Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000007

Orthogroup ID

OG0001215

Physical Location & Seq

Genomic Coordinates Reverse (-)

14010151 .. 14049339

Gene Structure 39189 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug07G0172000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1512 bp

ATGGAGTTGGATAGCATTCTAGGATATCTGGAGAGCAAGACCATCTTAGTTACTGGAGCCACTGGCTTACTAGGAATGGTATTTGTGGAGAAGATACTAAGGGTTCAACCAAATATTGGAAAGCTCTACCTTCTTATAAGAGCTTCTGATGCCAACTCTGCCACACATCGCATGCGCAATGAGATTATAGAGAAAGAGTTATTTAGGGTTCTTAGAGAAAAATGGGGCACAGATTTTGAATCCTTCATTTCAAAAAAAGTAGTTGCTATTTCGGGGGACGTAGCTTCTGCAGAACTAGGAGTGAAAGGATTTGAATTGAGGGAACGTATGTGCAATGAAATACAAATTATACTTCATTCTGCAGCAACCGTGGATTTTAATGACAGGTATGATATTGCTTTGGATGTGAATACATTTGGGGTTCTGCATGTGTTGAGCTTTGCGAAGAAGTGTTTGAAACTAGAAATGCTTGTTCACATATCAACTGCTTATGTGGGCGTCGGAAGGATTGGGCTTATACTGGAGGACTCCTCTCCAATGGATGAGTTGAGAAAGGAAATCACTAAATCTGACTTGAAAGTACTGGAAAGGAACATGGTGGTGGAAAAATTAAATGAACTAAAAGCACAAAATGCTTCAGAAAAAGTTATTACGAATAAGATGAAGGACTTGGGCTTTGCAAGGTCAAAGTTGTATGGTTGGCCAAGTACTTATTTGTTTACAAAAGCGATGGCAGAAGTGTATCTTGAACAATCAAAAGGTAATTTACCCATTACCATCATACGCACAGCTACAGTTACGAGCACTTACAAGGAACCATTTCCAGGTTGGCTTCAAGGTTACAGAACCGTTGATAGCCTAATTGGTACTTTTTGGAAGGGGGCACTGACATGTTTGCCTATGGATCCTACAGCTGTATTGGATTTGATTCCAGTTGATATGGTGGCCAATTCAATAATTACGGCGATGGTAGTGAATGCCAACAAATATCTTGAAAATCCTGGAATGATTTATCATGTGGGAACGTCCTTGAGAAATCCAATTAAAATGTCTGAGTGGACAAATTTTATGTTCCAATACTGCACCAAAAATCCATGGCTTTATAAGGATGGAATCCCGATCAAGGTTGAGAAGTTGAAAATGTTCACAACTATGGCTGCATTTCGCTCGTACATGCAAATCCGCTACATGTTACCATTGGAGGTATTAAAGTTCTTGAATAAAGAATTTGACCAATATTTTCAAGAATTATATGCCAACTATAACCGAAAAATCACATTTGCGATGCGGTTGGCTGAGATATACCGACCATTTACGCTATATAAGGGCATCTTTGACGACACCAATTTGGAGAAGTTGCGAAGGATAGCAACAGAAAATTACAGAGACGCTGAAATTTTTAATTTTGATCCAAGATGCATTGATTGGGAAGACTACTTGATGCGTACTCACATCCCTGGCCTTCGGAAACATGTTATGATCAAACATAGCCAGAGCCAGAGCCGACTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0006810 GO:0008150 GO:0012505 GO:0015291 GO:0015297 GO:0015780 GO:0015931 GO:0022804 GO:0022857 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234 GO:0055085 GO:0071702 GO:0071705 GO:0090480 GO:1901264

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

TPT

Protein Analysis

503

Amino Acids

57.98

Weight (kDa)

8.93

Isoelectric Point (pI)

