Rw1G008650

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001802
Physical Location & Seq
Forward (+)
18894395 .. 18895612
1218 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G008650.1

Sequence Viewer

Length: 339 bp
ATGGAGATAAAGAAAGCAGAGCAGCTTGAAACCGTGGCCTCACTTATAATCCCTGGAGGAGAGAGCACTACCATGGCTAAGCTCGCCAAGTACCACAACCTGGTTGCATTTGAGCTCGCCTTCTTCTTTTGGGTTCCAGCCCAATTCGAAGTAAAATTGTGCTACCGCGAGCACACCGTGATCATTGTTACAAGAGTTTTCTTAGCGATGACCACCCAAGTCCTCTGCAGCTACATTACTCTTCCTTGTAACACAGAGTATGATGCTGATGGTGGGACTGAAGGTTTTCAAGGACATGCCAGGGTCATTATGCCAGGGATCAGTACATGCATGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

112

Amino Acids

12.5

Weight (kDa)

5.51

Isoelectric Point (pI)

24.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SNO PF01174 2 - 35 9.8e-08 SNO glutamine amidotransferase family
Mlo PF03094 36 - 83 9.1e-14 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 47
AccB7I CCANNNNNTGG 1 cut(s) 100
AccII CGCG 1 cut(s) 168
AciI CCGC 1 cut(s) 166
AclWI GGATC 1 cut(s) 326
AcuI CTGAAG 1 cut(s) 300
AdeI CACNNNGTG 1 cut(s) 178
AfaI GTAC 2 cut(s) 92, 325
AfiI CCNNNNNNNGG 1 cut(s) 100
AgsI TTSAA 2 cut(s) 29, 290
AjnI CCWGG 4 cut(s) 52, 99, 299, 313
AluBI AGCT 4 cut(s) 25, 82, 115, 231
AluI AGCT 4 cut(s) 25, 82, 115, 231
Alw21I GWGCWC 3 cut(s) 68, 117, 174
AlwI GGATC 1 cut(s) 326
AoxI GGCC 1 cut(s) 36
ApeKI GCWGC 2 cut(s) 22, 228
Asp700I GAANNNNTTC 1 cut(s) 285
AsuII TTCGAA 1 cut(s) 147
BanII GRGCYC 1 cut(s) 117
Bbv12I GWGCWC 3 cut(s) 68, 117, 174
BbvI GCAGC 2 cut(s) 34, 240
BccI CCATC 1 cut(s) 263
BciT130I CCWGG 4 cut(s) 54, 101, 301, 315
BclI TGATCA 1 cut(s) 180
BfmI CTRYAG 1 cut(s) 226
BisI GCNGC 2 cut(s) 23, 229
BlpI GCTNAGC 1 cut(s) 78
BlsI GCNGC 2 cut(s) 24, 230
Bme1390I CCNGG 4 cut(s) 54, 101, 301, 315
BmiI GGNNCC 1 cut(s) 135
BmrFI CCNGG 4 cut(s) 54, 101, 301, 315
BmsI GCATC 1 cut(s) 253
BpmI CTGGAG 1 cut(s) 75
Bpu1102I GCTNAGC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 147
BsaJI CCNNGG 5 cut(s) 33, 52, 72, 300, 314
Bsc4I CCNNNNNNNGG 1 cut(s) 100
BseBI CCWGG 4 cut(s) 54, 101, 301, 315
BseDI CCNNGG 5 cut(s) 33, 52, 72, 300, 314
BseLI CCNNNNNNNGG 1 cut(s) 100
BseRI GAGGAG 1 cut(s) 72
BseXI GCAGC 2 cut(s) 34, 240
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 1 cut(s) 38
BsiHKAI GWGCWC 3 cut(s) 68, 117, 174
BslFI GGGAC 1 cut(s) 289
