Rorug02G0221700

Transketolase, pyrimidine binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0002158
Physical Location & Seq
Reverse (-)
21446666 .. 21447472
807 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0221700.1

Sequence Viewer

Length: 807 bp
ATGGCATTGGATGTTGCTGAATTCCTGTGGAACAAGTTCTTGAAGTCTCTAGCAGAGCTAGAGTCTGAATCTAATTCAGGTTCTATTACCATCTCTGTCTTCAAATCATCTATAAAAGATCTCGTGTCTGATTTCAAAGCAATCAAAGATGTTGTGAATGAGAGAGATATAATGCCGAAACCTTATACCTACTCTGAAACTATCGATGTGCTCTACAATAATCTCAACGATGCATTGGCTGAGTGCCGAGTCTTCGCACATGAGTGCATGGAGATTACTGATATGTACTCACGCTTCAACTTTCGAAGATTTACTTTCATCTCAAAGATAAAGAGTCGGCTGGCCAAAATAAAACAGGAGCTTGAGCAATTGCCGTTGCGGCAAAATGGTGGCAATCCTCTGGAAAAGACGGGAGTCACCAGACCTAAACTTTCAGCAGAAGTTTTGGGATTTGATAAGCAATCAGAGGAGATTGAGAACTTACTACTACTTGAAGCAGAGGGTTCCAGTTCTAGTAGTGCTGGTAAATTGACGGCAATTGGGGTCGTTGGTATTGCTGGAGTGGGTAAGACCACCCTGGTTCAGAAGGTTTTGAAGAGCGACGGAGTGCAGAAGAAGTTTAATCATATACTGTGGCTACCCTTTGAAGGAGAACATCAATATAGTAGGTTTAGCTTTGAAACATGTATCTTTGATGGTAATGACAAAATCATAGAGGCGAGGATTGTTGGCCTTGATAAGCTACTGGAAAAGCTAAGCCGCCTGCTGTTATCAGGAAAATGTACGTTATTTGATTGTGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transket_pyr Transketolase_C

Protein Analysis

268

Amino Acids

30.05

Weight (kDa)

6.04

Isoelectric Point (pI)

