Rroxscaffold_3G00227600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003482
Physical Location & Seq
Reverse (-)
11794124 .. 11803234
9111 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00227600.1

Sequence Viewer

Length: 606 bp
ATGCGAACGACCTCGGACAAGCTAGCCGCTAAGTTCTATTTCCACGCTAGAAGCTTCTCGGAGATCTATAACAACGGCCTAACTCACCGCTTCGCCATTGCGATTATTGCCGCCGATGGCCAATCGTGTTCGGGAAGACGCCAATTATTGTGCGGGAGAAATATCATAAGCAATTGGCATAGCGGAGAATTGGGTTTTCAAGATTTGAAAAGTGAGTTTGCTACCTTTGAGCTTCGCAATAGTGCTTTTAGAACGGATGCCGAGAATCTCATCCGAGTTCCTCGGTTTCACACGACCATGATGCACGAAATTCGACCTAGTGTTATGGTTGGTCTCGGCTCCCATATAAAAGTGCTCCACAGGACCACATCATTCAGTATAATACATAACTCACTTGTTTGGTTTGTCACGTCACTTGAACAGGACATCGCGCGATTCGTAGGAATCTCTAATATCGAGGGGAATTTCCCCCCAATGGATTTCACAAAGAAAGATGTCGACATCTTCGACGAGGACGAAGATGATGAACCTAACAACCAAGATGGGACCATTCCTCCTCCCCTCTCATCCTCCTCCTCTAGCCGAAGCCGGTGGTGGCGTCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

201

Amino Acids

22.97

Weight (kDa)

6.71

Isoelectric Point (pI)

