Rorug01G0236000

histidine-containing phosphotransfer protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0002158
Physical Location & Seq
Reverse (-)
34052116 .. 34052589
474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0236000.1

Sequence Viewer

Length: 474 bp
ATGGAAATTCTTAGCTGGAATTGCAGAGGCATCTGCAACGACGCTACTAAAAGGGCTTTGAAAGATTTAATCGTCCAAAACAAGCCTCAAATTGTGTTTCTATGTGAGACAAAGATTAGCAGGCAGGAAGATTTTCGGTCCCTGCATCATGCCTTAGGGTTTCCGAACTCAAAAGAGGTGCTTAGTGAGGGACAGGCCGACGGATTAGGTCTGTTTTGGAGTGGAGAAATCGATATGAGGGTTCGTACCAGATCTGCACATCACATCAATGCGGTAGTTCATGGTGGGCCTGGCGATTCTCGATGGCGACTAACAGGGTTTTATGGTTATGCACGTACCAGTGATAGGGAACGATCTTGGCAACTGTTGAAGGAACTGGGGGATCTCGACTCACTGCCGTGGGTAGTGATTGGGGACTTCAATGAGATCCTCAACAATGGTGAGAAACTCGATGGACCACCTAGAGCAGAATGA

Protein Analysis

157

Amino Acids

17.79

Weight (kDa)

6.21

Isoelectric Point (pI)