34.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NAD_binding_4	PF07993	17 - 319	7.4e-68	Male sterility protein
Sterile	PF03015	396 - 493	4.9e-17	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001215)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21070 AT1G76670 AT1G76670 AT5G42420
fragaria_vesca	FvH4_2g17260 FvH4_2g17260 FvH4_5g26900 FvH4_5g26900
malus_domestica	MD05G1151400.v1.1 MD10G1145600.v1.1
prunus_persica	Prupe.8G184000_v2.0.a1 Prupe.8G184000_v2.0.a1 Prupe.8G184100_v2.0.a1
pyrus_communis	pycom05g14590
rosa_chinensis	RchiOBHm_Chr5g0006941 RchiOBHm_Chr5g0007051 RchiOBHm_Chr5g0007201 RchiOBHm_Chr5g0007361 RchiOBHm_Chr6g0281761 RchiOBHm_Chr7g0217841 RchiOBHm_Chr7g0217851 RchiOBHm_Chr7g0217941
rosa_laevigata	RLG00000002431 RLG00000012937
rosa_multiflora	Rmu_co8042740.1_g000001 Rmu_sc0002566.1_g000002 Rmu_sc0004530.1_g000002 Rmu_sc0012998.1_g000001 Rmu_sc0014282.1_g000009 Rmu_ssc0000126.1_g000047
rosa_roxburghii	Rroxscaffold_3G00241620 Rroxscaffold_3G00241650 Rroxscaffold_7G00186820
rosa_rugosa	Rorug04G0426000 Rorug06G0143500 Rorug07G0171700 Rorug07G0171800 Rorug07G0171900 Rorug07G0172000
rosa_samantha	Rh5CG061300 Rh6AG256200 Rh6BG259100 Rh6CG258100 Rh6DG250200 Rh7AG313000 Rh7AG313500 Rh7AG314400 Rh7BG304500
rosa_wichuraiana	Rw0G017280 Rw5G005110 Rw5G005150 Rw6G022210 Rw7G026510 Rw7G026540 Rw7G026580 Rw7G028220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	137, 1104
Acc16I	TGCGCA	1 cut(s)	176
AccB7I	CCANNNNNTGG	1 cut(s)	116
AciI	CCGC	2 cut(s)	1183, 1288
AclWI	GGATC	3 cut(s)	899, 912, 1403
AcoI	YGGCCR	2 cut(s)	701, 945
AcsI	RAATTY	3 cut(s)	1063, 1226, 1395
AcuI	CTGAAG	1 cut(s)	621
AcyI	GRCGYC	1 cut(s)	498
AfaI	GTAC	5 cut(s)	582, 709, 868, 1172, 1447
AfiI	CCNNNNNNNGG	3 cut(s)	116, 824, 1123
AflIII	ACRYGT	3 cut(s)	890, 1188, 1471
AjnI	CCWGG	3 cut(s)	823, 1000, 1456
AluBI	AGCT	6 cut(s)	124, 143, 284, 438, 791, 914
AluI	AGCT	6 cut(s)	124, 143, 284, 438, 791, 914
Alw21I	GWGCWC	1 cut(s)	806
Alw26I	GTCTC	1 cut(s)	1380
AlwI	GGATC	3 cut(s)	899, 912, 1403
AoxI	GGCC	3 cut(s)	701, 945, 1459
ApeKI	GCWGC	2 cut(s)	362, 1157
ApoI	RAATTY	3 cut(s)	1063, 1226, 1395
ArsI	GACNNNNNNTTYG	2 cut(s)	659, 691