60.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 498
AccII CGCG 2 cut(s) 431, 433
AciI CCGC 5 cut(s) 27, 88, 111, 153, 183
AcoI YGGCCR 1 cut(s) 118
AcsI RAATTY 2 cut(s) 309, 463
AcyI GRCGYC 2 cut(s) 139, 598
AfiI CCNNNNNNNGG 1 cut(s) 475
AgsI TTSAA 3 cut(s) 200, 208, 419
AjiI CACGTC 1 cut(s) 411
AluBI AGCT 3 cut(s) 22, 54, 232
AluI AGCT 3 cut(s) 22, 54, 232
Alw21I GWGCWC 1 cut(s) 357
Alw26I GTCTC 1 cut(s) 338
AoxI GGCC 2 cut(s) 76, 118
ApoI RAATTY 2 cut(s) 309, 463
AspLEI GCGC 1 cut(s) 433
AspS9I GGNCC 2 cut(s) 363, 546
AsuHPI GGTGA 1 cut(s) 77
AsuNHI GCTAGC 1 cut(s) 22
AvaII GGWCC 2 cut(s) 363, 546
BalI TGGCCA 1 cut(s) 120
BbsI GAAGAC 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 357
BccI CCATC 2 cut(s) 110, 536
BceAI ACGGC 1 cut(s) 91
BcgI CGANNNNNNTGC 4 cut(s) 283, 293, 317, 327
BcoDI GTCTC 1 cut(s) 338
BfaI CTAG 4 cut(s) 23, 48, 318, 579
BglII AGATCT 1 cut(s) 63
BisI GCNGC 2 cut(s) 27, 111
BlsI GCNGC 2 cut(s) 28, 112
Bme18I GGWCC 2 cut(s) 363, 546
BmgBI CACGTC 1 cut(s) 411
BmgT120I GGNCC 2 cut(s) 363, 546
BmiI GGNNCC 2 cut(s) 340, 547
BmsI GCATC 2 cut(s) 247, 291
BmtI GCTAGC 1 cut(s) 26
BpiI GAAGAC 1 cut(s) 142
BsaHI GRCGYC 2 cut(s) 139, 598
BsaI GGTCTC 1 cut(s) 338
BsaJI CCNNGG 2 cut(s) 12, 281
BsaXI ACNNNNNCTCC 2 cut(s) 538, 568
Bsc4I CCNNNNNNNGG 1 cut(s) 475
Bse118I RCCGGY 1 cut(s) 588
Bse3DI GCAATG 1 cut(s) 96
BseDI CCNNGG 2 cut(s) 12, 281
BseGI GGATG 3 cut(s) 262, 270, 566
BseLI CCNNNNNNNGG 1 cut(s) 475
BseMI GCAATG 1 cut(s) 96
BseRI GAGGAG 3 cut(s) 546, 562, 565
Bsh1236I CGCG 2 cut(s) 431, 433
BshFI GGCC 2 cut(s) 78, 120
BsiHKAI GWGCWC 1 cut(s) 357
BsiSI CCGG 1 cut(s) 589
BslFI GGGAC 1 cut(s) 559
BslI CCNNNNNNNGG 1 cut(s) 475
BsmAI GTCTC 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 559
BsnI GGCC 2 cut(s) 78, 120
Bso31I GGTCTC 1 cut(s) 338
Bsp1286I GDGCHC 1 cut(s) 357
Bsp143I GATC 1 cut(s) 63
BspACI CCGC 5 cut(s) 27, 88, 111, 153, 183
BspANI GGCC 2 cut(s) 78, 120
BspFNI CGCG 2 cut(s) 431, 433
BspLI GGNNCC 2 cut(s) 340, 547
BspOI GCTAGC 1 cut(s) 26
BspTNI GGTCTC 1 cut(s) 338
BsrDI GCAATG 1 cut(s) 96
BsrFI RCCGGY 1 cut(s) 588
BssAI RCCGGY 1 cut(s) 588
BssECI CCNNGG 2 cut(s) 12, 281
BssMI GATC 1 cut(s) 63
BssNI GRCGYC 2 cut(s) 139, 598
BstACI GRCGYC 2 cut(s) 139, 598
BstC8I GCNNGC 1 cut(s) 24
BstDEI CTNAG 1 cut(s) 30
BstF5I GGATG 3 cut(s) 262, 270, 566
BstFNI CGCG 2 cut(s) 431, 433
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 1 cut(s) 66
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 1 cut(s) 63
BstMWI GCNNNNNNNGC 1 cut(s) 107
BstUI CGCG 2 cut(s) 431, 433
BstV2I GAAGAC 1 cut(s) 142
BstX2I RGATCY 1 cut(s) 63
BstYI RGATCY 1 cut(s) 63
BsuRI GGCC 2 cut(s) 78, 120
BtgZI GCGATG 1 cut(s) 412
BtrI CACGTC 1 cut(s) 411
BtsCI GGATG 3 cut(s) 262, 270, 566
Cac8I GCNNGC 1 cut(s) 24
CfoI GCGC 1 cut(s) 433
Cfr10I RCCGGY 1 cut(s) 588
Cfr13I GGNCC 2 cut(s) 363, 546
CseI GACGC 2 cut(s) 147, 587
CviAII CATG 1 cut(s) 298
CviJI RGCY 9 cut(s) 22, 26, 54, 78, 120, 232, 339, 582, 588
CviKI_1 RGCY 9 cut(s) 22, 26, 54, 78, 120, 232, 339, 582, 588
DdeI CTNAG 1 cut(s) 30
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
