Rh4AG059200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0002552
Physical Location & Seq
Forward (+)
12444146 .. 12446284
2139 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG059200.1

Sequence Viewer

Length: 270 bp
ATGGCGCCGAGGCTCGGCTCCGGCGACGAATCCAGCTTCAAGTTCCTTCTCTTTCTGGTCGAGAATTACCACCTCATCGCCGTGCTTGTCAACTTTGGTGAGAGAAATGAATGGCGGTGGAGAAGGGCTCGCGGCTTCGGCGACGTCTGGAGCGGCGATGGCGCCACTCGACTGGATGTTTCTCAAGTTTCTGGAGAACTGACCGCCGGCGAGGAAGTTCAGGAAGGATACCCAATAGGAGCTATGATGACACGACAGCAAGTTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

89

Amino Acids

10.03

Weight (kDa)

5.26

Isoelectric Point (pI)

32.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 147
AccB1I GGYRCC 2 cut(s) 4, 161
AccBSI CCGCTC 1 cut(s) 153
AccII CGCG 1 cut(s) 132
AciI CCGC 4 cut(s) 115, 132, 153, 204
AcyI GRCGYC 3 cut(s) 5, 144, 162
AfiI CCNNNNNNNGG 1 cut(s) 14
AgsI TTSAA 1 cut(s) 40
AluBI AGCT 2 cut(s) 36, 242
AluI AGCT 2 cut(s) 36, 242
AspLEI GCGC 2 cut(s) 7, 164
AsuHPI GGTGA 1 cut(s) 110
BanI GGYRCC 2 cut(s) 4, 161
BanII GRGCYC 1 cut(s) 130
BccI CCATC 1 cut(s) 152
BceAI ACGGC 1 cut(s) 65
BciVI GTATCC 1 cut(s) 221
BfoI RGCGCY 2 cut(s) 8, 165
BfuI GTATCC 1 cut(s) 221
BisI GCNGC 2 cut(s) 133, 154
BlsI GCNGC 2 cut(s) 134, 155
BmiI GGNNCC 3 cut(s) 6, 19, 163
BplI GAGNNNNNCTC 2 cut(s) 112, 144
BpmI CTGGAG 2 cut(s) 169, 213
BpuEI CTTGAG 1 cut(s) 168
BsaHI GRCGYC 3 cut(s) 5, 144, 162
BsaJI CCNNGG 1 cut(s) 8
Bsc4I CCNNNNNNNGG 1 cut(s) 14
Bse118I RCCGGY 1 cut(s) 206
Bse1I ACTGG 1 cut(s) 177
BseDI CCNNGG 1 cut(s) 8
BseGI GGATG 1 cut(s) 181
BseLI CCNNNNNNNGG 1 cut(s) 14
BseNI ACTGG 1 cut(s) 177
Bsh1236I CGCG 1 cut(s) 132
BshNI GGYRCC 2 cut(s) 4, 161
BsiSI CCGG 2 cut(s) 21, 207
BslI CCNNNNNNNGG 1 cut(s) 14
Bsp1286I GDGCHC 1 cut(s) 130
BspACI CCGC 4 cut(s) 115, 132, 153, 204
BspFNI CGCG 1 cut(s) 132
BspLI GGNNCC 3 cut(s) 6, 19, 163
BspT107I GGYRCC 2 cut(s) 4, 161
BsrBI CCGCTC 1 cut(s) 153
BsrFI RCCGGY 1 cut(s) 206
BsrI ACTGG 1 cut(s) 177
BssAI RCCGGY 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 8
BssNI GRCGYC 3 cut(s) 5, 144, 162
BstACI GRCGYC 3 cut(s) 5, 144, 162
BstC8I GCNNGC 2 cut(s) 130, 208
BstF5I GGATG 1 cut(s) 181
BstFNI CGCG 1 cut(s) 132
BstH2I RGCGCY 2 cut(s) 8, 165
BstHHI GCGC 2 cut(s) 7, 164
BstMWI GCNNNNNNNGC 2 cut(s) 138, 159
BstUI CGCG 1 cut(s) 132
BstXI CCANNNNNNTGG 1 cut(s) 172
BsuI GTATCC 1 cut(s) 221
BtgZI GCGATG 2 cut(s) 61, 171
BtsCI GGATG 1 cut(s) 181
Cac8I GCNNGC 2 cut(s) 130, 208
CfoI GCGC 2 cut(s) 7, 164
Cfr10I RCCGGY 1 cut(s) 206
CviJI RGCY 6 cut(s) 13, 18, 36, 128, 135, 242
