118
BmuI	ACTGGG	2 cut(s)	1485, 4118
BoxI	GACNNNNGTC	1 cut(s)	2844
BpiI	GAAGAC	3 cut(s)	1082, 2724, 5127
BpmI	CTGGAG	9 cut(s)	234, 441, 603, 894, 1404, 2127, 2376, 5081, 5448
Bpu10I	CCTNAGC	2 cut(s)	2598, 4393
Bpu14I	TTCGAA	1 cut(s)	3370
BpuEI	CTTGAG	2 cut(s)	4928, 4995
BpuMI	CCSGG	4 cut(s)	2505, 5324, 5427, 5446
Bsa29I	ATCGAT	2 cut(s)	2954, 4469
BsaAI	YACGTR	1 cut(s)	526
BsaHI	GRCGYC	2 cut(s)	408, 5471
BsaI	GGTCTC	6 cut(s)	401, 656, 3285, 3701, 4025, 5146
BsaWI	WCCGGW	6 cut(s)	382, 665, 699, 5303, 5336, 5514
BsaXI	ACNNNNNCTCC	2 cut(s)	3115, 3145
Bse118I	RCCGGY	6 cut(s)	601, 665, 699, 3932, 4016, 4531
Bse21I	CCTNAGG	1 cut(s)	2451
Bse3DI	GCAATG	6 cut(s)	658, 731, 1077, 1719, 1870, 2365
BseAI	TCCGGA	1 cut(s)	5336
BseBI	CCWGG	9 cut(s)	10, 1683, 3587, 3895, 3920, 3957, 4423, 4961, 5676
BseCI	ATCGAT	2 cut(s)	2954, 4469
BseMI	GCAATG	6 cut(s)	658, 731, 1077, 1719, 1870, 2365
BseRI	GAGGAG	7 cut(s)	403, 602, 934, 5277, 5451, 5497, 5612
BseSI	GKGCMC	1 cut(s)	4517
BseX3I	CGGCCG	1 cut(s)	5447
BseYI	CCCAGC	3 cut(s)	2107, 4608, 5272
BsgI	GTGCAG	1 cut(s)	3089
Bsh1285I	CGRYCG	5 cut(s)	670, 2954, 4469, 5450, 5601
BshNI	GGYRCC	1 cut(s)	3971
BshTI	ACCGGT	2 cut(s)	665, 699
BshVI	ATCGAT	2 cut(s)	2954, 4469
BsiEI	CGRYCG	5 cut(s)	670, 2954, 4469, 5450, 5601
BsiHKAI	GWGCWC	4 cut(s)	811, 2767, 4240, 4531
BsiHKCI	CYCGRG	4 cut(s)	96, 325, 4639, 4973
BslFI	GGGAC	4 cut(s)	113, 150, 1307, 2961
BsmBI	CGTCTC	3 cut(s)	415, 1790, 5259
BsmFI	GGGAC	4 cut(s)	113, 150, 1307, 2961
Bso31I	GGTCTC	6 cut(s)	401, 656, 3285, 3701, 4025, 5146
BsoBI	CYCGRG	4 cut(s)	96, 325, 4639, 4973
Bsp119I	TTCGAA	1 cut(s)	3370
Bsp120I	GGGCCC	1 cut(s)	4513
Bsp1286I	GDGCHC	5 cut(s)	811, 2767, 4240, 4517, 4531
Bsp13I	TCCGGA	1 cut(s)	5336
Bsp1407I	TGTACA	3 cut(s)	1104, 2224, 2264
Bsp19I	CCATGG	2 cut(s)	1851, 5751
BspDI	ATCGAT	2 cut(s)	2954, 4469
BspEI	TCCGGA	1 cut(s)	5336
BspHI	TCATGA	1 cut(s)	3679
BspLI	GGNNCC	9 cut(s)	138, 1323, 1418, 3827, 3961, 3973, 4515, 4862, 5673
BspMAI	CTGCAG	5 cut(s)	945, 2772, 4491, 4500, 4512
BspMI	ACCTGC	3 cut(s)	1546, 4133, 4503
BspPI	GGATC	8 cut(s)	248, 687, 1051, 1127, 2074, 2516, 3894, 5347
BspT104I	TTCGAA	1 cut(s)	3370
BspT107I	GGYRCC	1 cut(s)	3971
BspTI	CTTAAG	1 cut(s)	1304
BspTNI	GGTCTC	6 cut(s)	401, 656, 3285, 3701, 4025, 5146