40.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 177 - 215 2.3e-07 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 379, 760
AcoI YGGCCR 1 cut(s) 342
AcsI RAATTY 1 cut(s) 20
AfaI GTAC 2 cut(s) 287, 784
AfiI CCNNNNNNNGG 1 cut(s) 647
AflIII ACRYGT 1 cut(s) 683
AgsI TTSAA 8 cut(s) 43, 103, 136, 298, 494, 595, 647, 680
AjnI CCWGG 1 cut(s) 576
AleI CACNNNNGTG 1 cut(s) 262
AluBI AGCT 5 cut(s) 58, 361, 675, 742, 754
AluI AGCT 5 cut(s) 58, 361, 675, 742, 754
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 1 cut(s) 51
AoxI GGCC 2 cut(s) 342, 730
ApoI RAATTY 1 cut(s) 20
Asp700I GAANNNNTTC 1 cut(s) 35
AsuHPI GGTGA 1 cut(s) 409
AsuII TTCGAA 1 cut(s) 304
BalI TGGCCA 1 cut(s) 344
BarI GAAGNNNNNNTAC 2 cut(s) 278, 310
BauI CACGAG 1 cut(s) 122
BbsI GAAGAC 2 cut(s) 91, 244
Bbv12I GWGCWC 1 cut(s) 213
BccI CCATC 2 cut(s) 98, 689
BceAI ACGGC 2 cut(s) 358, 549
BciT130I CCWGG 1 cut(s) 578
BcoDI GTCTC 1 cut(s) 51
BfaI CTAG 3 cut(s) 50, 59, 513
BglI GCCNNNNNGGC 1 cut(s) 379
BglII AGATCT 1 cut(s) 118
BisI GCNGC 2 cut(s) 380, 760
BlpI GCTNAGC 1 cut(s) 755
BlsI GCNGC 2 cut(s) 381, 761
Bme1390I CCNGG 1 cut(s) 578
BmiI GGNNCC 1 cut(s) 505
BmrFI CCNGG 1 cut(s) 578
BmsI GCATC 1 cut(s) 220
BoxI GACNNNNGTC 1 cut(s) 413
BpiI GAAGAC 2 cut(s) 91, 244
BpmI CTGGAG 1 cut(s) 579
Bpu1102I GCTNAGC 1 cut(s) 755
Bpu14I TTCGAA 1 cut(s) 304
BpuEI CTTGAG 1 cut(s) 383
Bsa29I ATCGAT 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 576
Bsc4I CCNNNNNNNGG 1 cut(s) 647
Bse1I ACTGG 2 cut(s) 507, 750
BseBI CCWGG 1 cut(s) 578
BseCI ATCGAT 1 cut(s) 204
BseDI CCNNGG 1 cut(s) 576
BseGI GGATG 1 cut(s) 16
BseLI CCNNNNNNNGG 1 cut(s) 647
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 2 cut(s) 507, 750
BseRI GAGGAG 1 cut(s) 482
BsgI GTGCAG 1 cut(s) 629
BshFI GGCC 2 cut(s) 344, 732
BshVI ATCGAT 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 213
BslI CCNNNNNNNGG 1 cut(s) 647
BsmAI GTCTC 1 cut(s) 51
BsnI GGCC 2 cut(s) 344, 732
Bsp119I TTCGAA 1 cut(s) 304
Bsp1286I GDGCHC 1 cut(s) 213
Bsp143I GATC 1 cut(s) 118
Bsp1720I GCTNAGC 1 cut(s) 755
BspACI CCGC 2 cut(s) 379, 760
BspANI GGCC 2 cut(s) 344, 732
BspCNI CTCAG 1 cut(s) 232
BspDI ATCGAT 1 cut(s) 204
BspLI GGNNCC 1 cut(s) 505
BspQI GCTCTTC 1 cut(s) 590
BspT104I TTCGAA 1 cut(s) 304
BsrI ACTGG 2 cut(s) 507, 750
BssECI CCNNGG 1 cut(s) 576
BssMI GATC 1 cut(s) 118
BssSI CACGAG 1 cut(s) 122
Bst2BI CACGAG 1 cut(s) 122
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 1 cut(s) 633
Bst6I CTCTTC 1 cut(s) 590
BstBI TTCGAA 1 cut(s) 304
BstC8I GCNNGC 2 cut(s) 342, 764
BstDEI CTNAG 2 cut(s) 240, 755
BstF5I GGATG 1 cut(s) 16
BstKTI GATC 1 cut(s) 121
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 1 cut(s) 118
BstMWI GCNNNNNNNGC 1 cut(s) 379
BstNI CCWGG 1 cut(s) 578
BstNSI RCATGY 1 cut(s) 687
BstPAI GACNNNNGTC 1 cut(s) 413
BstSCI CCNGG 1 cut(s) 576
BstV2I GAAGAC 2 cut(s) 91, 244
BstX2I RGATCY 1 cut(s) 118
BstYI RGATCY 1 cut(s) 118
Bsu15I ATCGAT 1 cut(s) 204
BsuRI GGCC 2 cut(s) 344, 732
BsuTUI ATCGAT 1 cut(s) 204
BtsCI GGATG 1 cut(s) 16
Cac8I GCNNGC 2 cut(s) 342, 764
ClaI ATCGAT 1 cut(s) 204
Csp6I GTAC 2 cut(s) 286, 783
CviAII CATG 3 cut(s) 260, 268, 684
CviQI GTAC 2 cut(s) 286, 783
DdeI CTNAG 2 cut(s) 240, 755
DpnI GATC 1 cut(s) 120
DpnII GATC 1 cut(s) 118
EaeI YGGCCR 1 cut(s) 342
Eam1104I CTCTTC 1 cut(s) 590
EarI CTCTTC 1 cut(s) 590
EcoRI GAATTC 1 cut(s) 20