Asp700I	GAANNNNTTC	2 cut(s)	819, 1142
AspLEI	GCGC	1 cut(s)	177
BaeGI	GKGCMC	2 cut(s)	230, 886
BalI	TGGCCA	2 cut(s)	703, 947
BamHI	GGATCC	1 cut(s)	904
BbsI	GAAGAC	1 cut(s)	1437
Bbv12I	GWGCWC	1 cut(s)	806
BbvI	GCAGC	2 cut(s)	374, 1144
BccI	CCATC	5 cut(s)	50, 724, 785, 961, 1103
BceAI	ACGGC	1 cut(s)	978
BcgI	CGANNNNNNTGC	2 cut(s)	1149, 1183
BciT130I	CCWGG	3 cut(s)	825, 1002, 1458
BclI	TGATCA	1 cut(s)	1479
BcoDI	GTCTC	1 cut(s)	1380
BfaI	CTAG	4 cut(s)	20, 71, 296, 461
BfmI	CTRYAG	4 cut(s)	288, 360, 792, 909
BisI	GCNGC	2 cut(s)	363, 1158
BlsI	GCNGC	2 cut(s)	364, 1159
BmcAI	AGTACT	2 cut(s)	582, 709
Bme1390I	CCNGG	3 cut(s)	825, 1002, 1458
BmiI	GGNNCC	3 cut(s)	58, 816, 906
BmrFI	CCNGG	3 cut(s)	825, 1002, 1458
BmsI	GCATC	5 cut(s)	139, 1275, 1338, 1406, 1431
BpiI	GAAGAC	1 cut(s)	1437
BplI	GAGNNNNNCTC	2 cut(s)	515, 547
BpmI	CTGGAG	3 cut(s)	50, 75, 542
BpuEI	CTTGAG	1 cut(s)	1051
BsaHI	GRCGYC	1 cut(s)	498
BsaJI	CCNNGG	3 cut(s)	369, 1094, 1456
Bsc4I	CCNNNNNNNGG	3 cut(s)	116, 824, 1123
Bse1I	ACTGG	5 cut(s)	58, 67, 525, 588, 932
Bse3DI	GCAATG	2 cut(s)	184, 340
BseBI	CCWGG	3 cut(s)	825, 1002, 1458
BseDI	CCNNGG	3 cut(s)	369, 1094, 1456
BseGI	GGATG	4 cut(s)	409, 547, 1114, 1452
BseLI	CCNNNNNNNGG	3 cut(s)	116, 824, 1123
BseMI	GCAATG	2 cut(s)	184, 340
BseMII	CTCAG	2 cut(s)	1044, 1287
BseNI	ACTGG	5 cut(s)	58, 67, 525, 588, 932
BseRI	GAGGAG	1 cut(s)	520
BseSI	GKGCMC	2 cut(s)	230, 886
BseXI	GCAGC	2 cut(s)	374, 1144
BsgI	GTGCAG	1 cut(s)	1066
BshFI	GGCC	3 cut(s)	703, 947, 1461
BsiHKAI	GWGCWC	1 cut(s)	806
BslFI	GGGAC	1 cut(s)	290
BslI	CCNNNNNNNGG	3 cut(s)	116, 824, 1123
BsmAI	GTCTC	1 cut(s)	1380
BsmBI	CGTCTC	1 cut(s)	1380
BsmFI	GGGAC	1 cut(s)	290
BsmI	GAATGC	2 cut(s)	15, 982
BsnI	GGCC	3 cut(s)	703, 947, 1461
Bsp1286I	GDGCHC	3 cut(s)	230, 806, 886
Bsp143I	GATC	4 cut(s)	904, 1119, 1408, 1479
Bsp19I	CCATGG	1 cut(s)	1094
BspACI	CCGC	2 cut(s)	1183, 1288
BspANI	GGCC	3 cut(s)	703, 947, 1461
BspCNI	CTCAG	2 cut(s)	1045, 1288
BspLI	GGNNCC	3 cut(s)	58, 816, 906
BspMAI	CTGCAG	2 cut(s)	292, 364
BspPI	GGATC	3 cut(s)	899, 912, 1403
BsrDI	GCAATG	2 cut(s)	184, 340
BsrI	ACTGG	5 cut(s)	58, 67, 525, 588, 932
BssECI	CCNNGG	3 cut(s)	369, 1094, 1456
