Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6D

Orthogroup ID

OG0001641

Physical Location & Seq

Genomic Coordinates Forward (+)

40303303 .. 40322447

Gene Structure 19145 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6DG224900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2691 bp

ATGTCATTAACCAAAGTTTCTCGTGATTACAAAAACAGGATTTATGCAAAAACCATGATGTCAAACCAGCCCTGTTGGTTGAACTGCTCCTCTGGTAACAGAGATTCAAGTGCTATGCTAGAAAGAGATGGGACCCTACTTTTGGTTCCAACTGCAGGAGGATTAATGATTGAGCTGTTCGTCTCCAAAGATGTCTCTGAGAACCAGCAAGTCATTGATTTCATCACAGCACAATGCAACATTTGGCGCGAGCAAGACACCCTGATCAGTGCAAGCAACACATGTAGTACAAACACCCCTGTACTTTCCCCATCAATGGCATCATTGGATCCTCATGATGCGAATTTGCCATACGATATCACAGTTGATCGAATCCGCCTATGCTCTTCTGAGTCCCCTTCTCCATTGAACTTCCTGCAACATGTCACTAGTTACAACTCTGACAACAGAATGAAGAACAGTACTAGTGAATGTAATTATGACCAGGGTAATTCACATGAGCACTCTTTGGATCAGCCATTAGAAAACATGGAGCAACAAGGTACTCTGGATTATCAGGATTCGATAAAACCAGCAGACCATTCAGTTTCAGATTGCAGTGACCAAACTGATGACACAAATTATAAAACAAGAAATGGAAAAGAGCCTCAATCCAAAAACCTTGTTGCTGAGAGGAAGAGAAGAAAGAAGCTTAACGAAAAACTCTATAAACTTCGCTCTTTGGTTCCCAACATTTCTAAGATGGACAAGGCTTCGATTCTCAAGGATGCGATTGATTTTGTCAAGGACTTGCAGAGACAAGTGAAAGAACTCCAAGATGTGCTTGAACATAATTCAAATGAAGATGGAGGAGCTAAGAGAACTTTTAATAGTATCTCTGGAAATCACAACAATTACCAGTCAGAGGGTCTGAATCAACATGGAATTTCCATGGCAAATAAACCAAAAAGTGACAACCCTCAAAACGGCTTTCGTGACTCGGATCATAATACTAATGAGAAGGGACAACAGCAGATGGAGCCACAAGTGGGAGTAACTCAGTTAGACGGCAACGAGTTCTTTGTGACAGTGTTCTGTGAGCAGAAGCCAGAAGGATTTGTGAGACTGATGGAGGCATTGGATAGTTTAGGCTTGGAAGTGACTAATGCAAACGTTACTAGCTTCAGAAGCCTTGTCTCTAATGTGTTTAAAGTAGAGCGTGTGACTCCTAGCCACTTTTCTTGTAGTGAATCACATAAGATTCAGGACGTACTTGAAGACCAGAATGTTATCGATTTCATCACAGAGCAATACAACATCTCAATAGAACAGGATCATATTGGTACAAGCTTTGGGGTAAACTTGGACACAATGATTCATGATGAAATCCAATCAAAGCCAGTACTTTTAGACCACGAACAAATGGATCCCAGCAATCATGTTTTCTGCTCTGGCGATAATTCTGCCATGAACTTCTTGCAGCAGTTTAGTCACAACTTGGGAAGCAGGATGAAGAACATTAGTTATAATGTCAATTCTTTTGAAGATTCATCACATGCACCATTTCTTTCTGATAAGCAAATGGAGCACCCATTTACTAATTGTTCGAAAAAAACTGGGTTTGATCAAGCAACGGAGGCAATGCAAAGGTCCATGATGATGAGTGAGACACAAGCCACACACCATATGCATATGCAATATATGGATCAGTCATTAGAACCAGGCATGGATCATGAGCTGGAGCAGGATGCCGGCAATAATGATAAGGACTCAAATATTATAAATGATGATGGGGGACATGCTACAGATTTGAACTCAGATTGCAGTGATCATGATCAGATTGATGCTGAGGATGATGCAAAATATCGGAGGAGGAACGGAAAAGGGCAGGCCAAAAACCTTGTTGCAGAGAGAAAGAGAAGGAAGAAGCTTAATGACAGGCTCTATGAACTTAGGTCTTTAGTTCCCAATATTTCCAAGTTGGATAGATCTTCTATTCTTGGGGATGCAATAGAGTATGTGATGGAGTTGCAGAACCAAGCTAAAAAACTTCAAGATGAGCTTGATGATGATCATGATCACGAAGAAGTTCGCCATGTTCATGAAGGTGCTGCTAATAAGAACTGTGGCGTCACTTTCCAACATAACAGTTTACAATCAGAGATTGATCAGATTCAAAGTGGGGTTGACCTTAAAACTGAGCAAAATGATCATAAAGCTCCAAATGGGTTTCATTTGGGAACATCAGGAGACGGTAGTGTCTCAAAACAAAGCCCCGATTCAGAAGTTATCCACGATCACAAGACACAGCAGATGGAGCCCCAAGTGGAAGTAGCTCAAATAGATGGGAATCAGTTCTTTGTGAAAGTATTCTGTGAGCACAAGCCTGGTGGATTTGTGAGATTGATGGAGGCATTGAGCTCTCTGAGCCTGGAAGTTACTAATGCCAATGTAACTAGCTTCAGATGCCTCGTCTCTAATGTCTTCATCGTAGAGAAGAAGGATAGTGAACTAGTTGAAGCTGATGAAGTGAGAGACTCGTTGCTAGAGCTAACAAGGAACCCCTTAAAATGGCGTAGTGAGGAAATGGCGAAAGCATCAGAGATTGGGATTGGCTTGGATAACAACCACCACCATCATCCCCATCACCAAAACCTCCACAACCACCATTTCAGTCCCTTCCACTTGCACCATCTTCCTACTGAAGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000987 GO:0001012 GO:0001067 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003682 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0007275 GO:0008150 GO:0009555 GO:0009653 GO:0009791 GO:0009889 GO:0009908 GO:0009987 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0022414 GO:0030154 GO:0031323 GO:0031326 GO:0032501 GO:0032502 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044424 GO:0044464 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048444 GO:0048448 GO:0048449 GO:0048455 GO:0048466 GO:0048608 GO:0048646 GO:0048653 GO:0048654 GO:0048655 GO:0048656 GO:0048657 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0061458 GO:0065007 GO:0080090 GO:0090567 GO:0090696 GO:0090697 GO:0090698 GO:0097159 GO:0099402 GO:0140110 GO:1901363 GO:1903506 GO:1905392 GO:1905393 GO:1990837 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