BsrBI	CCGCTC	1 cut(s)	3058
BsrDI	GCAATG	6 cut(s)	658, 731, 1077, 1719, 1870, 2365
BsrFI	RCCGGY	6 cut(s)	601, 665, 699, 3932, 4016, 4531
BsrGI	TGTACA	3 cut(s)	1104, 2224, 2264
BssAI	RCCGGY	6 cut(s)	601, 665, 699, 3932, 4016, 4531
BssNAI	GTATAC	1 cut(s)	241
BssNI	GRCGYC	2 cut(s)	408, 5471
BssSI	CACGAG	1 cut(s)	4233
BssT1I	CCWWGG	5 cut(s)	182, 204, 1821, 1851, 5751
Bst1107I	GTATAC	1 cut(s)	241
Bst2BI	CACGAG	1 cut(s)	4233
Bst2UI	CCWGG	9 cut(s)	10, 1683, 3587, 3895, 3920, 3957, 4423, 4961, 5676
Bst6I	CTCTTC	7 cut(s)	404, 3197, 3788, 3876, 4069, 4265, 5700
BstACI	GRCGYC	2 cut(s)	408, 5471
BstAFI	CTTAAG	1 cut(s)	1304
BstAPI	GCANNNNNTGC	6 cut(s)	556, 1817, 2158, 4495, 4507, 5639
BstAUI	TGTACA	3 cut(s)	1104, 2224, 2264
BstBAI	YACGTR	1 cut(s)	526
BstBI	TTCGAA	1 cut(s)	3370
BstDSI	CCRYGG	6 cut(s)	1345, 1851, 4655, 4752, 5479, 5751
BstENI	CCTNNNNNAGG	1 cut(s)	764
BstH2I	RGCGCY	1 cut(s)	4542
BstHHI	GCGC	4 cut(s)	693, 4433, 4441, 4541
BstMCI	CGRYCG	5 cut(s)	670, 2954, 4469, 5450, 5601
BstNI	CCWGG	9 cut(s)	10, 1683, 3587, 3895, 3920, 3957, 4423, 4961, 5676
BstNSI	RCATGY	3 cut(s)	1089, 4370, 4421
BstPAI	GACNNNNGTC	1 cut(s)	2844
BstSFI	CTRYAG	8 cut(s)	941, 2768, 2922, 3741, 4487, 4496, 4508, 4849
BstSLI	GKGCMC	1 cut(s)	4517
BstV2I	GAAGAC	3 cut(s)	1082, 2724, 5127
BstX2I	RGATCY	4 cut(s)	253, 294, 3886, 5339
BstYI	RGATCY	4 cut(s)	253, 294, 3886, 5339
BstZ17I	GTATAC	1 cut(s)	241
BstZI	CGGCCG	1 cut(s)	5447
Bsu15I	ATCGAT	2 cut(s)	2954, 4469
Bsu36I	CCTNAGG	1 cut(s)	2451
BsuI	GTATCC	1 cut(s)	4957
BsuTUI	ATCGAT	2 cut(s)	2954, 4469
BtgI	CCRYGG	6 cut(s)	1345, 1851, 4655, 4752, 5479, 5751
BtgZI	GCGATG	2 cut(s)	4018, 5630
BtsI	GCAGTG	6 cut(s)	1462, 2765, 3563, 4599, 5288, 5566
BveI	ACCTGC	3 cut(s)	1546, 4133, 4503
CaiI	CAGNNNCTG	3 cut(s)	257, 3223, 4495
CciI	TCATGA	1 cut(s)	3679
CfoI	GCGC	4 cut(s)	693, 4433, 4441, 4541
Cfr10I	RCCGGY	6 cut(s)	601, 665, 699, 3932, 4016, 4531
Cfr42I	CCGCGG	2 cut(s)	4658, 4755
ClaI	ATCGAT	2 cut(s)	2954, 4469
CseI	GACGC	3 cut(s)	397, 715, 5479
CsiI	ACCWGGT	1 cut(s)	3918
CspAI	ACCGGT	2 cut(s)	665, 699
DraI	TTTAAA	1 cut(s)	4165
DraIII	CACNNNGTG	3 cut(s)	529, 1877, 4462
DriI	GACNNNNNGTC	1 cut(s)	2211
EaeI	YGGCCR	1 cut(s)	5447
EagI	CGGCCG	1 cut(s)	5447