BssMI	GATC	4 cut(s)	904, 1119, 1408, 1479
BssNI	GRCGYC	1 cut(s)	498
BssT1I	CCWWGG	1 cut(s)	1094
Bst2UI	CCWGG	3 cut(s)	825, 1002, 1458
Bst4CI	ACNGT	3 cut(s)	370, 796, 850
BstACI	GRCGYC	1 cut(s)	498
BstAPI	GCANNNNNTGC	1 cut(s)	632
BstC8I	GCNNGC	1 cut(s)	173
BstDEI	CTNAG	5 cut(s)	46, 98, 212, 1053, 1296
BstDSI	CCRYGG	2 cut(s)	369, 1094
BstF5I	GGATG	4 cut(s)	409, 547, 1114, 1452
BstHHI	GCGC	1 cut(s)	177
BstKTI	GATC	4 cut(s)	907, 1122, 1411, 1482
BstMAI	GTCTC	1 cut(s)	1380
BstMBI	GATC	4 cut(s)	904, 1119, 1408, 1479
BstMWI	GCNNNNNNNGC	3 cut(s)	149, 158, 632
BstNI	CCWGG	3 cut(s)	825, 1002, 1458
BstNSI	RCATGY	6 cut(s)	175, 431, 894, 1177, 1192, 1475
BstSCI	CCNGG	3 cut(s)	823, 1000, 1456
BstSFI	CTRYAG	4 cut(s)	288, 360, 792, 909
BstSLI	GKGCMC	2 cut(s)	230, 886
BstV1I	GCAGC	2 cut(s)	374, 1144
BstV2I	GAAGAC	1 cut(s)	1437
BstX2I	RGATCY	1 cut(s)	904
BstYI	RGATCY	1 cut(s)	904
BsuRI	GGCC	3 cut(s)	703, 947, 1461
BtgI	CCRYGG	2 cut(s)	369, 1094
BtgZI	GCGATG	4 cut(s)	152, 743, 980, 1298
BtsCI	GGATG	4 cut(s)	409, 547, 1114, 1452
BtsIMutI	CAGTG	2 cut(s)	60, 884
Cac8I	GCNNGC	1 cut(s)	173
CfoI	GCGC	1 cut(s)	177
CseI	GACGC	2 cut(s)	487, 1397
Csp6I	GTAC	5 cut(s)	581, 708, 867, 1171, 1446
CviAII	CATG	9 cut(s)	172, 428, 595, 891, 1016, 1095, 1174, 1189, 1472
CviQI	GTAC	5 cut(s)	581, 708, 867, 1171, 1446
DdeI	CTNAG	5 cut(s)	46, 98, 212, 1053, 1296
DpnI	GATC	4 cut(s)	906, 1121, 1410, 1481
DpnII	GATC	4 cut(s)	904, 1119, 1408, 1479
EaeI	YGGCCR	2 cut(s)	701, 945
Eco130I	CCWWGG	1 cut(s)	1094
Eco32I	GATATC	1 cut(s)	26
Eco57I	CTGAAG	1 cut(s)	621
EcoRII	CCWGG	3 cut(s)	823, 1000, 1456
EcoRV	GATATC	1 cut(s)	26
EcoT14I	CCWWGG	1 cut(s)	1094
EcoT22I	ATGCAT	1 cut(s)	1421
ErhI	CCWWGG	1 cut(s)	1094
Esp3I	CGTCTC	1 cut(s)	1380
FaeI	CATG	9 cut(s)	175, 431, 598, 894, 1019, 1098, 1177, 1192, 1475
FalI	AAGNNNNNCTT	6 cut(s)	29, 61, 729, 761, 1316, 1348
FaqI	GGGAC	1 cut(s)	290
FatI	CATG	9 cut(s)	171, 427, 594, 890, 1015, 1094, 1173, 1188, 1471
FbaI	TGATCA	1 cut(s)	1479
Fnu4HI	GCNGC	2 cut(s)	363, 1158
FokI	GGATG	4 cut(s)	416, 554, 1121, 1439
Fsp4HI	GCNGC	2 cut(s)	363, 1158
FspBI	CTAG	4 cut(s)	20, 71, 296, 461
FspI	TGCGCA	1 cut(s)	176
GlaI	GCGC	1 cut(s)	176
GluI	GCNGC	2 cut(s)	363, 1158
GsuI	CTGGAG	3 cut(s)	50, 75, 542
HaeIII	GGCC	3 cut(s)	703, 947, 1461
HgaI	GACGC	2 cut(s)	487, 1397
HhaI	GCGC	1 cut(s)	177
Hin1I	GRCGYC	1 cut(s)	498
Hin1II	CATG	9 cut(s)	175, 431, 598, 894, 1019, 1098, 1177, 1192, 1475
Hin6I	GCGC	1 cut(s)	175
HinP1I	GCGC	1 cut(s)	175
HinfI	GANTC	4 cut(s)	239, 527, 928, 1113
Hpy166II	GTNNAC	4 cut(s)	475, 720, 1059, 1146
Hpy188I	TCNGA	6 cut(s)	148, 503, 571, 640, 1054, 1467
Hpy188III	TCNNGA	6 cut(s)	29, 746, 992, 1117, 1216, 1244
Hpy8I	GTNNAC	4 cut(s)	475, 720, 1059, 1146
Hpy99I	CGWCG	1 cut(s)	503
HpyAV	CCTTC	7 cut(s)	140, 253, 498, 658, 871, 1356, 1472
HpyCH4III	ACNGT	3 cut(s)	370, 796, 850
HpyCH4IV	ACGT	3 cut(s)	279, 325, 1025
HpyCH4V	TGCA	9 cut(s)	290, 333, 362, 427, 680, 1083, 1160, 1177, 1419
HpyF10VI	GCNNNNNNNGC	3 cut(s)	149, 158, 632
HpyF3I	CTNAG	5 cut(s)	46, 98, 212, 1053, 1296
HpySE526I	ACGT	3 cut(s)	279, 325, 1025
Hsp92I	GRCGYC	1 cut(s)	498
Hsp92II	CATG	9 cut(s)	175, 431, 598, 894, 1019, 1098, 1177, 1192, 1475
HspAI	GCGC	1 cut(s)	175
Ksp22I	TGATCA	1 cut(s)	1479
Kzo9I	GATC	4 cut(s)	904, 1119, 1408, 1479
LmnI	GCTCC	1 cut(s)	56
Lsp1109I	GCAGC	2 cut(s)	374, 1144
LweI	GCATC	5 cut(s)	139, 1275, 1338, 1406, 1431
MaeI	CTAG	4 cut(s)	20, 71, 296, 461
MaeII	ACGT	3 cut(s)	279, 325, 1025
MaeIII	GTNAC	4 cut(s)	49, 796, 839, 1191
MalI	GATC	4 cut(s)	906, 1121, 1410, 1481
MboI	GATC	4 cut(s)	904, 1119, 1408, 1479
MboII	GAAGA	3 cut(s)	103, 457, 1442
MflI	RGATCY	1 cut(s)	904
MhlI	GDGCHC	3 cut(s)	230, 806, 886
MlsI	TGGCCA	2 cut(s)	703, 947
MluNI	TGGCCA	2 cut(s)	703, 947
MlyI	GAGTC	1 cut(s)	521
MmeI	TCCRAC	1 cut(s)	481
MnlI	CCTC	4 cut(s)	312, 517, 541, 1195
Mox20I	TGGCCA	2 cut(s)	703, 947
Mph1103I	ATGCAT	1 cut(s)	1421
MroXI	GAANNNNTTC	2 cut(s)	819, 1142
MscI	TGGCCA	2 cut(s)	703, 947
MseI	TTAA	5 cut(s)	378, 611, 1044, 1208, 1401
MslI	CAYNNNNRTG	3 cut(s)	599, 889, 1151
Msp20I	TGGCCA	2 cut(s)	703, 947
MspA1I	CMGCKG	1 cut(s)	914
MspR9I	CCNGG	3 cut(s)	825, 1002, 1458
Mva1269I	GAATGC	2 cut(s)	15, 982
MvaI	CCWGG	3 cut(s)	825, 1002, 1458
MwoI	GCNNNNNNNGC	3 cut(s)	149, 158, 632
NcoI	CCATGG	1 cut(s)	1094
NdeII	GATC	4 cut(s)	904, 1119, 1408, 1479
NlaIII	CATG	9 cut(s)	175, 431, 598, 894, 1019, 1098, 1177, 1192, 1475