ko00040 ko00053 ko00520 ko01100 map00040 map00053 map00520 map01100

Pfam Domains

Protein Families

HLH bHLH-MYC_N

Protein Analysis

896

Amino Acids

101.33

Weight (kDa)

5.27

Isoelectric Point (pI)

50.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HLH	PF00010	222 - 265	8.2e-11	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	342 - 399	1.4e-09	Plant bHLH transcription factor, ACT-like domain
HLH	PF00010	628 - 671	1e-10	Helix-loop-helix DNA-binding domain
bHLH-TF_ACT-like_plant	PF22754	768 - 842	8.9e-12	Plant bHLH transcription factor, ACT-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0001641)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G16910
fragaria_vesca	FvH4_2g15460 FvH4_2g15460 FvH4_2g15470
malus_domestica	MD05G1122900.v1.1 MD05G1123000.v1.1 MD10G1125700.v1.1
prunus_persica	Prupe.8G166800_v2.0.a1 Prupe.8G166800_v2.0.a1 Prupe.8G166800_v2.0.a1 Prupe.8G166800_v2.0.a1 Prupe.8G166800_v2.0.a1 Prupe.8G166900_v2.0.a1
pyrus_communis	pycom05g11720 pycom05g11750 pycom10g10820
rosa_chinensis	RchiOBHm_Chr6g0278441 RchiOBHm_Chr6g0278471
rosa_laevigata	RLG00000013226 RLG00000013228 RLG00000013381
rosa_multiflora	Rmu_sc0000258.1_g000003 Rmu_sc0000258.1_g000004
rosa_roxburghii	Rroxscaffold_3G00229220 Rroxscaffold_7G00189870 Rroxscaffold_7G00189880 Rroxscaffold_7G00190350
rosa_rugosa	Rorug06G0006400 Rorug06G0117000 Rorug06G0117100.1
rosa_samantha	Rh1DG021000 Rh2DG345500 Rh2DG346800 Rh3AG263900 Rh3CG365900 Rh3DG372000 Rh5CG269300 Rh6AG226300 Rh6BG230500 Rh6CG233600 Rh6DG224900 Rh7AG412300 Rh7CG321300 Rh7DG336100
rosa_wichuraiana	Rw0G018390 Rw3G029150 Rw5G034840 Rw6G019740 Rw6G019750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	624, 1506, 1760
AasI	GACNNNNNNGTC	1 cut(s)	2237
AccII	CGCG	1 cut(s)	249
AciI	CCGC	1 cut(s)	376
AclI	AACGTT	1 cut(s)	1152
AclWI	GGATC	9 cut(s)	323, 336, 519, 990, 1320, 1400, 1413, 1692, 1716
AcsI	RAATTY	2 cut(s)	343, 924
AcuI	CTGAAG	2 cut(s)	1147, 2425
AcyI	GRCGYC	1 cut(s)	2109
AfaI	GTAC	7 cut(s)	289, 303, 463, 544, 1251, 1324, 1383
AfiI	CCNNNNNNNGG	7 cut(s)	142, 155, 316, 904, 965, 1028, 2550
AflIII	ACRYGT	2 cut(s)	281, 421
AhlI	ACTAGT	3 cut(s)	428, 464, 2491
AjnI	CCWGG	4 cut(s)	483, 1699, 2365, 2409
