Rw0G022370

Cgr1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig01034
OG0001180
Physical Location & Seq
Reverse (-)
10572 .. 11021
450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G022370.1

Sequence Viewer

Length: 450 bp
ATGGCTTACACAATTGACTTCCACTGTCTAGACAAAGGCTTCGGCGGCAAAACCTACAAGCGCAAGAGGAACCCCGAAGCCTCCGCCGACGACCAAGATGCCGCCATGGAAATCGACGCCACCTACCTACCTCCGGCAAAGCGATGTATGGTGTCCTTCTCGGACAATCCGGACAAACCAGTCATGATCGCCGAGAAGGTCTCCGAGCTAAAGTGGAAGCAGCCGCGGAAGCAGAGGTCATCGGTGGTTCAGGTGAGTCAGAAGGGAACGACGTTCGAGGAGAGGGAGAAGAACAAGTTGGTGAAGAAGGCGTACAAGGAGAGAATGGTGGAGCTGAAGGGGGAGATTAAGAGGAACAAGGAGGAGAAGAGGAGGAAGAGGGAGGAAATGGAGAAGAAGAAGAAGGAGAATATTCTTCCGGAGGAGATGATCAAGAGGAACAATAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cgr1

Protein Analysis

149

Amino Acids

17.6

Weight (kDa)

9.7

Isoelectric Point (pI)