BslI CCNNNNNNNGG 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 289
BsnI GGCC 1 cut(s) 38
Bsp119I TTCGAA 1 cut(s) 147
Bsp1286I GDGCHC 3 cut(s) 68, 117, 174
Bsp143I GATC 2 cut(s) 180, 318
Bsp1720I GCTNAGC 1 cut(s) 78
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 1 cut(s) 166
BspANI GGCC 1 cut(s) 38
BspFNI CGCG 1 cut(s) 168
BspLI GGNNCC 1 cut(s) 135
BspMAI CTGCAG 1 cut(s) 230
BspPI GGATC 1 cut(s) 326
BspT104I TTCGAA 1 cut(s) 147
BssECI CCNNGG 5 cut(s) 33, 52, 72, 300, 314
BssMI GATC 2 cut(s) 180, 318
BssT1I CCWWGG 1 cut(s) 72
Bst2UI CCWGG 4 cut(s) 54, 101, 301, 315
Bst4CI ACNGT 2 cut(s) 34, 178
Bst6I CTCTTC 1 cut(s) 246
BstBI TTCGAA 1 cut(s) 147
BstC8I GCNNGC 4 cut(s) 84, 117, 170, 332
BstDEI CTNAG 2 cut(s) 78, 202
BstDSI CCRYGG 2 cut(s) 33, 72
BstFNI CGCG 1 cut(s) 168
BstKTI GATC 2 cut(s) 183, 321
BstMBI GATC 2 cut(s) 180, 318
BstMWI GCNNNNNNNGC 1 cut(s) 83
BstNI CCWGG 4 cut(s) 54, 101, 301, 315
BstNSI RCATGY 3 cut(s) 299, 330, 334
BstSCI CCNGG 4 cut(s) 52, 99, 299, 313
BstSFI CTRYAG 1 cut(s) 226
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 34, 240
BsuRI GGCC 1 cut(s) 38
BtgI CCRYGG 2 cut(s) 33, 72
BtgZI GCGATG 1 cut(s) 221
Cac8I GCNNGC 4 cut(s) 84, 117, 170, 332
CsiI ACCWGGT 1 cut(s) 99
Csp6I GTAC 2 cut(s) 91, 324
CviAII CATG 4 cut(s) 73, 296, 327, 331
CviJI RGCY 7 cut(s) 25, 38, 77, 82, 115, 140, 231
CviKI_1 RGCY 7 cut(s) 25, 38, 77, 82, 115, 140, 231
CviQI GTAC 2 cut(s) 91, 324
DdeI CTNAG 2 cut(s) 78, 202
DpnI GATC 2 cut(s) 182, 320
DpnII GATC 2 cut(s) 180, 318
DraIII CACNNNGTG 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 246
EarI CTCTTC 1 cut(s) 246
Ecl136II GAGCTC 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 72
Eco24I GRGCYC 1 cut(s) 117
Eco53kI GAGCTC 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 300
EcoICRI GAGCTC 1 cut(s) 115
EcoRII CCWGG 4 cut(s) 52, 99, 299, 313
EcoT14I CCWWGG 1 cut(s) 72
EcoT22I ATGCAT 1 cut(s) 332
EcoT38I GRGCYC 1 cut(s) 117
ErhI CCWWGG 1 cut(s) 72
FaeI CATG 4 cut(s) 76, 299, 330, 334
FaiI YATR 7 cut(s) 47, 74, 261, 297, 311, 328, 332
FaqI GGGAC 1 cut(s) 289
FatI CATG 4 cut(s) 72, 295, 326, 330
FbaI TGATCA 1 cut(s) 180
Fnu4HI GCNGC 2 cut(s) 23, 229
FriOI GRGCYC 1 cut(s) 117
Fsp4HI GCNGC 2 cut(s) 23, 229
GluI GCNGC 2 cut(s) 23, 229
GsuI CTGGAG 1 cut(s) 75