EcoRII CCWGG 1 cut(s) 576
EcoT22I ATGCAT 1 cut(s) 235
FaeI CATG 3 cut(s) 263, 271, 687
FalI AAGNNNNNCTT 2 cut(s) 298, 330
FatI CATG 3 cut(s) 259, 267, 683
Fnu4HI GCNGC 2 cut(s) 380, 760
FokI GGATG 1 cut(s) 23
Fsp4HI GCNGC 2 cut(s) 380, 760
FspBI CTAG 3 cut(s) 50, 59, 513
GluI GCNGC 2 cut(s) 380, 760
GsuI CTGGAG 1 cut(s) 579
HaeIII GGCC 2 cut(s) 344, 732
Hin1II CATG 3 cut(s) 263, 271, 687
HinfI GANTC 5 cut(s) 62, 68, 249, 334, 414
HphI GGTGA 1 cut(s) 409
Hpy188I TCNGA 5 cut(s) 67, 130, 196, 466, 585
Hpy188III TCNNGA 3 cut(s) 40, 401, 774
Hpy99I CGWCG 1 cut(s) 605
HpyAV CCTTC 2 cut(s) 580, 641
HpyCH4III ACNGT 1 cut(s) 633
HpyCH4IV ACGT 1 cut(s) 785
HpyCH4V TGCA 3 cut(s) 233, 267, 610
HpyF10VI GCNNNNNNNGC 1 cut(s) 379
HpyF3I CTNAG 2 cut(s) 240, 755
HpySE526I ACGT 1 cut(s) 785
Hsp92II CATG 3 cut(s) 263, 271, 687
Kzo9I GATC 1 cut(s) 118
LguI GCTCTTC 1 cut(s) 590
LmnI GCTCC 1 cut(s) 358
LweI GCATC 1 cut(s) 220
MaeI CTAG 3 cut(s) 50, 59, 513
MaeII ACGT 1 cut(s) 785
MaeIII GTNAC 1 cut(s) 415
MalI GATC 1 cut(s) 120
MboI GATC 1 cut(s) 118
MboII GAAGA 5 cut(s) 91, 244, 318, 607, 625
MfeI CAATTG 2 cut(s) 368, 537
MflI RGATCY 1 cut(s) 118
MhlI GDGCHC 1 cut(s) 213
MlsI TGGCCA 1 cut(s) 344
MluCI AATT 5 cut(s) 20, 73, 368, 527, 537
MluNI TGGCCA 1 cut(s) 344
MlyI GAGTC 4 cut(s) 71, 258, 343, 423
MmeI TCCRAC 1 cut(s) 781
MnlI CCTC 5 cut(s) 408, 460, 493, 709, 714
Mox20I TGGCCA 1 cut(s) 344
Mph1103I ATGCAT 1 cut(s) 235
MroXI GAANNNNTTC 1 cut(s) 35
MscI TGGCCA 1 cut(s) 344
MseI TTAA 1 cut(s) 621
MslI CAYNNNNRTG 1 cut(s) 262
Msp20I TGGCCA 1 cut(s) 344
MspR9I CCNGG 1 cut(s) 578
MunI CAATTG 2 cut(s) 368, 537
MvaI CCWGG 1 cut(s) 578
MwoI GCNNNNNNNGC 1 cut(s) 379
NdeII GATC 1 cut(s) 118
NlaIII CATG 3 cut(s) 263, 271, 687
NlaIV GGNNCC 1 cut(s) 505
NmeAIII GCCGAG 1 cut(s) 272
NmuCI GTSAC 1 cut(s) 415
NsiI ATGCAT 1 cut(s) 235
NspI RCATGY 1 cut(s) 687
NspV TTCGAA 1 cut(s) 304
OliI CACNNNNGTG 1 cut(s) 262
PciI ACATGT 1 cut(s) 683
PciSI GCTCTTC 1 cut(s) 590
PdmI GAANNNNTTC 1 cut(s) 35
PfeI GAWTC 1 cut(s) 68
PkrI GCNGC 2 cut(s) 381, 761
PleI GAGTC 4 cut(s) 70, 257, 342, 422
PpsI GAGTC 4 cut(s) 70, 257, 342, 422
PscI ACATGT 1 cut(s) 683
PshAI GACNNNNGTC 1 cut(s) 413
Psp6I CCWGG 1 cut(s) 576
PspGI CCWGG 1 cut(s) 576
PspN4I GGNNCC 1 cut(s) 505
PsuI RGATCY 1 cut(s) 118
RsaI GTAC 2 cut(s) 287, 784
RsaNI GTAC 2 cut(s) 286, 783
RseI CAYNNNNRTG 1 cut(s) 262
SapI GCTCTTC 1 cut(s) 590
SaqAI TTAA 1 cut(s) 621
SatI GCNGC 2 cut(s) 380, 760
Sau3AI GATC 1 cut(s) 118
SchI GAGTC 4 cut(s) 71, 258, 343, 423
ScrFI CCNGG 1 cut(s) 578
SduI GDGCHC 1 cut(s) 213
SfaNI GCATC 1 cut(s) 220
SfuI TTCGAA 1 cut(s) 304
SmiMI CAYNNNNRTG 1 cut(s) 262
SmlI CTYRAG 1 cut(s) 362
SmoI CTYRAG 1 cut(s) 362
Sse9I AATT 5 cut(s) 20, 73, 368, 527, 537
SsiI CCGC 2 cut(s) 379, 760
SspMI CTAG 3 cut(s) 50, 59, 513
StyD4I CCNGG 1 cut(s) 576
TaaI ACNGT 1 cut(s) 633
TaiI ACGT 1 cut(s) 788
TaqI TCGA 2 cut(s) 204, 304
TasI AATT 5 cut(s) 20, 73, 368, 527, 537
TatI WGTACW 1 cut(s) 285
TauI GCSGC 2 cut(s) 382, 762
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 1 cut(s) 621
Tru9I TTAA 1 cut(s) 621
TseFI GTSAC 1 cut(s) 415
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 1 cut(s) 307
TspGWI ACGGA 1 cut(s) 618
XapI RAATTY 1 cut(s) 20
XceI RCATGY 1 cut(s) 687
XmnI GAANNNNTTC 1 cut(s) 35
XspI CTAG 3 cut(s) 50, 59, 513
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.