Rh5AG065800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001940
Physical Location & Seq
Reverse (-)
5515546 .. 5518539
2994 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG065800.1

Sequence Viewer

Length: 477 bp
ATGGCTACTGATGGGAATGCTATTGCACCATCGCCGACAGCTCCAGTGGAACCAAATCTTGCAACACCTGATGCTCCGGTTAGGCCTACAGCTTCGACATCTGGTCGTCCTCCAAGACCAACAAAAGCCAAGAGGAAGAGGGCGGAGAAGAAGGCAGTCCAGTCACTGAGTGTCACAACCAGGTCCGACGTCTGGGCACACTTTGAGAAGTTTGATCGGCCAATAGTGGAAGTGGTAGATGGAAAGAAGCAGGAAGTTGGACGTCAAATTATGCGAAGGAGAAATCCGGTGAACAATCAGCAAAAAGGGCTGCTTGTAAGGCAAAAAAATCAAAGCAGGCTGAGAATTCAGATTGTTCTTGACTTCTTCTATTTGAATTTGGATCTTGAACTCTACATATTTGGATATTTGAATGTGCAGGGTGAACTTTGGATATGGATATTGGATAGTGTTTTGAACTTCAACATGTTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

18.04

Weight (kDa)

10.22

Isoelectric Point (pI)