EaeI YGGCCR 1 cut(s) 118
Eco31I GGTCTC 1 cut(s) 338
Eco47I GGWCC 2 cut(s) 363, 546
FaeI CATG 1 cut(s) 301
FaiI YATR 9 cut(s) 69, 167, 180, 299, 326, 345, 347, 380, 387
FaqI GGGAC 1 cut(s) 559
FatI CATG 1 cut(s) 297
FauI CCCGC 1 cut(s) 146
FblI GTMKAC 1 cut(s) 498
Fnu4HI GCNGC 2 cut(s) 27, 111
FokI GGATG 3 cut(s) 257, 269, 553
Fsp4HI GCNGC 2 cut(s) 27, 111
FspBI CTAG 4 cut(s) 23, 48, 318, 579
GlaI GCGC 1 cut(s) 432
GluI GCNGC 2 cut(s) 27, 111
HaeIII GGCC 2 cut(s) 78, 120
HapII CCGG 1 cut(s) 589
HgaI GACGC 2 cut(s) 147, 587
HhaI GCGC 1 cut(s) 433
Hin1I GRCGYC 2 cut(s) 139, 598
Hin1II CATG 1 cut(s) 301
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HincII GTYRAC 1 cut(s) 499
HindII GTYRAC 1 cut(s) 499
HindIII AAGCTT 1 cut(s) 52
HinfI GANTC 3 cut(s) 265, 435, 444
HpaII CCGG 1 cut(s) 589
HphI GGTGA 1 cut(s) 77
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 3 cut(s) 16, 61, 275
Hpy188III TCNNGA 2 cut(s) 132, 200
Hpy8I GTNNAC 1 cut(s) 499
Hpy99I CGWCG 2 cut(s) 512, 603
HpyCH4IV ACGT 1 cut(s) 410
HpyCH4V TGCA 1 cut(s) 304
HpyF10VI GCNNNNNNNGC 1 cut(s) 107
HpyF3I CTNAG 1 cut(s) 30
HpySE526I ACGT 1 cut(s) 410
Hsp92I GRCGYC 2 cut(s) 139, 598
Hsp92II CATG 1 cut(s) 301
HspAI GCGC 1 cut(s) 431
Kzo9I GATC 1 cut(s) 63
LmnI GCTCC 2 cut(s) 344, 360
LpnPI CCDG 3 cut(s) 346, 407, 602
LweI GCATC 2 cut(s) 247, 291
MaeI CTAG 4 cut(s) 23, 48, 318, 579
MaeII ACGT 1 cut(s) 410
MaeIII GTNAC 2 cut(s) 406, 411
MalI GATC 1 cut(s) 65
MboI GATC 1 cut(s) 63
MboII GAAGA 3 cut(s) 147, 496, 530
MfeI CAATTG 1 cut(s) 172
MflI RGATCY 1 cut(s) 63
MhlI GDGCHC 1 cut(s) 357
MlsI TGGCCA 1 cut(s) 120
MluCI AATT 5 cut(s) 143, 172, 188, 309, 463
MluNI TGGCCA 1 cut(s) 120
Mox20I TGGCCA 1 cut(s) 120
MscI TGGCCA 1 cut(s) 120
MslI CAYNNNNRTG 1 cut(s) 296
Msp20I TGGCCA 1 cut(s) 120
MspI CCGG 1 cut(s) 589
MunI CAATTG 1 cut(s) 172
MvnI CGCG 2 cut(s) 431, 433
MwoI GCNNNNNNNGC 1 cut(s) 107
NdeII GATC 1 cut(s) 63
NheI GCTAGC 1 cut(s) 22
NlaIII CATG 1 cut(s) 301
NlaIV GGNNCC 2 cut(s) 340, 547
NmeAIII GCCGAG 2 cut(s) 286, 315
NmuCI GTSAC 2 cut(s) 406, 411
PcsI WCGNNNNNNNCGW 3 cut(s) 435, 504, 513
PfeI GAWTC 3 cut(s) 265, 435, 444
PkrI GCNGC 2 cut(s) 28, 112
PspN4I GGNNCC 2 cut(s) 340, 547
PspPI GGNCC 2 cut(s) 363, 546
PsuI RGATCY 1 cut(s) 63
RseI CAYNNNNRTG 1 cut(s) 296
SalI GTCGAC 1 cut(s) 497
SatI GCNGC 2 cut(s) 27, 111
Sau3AI GATC 1 cut(s) 63
Sau96I GGNCC 2 cut(s) 363, 546
SduI GDGCHC 1 cut(s) 357
SetI ASST 8 cut(s) 14, 24, 56, 227, 234, 319, 413, 532
SfaNI GCATC 2 cut(s) 247, 291
SinI GGWCC 2 cut(s) 363, 546
SmiMI CAYNNNNRTG 1 cut(s) 296
Sse9I AATT 5 cut(s) 143, 172, 188, 309, 463
SsiI CCGC 5 cut(s) 27, 88, 111, 153, 183
SspMI CTAG 4 cut(s) 23, 48, 318, 579
TaiI ACGT 1 cut(s) 413
TaqI TCGA 4 cut(s) 313, 456, 498, 507
TasI AATT 5 cut(s) 143, 172, 188, 309, 463
TauI GCSGC 2 cut(s) 29, 113
TfiI GAWTC 3 cut(s) 265, 435, 444
TseFI GTSAC 2 cut(s) 406, 411
Tsp45I GTSAC 2 cut(s) 406, 411
TspDTI ATGAA 1 cut(s) 540
TspGWI ACGGA 1 cut(s) 269
VpaK11BI GGWCC 2 cut(s) 363, 546
XapI RAATTY 2 cut(s) 309, 463
XmiI GTMKAC 1 cut(s) 498
XspI CTAG 4 cut(s) 23, 48, 318, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.