Rh5AG325400

Cgr1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0001180
Physical Location & Seq
Forward (+)
48834417 .. 48835945
1529 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG325400.1

Sequence Viewer

Length: 591 bp
ATGGCCTGCACAATCGACTTCCGCTGCCTAGACGAAGGCTTCGGCGGCAAAACCTACAAGCGCAAGCGGAACCCGGAAGCCGCCGCCGACGACGAAGATGCCGCCATGGAAATCGACGCCGCGGCCTATCCACCTCCGGCGAAGCGATCCGCGGTGTCCTCCTCGGACAATCCGGACAAACCGGTCTTCGGCCGGCCGAGCTACGACGGCGTGATCGCCGGGAAGGTGTCCGGGCGGAAGTGGAAGCAGCCGCGGAAGCAGAGGTCGTCGGCGCTCCAGGTGAGCCGGAAGGGGACGACGTTCGAGGAGAGGGAGAAGAACAAGTCGGTGAAGAAGGCGTACAAGGAGAGAATGGCGGAGCTGAAGGGGGAGATTAAGAGGAACAAGGAGGAGAAGAGGAGGAAGAGGGAGGAGAGGGAGAAGAAGAAGAAGGAGAATATTCTCAAGACTGGGACCAAGCTGCAGATTATTTCCAACCCCAAGACGATTCAGAGGATTTCGAAAACCAAGGCCAGGAATCAACTCAAGCCTGTTCCCGAGGAGATGATCAAGAGGAACAATAAGAAGAGCGGCCTTTTGCTTACGGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cgr1

Protein Analysis

196

Amino Acids

22.38

Weight (kDa)

10.11

Isoelectric Point (pI)