52.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 192, 265
AciI CCGC 1 cut(s) 143
AclWI GGATC 1 cut(s) 390
AcoI YGGCCR 1 cut(s) 218
AcsI RAATTY 2 cut(s) 345, 376
AcyI GRCGYC 2 cut(s) 189, 262
AdeI CACNNNGTG 1 cut(s) 170
AfiI CCNNNNNNNGG 1 cut(s) 192
AflIII ACRYGT 1 cut(s) 465
AgsI TTSAA 6 cut(s) 376, 389, 412, 457, 463, 472
AhdI GACNNNNNGTC 1 cut(s) 102
AjnI CCWGG 1 cut(s) 179
AluBI AGCT 2 cut(s) 41, 92
AluI AGCT 2 cut(s) 41, 92
AlwI GGATC 1 cut(s) 390
AlwNI CAGNNNCTG 1 cut(s) 166
AoxI GGCC 2 cut(s) 83, 218
ApeKI GCWGC 1 cut(s) 310
ApoI RAATTY 2 cut(s) 345, 376
AspS9I GGNCC 1 cut(s) 183
AsuHPI GGTGA 2 cut(s) 301, 434
AvaII GGWCC 1 cut(s) 183
BaeGI GKGCMC 1 cut(s) 199
BbvI GCAGC 1 cut(s) 297
BccI CCATC 3 cut(s) 5, 37, 233
BciT130I CCWGG 1 cut(s) 181
BfmI CTRYAG 1 cut(s) 87
BisI GCNGC 1 cut(s) 311
BlsI GCNGC 1 cut(s) 312
Bme1390I CCNGG 1 cut(s) 181
Bme18I GGWCC 1 cut(s) 183
BmeRI GACNNNNNGTC 1 cut(s) 102
BmgT120I GGNCC 1 cut(s) 183
BmiI GGNNCC 1 cut(s) 51
BmrFI CCNGG 1 cut(s) 181
BmsI GCATC 1 cut(s) 61
BpmI CTGGAG 1 cut(s) 27
BsaHI GRCGYC 2 cut(s) 189, 262
BsaWI WCCGGW 2 cut(s) 76, 286
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse1I ACTGG 2 cut(s) 44, 160
BseBI CCWGG 1 cut(s) 181
BseLI CCNNNNNNNGG 1 cut(s) 192
BseMII CTCAG 2 cut(s) 158, 332
BseNI ACTGG 2 cut(s) 44, 160
BseSI GKGCMC 1 cut(s) 199
BseXI GCAGC 1 cut(s) 297
BsgI GTGCAG 1 cut(s) 437
BshFI GGCC 2 cut(s) 85, 220
BsiSI CCGG 2 cut(s) 77, 287
BslI CCNNNNNNNGG 1 cut(s) 192
BsmI GAATGC 1 cut(s) 22
BsnI GGCC 2 cut(s) 85, 220
Bsp1286I GDGCHC 1 cut(s) 199
Bsp143I GATC 2 cut(s) 214, 382
BspACI CCGC 1 cut(s) 143
BspANI GGCC 2 cut(s) 85, 220
BspCNI CTCAG 2 cut(s) 159, 333
BspLI GGNNCC 1 cut(s) 51
BspPI GGATC 1 cut(s) 390
BsrI ACTGG 2 cut(s) 44, 160
BssMI GATC 2 cut(s) 214, 382
BssNI GRCGYC 2 cut(s) 189, 262
Bst2UI CCWGG 1 cut(s) 181
Bst6I CTCTTC 1 cut(s) 131
BstACI GRCGYC 2 cut(s) 189, 262
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 2 cut(s) 167, 341
BstKTI GATC 2 cut(s) 217, 385
BstMBI GATC 2 cut(s) 214, 382
BstMWI GCNNNNNNNGC 2 cut(s) 307, 319
BstNI CCWGG 1 cut(s) 181
BstNSI RCATGY 1 cut(s) 469
BstSCI CCNGG 1 cut(s) 179
BstSFI CTRYAG 1 cut(s) 87
BstSLI GKGCMC 1 cut(s) 199
BstV1I GCAGC 1 cut(s) 297
BstX2I RGATCY 1 cut(s) 382
BstYI RGATCY 1 cut(s) 382
BsuRI GGCC 2 cut(s) 85, 220
BtgZI GCGATG 1 cut(s) 15
BtsIMutI CAGTG 2 cut(s) 51, 164
Cac8I GCNNGC 1 cut(s) 338
CaiI CAGNNNCTG 1 cut(s) 166
Cfr13I GGNCC 1 cut(s) 183
CsiI ACCWGGT 1 cut(s) 179
CviAII CATG 1 cut(s) 466
CviJI RGCY 8 cut(s) 5, 41, 85, 92, 128, 220, 310, 340
CviKI_1 RGCY 8 cut(s) 5, 41, 85, 92, 128, 220, 310, 340
DdeI CTNAG 2 cut(s) 167, 341
DpnI GATC 2 cut(s) 216, 384
DpnII GATC 2 cut(s) 214, 382
DraIII CACNNNGTG 1 cut(s) 170
DriI GACNNNNNGTC 1 cut(s) 102
EaeI YGGCCR 1 cut(s) 218
Eam1104I CTCTTC 1 cut(s) 131
Eam1105I GACNNNNNGTC 1 cut(s) 102
