Rmu_sc0007019.1_g000006

UPF0481 protein At3g47200-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007019.1
OG0002269
Physical Location & Seq
Reverse (-)
35663 .. 36448
786 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 786 bp
atgggcaagtgtgtgcatgtgttggacatgtacaggaagagtctgcttcagcatgagcctaatcccaaatttattcgcaaaataaggccaagtgagcatgacgatgagatcatctggtccgcaatagcgctcaatgaagcaggaatcaggttcaagaaaagtgcaactacaagcctcaaagacatcacattcgcccggggagtgttgaggctccctgttgttgttgtggatgacaccaccgagtccatgttcttgaatctgatggcgtttgaaagattccatgtaggagcaggaaatgaggtgacatcttatttactcttcatggacaacatcatagacaatgctatggatgttgccctcttacactcgaaagggatcatccagaatgctttggggagtgacaaagcagtggctaatctcttcaattcactgtctaaggatgtgacacaggacccggatagcagccttcaaattgtgcataagagggtgtatcactactgcagaaagccgtggaatgagtggcgcgccaatcttattcacacttacttccgaaatccatgggctattatctctttcattgctggtgtcttcctctttgtgttcactatagctcagactggatatgccatatatccctattaccatccactaaatgattcttctccttccactgtcattctcgttccggctcctcctccaaaacattcatcctccgactccacctccccatcgacacttgtgatttctttcatccttgttaccatggtatgcatgctgaccaattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF247

Protein Analysis

261

Amino Acids

29.43

Weight (kDa)