Alw21I	GWGCWC	4 cut(s)	504, 1569, 2361, 2402
AlwI	GGATC	9 cut(s)	323, 336, 519, 990, 1320, 1400, 1413, 1692, 1716
AoxI	GGCC	1 cut(s)	1869
ApeKI	GCWGC	2 cut(s)	1459, 2090
ApoI	RAATTY	2 cut(s)	343, 924
ArsI	GACNNNNNNTTYG	2 cut(s)	124, 156
AseI	ATTAAT	1 cut(s)	164
Asp700I	GAANNNNTTC	3 cut(s)	2067, 2333, 2348
AspLEI	GCGC	1 cut(s)	249
AspS9I	GGNCC	2 cut(s)	132, 1629
AsuHPI	GGTGA	1 cut(s)	2618
AsuII	TTCGAA	1 cut(s)	1586
AvaII	GGWCC	2 cut(s)	132, 1629
BamHI	GGATCC	2 cut(s)	328, 1405
BanII	GRGCYC	2 cut(s)	2301, 2402
BauI	CACGAG	1 cut(s)	21
BbsI	GAAGAC	2 cut(s)	1263, 2455
Bbv12I	GWGCWC	4 cut(s)	504, 1569, 2361, 2402
BbvCI	CCTCAGC	1 cut(s)	1827
BbvI	GCAGC	2 cut(s)	1471, 2077
BceAI	ACGGC	2 cut(s)	982, 1063
BcgI	CGANNNNNNTGC	2 cut(s)	1424, 1458
BciT130I	CCWGG	4 cut(s)	485, 1701, 2367, 2411
BclI	TGATCA	8 cut(s)	264, 1603, 1807, 1813, 2050, 2056, 2146, 2188
BcuI	ACTAGT	3 cut(s)	428, 464, 2491
BfaI	CTAG	8 cut(s)	119, 429, 465, 1158, 1209, 2436, 2492, 2525
BfmI	CTRYAG	2 cut(s)	153, 1782
BglII	AGATCT	1 cut(s)	1967
BisI	GCNGC	2 cut(s)	1460, 2091
BlsI	GCNGC	2 cut(s)	1461, 2092
BmcAI	AGTACT	2 cut(s)	463, 1383
Bme1390I	CCNGG	4 cut(s)	485, 1701, 2367, 2411
Bme18I	GGWCC	2 cut(s)	132, 1629
BmgT120I	GGNCC	2 cut(s)	132, 1629
BmiI	GGNNCC	9 cut(s)	133, 134, 147, 330, 726, 1020, 1407, 2298, 2540
BmrFI	CCNGG	4 cut(s)	485, 1701, 2367, 2411
BmrI	ACTGGG	1 cut(s)	1605
BmsI	GCATC	9 cut(s)	328, 329, 757, 1717, 1813, 1825, 1975, 2435, 2585
BmuI	ACTGGG	1 cut(s)	1605
BpiI	GAAGAC	2 cut(s)	1263, 2455
BpmI	CTGGAG	1 cut(s)	1739
Bpu10I	CCTNAGC	1 cut(s)	1827
Bpu14I	TTCGAA	1 cut(s)	1586
BpuEI	CTTGAG	1 cut(s)	746
Bsa29I	ATCGAT	1 cut(s)	1272
BsaBI	GATNNNNATC	4 cut(s)	1812, 2049, 2055, 2328
BsaHI	GRCGYC	1 cut(s)	2109
BsaJI	CCNNGG	2 cut(s)	484, 930
Bsc4I	CCNNNNNNNGG	7 cut(s)	142, 155, 316, 904, 965, 1028, 2550
Bse118I	RCCGGY	1 cut(s)	1730
Bse1I	ACTGG	3 cut(s)	898, 1379, 1600
Bse3DI	GCAATG	1 cut(s)	1626
Bse8I	GATNNNNATC	4 cut(s)	1812, 2049, 2055, 2328
BseBI	CCWGG	4 cut(s)	485, 1701, 2367, 2411
BseCI	ATCGAT	1 cut(s)	1272
BseDI	CCNNGG	2 cut(s)	484, 930
BseGI	GGATG	6 cut(s)	772, 1494, 1732, 1837, 1990, 2617
BseJI	GATNNNNATC	4 cut(s)	1812, 2049, 2055, 2328
BseLI	CCNNNNNNNGG	7 cut(s)	142, 155, 316, 904, 965, 1028, 2550
BseMI	GCAATG	1 cut(s)	1626