HaeIII GGCC 1 cut(s) 38
Hin1II CATG 4 cut(s) 76, 299, 330, 334
HpyAV CCTTC 2 cut(s) 130, 275
HpyCH4III ACNGT 2 cut(s) 34, 178
HpyCH4V TGCA 3 cut(s) 107, 228, 330
HpyF10VI GCNNNNNNNGC 1 cut(s) 83
HpyF3I CTNAG 2 cut(s) 78, 202
Hsp92II CATG 4 cut(s) 76, 299, 330, 334
Ksp22I TGATCA 1 cut(s) 180
Kzo9I GATC 2 cut(s) 180, 318
LpnPI CCDG 9 cut(s) 39, 66, 86, 113, 150, 286, 300, 313, 327
Lsp1109I GCAGC 2 cut(s) 34, 240
LweI GCATC 1 cut(s) 253
MabI ACCWGGT 1 cut(s) 99
MaeIII GTNAC 2 cut(s) 187, 248
MalI GATC 2 cut(s) 182, 320
MboI GATC 2 cut(s) 180, 318
MboII GAAGA 2 cut(s) 115, 233
MhlI GDGCHC 3 cut(s) 68, 117, 174
MluCI AATT 2 cut(s) 143, 155
MnlI CCTC 3 cut(s) 49, 50, 233
Mph1103I ATGCAT 1 cut(s) 332
MroXI GAANNNNTTC 1 cut(s) 285
MslI CAYNNNNRTG 1 cut(s) 71
MspR9I CCNGG 4 cut(s) 54, 101, 301, 315
MvaI CCWGG 4 cut(s) 54, 101, 301, 315
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 1 cut(s) 83
NcoI CCATGG 1 cut(s) 72
NdeII GATC 2 cut(s) 180, 318
NlaIII CATG 4 cut(s) 76, 299, 330, 334
NlaIV GGNNCC 1 cut(s) 135
NsiI ATGCAT 1 cut(s) 332
NspI RCATGY 3 cut(s) 299, 330, 334
NspV TTCGAA 1 cut(s) 147
PaeI GCATGC 1 cut(s) 334
PdmI GAANNNNTTC 1 cut(s) 285
PflMI CCANNNNNTGG 1 cut(s) 100
PkrI GCNGC 2 cut(s) 24, 230
PsiI TTATAA 1 cut(s) 47
Psp124BI GAGCTC 1 cut(s) 117
Psp6I CCWGG 4 cut(s) 52, 99, 299, 313
PspGI CCWGG 4 cut(s) 52, 99, 299, 313
PspN4I GGNNCC 1 cut(s) 135
PstI CTGCAG 1 cut(s) 230
RsaI GTAC 2 cut(s) 92, 325
RsaNI GTAC 2 cut(s) 91, 324
RseI CAYNNNNRTG 1 cut(s) 71
SacI GAGCTC 1 cut(s) 117
SatI GCNGC 2 cut(s) 23, 229
Sau3AI GATC 2 cut(s) 180, 318
ScrFI CCNGG 4 cut(s) 54, 101, 301, 315
SduI GDGCHC 3 cut(s) 68, 117, 174
SetI ASST 6 cut(s) 27, 84, 102, 117, 233, 286
SexAI ACCWGGT 1 cut(s) 99
SfaNI GCATC 1 cut(s) 253
SfcI CTRYAG 1 cut(s) 226
SfuI TTCGAA 1 cut(s) 147
SmiMI CAYNNNNRTG 1 cut(s) 71
SphI GCATGC 1 cut(s) 334
Sse9I AATT 2 cut(s) 143, 155
SsiI CCGC 1 cut(s) 166
SstI GAGCTC 1 cut(s) 117
StyD4I CCNGG 4 cut(s) 52, 99, 299, 313
StyI CCWWGG 1 cut(s) 72
TaaI ACNGT 2 cut(s) 34, 178
TaqI TCGA 1 cut(s) 147
TasI AATT 2 cut(s) 143, 155
TatI WGTACW 1 cut(s) 323
TseI GCWGC 2 cut(s) 22, 228
Van91I CCANNNNNTGG 1 cut(s) 100
XceI RCATGY 3 cut(s) 299, 330, 334
XmnI GAANNNNTTC 1 cut(s) 285
Zsp2I ATGCAT 1 cut(s) 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.