7.78

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF247 PF03140 3 - 200 2.7e-65 Plant protein of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 525
AciI CCGC 1 cut(s) 120
AclWI GGATC 1 cut(s) 383
AcsI RAATTY 1 cut(s) 68
AcuI CTGAAG 1 cut(s) 32
AfaI GTAC 1 cut(s) 32
AfeI AGCGCT 1 cut(s) 129
AflIII ACRYGT 1 cut(s) 27
AgsI TTSAA 5 cut(s) 154, 256, 272, 424, 470
AluBI AGCT 1 cut(s) 611
AluI AGCT 1 cut(s) 611
AlwI GGATC 1 cut(s) 383
Ama87I CYCGRG 1 cut(s) 195
Aor51HI AGCGCT 1 cut(s) 129
AoxI GGCC 1 cut(s) 86
ApeKI GCWGC 1 cut(s) 462
ApoI RAATTY 1 cut(s) 68
ArsI GACNNNNNNTTYG 2 cut(s) 173, 205
AscI GGCGCGCC 1 cut(s) 523
AspLEI GCGC 3 cut(s) 130, 525, 527
AspS9I GGNCC 2 cut(s) 117, 451
AsuC2I CCSGG 3 cut(s) 196, 197, 455
AsuHPI GGTGA 1 cut(s) 313
AvaI CYCGRG 1 cut(s) 195
AvaII GGWCC 2 cut(s) 117, 451
BbsI GAAGAC 1 cut(s) 580
BbvI GCAGC 1 cut(s) 474
BccI CCATC 3 cut(s) 256, 651, 736
BceAI ACGGC 1 cut(s) 493
BcnI CCSGG 3 cut(s) 196, 197, 455
BfmI CTRYAG 2 cut(s) 499, 606
BfoI RGCGCY 1 cut(s) 131
BisI GCNGC 1 cut(s) 463
BlsI GCNGC 1 cut(s) 464
Bme1390I CCNGG 3 cut(s) 196, 197, 455
Bme18I GGWCC 2 cut(s) 117, 451
BmeT110I CYCGRG 1 cut(s) 195
BmgT120I GGNCC 2 cut(s) 117, 451
BmiI GGNNCC 3 cut(s) 212, 453, 690
BmrFI CCNGG 3 cut(s) 196, 197, 455
BpiI GAAGAC 1 cut(s) 580
BpuMI CCSGG 3 cut(s) 196, 197, 455
BsaJI CCNNGG 5 cut(s) 195, 196, 509, 557, 762
BsaXI ACNNNNNCTCC 2 cut(s) 707, 737
Bse1I ACTGG 1 cut(s) 622
Bse3DI GCAATG 1 cut(s) 576
BseDI CCNNGG 5 cut(s) 195, 196, 509, 557, 762
BseGI GGATG 7 cut(s) 235, 355, 378, 445, 643, 707, 750
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 1 cut(s) 626
BseNI ACTGG 1 cut(s) 622
BsePI GCGCGC 1 cut(s) 523
BseRI GAGGAG 2 cut(s) 681, 684
BseXI GCAGC 1 cut(s) 474
Bsh1236I CGCG 1 cut(s) 525
BshFI GGCC 1 cut(s) 88
BsiHKCI CYCGRG 1 cut(s) 195
BsiSI CCGG 3 cut(s) 196, 455, 686
BsmI GAATGC 1 cut(s) 391
BsnI GGCC 1 cut(s) 88
BsoBI CYCGRG 1 cut(s) 195
Bsp1407I TGTACA 1 cut(s) 30
Bsp143I GATC 2 cut(s) 108, 375
Bsp19I CCATGG 2 cut(s) 557, 762
BspACI CCGC 1 cut(s) 120
BspANI GGCC 1 cut(s) 88
BspCNI CTCAG 1 cut(s) 625
BspFNI CGCG 1 cut(s) 525
BspLI GGNNCC 3 cut(s) 212, 453, 690
BspMAI CTGCAG 1 cut(s) 503
BspPI GGATC 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 576
BsrGI TGTACA 1 cut(s) 30
BsrI ACTGG 1 cut(s) 622
BssECI CCNNGG 5 cut(s) 195, 196, 509, 557, 762
BssHII GCGCGC 1 cut(s) 523
BssMI GATC 2 cut(s) 108, 375
BssT1I CCWWGG 2 cut(s) 557, 762
Bst4CI ACNGT 2 cut(s) 432, 673
Bst6I CTCTTC 3 cut(s) 32, 323, 425
BstAUI TGTACA 1 cut(s) 30
BstC8I GCNNGC 2 cut(s) 525, 773
BstDEI CTNAG 2 cut(s) 435, 612
BstDSI CCRYGG 3 cut(s) 509, 557, 762
BstF5I GGATG 7 cut(s) 235, 355, 378, 445, 643, 707, 750
BstFNI CGCG 1 cut(s) 525
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 3 cut(s) 130, 525, 527
BstKTI GATC 2 cut(s) 111, 378
BstMBI GATC 2 cut(s) 108, 375
BstMWI GCNNNNNNNGC 1 cut(s) 94
BstNSI RCATGY 3 cut(s) 20, 31, 775
BstSCI CCNGG 3 cut(s) 194, 195, 453
BstSFI CTRYAG 2 cut(s) 499, 606
BstUI CGCG 1 cut(s) 525
BstV1I GCAGC 1 cut(s) 474
BstV2I GAAGAC 1 cut(s) 580
BsuRI GGCC 1 cut(s) 88
BtgI CCRYGG 3 cut(s) 509, 557, 762
BtsCI GGATG 7 cut(s) 235, 355, 378, 445, 643, 707, 750
BtsI GCAGTG 1 cut(s) 414
BtsIMutI CAGTG 3 cut(s) 414, 428, 669
Cac8I GCNNGC 2 cut(s) 525, 773
CfoI GCGC 3 cut(s) 130, 525, 527
Cfr13I GGNCC 2 cut(s) 117, 451
Cfr9I CCCGGG 1 cut(s) 195
Csp6I GTAC 1 cut(s) 31
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 2 cut(s) 435, 612
DpnI GATC 2 cut(s) 110, 377
DpnII GATC 2 cut(s) 108, 375
Eam1104I CTCTTC 3 cut(s) 32, 323, 425
EarI CTCTTC 3 cut(s) 32, 323, 425