BseMII	CTCAG	8 cut(s)	189, 381, 660, 1052, 1809, 1818, 2169, 2396
BseNI	ACTGG	3 cut(s)	898, 1379, 1600
BseRI	GAGGAG	3 cut(s)	79, 864, 1864
BseXI	GCAGC	2 cut(s)	1471, 2077
BseYI	CCCAGC	1 cut(s)	1409
Bsh1236I	CGCG	1 cut(s)	249
BshFI	GGCC	1 cut(s)	1871
BshVI	ATCGAT	1 cut(s)	1272
BsiHKAI	GWGCWC	4 cut(s)	504, 1569, 2361, 2402
BsiSI	CCGG	1 cut(s)	1731
BslFI	GGGAC	5 cut(s)	145, 379, 1017, 1788, 2640
BslI	CCNNNNNNNGG	7 cut(s)	142, 155, 316, 904, 965, 1028, 2550
BsmBI	CGTCTC	3 cut(s)	187, 2223, 2458
BsmFI	GGGAC	5 cut(s)	145, 379, 1017, 1788, 2640
BsnI	GGCC	1 cut(s)	1871
Bsp119I	TTCGAA	1 cut(s)	1586
Bsp1286I	GDGCHC	5 cut(s)	504, 1569, 2301, 2361, 2402
Bsp19I	CCATGG	1 cut(s)	930
BspACI	CCGC	1 cut(s)	376
BspANI	GGCC	1 cut(s)	1871
BspCNI	CTCAG	8 cut(s)	190, 382, 661, 1051, 1808, 1819, 2170, 2397
BspDI	ATCGAT	1 cut(s)	1272
BspFNI	CGCG	1 cut(s)	249
BspHI	TCATGA	6 cut(s)	334, 1357, 1711, 1810, 2053, 2080
BspLI	GGNNCC	9 cut(s)	133, 134, 147, 330, 726, 1020, 1407, 2298, 2540
BspMAI	CTGCAG	1 cut(s)	157
BspPI	GGATC	9 cut(s)	323, 336, 519, 990, 1320, 1400, 1413, 1692, 1716
BspQI	GCTCTTC	1 cut(s)	391
BspT104I	TTCGAA	1 cut(s)	1586
BsrDI	GCAATG	1 cut(s)	1626
BsrFI	RCCGGY	1 cut(s)	1730
BsrI	ACTGG	3 cut(s)	898, 1379, 1600
BssAI	RCCGGY	1 cut(s)	1730
BssECI	CCNNGG	2 cut(s)	484, 930
BssNI	GRCGYC	1 cut(s)	2109
BssSI	CACGAG	1 cut(s)	21
BssT1I	CCWWGG	1 cut(s)	930
Bst2BI	CACGAG	1 cut(s)	21
Bst2UI	CCWGG	4 cut(s)	485, 1701, 2367, 2411
Bst4CI	ACNGT	6 cut(s)	364, 461, 1069, 2105, 2129, 2234
Bst6I	CTCTTC	2 cut(s)	391, 671
BstACI	GRCGYC	1 cut(s)	2109
BstBI	TTCGAA	1 cut(s)	1586
BstC8I	GCNNGC	4 cut(s)	251, 274, 1732, 1869
BstDSI	CCRYGG	1 cut(s)	930
BstF5I	GGATG	6 cut(s)	772, 1494, 1732, 1837, 1990, 2617
BstFNI	CGCG	1 cut(s)	249
BstHHI	GCGC	1 cut(s)	249
BstMWI	GCNNNNNNNGC	7 cut(s)	1018, 1167, 1564, 1616, 2296, 2406, 2445
BstNI	CCWGG	4 cut(s)	485, 1701, 2367, 2411
BstNSI	RCATGY	4 cut(s)	285, 425, 1538, 1781
BstSCI	CCNGG	4 cut(s)	483, 1699, 2365, 2409
BstSFI	CTRYAG	2 cut(s)	153, 1782
BstUI	CGCG	1 cut(s)	249
BstV1I	GCAGC	2 cut(s)	1471, 2077
BstV2I	GAAGAC	2 cut(s)	1263, 2455
BstX2I	RGATCY	3 cut(s)	328, 1405, 1967
BstYI	RGATCY	3 cut(s)	328, 1405, 1967
Bsu15I	ATCGAT	1 cut(s)	1272
BsuRI	GGCC	1 cut(s)	1871