67.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cgr1 PF03879 74 - 174 2.8e-07 Cgr1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 182
AccBSI CCGCTC 1 cut(s) 570
AccII CGCG 3 cut(s) 122, 152, 253
AccIII TCCGGA 1 cut(s) 172
AclWI GGATC 1 cut(s) 141
AcoI YGGCCR 2 cut(s) 190, 194
AcuI CTGAAG 1 cut(s) 383
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 1 cut(s) 341
AfiI CCNNNNNNNGG 2 cut(s) 188, 513
AgeI ACCGGT 1 cut(s) 181
AjnI CCWGG 2 cut(s) 276, 512
AluBI AGCT 3 cut(s) 201, 361, 460
AluI AGCT 3 cut(s) 201, 361, 460
AlwI GGATC 1 cut(s) 141
Ama87I CYCGRG 1 cut(s) 536
Aor13HI TCCGGA 1 cut(s) 172
AoxI GGCC 6 cut(s) 3, 123, 190, 194, 510, 571
ApeKI GCWGC 3 cut(s) 24, 247, 460
ArsI GACNNNNNNTTYG 2 cut(s) 23, 55
AsiGI ACCGGT 1 cut(s) 181
AspLEI GCGC 2 cut(s) 63, 274
AspS9I GGNCC 1 cut(s) 453
AsuC2I CCSGG 3 cut(s) 74, 220, 232
AsuHPI GGTGA 2 cut(s) 292, 340
AsuII TTCGAA 1 cut(s) 500
AvaI CYCGRG 1 cut(s) 536
AvaII GGWCC 1 cut(s) 453
BarI GAAGNNNNNNTAC 2 cut(s) 323, 355
BbsI GAAGAC 1 cut(s) 178
BbvI GCAGC 3 cut(s) 11, 259, 447
BceAI ACGGC 1 cut(s) 223
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BciT130I CCWGG 2 cut(s) 278, 514
BclI TGATCA 1 cut(s) 546
BcnI CCSGG 3 cut(s) 74, 220, 232
BfaI CTAG 1 cut(s) 29
BfmI CTRYAG 1 cut(s) 461
BfoI RGCGCY 1 cut(s) 275
Bme1390I CCNGG 5 cut(s) 74, 220, 232, 278, 514
Bme18I GGWCC 1 cut(s) 453
BmeT110I CYCGRG 1 cut(s) 536
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 2 cut(s) 71, 454
BmrFI CCNGG 5 cut(s) 74, 220, 232, 278, 514
BmrI ACTGGG 1 cut(s) 459
BmsI GCATC 1 cut(s) 88
BmuI ACTGGG 1 cut(s) 459
BpiI GAAGAC 1 cut(s) 178
BpmI CTGGAG 1 cut(s) 260
Bpu14I TTCGAA 1 cut(s) 500
BpuEI CTTGAG 2 cut(s) 428, 509
BpuMI CCSGG 3 cut(s) 74, 220, 232
BsaHI GRCGYC 1 cut(s) 117
BsaJI CCNNGG 7 cut(s) 105, 120, 150, 162, 251, 507, 537
BsaWI WCCGGW 2 cut(s) 172, 181
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 513
Bse118I RCCGGY 2 cut(s) 181, 192
Bse1I ACTGG 1 cut(s) 454
BseAI TCCGGA 1 cut(s) 172
BseBI CCWGG 2 cut(s) 278, 514
BseDI CCNNGG 7 cut(s) 105, 120, 150, 162, 251, 507, 537
BseLI CCNNNNNNNGG 2 cut(s) 188, 513
BseNI ACTGG 1 cut(s) 454
BseRI GAGGAG 6 cut(s) 151, 320, 404, 412, 425, 554
BseX3I CGGCCG 2 cut(s) 190, 194
BseXI GCAGC 3 cut(s) 11, 259, 447
Bsh1236I CGCG 3 cut(s) 122, 152, 253
Bsh1285I CGRYCG 2 cut(s) 193, 197
BshFI GGCC 6 cut(s) 5, 125, 192, 196, 512, 573
BshTI ACCGGT 1 cut(s) 181
BsiEI CGRYCG 2 cut(s) 193, 197
BsiHKCI CYCGRG 1 cut(s) 536
BsiSI CCGG 8 cut(s) 74, 137, 173, 182, 193, 219, 231, 286
BslFI GGGAC 2 cut(s) 307, 466
BslI CCNNNNNNNGG 2 cut(s) 188, 513
BsmFI GGGAC 2 cut(s) 307, 466
BsnI GGCC 6 cut(s) 5, 125, 192, 196, 512, 573
BsoBI CYCGRG 1 cut(s) 536
Bsp119I TTCGAA 1 cut(s) 500
Bsp13I TCCGGA 1 cut(s) 172
Bsp143I GATC 3 cut(s) 146, 213, 546