Eam1104I	CTCTTC	7 cut(s)	404, 3197, 3788, 3876, 4069, 4265, 5700
Eam1105I	GACNNNNNGTC	1 cut(s)	2211
EarI	CTCTTC	7 cut(s)	404, 3197, 3788, 3876, 4069, 4265, 5700
Ecl136II	GAGCTC	1 cut(s)	4238
EclXI	CGGCCG	1 cut(s)	5447
Eco130I	CCWWGG	5 cut(s)	182, 204, 1821, 1851, 5751
Eco147I	AGGCCT	2 cut(s)	1922, 3911
Eco24I	GRGCYC	2 cut(s)	4240, 4517
Eco31I	GGTCTC	6 cut(s)	401, 656, 3285, 3701, 4025, 5146
Eco32I	GATATC	2 cut(s)	2896, 4910
Eco47I	GGWCC	8 cut(s)	137, 433, 1321, 3107, 3388, 3959, 5429, 5517
Eco47III	AGCGCT	1 cut(s)	4540
Eco52I	CGGCCG	1 cut(s)	5447
Eco53kI	GAGCTC	1 cut(s)	4238
Eco57I	CTGAAG	5 cut(s)	1749, 1773, 2265, 2892, 3599
Eco72I	CACGTG	1 cut(s)	526
Eco81I	CCTNAGG	1 cut(s)	2451
Eco88I	CYCGRG	4 cut(s)	96, 325, 4639, 4973
EcoICRI	GAGCTC	1 cut(s)	4238
EcoNI	CCTNNNNNAGG	1 cut(s)	764
EcoO109I	RGGNCCY	4 cut(s)	137, 1321, 4513, 4514
EcoRI	GAATTC	2 cut(s)	1503, 3359
EcoRII	CCWGG	9 cut(s)	8, 1681, 3585, 3893, 3918, 3955, 4421, 4959, 5674
EcoRV	GATATC	2 cut(s)	2896, 4910
EcoT14I	CCWWGG	5 cut(s)	182, 204, 1821, 1851, 5751
EcoT22I	ATGCAT	1 cut(s)	2961
EcoT38I	GRGCYC	2 cut(s)	4240, 4517
ErhI	CCWWGG	5 cut(s)	182, 204, 1821, 1851, 5751
Esp3I	CGTCTC	3 cut(s)	415, 1790, 5259
FalI	AAGNNNNNCTT	4 cut(s)	950, 982, 5721, 5753
FaqI	GGGAC	4 cut(s)	113, 150, 1307, 2961
FauI	CCCGC	9 cut(s)	138, 3063, 4299, 4322, 4530, 4590, 4601, 4662, 4759
FauNDI	CATATG	4 cut(s)	1197, 1624, 3592, 5082
FbaI	TGATCA	3 cut(s)	537, 2287, 3139
FblI	GTMKAC	3 cut(s)	240, 1252, 5733
FriOI	GRGCYC	2 cut(s)	4240, 4517
GlaI	GCGC	4 cut(s)	692, 4432, 4440, 4540
GsaI	CCCAGC	3 cut(s)	2111, 4612, 5276
GsuI	CTGGAG	9 cut(s)	234, 441, 603, 894, 1404, 2127, 2376, 5081, 5448
HaeII	RGCGCY	1 cut(s)	4542
HgaI	GACGC	3 cut(s)	397, 715, 5479
HhaI	GCGC	4 cut(s)	693, 4433, 4441, 4541
Hin1I	GRCGYC	2 cut(s)	408, 5471
Hin6I	GCGC	4 cut(s)	691, 4431, 4439, 4539
HinP1I	GCGC	4 cut(s)	691, 4431, 4439, 4539
HincII	GTYRAC	8 cut(s)	310, 466, 1253, 2319, 2501, 3213, 5689, 5734
HindII	GTYRAC	8 cut(s)	310, 466, 1253, 2319, 2501, 3213, 5689, 5734
HindIII	AAGCTT	3 cut(s)	2747, 4785, 5013
HpaI	GTTAAC	1 cut(s)	2319
Hpy99I	CGWCG	6 cut(s)	3731, 3820, 3823, 5473, 5648, 5717
HpyCH4IV	ACGT	7 cut(s)	88, 525, 3153, 4223, 4555, 4819, 5166
HpySE526I	ACGT	7 cut(s)	88, 525, 3153, 4223, 4555, 4819, 5166