BsuTUI	ATCGAT	1 cut(s)	1272
BtgI	CCRYGG	1 cut(s)	930
BtsCI	GGATG	6 cut(s)	772, 1494, 1732, 1837, 1990, 2617
BtsI	GCAGTG	2 cut(s)	604, 1810
BtsIMutI	CAGTG	4 cut(s)	274, 604, 1074, 1810
Cac8I	GCNNGC	4 cut(s)	251, 274, 1732, 1869
CciI	TCATGA	6 cut(s)	334, 1357, 1711, 1810, 2053, 2080
CfoI	GCGC	1 cut(s)	249
Cfr10I	RCCGGY	1 cut(s)	1730
Cfr13I	GGNCC	2 cut(s)	132, 1629
ClaI	ATCGAT	1 cut(s)	1272
CseI	GACGC	1 cut(s)	2098
Csp6I	GTAC	7 cut(s)	288, 302, 462, 543, 1250, 1323, 1382
CspCI	CAANNNNNGTGG	4 cut(s)	1202, 1237, 2350, 2385
CviQI	GTAC	7 cut(s)	288, 302, 462, 543, 1250, 1323, 1382
DraI	TTTAAA	1 cut(s)	1189
DrdI	GACNNNNNNGTC	1 cut(s)	2237
DseDI	GACNNNNNNGTC	1 cut(s)	2237
Eam1104I	CTCTTC	2 cut(s)	391, 671
EarI	CTCTTC	2 cut(s)	391, 671
EciI	GGCGGA	1 cut(s)	365
Ecl136II	GAGCTC	1 cut(s)	2400
Eco130I	CCWWGG	1 cut(s)	930
Eco24I	GRGCYC	2 cut(s)	2301, 2402
Eco32I	GATATC	1 cut(s)	358
Eco47I	GGWCC	2 cut(s)	132, 1629
Eco53kI	GAGCTC	1 cut(s)	2400
Eco57I	CTGAAG	2 cut(s)	1147, 2425
EcoICRI	GAGCTC	1 cut(s)	2400
EcoO109I	RGGNCCY	1 cut(s)	132
EcoRII	CCWGG	4 cut(s)	483, 1699, 2365, 2409
EcoRV	GATATC	1 cut(s)	358
EcoT14I	CCWWGG	1 cut(s)	930
EcoT22I	ATGCAT	1 cut(s)	1671
EcoT38I	GRGCYC	2 cut(s)	2301, 2402
ErhI	CCWWGG	1 cut(s)	930
Esp3I	CGTCTC	3 cut(s)	187, 2223, 2458
FalI	AAGNNNNNCTT	4 cut(s)	807, 839, 2025, 2057
FaqI	GGGAC	5 cut(s)	145, 379, 1017, 1788, 2640
FauNDI	CATATG	2 cut(s)	1665, 1671
FbaI	TGATCA	8 cut(s)	264, 1603, 1807, 1813, 2050, 2056, 2146, 2188
Fnu4HI	GCNGC	2 cut(s)	1460, 2091
FokI	GGATG	6 cut(s)	779, 1501, 1739, 1844, 1997, 2604
FriOI	GRGCYC	2 cut(s)	2301, 2402
Fsp4HI	GCNGC	2 cut(s)	1460, 2091
FspBI	CTAG	8 cut(s)	119, 429, 465, 1158, 1209, 2436, 2492, 2525
GlaI	GCGC	1 cut(s)	248
GluI	GCNGC	2 cut(s)	1460, 2091
GsaI	CCCAGC	1 cut(s)	1413
GsuI	CTGGAG	1 cut(s)	1739
HaeIII	GGCC	1 cut(s)	1871
HapII	CCGG	1 cut(s)	1731
HgaI	GACGC	1 cut(s)	2098
HhaI	GCGC	1 cut(s)	249
Hin1I	GRCGYC	1 cut(s)	2109
Hin6I	GCGC	1 cut(s)	247
HinP1I	GCGC	1 cut(s)	247
HincII	GTYRAC	1 cut(s)	2167
HindII	GTYRAC	1 cut(s)	2167
HindIII	AAGCTT	3 cut(s)	689, 1327, 1907
HpaII	CCGG	1 cut(s)	1731
HphI	GGTGA	1 cut(s)	2618
Hpy166II	GTNNAC	4 cut(s)	1339, 2132, 2167, 2489
Hpy8I	GTNNAC	4 cut(s)	1339, 2132, 2167, 2489