Eco130I CCWWGG 2 cut(s) 557, 762
Eco47I GGWCC 2 cut(s) 117, 451
Eco47III AGCGCT 1 cut(s) 129
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 1 cut(s) 195
EcoO109I RGGNCCY 1 cut(s) 451
EcoT14I CCWWGG 2 cut(s) 557, 762
EcoT22I ATGCAT 1 cut(s) 773
ErhI CCWWGG 2 cut(s) 557, 762
Fnu4HI GCNGC 1 cut(s) 463
FokI GGATG 7 cut(s) 242, 362, 365, 452, 630, 694, 737
Fsp4HI GCNGC 1 cut(s) 463
GlaI GCGC 3 cut(s) 129, 524, 526
GluI GCNGC 1 cut(s) 463
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 1 cut(s) 88
HapII CCGG 3 cut(s) 196, 455, 686
HhaI GCGC 3 cut(s) 130, 525, 527
Hin6I GCGC 3 cut(s) 128, 523, 525
HinP1I GCGC 3 cut(s) 128, 523, 525
HinfI GANTC 7 cut(s) 40, 144, 242, 256, 276, 656, 716
HpaII CCGG 3 cut(s) 196, 455, 686
HphI GGTGA 1 cut(s) 313
Hpy166II GTNNAC 1 cut(s) 603
Hpy188I TCNGA 4 cut(s) 261, 551, 615, 715
Hpy188III TCNNGA 3 cut(s) 154, 253, 382
Hpy8I GTNNAC 1 cut(s) 603
HpyAV CCTTC 2 cut(s) 476, 675
HpyCH4III ACNGT 2 cut(s) 432, 673
HpyCH4V TGCA 5 cut(s) 16, 164, 478, 501, 771
HpyF10VI GCNNNNNNNGC 1 cut(s) 94
HpyF3I CTNAG 2 cut(s) 435, 612
HspAI GCGC 3 cut(s) 128, 523, 525
Kzo9I GATC 2 cut(s) 108, 375
LmnI GCTCC 3 cut(s) 216, 287, 694
Lsp1109I GCAGC 1 cut(s) 474
MaeIII GTNAC 4 cut(s) 301, 398, 442, 757
MalI GATC 2 cut(s) 110, 377
MboI GATC 2 cut(s) 108, 375
MboII GAAGA 5 cut(s) 49, 310, 412, 580, 651
MfeI CAATTG 1 cut(s) 781
MluCI AATT 4 cut(s) 68, 424, 471, 781
MlyI GAGTC 3 cut(s) 49, 251, 710
MmeI TCCRAC 1 cut(s) 738
Mph1103I ATGCAT 1 cut(s) 773
MslI CAYNNNNRTG 1 cut(s) 102
MspI CCGG 3 cut(s) 196, 455, 686
MspR9I CCNGG 3 cut(s) 196, 197, 455
MunI CAATTG 1 cut(s) 781
Mva1269I GAATGC 1 cut(s) 391
MvnI CGCG 1 cut(s) 525
MwoI GCNNNNNNNGC 1 cut(s) 94
NciI CCSGG 3 cut(s) 196, 197, 455
NcoI CCATGG 2 cut(s) 557, 762
NdeII GATC 2 cut(s) 108, 375
NlaIV GGNNCC 3 cut(s) 212, 453, 690
NmuCI GTSAC 3 cut(s) 301, 398, 442
NsiI ATGCAT 1 cut(s) 773
NspI RCATGY 3 cut(s) 20, 31, 775
PaeI GCATGC 1 cut(s) 775
PalAI GGCGCGCC 1 cut(s) 523
PauI GCGCGC 1 cut(s) 523
PciI ACATGT 1 cut(s) 27
PctI GAATGC 1 cut(s) 391
PfeI GAWTC 4 cut(s) 144, 256, 276, 656
PkrI GCNGC 1 cut(s) 464
PleI GAGTC 3 cut(s) 48, 250, 710
PpsI GAGTC 3 cut(s) 48, 250, 710
PpuMI RGGWCCY 1 cut(s) 451
PscI ACATGT 1 cut(s) 27
Psp5II RGGWCCY 1 cut(s) 451
PspN4I GGNNCC 3 cut(s) 212, 453, 690
PspPI GGNCC 2 cut(s) 117, 451
PspPPI RGGWCCY 1 cut(s) 451
PstI CTGCAG 1 cut(s) 503
PteI GCGCGC 1 cut(s) 523
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
RseI CAYNNNNRTG 1 cut(s) 102
SatI GCNGC 1 cut(s) 463
Sau3AI GATC 2 cut(s) 108, 375
Sau96I GGNCC 2 cut(s) 117, 451
SchI GAGTC 3 cut(s) 49, 251, 710
ScrFI CCNGG 3 cut(s) 196, 197, 455
SetI ASST 4 cut(s) 152, 303, 613, 725
SfcI CTRYAG 2 cut(s) 499, 606
SgsI GGCGCGCC 1 cut(s) 523
SinI GGWCC 2 cut(s) 117, 451
SmaI CCCGGG 1 cut(s) 197
SmiMI CAYNNNNRTG 1 cut(s) 102
SphI GCATGC 1 cut(s) 775
Sse9I AATT 4 cut(s) 68, 424, 471, 781
SsiI CCGC 1 cut(s) 120
StyD4I CCNGG 3 cut(s) 194, 195, 453
StyI CCWWGG 2 cut(s) 557, 762
TaaI ACNGT 2 cut(s) 432, 673
TaqI TCGA 2 cut(s) 368, 731
TasI AATT 4 cut(s) 68, 424, 471, 781
TatI WGTACW 1 cut(s) 30
TfiI GAWTC 4 cut(s) 144, 256, 276, 656
TscAI CASTG 3 cut(s) 414, 435, 676
TseFI GTSAC 3 cut(s) 301, 398, 442
TseI GCWGC 1 cut(s) 462
Tsp45I GTSAC 3 cut(s) 301, 398, 442
TspDTI ATGAA 5 cut(s) 150, 310, 565, 696, 739
TspMI CCCGGG 1 cut(s) 195
TspRI CASTG 3 cut(s) 414, 435, 676
VpaK11BI GGWCC 2 cut(s) 117, 451
XapI RAATTY 1 cut(s) 68
XceI RCATGY 3 cut(s) 20, 31, 775
XmaI CCCGGG 1 cut(s) 195
Zsp2I ATGCAT 1 cut(s) 773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.