NlaIV	GGNNCC	3 cut(s)	58, 816, 906
NsbI	TGCGCA	1 cut(s)	176
NsiI	ATGCAT	1 cut(s)	1421
NspI	RCATGY	6 cut(s)	175, 431, 894, 1177, 1192, 1475
PaeI	GCATGC	1 cut(s)	175
PciI	ACATGT	3 cut(s)	890, 1188, 1471
PctI	GAATGC	2 cut(s)	15, 982
PdmI	GAANNNNTTC	2 cut(s)	819, 1142
PfeI	GAWTC	3 cut(s)	239, 928, 1113
PflMI	CCANNNNNTGG	1 cut(s)	116
PfoI	TCCNGGA	1 cut(s)	1000
PkrI	GCNGC	2 cut(s)	364, 1159
PleI	GAGTC	1 cut(s)	521
PpsI	GAGTC	1 cut(s)	521
PscI	ACATGT	3 cut(s)	890, 1188, 1471
PsiI	TTATAA	2 cut(s)	137, 1104
Psp6I	CCWGG	3 cut(s)	823, 1000, 1456
PspGI	CCWGG	3 cut(s)	823, 1000, 1456
PspN4I	GGNNCC	3 cut(s)	58, 816, 906
PstI	CTGCAG	2 cut(s)	292, 364
PsuI	RGATCY	1 cut(s)	904
PvuII	CAGCTG	1 cut(s)	914
RsaI	GTAC	5 cut(s)	582, 709, 868, 1172, 1447
RsaNI	GTAC	5 cut(s)	581, 708, 867, 1171, 1446
RseI	CAYNNNNRTG	3 cut(s)	599, 889, 1151
SaqAI	TTAA	5 cut(s)	378, 611, 1044, 1208, 1401
SatI	GCNGC	2 cut(s)	363, 1158
Sau3AI	GATC	4 cut(s)	904, 1119, 1408, 1479
ScaI	AGTACT	2 cut(s)	582, 709
SchI	GAGTC	1 cut(s)	521
ScrFI	CCNGG	3 cut(s)	825, 1002, 1458
SduI	GDGCHC	3 cut(s)	230, 806, 886
SfaNI	GCATC	5 cut(s)	139, 1275, 1338, 1406, 1431
SfcI	CTRYAG	4 cut(s)	288, 360, 792, 909
SmiMI	CAYNNNNRTG	3 cut(s)	599, 889, 1151
SmlI	CTYRAG	1 cut(s)	1030
SmoI	CTYRAG	1 cut(s)	1030
SphI	GCATGC	1 cut(s)	175
SsiI	CCGC	2 cut(s)	1183, 1288
SspI	AATATT	2 cut(s)	115, 1238
SspMI	CTAG	4 cut(s)	20, 71, 296, 461
StyD4I	CCNGG	3 cut(s)	823, 1000, 1456
StyI	CCWWGG	1 cut(s)	1094
TaaI	ACNGT	3 cut(s)	370, 796, 850
TaiI	ACGT	3 cut(s)	282, 328, 1028
TatI	WGTACW	2 cut(s)	580, 707
TfiI	GAWTC	3 cut(s)	239, 928, 1113
Tru1I	TTAA	5 cut(s)	378, 611, 1044, 1208, 1401
Tru9I	TTAA	5 cut(s)	378, 611, 1044, 1208, 1401
TscAI	CASTG	2 cut(s)	67, 891
TseI	GCWGC	2 cut(s)	362, 1157
TspDTI	ATGAA	5 cut(s)	235, 344, 351, 630, 677
TspRI	CASTG	2 cut(s)	67, 891
Van91I	CCANNNNNTGG	1 cut(s)	116
XapI	RAATTY	3 cut(s)	1063, 1226, 1395
XceI	RCATGY	6 cut(s)	175, 431, 894, 1177, 1192, 1475
XcmI	CCANNNNNNNNNTGG	1 cut(s)	825
XmnI	GAANNNNTTC	2 cut(s)	819, 1142
XspI	CTAG	4 cut(s)	20, 71, 296, 461
ZrmI	AGTACT	2 cut(s)	582, 709
Zsp2I	ATGCAT	1 cut(s)	1421

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.