HpyAV	CCTTC	7 cut(s)	408, 994, 1085, 1893, 2078, 2473, 2668
HpyCH4III	ACNGT	6 cut(s)	364, 461, 1069, 2105, 2129, 2234
HpyCH4IV	ACGT	2 cut(s)	1152, 1248
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1018, 1167, 1564, 1616, 2296, 2406, 2445
HpySE526I	ACGT	2 cut(s)	1152, 1248
Hsp92I	GRCGYC	1 cut(s)	2109
HspAI	GCGC	1 cut(s)	247
KflI	GGGWCCC	1 cut(s)	132
KroI	GCCGGC	1 cut(s)	1730
KroNI	GCCGGC	1 cut(s)	1732
Ksp22I	TGATCA	8 cut(s)	264, 1603, 1807, 1813, 2050, 2056, 2146, 2188
LguI	GCTCTTC	1 cut(s)	391
LmnI	GCTCC	8 cut(s)	92, 532, 851, 1018, 1564, 1720, 2203, 2296
Lsp1109I	GCAGC	2 cut(s)	1471, 2077
LweI	GCATC	9 cut(s)	328, 329, 757, 1717, 1813, 1825, 1975, 2435, 2585
MaeI	CTAG	8 cut(s)	119, 429, 465, 1158, 1209, 2436, 2492, 2525
MaeII	ACGT	2 cut(s)	1152, 1248
MflI	RGATCY	3 cut(s)	328, 1405, 1967
MhlI	GDGCHC	5 cut(s)	504, 1569, 2301, 2361, 2402
MlyI	GAGTC	5 cut(s)	401, 971, 1198, 1742, 2510
MmeI	TCCRAC	3 cut(s)	173, 1941, 2143
Mph1103I	ATGCAT	1 cut(s)	1671
MroNI	GCCGGC	1 cut(s)	1730
MroXI	GAANNNNTTC	3 cut(s)	2067, 2333, 2348
MseI	TTAA	8 cut(s)	8, 164, 693, 867, 1188, 1911, 2172, 2546
MslI	CAYNNNNRTG	3 cut(s)	1637, 2079, 2085
MspI	CCGG	1 cut(s)	1731
MspR9I	CCNGG	4 cut(s)	485, 1701, 2367, 2411
MvaI	CCWGG	4 cut(s)	485, 1701, 2367, 2411
MvnI	CGCG	1 cut(s)	249
MwoI	GCNNNNNNNGC	7 cut(s)	1018, 1167, 1564, 1616, 2296, 2406, 2445
NaeI	GCCGGC	1 cut(s)	1732
NcoI	CCATGG	1 cut(s)	930
NdeI	CATATG	2 cut(s)	1665, 1671
NgoMIV	GCCGGC	1 cut(s)	1730
NlaIV	GGNNCC	9 cut(s)	133, 134, 147, 330, 726, 1020, 1407, 2298, 2540
NmuCI	GTSAC	9 cut(s)	424, 599, 950, 974, 1063, 1138, 1201, 1469, 2110
NsiI	ATGCAT	1 cut(s)	1671
NspI	RCATGY	4 cut(s)	285, 425, 1538, 1781
NspV	TTCGAA	1 cut(s)	1586
PagI	TCATGA	6 cut(s)	334, 1357, 1711, 1810, 2053, 2080
PciI	ACATGT	2 cut(s)	281, 421
PciSI	GCTCTTC	1 cut(s)	391
PdiI	GCCGGC	1 cut(s)	1732
PdmI	GAANNNNTTC	3 cut(s)	2067, 2333, 2348
PkrI	GCNGC	2 cut(s)	1461, 2092
PleI	GAGTC	5 cut(s)	400, 971, 1198, 1742, 2510
PpsI	GAGTC	5 cut(s)	400, 971, 1198, 1742, 2510
PpuMI	RGGWCCY	1 cut(s)	132
PscI	ACATGT	2 cut(s)	281, 421
PshBI	ATTAAT	1 cut(s)	164
PsiI	TTATAA	3 cut(s)	624, 1506, 1760
Psp124BI	GAGCTC	1 cut(s)	2402
Psp1406I	AACGTT	1 cut(s)	1152
Psp5II	RGGWCCY	1 cut(s)	132
Psp6I	CCWGG	4 cut(s)	483, 1699, 2365, 2409