EarI CTCTTC 1 cut(s) 131
EciI GGCGGA 1 cut(s) 158
Eco147I AGGCCT 1 cut(s) 85
Eco47I GGWCC 1 cut(s) 183
EcoRI GAATTC 1 cut(s) 345
EcoRII CCWGG 1 cut(s) 179
FaeI CATG 1 cut(s) 469
FaiI YATR 4 cut(s) 272, 398, 436, 467
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FatI CATG 1 cut(s) 465
Fnu4HI GCNGC 1 cut(s) 311
Fsp4HI GCNGC 1 cut(s) 311
GluI GCNGC 1 cut(s) 311
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 2 cut(s) 85, 220
HapII CCGG 2 cut(s) 77, 287
Hin1I GRCGYC 2 cut(s) 189, 262
Hin1II CATG 1 cut(s) 469
HpaII CCGG 2 cut(s) 77, 287
HphI GGTGA 2 cut(s) 301, 434
Hpy166II GTNNAC 2 cut(s) 292, 425
Hpy188I TCNGA 2 cut(s) 187, 351
Hpy188III TCNNGA 2 cut(s) 359, 386
Hpy8I GTNNAC 2 cut(s) 292, 425
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 2 cut(s) 145, 270
HpyCH4IV ACGT 2 cut(s) 189, 262
HpyCH4V TGCA 3 cut(s) 26, 62, 418
HpyF10VI GCNNNNNNNGC 2 cut(s) 307, 319
HpyF3I CTNAG 2 cut(s) 167, 341
HpySE526I ACGT 2 cut(s) 189, 262
Hsp92I GRCGYC 2 cut(s) 189, 262
Hsp92II CATG 1 cut(s) 469
Kzo9I GATC 2 cut(s) 214, 382
LmnI GCTCC 2 cut(s) 46, 79
Lsp1109I GCAGC 1 cut(s) 297
LweI GCATC 1 cut(s) 61
MabI ACCWGGT 1 cut(s) 179
MaeII ACGT 2 cut(s) 189, 262
MaeIII GTNAC 2 cut(s) 162, 172
MalI GATC 2 cut(s) 216, 384
MboI GATC 2 cut(s) 214, 382
MboII GAAGA 3 cut(s) 148, 160, 358
MflI RGATCY 1 cut(s) 382
MhlI GDGCHC 1 cut(s) 199
MluCI AATT 3 cut(s) 267, 345, 376
MmeI TCCRAC 2 cut(s) 210, 238
MnlI CCTC 3 cut(s) 120, 126, 132
MspI CCGG 2 cut(s) 77, 287
MspR9I CCNGG 1 cut(s) 181
Mva1269I GAATGC 1 cut(s) 22
MvaI CCWGG 1 cut(s) 181
MwoI GCNNNNNNNGC 2 cut(s) 307, 319
NdeII GATC 2 cut(s) 214, 382
NlaIII CATG 1 cut(s) 469
NlaIV GGNNCC 1 cut(s) 51
NmuCI GTSAC 2 cut(s) 162, 172
NspI RCATGY 1 cut(s) 469
PceI AGGCCT 1 cut(s) 85
PciI ACATGT 1 cut(s) 465
PctI GAATGC 1 cut(s) 22
PkrI GCNGC 1 cut(s) 312
PscI ACATGT 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 179
PspGI CCWGG 1 cut(s) 179
PspN4I GGNNCC 1 cut(s) 51
PspPI GGNCC 1 cut(s) 183
PstNI CAGNNNCTG 1 cut(s) 166
PsuI RGATCY 1 cut(s) 382
SatI GCNGC 1 cut(s) 311
Sau3AI GATC 2 cut(s) 214, 382
Sau96I GGNCC 1 cut(s) 183
ScrFI CCNGG 1 cut(s) 181
SduI GDGCHC 1 cut(s) 199
SetI ASST 6 cut(s) 43, 70, 94, 185, 192, 265
SexAI ACCWGGT 1 cut(s) 179
SfaNI GCATC 1 cut(s) 61
SfcI CTRYAG 1 cut(s) 87
SinI GGWCC 1 cut(s) 183
Sse9I AATT 3 cut(s) 267, 345, 376
SseBI AGGCCT 1 cut(s) 85
SsiI CCGC 1 cut(s) 143
StuI AGGCCT 1 cut(s) 85
StyD4I CCNGG 1 cut(s) 179
TaiI ACGT 2 cut(s) 192, 265
TaqI TCGA 1 cut(s) 95
TasI AATT 3 cut(s) 267, 345, 376
TscAI CASTG 2 cut(s) 51, 171
TseFI GTSAC 2 cut(s) 162, 172
TseI GCWGC 1 cut(s) 310
Tsp45I GTSAC 2 cut(s) 162, 172
TspRI CASTG 2 cut(s) 51, 171
VpaK11BI GGWCC 1 cut(s) 183
XapI RAATTY 2 cut(s) 345, 376
XceI RCATGY 1 cut(s) 469
ZraI GACGTC 2 cut(s) 190, 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.