CviKI_1 RGCY 6 cut(s) 13, 18, 36, 128, 135, 242
DinI GGCGCC 2 cut(s) 6, 163
Eco24I GRGCYC 1 cut(s) 130
EcoT38I GRGCYC 1 cut(s) 130
EgeI GGCGCC 2 cut(s) 6, 163
EheI GGCGCC 2 cut(s) 6, 163
FaiI YATR 1 cut(s) 245
Fnu4HI GCNGC 2 cut(s) 133, 154
FokI GGATG 1 cut(s) 188
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 2 cut(s) 133, 154
GlaI GCGC 2 cut(s) 6, 163
GluI GCNGC 2 cut(s) 133, 154
GsuI CTGGAG 2 cut(s) 169, 213
HaeII RGCGCY 2 cut(s) 8, 165
HapII CCGG 2 cut(s) 21, 207
HhaI GCGC 2 cut(s) 7, 164
Hin1I GRCGYC 3 cut(s) 5, 144, 162
Hin6I GCGC 2 cut(s) 5, 162
HinP1I GCGC 2 cut(s) 5, 162
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 1 cut(s) 29
HpaII CCGG 2 cut(s) 21, 207
HphI GGTGA 1 cut(s) 110
Hpy166II GTNNAC 1 cut(s) 91
Hpy188III TCNNGA 4 cut(s) 61, 148, 192, 221
Hpy8I GTNNAC 1 cut(s) 91
Hpy99I CGWCG 2 cut(s) 29, 146
HpyAV CCTTC 3 cut(s) 56, 117, 218
HpyCH4IV ACGT 1 cut(s) 144
HpyF10VI GCNNNNNNNGC 2 cut(s) 138, 159
HpySE526I ACGT 1 cut(s) 144
Hsp92I GRCGYC 3 cut(s) 5, 144, 162
HspAI GCGC 2 cut(s) 5, 162
KasI GGCGCC 2 cut(s) 4, 161
KroI GCCGGC 1 cut(s) 206
KroNI GCCGGC 1 cut(s) 208
LmnI GCTCC 3 cut(s) 23, 150, 239
LpnPI CCDG 8 cut(s) 34, 41, 46, 133, 158, 177, 206, 220
MaeII ACGT 1 cut(s) 144
MbiI CCGCTC 1 cut(s) 153
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 1 cut(s) 64
Mly113I GGCGCC 2 cut(s) 5, 162
MnlI CCTC 3 cut(s) 3, 83, 205
MreI CGCCGGCG 1 cut(s) 206
MroNI GCCGGC 1 cut(s) 206
MseI TTAA 1 cut(s) 268
MslI CAYNNNNRTG 1 cut(s) 80
MspI CCGG 2 cut(s) 21, 207
MvnI CGCG 1 cut(s) 132
MwoI GCNNNNNNNGC 2 cut(s) 138, 159
NaeI GCCGGC 1 cut(s) 208
NarI GGCGCC 2 cut(s) 5, 162
NgoMIV GCCGGC 1 cut(s) 206
NlaIV GGNNCC 3 cut(s) 6, 19, 163
NmeAIII GCCGAG 1 cut(s) 33
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PdiI GCCGGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 29
PkrI GCNGC 2 cut(s) 134, 155
PluTI GGCGCC 2 cut(s) 8, 165
PspN4I GGNNCC 3 cut(s) 6, 19, 163
RseI CAYNNNNRTG 1 cut(s) 80
SaqAI TTAA 1 cut(s) 268
SatI GCNGC 2 cut(s) 133, 154
SduI GDGCHC 1 cut(s) 130
SetI ASST 4 cut(s) 38, 75, 147, 244
SfoI GGCGCC 2 cut(s) 6, 163
SgrAI CRCCGGYG 1 cut(s) 206
SmiMI CAYNNNNRTG 1 cut(s) 80
SmlI CTYRAG 1 cut(s) 183
SmoI CTYRAG 1 cut(s) 183
Sse9I AATT 1 cut(s) 64
SsiI CCGC 4 cut(s) 115, 132, 153, 204
SspDI GGCGCC 2 cut(s) 4, 161
TaiI ACGT 1 cut(s) 147
TaqI TCGA 2 cut(s) 60, 169
TasI AATT 1 cut(s) 64
TauI GCSGC 2 cut(s) 135, 156
TfiI GAWTC 1 cut(s) 29
Tru1I TTAA 1 cut(s) 268
Tru9I TTAA 1 cut(s) 268
TspDTI ATGAA 2 cut(s) 123, 254
ZraI GACGTC 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.