PspFI	CCCAGC	1 cut(s)	1409
PspGI	CCWGG	4 cut(s)	483, 1699, 2365, 2409
PspN4I	GGNNCC	9 cut(s)	133, 134, 147, 330, 726, 1020, 1407, 2298, 2540
PspPI	GGNCC	2 cut(s)	132, 1629
PspPPI	RGGWCCY	1 cut(s)	132
PstI	CTGCAG	1 cut(s)	157
PsuI	RGATCY	3 cut(s)	328, 1405, 1967
RsaI	GTAC	7 cut(s)	289, 303, 463, 544, 1251, 1324, 1383
RsaNI	GTAC	7 cut(s)	288, 302, 462, 543, 1250, 1323, 1382
RseI	CAYNNNNRTG	3 cut(s)	1637, 2079, 2085
SacI	GAGCTC	1 cut(s)	2402
SapI	GCTCTTC	1 cut(s)	391
SaqAI	TTAA	8 cut(s)	8, 164, 693, 867, 1188, 1911, 2172, 2546
SatI	GCNGC	2 cut(s)	1460, 2091
Sau96I	GGNCC	2 cut(s)	132, 1629
ScaI	AGTACT	2 cut(s)	463, 1383
SchI	GAGTC	5 cut(s)	401, 971, 1198, 1742, 2510
ScrFI	CCNGG	4 cut(s)	485, 1701, 2367, 2411
SduI	GDGCHC	5 cut(s)	504, 1569, 2301, 2361, 2402
SfaNI	GCATC	9 cut(s)	328, 329, 757, 1717, 1813, 1825, 1975, 2435, 2585
SfcI	CTRYAG	2 cut(s)	153, 1782
SfuI	TTCGAA	1 cut(s)	1586
SinI	GGWCC	2 cut(s)	132, 1629
SmiMI	CAYNNNNRTG	3 cut(s)	1637, 2079, 2085
SmlI	CTYRAG	1 cut(s)	761
SmoI	CTYRAG	1 cut(s)	761
SpeI	ACTAGT	3 cut(s)	428, 464, 2491
SsiI	CCGC	1 cut(s)	376
SspI	AATATT	2 cut(s)	1756, 1951
SspMI	CTAG	8 cut(s)	119, 429, 465, 1158, 1209, 2436, 2492, 2525
SstI	GAGCTC	1 cut(s)	2402
StyD4I	CCNGG	4 cut(s)	483, 1699, 2365, 2409
StyI	CCWWGG	1 cut(s)	930
TaaI	ACNGT	6 cut(s)	364, 461, 1069, 2105, 2129, 2234
TaiI	ACGT	2 cut(s)	1155, 1251
TaqI	TCGA	5 cut(s)	370, 563, 755, 1272, 1586
TatI	WGTACW	4 cut(s)	287, 301, 461, 1381
Tru1I	TTAA	8 cut(s)	8, 164, 693, 867, 1188, 1911, 2172, 2546
Tru9I	TTAA	8 cut(s)	8, 164, 693, 867, 1188, 1911, 2172, 2546
TscAI	CASTG	4 cut(s)	274, 604, 1074, 1810
TseFI	GTSAC	9 cut(s)	424, 599, 950, 974, 1063, 1138, 1201, 1469, 2110
TseI	GCWGC	2 cut(s)	1459, 2090
Tsp45I	GTSAC	9 cut(s)	424, 599, 950, 974, 1063, 1138, 1201, 1469, 2110
TspGWI	ACGGA	2 cut(s)	1628, 1872
TspRI	CASTG	4 cut(s)	274, 604, 1074, 1810
VpaK11BI	GGWCC	2 cut(s)	132, 1629
VspI	ATTAAT	1 cut(s)	164
XapI	RAATTY	2 cut(s)	343, 924
XceI	RCATGY	4 cut(s)	285, 425, 1538, 1781
XmnI	GAANNNNTTC	3 cut(s)	2067, 2333, 2348
XspI	CTAG	8 cut(s)	119, 429, 465, 1158, 1209, 2436, 2492, 2525
ZrmI	AGTACT	2 cut(s)	463, 1383
Zsp2I	ATGCAT	1 cut(s)	1671

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.