40.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 4 - 152 9.8e-14 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 272
AclWI GGATC 2 cut(s) 390, 421
AcsI RAATTY 1 cut(s) 6
AfaI GTAC 2 cut(s) 247, 337
AfiI CCNNNNNNNGG 1 cut(s) 345
AgsI TTSAA 3 cut(s) 61, 370, 421
AjnI CCWGG 1 cut(s) 289
AleI CACNNNNGTG 1 cut(s) 397
AluBI AGCT 1 cut(s) 15
AluI AGCT 1 cut(s) 15
Alw26I GTCTC 1 cut(s) 101
AlwI GGATC 2 cut(s) 390, 421
AoxI GGCC 2 cut(s) 195, 287
ApoI RAATTY 1 cut(s) 6
Asp700I GAANNNNTTC 1 cut(s) 132
AspS9I GGNCC 3 cut(s) 138, 287, 455
AsuHPI GGTGA 1 cut(s) 452
AvaII GGWCC 2 cut(s) 138, 455
AxyI CCTNAGG 1 cut(s) 154
BccI CCATC 2 cut(s) 297, 446
BceAI ACGGC 1 cut(s) 382
BciT130I CCWGG 1 cut(s) 291
BcoDI GTCTC 1 cut(s) 101
BfaI CTAG 1 cut(s) 462
BglII AGATCT 1 cut(s) 251
Bme1390I CCNGG 1 cut(s) 291
Bme18I GGWCC 2 cut(s) 138, 455
BmgT120I GGNCC 3 cut(s) 138, 287, 455
BmiI GGNNCC 1 cut(s) 140
BmrFI CCNGG 1 cut(s) 291
BmrI ACTGGG 1 cut(s) 386
BmsI GCATC 2 cut(s) 39, 154
BmuI ACTGGG 1 cut(s) 386
Bsa29I ATCGAT 1 cut(s) 231
BsaAI YACGTR 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 398
Bsc4I CCNNNNNNNGG 1 cut(s) 345
Bse1I ACTGG 2 cut(s) 339, 381
Bse21I CCTNAGG 1 cut(s) 154
BseBI CCWGG 1 cut(s) 291
BseCI ATCGAT 1 cut(s) 231
BseDI CCNNGG 1 cut(s) 398
BseLI CCNNNNNNNGG 1 cut(s) 345
BseNI ACTGG 2 cut(s) 339, 381
BsgI GTGCAG 1 cut(s) 240
BshFI GGCC 2 cut(s) 197, 289
BshVI ATCGAT 1 cut(s) 231
BslFI GGGAC 3 cut(s) 124, 204, 428
BslI CCNNNNNNNGG 1 cut(s) 345
BsmAI GTCTC 1 cut(s) 101
BsmFI GGGAC 3 cut(s) 124, 204, 428
BsnI GGCC 2 cut(s) 197, 289
Bsp143I GATC 4 cut(s) 251, 353, 382, 426
BspACI CCGC 1 cut(s) 272
BspANI GGCC 2 cut(s) 197, 289
BspDI ATCGAT 1 cut(s) 231
BspLI GGNNCC 1 cut(s) 140
BspPI GGATC 2 cut(s) 390, 421
BsrI ACTGG 2 cut(s) 339, 381
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 4 cut(s) 251, 353, 382, 426
Bst2UI CCWGG 1 cut(s) 291
Bst4CI ACNGT 1 cut(s) 366
BstBAI YACGTR 1 cut(s) 335
BstC8I GCNNGC 1 cut(s) 122
BstDEI CTNAG 3 cut(s) 11, 154, 182
BstDSI CCRYGG 1 cut(s) 398
BstKTI GATC 4 cut(s) 254, 356, 385, 429
BstMAI GTCTC 1 cut(s) 101
BstMBI GATC 4 cut(s) 251, 353, 382, 426
BstMWI GCNNNNNNNGC 1 cut(s) 21
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstX2I RGATCY 3 cut(s) 251, 382, 426
BstYI RGATCY 3 cut(s) 251, 382, 426
Bsu15I ATCGAT 1 cut(s) 231
Bsu36I CCTNAGG 1 cut(s) 154
BsuRI GGCC 2 cut(s) 197, 289
BsuTUI ATCGAT 1 cut(s) 231
BtgI CCRYGG 1 cut(s) 398
BtsI GCAGTG 1 cut(s) 392
BtsIMutI CAGTG 2 cut(s) 346, 392
Cac8I GCNNGC 1 cut(s) 122
Cfr13I GGNCC 3 cut(s) 138, 287, 455
ClaI ATCGAT 1 cut(s) 231
CseI GACGC 1 cut(s) 50
Csp6I GTAC 2 cut(s) 246, 336
CviAII CATG 2 cut(s) 149, 281
CviJI RGCY 5 cut(s) 15, 56, 85, 197, 289
CviKI_1 RGCY 5 cut(s) 15, 56, 85, 197, 289
CviQI GTAC 2 cut(s) 246, 336
DdeI CTNAG 3 cut(s) 11, 154, 182
DpnI GATC 4 cut(s) 253, 355, 384, 428
DpnII GATC 4 cut(s) 251, 353, 382, 426
Eco47I GGWCC 2 cut(s) 138, 455
Eco81I CCTNAGG 1 cut(s) 154
EcoRII CCWGG 1 cut(s) 289
FaeI CATG 2 cut(s) 152, 284
FaiI YATR 6 cut(s) 103, 150, 236, 282, 324, 330
FalI AAGNNNNNCTT 2 cut(s) 165, 197
FaqI GGGAC 3 cut(s) 124, 204, 428
FatI CATG 2 cut(s) 148, 280
FspBI CTAG 1 cut(s) 462
HaeIII GGCC 2 cut(s) 197, 289
HgaI GACGC 1 cut(s) 50
Hin1II CATG 2 cut(s) 152, 284
HinfI GANTC 2 cut(s) 296, 389
HphI GGTGA 1 cut(s) 452
Hpy188I TCNGA 1 cut(s) 165
Hpy188III TCNNGA 2 cut(s) 300, 386
Hpy99I CGWCG 2 cut(s) 44, 203
HpyAV CCTTC 1 cut(s) 364
HpyCH4III ACNGT 1 cut(s) 366
HpyCH4IV ACGT 1 cut(s) 334
HpyCH4V TGCA 5 cut(s) 24, 36, 145, 257, 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 21
HpyF3I CTNAG 3 cut(s) 11, 154, 182
HpySE526I ACGT 1 cut(s) 334
Hsp92II CATG 2 cut(s) 152, 284
Kzo9I GATC 4 cut(s) 251, 353, 382, 426
LweI GCATC 2 cut(s) 39, 154
MaeI CTAG 1 cut(s) 462
MaeII ACGT 1 cut(s) 334
MalI GATC 4 cut(s) 253, 355, 384, 428
MboI GATC 4 cut(s) 251, 353, 382, 426
MboII GAAGA 1 cut(s) 140
MflI RGATCY 3 cut(s) 251, 382, 426
MluCI AATT 3 cut(s) 6, 19, 90
MlyI GAGTC 1 cut(s) 383
MnlI CCTC 6 cut(s) 20, 96, 169, 181, 231, 440
MroXI GAANNNNTTC 1 cut(s) 132
MseI TTAA 1 cut(s) 68
MslI CAYNNNNRTG 2 cut(s) 267, 397
MspR9I CCNGG 1 cut(s) 291
MvaI CCWGG 1 cut(s) 291
MwoI GCNNNNNNNGC 1 cut(s) 21
NdeII GATC 4 cut(s) 251, 353, 382, 426
NlaIII CATG 2 cut(s) 152, 284
NlaIV GGNNCC 1 cut(s) 140
OliI CACNNNNGTG 1 cut(s) 397
PdmI GAANNNNTTC 1 cut(s) 132
PfeI GAWTC 1 cut(s) 296
PleI GAGTC 1 cut(s) 383
PpsI GAGTC 1 cut(s) 383
Ppu21I YACGTR 1 cut(s) 335
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 140
PspPI GGNCC 3 cut(s) 138, 287, 455
PsuI RGATCY 3 cut(s) 251, 382, 426
RsaI GTAC 2 cut(s) 247, 337
RsaNI GTAC 2 cut(s) 246, 336
RseI CAYNNNNRTG 2 cut(s) 267, 397
SaqAI TTAA 1 cut(s) 68
Sau3AI GATC 4 cut(s) 251, 353, 382, 426
Sau96I GGNCC 3 cut(s) 138, 287, 455
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 1 cut(s) 291
SetI ASST 5 cut(s) 17, 180, 211, 337, 463
SfaNI GCATC 2 cut(s) 39, 154
SinI GGWCC 2 cut(s) 138, 455
SmiMI CAYNNNNRTG 2 cut(s) 267, 397
Sse9I AATT 3 cut(s) 6, 19, 90
SsiI CCGC 1 cut(s) 272
SspMI CTAG 1 cut(s) 462
StyD4I CCNGG 1 cut(s) 289
TaaI ACNGT 1 cut(s) 366
TaiI ACGT 1 cut(s) 337
TaqI TCGA 4 cut(s) 231, 301, 387, 450
TaqII GACCGA 1 cut(s) 126
TasI AATT 3 cut(s) 6, 19, 90
TfiI GAWTC 1 cut(s) 296
Tru1I TTAA 1 cut(s) 68
Tru9I TTAA 1 cut(s) 68
TscAI CASTG 2 cut(s) 346, 399
TspDTI ATGAA 1 cut(s) 269
TspGWI ACGGA 1 cut(s) 216
TspRI CASTG 2 cut(s) 346, 399
VpaK11BI GGWCC 2 cut(s) 138, 455
XapI RAATTY 1 cut(s) 6
XmnI GAANNNNTTC 1 cut(s) 132
XspI CTAG 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.