Bsp19I CCATGG 1 cut(s) 105
BspANI GGCC 6 cut(s) 5, 125, 192, 196, 512, 573
BspEI TCCGGA 1 cut(s) 172
BspFNI CGCG 3 cut(s) 122, 152, 253
BspLI GGNNCC 2 cut(s) 71, 454
BspMAI CTGCAG 1 cut(s) 465
BspPI GGATC 1 cut(s) 141
BspQI GCTCTTC 1 cut(s) 560
BspT104I TTCGAA 1 cut(s) 500
BsrBI CCGCTC 1 cut(s) 570
BsrFI RCCGGY 2 cut(s) 181, 192
BsrI ACTGG 1 cut(s) 454
BssAI RCCGGY 2 cut(s) 181, 192
BssECI CCNNGG 7 cut(s) 105, 120, 150, 162, 251, 507, 537
BssMI GATC 3 cut(s) 146, 213, 546
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 2 cut(s) 105, 507
Bst2UI CCWGG 2 cut(s) 278, 514
Bst6I CTCTTC 3 cut(s) 389, 398, 560
BstACI GRCGYC 1 cut(s) 117
BstBI TTCGAA 1 cut(s) 500
BstC8I GCNNGC 3 cut(s) 7, 65, 194
BstDSI CCRYGG 4 cut(s) 105, 120, 150, 251
BstFNI CGCG 3 cut(s) 122, 152, 253
BstH2I RGCGCY 1 cut(s) 275
BstHHI GCGC 2 cut(s) 63, 274
BstKTI GATC 3 cut(s) 149, 216, 549
BstMBI GATC 3 cut(s) 146, 213, 546
BstMCI CGRYCG 2 cut(s) 193, 197
BstMWI GCNNNNNNNGC 4 cut(s) 45, 198, 207, 256
BstNI CCWGG 2 cut(s) 278, 514
BstSCI CCNGG 5 cut(s) 72, 218, 230, 276, 512
BstSFI CTRYAG 1 cut(s) 461
BstUI CGCG 3 cut(s) 122, 152, 253
BstV1I GCAGC 3 cut(s) 11, 259, 447
BstV2I GAAGAC 1 cut(s) 178
BstZI CGGCCG 2 cut(s) 190, 194
BsuRI GGCC 6 cut(s) 5, 125, 192, 196, 512, 573
BtgI CCRYGG 4 cut(s) 105, 120, 150, 251
Cac8I GCNNGC 3 cut(s) 7, 65, 194
CfoI GCGC 2 cut(s) 63, 274
Cfr10I RCCGGY 2 cut(s) 181, 192
Cfr13I GGNCC 1 cut(s) 453
Cfr42I CCGCGG 3 cut(s) 123, 153, 254
CseI GACGC 1 cut(s) 125
Csp6I GTAC 1 cut(s) 340
CspAI ACCGGT 1 cut(s) 181
CviAII CATG 1 cut(s) 106
CviQI GTAC 1 cut(s) 340
DpnI GATC 3 cut(s) 148, 215, 548
DpnII GATC 3 cut(s) 146, 213, 546
DrdI GACNNNNNNGTC 1 cut(s) 182
DseDI GACNNNNNNGTC 1 cut(s) 182
EaeI YGGCCR 2 cut(s) 190, 194
EagI CGGCCG 2 cut(s) 190, 194
Eam1104I CTCTTC 3 cut(s) 389, 398, 560
EarI CTCTTC 3 cut(s) 389, 398, 560
EciI GGCGGA 2 cut(s) 250, 371
EclXI CGGCCG 2 cut(s) 190, 194
Eco130I CCWWGG 2 cut(s) 105, 507
Eco47I GGWCC 1 cut(s) 453
Eco52I CGGCCG 2 cut(s) 190, 194
Eco57I CTGAAG 1 cut(s) 383
Eco88I CYCGRG 1 cut(s) 536
EcoRII CCWGG 2 cut(s) 276, 512
EcoT14I CCWWGG 2 cut(s) 105, 507
ErhI CCWWGG 2 cut(s) 105, 507
FaeI CATG 1 cut(s) 109
FaiI YATR 1 cut(s) 107
FaqI GGGAC 2 cut(s) 307, 466
FatI CATG 1 cut(s) 105
FbaI TGATCA 1 cut(s) 546
FseI GGCCGGCC 1 cut(s) 196
FspBI CTAG 1 cut(s) 29
GlaI GCGC 2 cut(s) 62, 273
GsuI CTGGAG 1 cut(s) 260
HaeII RGCGCY 1 cut(s) 275
HaeIII GGCC 6 cut(s) 5, 125, 192, 196, 512, 573
HapII CCGG 8 cut(s) 74, 137, 173, 182, 193, 219, 231, 286
HgaI GACGC 1 cut(s) 125
HhaI GCGC 2 cut(s) 63, 274
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 1 cut(s) 109
Hin6I GCGC 2 cut(s) 61, 272
HinP1I GCGC 2 cut(s) 61, 272
HinfI GANTC 2 cut(s) 487, 517
HpaII CCGG 8 cut(s) 74, 137, 173, 182, 193, 219, 231, 286