Hsp92I	GRCGYC	2 cut(s)	408, 5471
HspAI	GCGC	4 cut(s)	691, 4431, 4439, 4539
Kpn2I	TCCGGA	1 cut(s)	5336
Ksp22I	TGATCA	3 cut(s)	537, 2287, 3139
KspAI	GTTAAC	1 cut(s)	2319
KspI	CCGCGG	2 cut(s)	4658, 4755
LmnI	GCTCC	6 cut(s)	3983, 4888, 5100, 5306, 5464, 5625
MabI	ACCWGGT	1 cut(s)	3918
MaeII	ACGT	7 cut(s)	88, 525, 3153, 4223, 4555, 4819, 5166
MbiI	CCGCTC	1 cut(s)	3058
MfeI	CAATTG	5 cut(s)	627, 888, 1362, 2073, 2592
MflI	RGATCY	4 cut(s)	253, 294, 3886, 5339
MhlI	GDGCHC	5 cut(s)	811, 2767, 4240, 4517, 4531
MmeI	TCCRAC	4 cut(s)	268, 650, 1576, 3418
Mph1103I	ATGCAT	1 cut(s)	2961
MroI	TCCGGA	1 cut(s)	5336
MroXI	GAANNNNTTC	4 cut(s)	1758, 2967, 4058, 5780
MslI	CAYNNNNRTG	9 cut(s)	503, 568, 1455, 3305, 4299, 4371, 5069, 5085, 5559
MspA1I	CMGCKG	5 cut(s)	1838, 2243, 3779, 4657, 4754
MspCI	CTTAAG	1 cut(s)	1304
MunI	CAATTG	5 cut(s)	627, 888, 1362, 2073, 2592
MvaI	CCWGG	9 cut(s)	10, 1683, 3587, 3895, 3920, 3957, 4423, 4961, 5676
NciI	CCSGG	4 cut(s)	2505, 5324, 5427, 5446
NcoI	CCATGG	2 cut(s)	1851, 5751
NdeI	CATATG	4 cut(s)	1197, 1624, 3592, 5082
NlaIV	GGNNCC	9 cut(s)	138, 1323, 1418, 3827, 3961, 3973, 4515, 4862, 5673
NmuCI	GTSAC	8 cut(s)	746, 1349, 1378, 1877, 2794, 3940, 4664, 5171
NsiI	ATGCAT	1 cut(s)	2961
NspI	RCATGY	3 cut(s)	1089, 4370, 4421
NspV	TTCGAA	1 cut(s)	3370
OliI	CACNNNNGTG	3 cut(s)	503, 3305, 5559
PaeI	GCATGC	1 cut(s)	4421
PaeR7I	CTCGAG	3 cut(s)	96, 4639, 4973
PagI	TCATGA	1 cut(s)	3679
PaqCI	CACCTGC	2 cut(s)	1546, 4503
PceI	AGGCCT	2 cut(s)	1922, 3911
PciI	ACATGT	1 cut(s)	1085
PcsI	WCGNNNNNNNCGW	3 cut(s)	94, 3723, 5265
PdmI	GAANNNNTTC	4 cut(s)	1758, 2967, 4058, 5780
PfoI	TCCNGGA	2 cut(s)	3955, 4959
PinAI	ACCGGT	2 cut(s)	665, 699
Ple19I	CGATCG	3 cut(s)	2954, 4469, 5601
PmaCI	CACGTG	1 cut(s)	526
PmlI	CACGTG	1 cut(s)	526
Ppu21I	YACGTR	1 cut(s)	526
PpuMI	RGGWCCY	2 cut(s)	137, 1321
PscI	ACATGT	1 cut(s)	1085
PshAI	GACNNNNGTC	1 cut(s)	2844
PsiI	TTATAA	2 cut(s)	642, 3185
Psp124BI	GAGCTC	1 cut(s)	4240
Psp5II	RGGWCCY	2 cut(s)	137, 1321
Psp6I	CCWGG	9 cut(s)	8, 1681, 3585, 3893, 3918, 3955, 4421, 4959, 5674
PspCI	CACGTG	1 cut(s)	526
PspFI	CCCAGC	3 cut(s)	2107, 4608, 5272
PspGI	CCWGG	9 cut(s)	8, 1681, 3585, 3893, 3918, 3955, 4421, 4959, 5674
PspN4I	GGNNCC	9 cut(s)	138, 1323, 1418, 3827, 3961, 3973, 4515, 4862, 5673