67.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 179
AccII CGCG 1 cut(s) 226
AccIII TCCGGA 2 cut(s) 169, 418
AciI CCGC 5 cut(s) 45, 84, 102, 224, 226
AcuI CTGAAG 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 117
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 1 cut(s) 133
AjuI GAANNNNNNNTTGG 2 cut(s) 281, 313
AluBI AGCT 2 cut(s) 208, 334
AluI AGCT 2 cut(s) 208, 334
Alw26I GTCTC 1 cut(s) 205
Aor13HI TCCGGA 2 cut(s) 169, 418
ApeKI GCWGC 1 cut(s) 220
ArsI GACNNNNNNTTYG 2 cut(s) 23, 55
AspLEI GCGC 1 cut(s) 63
AsuHPI GGTGA 2 cut(s) 265, 313
BarI GAAGNNNNNNTAC 2 cut(s) 296, 328
BbvI GCAGC 1 cut(s) 232
BcgI CGANNNNNNTGC 2 cut(s) 80, 114
BclI TGATCA 1 cut(s) 429
BcoDI GTCTC 1 cut(s) 205
BfaI CTAG 1 cut(s) 29
BisI GCNGC 4 cut(s) 46, 102, 221, 224
BlsI GCNGC 4 cut(s) 47, 103, 222, 225
BmiI GGNNCC 1 cut(s) 71
BmsI GCATC 1 cut(s) 88
BplI GAGNNNNNCTC 2 cut(s) 185, 217
BsaHI GRCGYC 1 cut(s) 117
BsaI GGTCTC 1 cut(s) 205
BsaJI CCNNGG 2 cut(s) 105, 224
BsaWI WCCGGW 2 cut(s) 169, 418
Bsc4I CCNNNNNNNGG 1 cut(s) 133
Bse1I ACTGG 1 cut(s) 179
BseAI TCCGGA 2 cut(s) 169, 418
BseDI CCNNGG 2 cut(s) 105, 224
BseLI CCNNNNNNNGG 1 cut(s) 133
BseNI ACTGG 1 cut(s) 179
BseRI GAGGAG 4 cut(s) 293, 377, 385, 437
BseXI GCAGC 1 cut(s) 232
Bsh1236I CGCG 1 cut(s) 226
BsiSI CCGG 3 cut(s) 134, 170, 419
BslI CCNNNNNNNGG 1 cut(s) 133
BsmAI GTCTC 1 cut(s) 205
Bso31I GGTCTC 1 cut(s) 205
Bsp13I TCCGGA 2 cut(s) 169, 418
Bsp143I GATC 2 cut(s) 186, 429
Bsp19I CCATGG 1 cut(s) 105
BspACI CCGC 5 cut(s) 45, 84, 102, 224, 226
BspEI TCCGGA 2 cut(s) 169, 418
BspFNI CGCG 1 cut(s) 226
BspHI TCATGA 1 cut(s) 183
BspLI GGNNCC 1 cut(s) 71
BspTNI GGTCTC 1 cut(s) 205
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 2 cut(s) 105, 224
BssMI GATC 2 cut(s) 186, 429
BssNI GRCGYC 1 cut(s) 117
BssT1I CCWWGG 1 cut(s) 105
Bst4CI ACNGT 1 cut(s) 26
Bst6I CTCTTC 2 cut(s) 362, 371
BstACI GRCGYC 1 cut(s) 117
BstDSI CCRYGG 2 cut(s) 105, 224
BstFNI CGCG 1 cut(s) 226
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 2 cut(s) 189, 432
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 2 cut(s) 186, 429
BstMWI GCNNNNNNNGC 2 cut(s) 45, 229
BstUI CGCG 1 cut(s) 226
BstV1I GCAGC 1 cut(s) 232
BtgI CCRYGG 2 cut(s) 105, 224
BtgZI GCGATG 1 cut(s) 157
BtsIMutI CAGTG 1 cut(s) 22
CciI TCATGA 1 cut(s) 183
CfoI GCGC 1 cut(s) 63
Cfr42I CCGCGG 1 cut(s) 227
CseI GACGC 1 cut(s) 125
Csp6I GTAC 1 cut(s) 313
CviAII CATG 2 cut(s) 106, 184
CviJI RGCY 6 cut(s) 5, 39, 80, 208, 223, 334
CviKI_1 RGCY 6 cut(s) 5, 39, 80, 208, 223, 334
CviQI GTAC 1 cut(s) 313
DpnI GATC 2 cut(s) 188, 431
DpnII GATC 2 cut(s) 186, 429
DrdI GACNNNNNNGTC 1 cut(s) 179
DseDI GACNNNNNNGTC 1 cut(s) 179
Eam1104I CTCTTC 2 cut(s) 362, 371
EarI CTCTTC 2 cut(s) 362, 371
EciI GGCGGA 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 105
Eco31I GGTCTC 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 356
EcoT14I CCWWGG 1 cut(s) 105
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 2 cut(s) 109, 187
FaiI YATR 3 cut(s) 107, 149, 185
FatI CATG 2 cut(s) 105, 183
FbaI TGATCA 1 cut(s) 429
Fnu4HI GCNGC 4 cut(s) 46, 102, 221, 224
Fsp4HI GCNGC 4 cut(s) 46, 102, 221, 224
FspBI CTAG 1 cut(s) 29
GlaI GCGC 1 cut(s) 62
GluI GCNGC 4 cut(s) 46, 102, 221, 224
HapII CCGG 3 cut(s) 134, 170, 419
HgaI GACGC 1 cut(s) 125
HhaI GCGC 1 cut(s) 63
Hin1I GRCGYC 1 cut(s) 117
Hin1II CATG 2 cut(s) 109, 187
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HinfI GANTC 1 cut(s) 256
HpaII CCGG 3 cut(s) 134, 170, 419
HphI GGTGA 2 cut(s) 265, 313
Hpy188I TCNGA 3 cut(s) 163, 205, 261
Hpy188III TCNNGA 5 cut(s) 29, 170, 184, 419, 433
Hpy99I CGWCG 3 cut(s) 92, 119, 274
HpyAV CCTTC 6 cut(s) 166, 190, 256, 301, 331, 397
HpyCH4III ACNGT 1 cut(s) 26
HpyCH4IV ACGT 1 cut(s) 272
HpyF10VI GCNNNNNNNGC 2 cut(s) 45, 229
HpySE526I ACGT 1 cut(s) 272
Hsp92I GRCGYC 1 cut(s) 117
Hsp92II CATG 2 cut(s) 109, 187
HspAI GCGC 1 cut(s) 61
Kpn2I TCCGGA 2 cut(s) 169, 418
Ksp22I TGATCA 1 cut(s) 429
KspI CCGCGG 1 cut(s) 227
Kzo9I GATC 2 cut(s) 186, 429
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 5 cut(s) 147, 183, 192, 236, 432
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 1 cut(s) 88
MaeI CTAG 1 cut(s) 29
MaeII ACGT 1 cut(s) 272
MalI GATC 2 cut(s) 188, 431
MboI GATC 2 cut(s) 186, 429
MboII GAAGA 8 cut(s) 301, 316, 379, 388, 406, 407, 409, 412
MfeI CAATTG 1 cut(s) 12
MluCI AATT 1 cut(s) 12
MlyI GAGTC 1 cut(s) 265
MroI TCCGGA 2 cut(s) 169, 418
MseI TTAA 1 cut(s) 348
MspA1I CMGCKG 1 cut(s) 226
MspI CCGG 3 cut(s) 134, 170, 419
MunI CAATTG 1 cut(s) 12
MvnI CGCG 1 cut(s) 226
MwoI GCNNNNNNNGC 2 cut(s) 45, 229
NcoI CCATGG 1 cut(s) 105
NdeII GATC 2 cut(s) 186, 429
NlaIII CATG 2 cut(s) 109, 187
NlaIV GGNNCC 1 cut(s) 71
NmeAIII GCCGAG 1 cut(s) 217
PagI TCATGA 1 cut(s) 183
PkrI GCNGC 4 cut(s) 47, 103, 222, 225
PleI GAGTC 1 cut(s) 264
PpsI GAGTC 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 71
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SacII CCGCGG 1 cut(s) 227
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 4 cut(s) 46, 102, 221, 224
Sau3AI GATC 2 cut(s) 186, 429
SchI GAGTC 1 cut(s) 265
SfaNI GCATC 1 cut(s) 88
Sfr303I CCGCGG 1 cut(s) 227
SgrBI CCGCGG 1 cut(s) 227
Sse9I AATT 1 cut(s) 12
SsiI CCGC 5 cut(s) 45, 84, 102, 224, 226
SspI AATATT 1 cut(s) 412
SspMI CTAG 1 cut(s) 29
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 1 cut(s) 26
TaiI ACGT 1 cut(s) 275
TaqI TCGA 2 cut(s) 114, 276
TasI AATT 1 cut(s) 12
TauI GCSGC 3 cut(s) 48, 104, 226
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 1 cut(s) 29
TseI GCWGC 1 cut(s) 220
TspRI CASTG 1 cut(s) 29
XbaI TCTAGA 1 cut(s) 28
XspI CTAG 1 cut(s) 29
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.