HphI GGTGA 2 cut(s) 292, 340
Hpy188I TCNGA 2 cut(s) 166, 492
Hpy188III TCNNGA 4 cut(s) 173, 445, 536, 550
Hpy99I CGWCG 6 cut(s) 92, 95, 119, 209, 271, 301
HpyAV CCTTC 6 cut(s) 29, 217, 283, 328, 358, 424
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 2 cut(s) 9, 463
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 198, 207, 256
HpySE526I ACGT 1 cut(s) 299
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 1 cut(s) 109
HspAI GCGC 2 cut(s) 61, 272
Kpn2I TCCGGA 1 cut(s) 172
KroI GCCGGC 1 cut(s) 192
KroNI GCCGGC 1 cut(s) 194
Ksp22I TGATCA 1 cut(s) 546
KspI CCGCGG 3 cut(s) 123, 153, 254
Kzo9I GATC 3 cut(s) 146, 213, 546
LguI GCTCTTC 1 cut(s) 560
LmnI GCTCC 2 cut(s) 279, 358
Lsp1109I GCAGC 3 cut(s) 11, 259, 447
LweI GCATC 1 cut(s) 88
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 299
MalI GATC 3 cut(s) 148, 215, 548
MbiI CCGCTC 1 cut(s) 570
MboI GATC 3 cut(s) 146, 213, 546
MmeI TCCRAC 1 cut(s) 498
MroI TCCGGA 1 cut(s) 172
MroNI GCCGGC 1 cut(s) 192
MseI TTAA 1 cut(s) 375
MspA1I CMGCKG 4 cut(s) 24, 122, 152, 253
MspI CCGG 8 cut(s) 74, 137, 173, 182, 193, 219, 231, 286
MspR9I CCNGG 5 cut(s) 74, 220, 232, 278, 514
MvaI CCWGG 2 cut(s) 278, 514
MvnI CGCG 3 cut(s) 122, 152, 253
MwoI GCNNNNNNNGC 4 cut(s) 45, 198, 207, 256
NaeI GCCGGC 1 cut(s) 194
NciI CCSGG 3 cut(s) 74, 220, 232
NcoI CCATGG 1 cut(s) 105
NdeII GATC 3 cut(s) 146, 213, 546
NgoMIV GCCGGC 1 cut(s) 192
NlaIII CATG 1 cut(s) 109
NlaIV GGNNCC 2 cut(s) 71, 454
NmeAIII GCCGAG 1 cut(s) 222
NspV TTCGAA 1 cut(s) 500
PciSI GCTCTTC 1 cut(s) 560
PdiI GCCGGC 1 cut(s) 194
PfeI GAWTC 2 cut(s) 487, 517
PinAI ACCGGT 1 cut(s) 181
Psp6I CCWGG 2 cut(s) 276, 512
PspGI CCWGG 2 cut(s) 276, 512
PspN4I GGNNCC 2 cut(s) 71, 454
PspPI GGNCC 1 cut(s) 453
PstI CTGCAG 1 cut(s) 465
RigI GGCCGGCC 1 cut(s) 196
RsaI GTAC 1 cut(s) 341
RsaNI GTAC 1 cut(s) 340
SacII CCGCGG 3 cut(s) 123, 153, 254
SapI GCTCTTC 1 cut(s) 560
SaqAI TTAA 1 cut(s) 375
Sau3AI GATC 3 cut(s) 146, 213, 546
Sau96I GGNCC 1 cut(s) 453
ScrFI CCNGG 5 cut(s) 74, 220, 232, 278, 514
SetI ASST 9 cut(s) 56, 136, 203, 228, 266, 282, 302, 363, 462
SfaNI GCATC 1 cut(s) 88
SfcI CTRYAG 1 cut(s) 461
Sfr303I CCGCGG 3 cut(s) 123, 153, 254
SfuI TTCGAA 1 cut(s) 500
SgrBI CCGCGG 3 cut(s) 123, 153, 254
SinI GGWCC 1 cut(s) 453
SmlI CTYRAG 2 cut(s) 443, 524
SmoI CTYRAG 2 cut(s) 443, 524
SspI AATATT 1 cut(s) 439
SspMI CTAG 1 cut(s) 29
StyD4I CCNGG 5 cut(s) 72, 218, 230, 276, 512
StyI CCWWGG 2 cut(s) 105, 507
TaiI ACGT 1 cut(s) 302
TaqI TCGA 4 cut(s) 15, 114, 303, 500
TauI GCSGC 8 cut(s) 48, 83, 86, 104, 122, 125, 253, 573
TfiI GAWTC 2 cut(s) 487, 517
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TseI GCWGC 3 cut(s) 24, 247, 460
VpaK11BI GGWCC 1 cut(s) 453
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.