PspOMI	GGGCCC	1 cut(s)	4513
PspPPI	RGGWCCY	2 cut(s)	137, 1321
PspXI	VCTCGAGB	1 cut(s)	96
PstI	CTGCAG	5 cut(s)	945, 2772, 4491, 4500, 4512
PstNI	CAGNNNCTG	3 cut(s)	257, 3223, 4495
PsuI	RGATCY	4 cut(s)	253, 294, 3886, 5339
PvuI	CGATCG	3 cut(s)	2954, 4469, 5601
PvuII	CAGCTG	2 cut(s)	1838, 2243
RseI	CAYNNNNRTG	9 cut(s)	503, 568, 1455, 3305, 4299, 4371, 5069, 5085, 5559
SacI	GAGCTC	1 cut(s)	4240
SacII	CCGCGG	2 cut(s)	4658, 4755
SalI	GTCGAC	2 cut(s)	1251, 5732
SbfI	CCTGCAGG	1 cut(s)	4512
SdaI	CCTGCAGG	1 cut(s)	4512
SduI	GDGCHC	5 cut(s)	811, 2767, 4240, 4517, 4531
SexAI	ACCWGGT	1 cut(s)	3918
SfcI	CTRYAG	8 cut(s)	941, 2768, 2922, 3741, 4487, 4496, 4508, 4849
Sfr274I	CTCGAG	3 cut(s)	96, 4639, 4973
Sfr303I	CCGCGG	2 cut(s)	4658, 4755
SfuI	TTCGAA	1 cut(s)	3370
SgrAI	CRCCGGYG	1 cut(s)	4531
SgrBI	CCGCGG	2 cut(s)	4658, 4755
SinI	GGWCC	8 cut(s)	137, 433, 1321, 3107, 3388, 3959, 5429, 5517
SlaI	CTCGAG	3 cut(s)	96, 4639, 4973
SmiMI	CAYNNNNRTG	9 cut(s)	503, 568, 1455, 3305, 4299, 4371, 5069, 5085, 5559
SmlI	CTYRAG	6 cut(s)	96, 1304, 4639, 4943, 4973, 5010
SmoI	CTYRAG	6 cut(s)	96, 1304, 4639, 4943, 4973, 5010
SpeI	ACTAGT	1 cut(s)	754
SphI	GCATGC	1 cut(s)	4421
Sse8387I	CCTGCAGG	1 cut(s)	4512
SseBI	AGGCCT	2 cut(s)	1922, 3911
SspI	AATATT	3 cut(s)	2529, 3004, 3670
SstI	GAGCTC	1 cut(s)	4240
StuI	AGGCCT	2 cut(s)	1922, 3911
StyI	CCWWGG	5 cut(s)	182, 204, 1821, 1851, 5751
TaiI	ACGT	7 cut(s)	91, 528, 3156, 4226, 4558, 4822, 5169
TaqII	GACCGA	1 cut(s)	3183
TatI	WGTACW	5 cut(s)	1104, 1490, 1688, 2224, 2264
TauI	GCSGC	7 cut(s)	535, 836, 3035, 3788, 3791, 4311, 4660
TseFI	GTSAC	8 cut(s)	746, 1349, 1378, 1877, 2794, 3940, 4664, 5171
Tsp45I	GTSAC	8 cut(s)	746, 1349, 1378, 1877, 2794, 3940, 4664, 5171
TspGWI	ACGGA	7 cut(s)	734, 1334, 1583, 5159, 5274, 5500, 5684
Vha464I	CTTAAG	1 cut(s)	1304
VpaK11BI	GGWCC	8 cut(s)	137, 433, 1321, 3107, 3388, 3959, 5429, 5517
XagI	CCTNNNNNAGG	1 cut(s)	764
XapI	RAATTY	8 cut(s)	210, 314, 386, 1037, 1503, 2116, 3359, 3655
XceI	RCATGY	3 cut(s)	1089, 4370, 4421
XcmI	CCANNNNNNNNNTGG	2 cut(s)	55, 3025
XhoI	CTCGAG	3 cut(s)	96, 4639, 4973
XmiI	GTMKAC	3 cut(s)	240, 1252, 5733
XmnI	GAANNNNTTC	4 cut(s)	1758, 2967, 4058, 5780
Zsp2I	ATGCAT	1 cut(s)	2961

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.