AT1G67270

subgroup (InterPro IPR018500), Cell division cycle-associated protein (InterPro IPR018866)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000989
Physical Location & Seq
Reverse (-)
25183019 .. 25185939
2921 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G67270.1

Sequence Viewer

Length: 1482 bp
ATGGTGAGAAAGCGTGAAACCAATCCCGGTGTTAGAGTTGTCGGAAAAAGAATTTATGACTCCCAAAATGGCAAATCATGTCACCAGTGTCGACAGAAGACTCTTGACTTTGCTGCACCGTGCAAGGCAATGAGGAGGAAGAAGCTATGTCCCATAAAGTTTTGTTACAAATGCTTATCAATCAGGTATGGTGAAAATGCAGAAGAAGTAGCAAAGTTGGATGATTGGATATGTCCTCTGTGCAGAGGCATCTGCATCTGTAGTGTATGCAGAAAAGCCCAAGGACTAGAGCCCACTGGGATTTTGGCACACGAAGCTAAAGCCCGTGGATATTCCTCAGTCTATGCGCTTCTTGAAGCTGAGGGGCATGATAAGTTTGCTTATCAGAAGAAACCAGAACTGGTTTCTGCTTGCAATGAAGGTAGTAGTAGCAATAGTGAATCTGTTCCAGGGACAGAAGTGGGAATATTAGATGTTCCCAAGGAGAAGAAGAAAGTGGTGGACAAGAGGAAGAGAGCCAAACCCAGTGACAAACTGAAAGAGGAGATTCAGCTTGAGGCTCAACTTCCTGAGGGTATAAGCTTGACTTGTGTTTCACGCATTGATATACCAACTGAAGAAGCTGGAAATGTATGTCAGCTTTTCGAGTTTTGCTCAGCCTTTGGAAAGGCACTTGCTTTGAAGGAAGGACATGCTGAAACTATTGTCCGTGAGCTGTTTATATGTGGGCGTAACACAAGGAGACAACAATACTGCTCTATTACTCAGATGATGATCCAGTTGTTGGATTTAATATCAAAGGATAGAGAGATGTCTTTGTCTCTAAGTGCAACGGACAGCAGTTGGTTCACGGCTATAGGAGAGATCCTATCGAAGTCAGAAGTTTTGAGTGATGAGCTCCCACTAGAAACTTTTGTAGGGGGTGTTGCTGCGTATGAGAAGATGAATGCATCAAGAAAGCTCAAGCTTCTAAATTTCTTATGTGATGAATCACTTAGCACATGGGCAATGAGGAATTACATAAATAGCCAAACTGAAGAGTGTGGAGAACGGAAAAAGGAAGCTAAACAAAAGGCTGCAGCCGCAAAGCAAAAGGAGAAGCAGCTCAAACAGAAGATGCAAGGTGAGGTTGCCAAATCCATTATGGAGAAAAACGGAGCTCCATTGTCTATTGAGGAACACAACTCAATCGTGTCTCAAATAAGAGATGAAGCTAAAGAAGCTCATGAAGAAATGATGGAGGCAAAGGGCATGAAATCTAAAATGACGCTTATATGTGATGCGCGTAGAACGGAACCAATCATGTTGGATGATAATGGTTTTGTTCTCTGGAAATTGAAGTGTTTTGAAGAAGAACAATTTTTTTTGCTTCAAGATCTAGGAACATTTGATGATTTATGTCCACATGAAAGATGGTTAGCTTTTAAACCTGAGCAGAAACCAGAGATAGAGAATATATCTCTTGTAAGAGGAGGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
WHIM1 zf-4CXXC_R1

Protein Analysis

493

Amino Acids

55.58

Weight (kDa)

8.09

Isoelectric Point (pI)

42.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 19 - 118 8.8e-36 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 784
AccI GTMKAC 1 cut(s) 91
AccII CGCG 1 cut(s) 1285
AciI CCGC 1 cut(s) 1083
AclWI GGATC 2 cut(s) 769, 859
AcsI RAATTY 2 cut(s) 51, 973
AcuI CTGAAG 2 cut(s) 636, 1056
AfiI CCNNNNNNNGG 1 cut(s) 784
AgsI TTSAA 5 cut(s) 356, 682, 1339, 1349, 1373
AjnI CCWGG 1 cut(s) 448
Alw21I GWGCWC 2 cut(s) 900, 1162
Alw26I GTCTC 3 cut(s) 736, 825, 1200
AlwI GGATC 2 cut(s) 769, 859
ApeKI GCWGC 5 cut(s) 113, 929, 1076, 1079, 1102
ApoI RAATTY 2 cut(s) 51, 973
Asp700I GAANNNNTTC 1 cut(s) 444
AspLEI GCGC 2 cut(s) 349, 1285
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 4 cut(s) 16, 74, 203, 1136
AxyI CCTNAGG 1 cut(s) 570
BanII GRGCYC 3 cut(s) 294, 900, 1162
BbsI GAAGAC 1 cut(s) 104
Bbv12I GWGCWC 2 cut(s) 900, 1162
BbvCI CCTCAGC 1 cut(s) 360
BbvI GCAGC 5 cut(s) 100, 916, 1063, 1091, 1114
BccI CCATC 2 cut(s) 1231, 1407
BceAI ACGGC 1 cut(s) 867
BciT130I CCWGG 1 cut(s) 450
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 3 cut(s) 736, 825, 1200
BfaI CTAG 3 cut(s) 287, 905, 1379
BfmI CTRYAG 3 cut(s) 259, 855, 1077
BglII AGATCT 1 cut(s) 1375
BisI GCNGC 6 cut(s) 114, 930, 1077, 1080, 1083, 1103
BlpI GCTNAGC 1 cut(s) 655
BlsI GCNGC 6 cut(s) 115, 931, 1078, 1081, 1084, 1104
Bme1390I CCNGG 2 cut(s) 27, 450
BmiI GGNNCC 1 cut(s) 1296
BmrFI CCNGG 2 cut(s) 27, 450
BmrI ACTGGG 2 cut(s) 306, 519
BmsI GCATC 5 cut(s) 258, 264, 959, 1107, 1270
BmuI ACTGGG 2 cut(s) 306, 519
BpiI GAAGAC 1 cut(s) 104
BplI GAGNNNNNCTC 2 cut(s) 638, 670
Bpu10I CCTNAGC 2 cut(s) 360, 1431
Bpu1102I GCTNAGC 1 cut(s) 655
BpuEI CTTGAG 2 cut(s) 575, 947
BpuMI CCSGG 1 cut(s) 27
BsaBI GATNNNNATC 1 cut(s) 773
BsaJI CCNNGG 4 cut(s) 280, 325, 449, 480
Bsc4I CCNNNNNNNGG 1 cut(s) 784
Bse1I ACTGG 5 cut(s) 85, 301, 405, 525, 778
Bse21I CCTNAGG 1 cut(s) 570
Bse3DI GCAATG 3 cut(s) 135, 421, 1014
Bse8I GATNNNNATC 1 cut(s) 773
BseBI CCWGG 1 cut(s) 450
BseDI CCNNGG 4 cut(s) 280, 325, 449, 480
BseGI GGATG 2 cut(s) 226, 1315
BseJI GATNNNNATC 1 cut(s) 773
BseLI CCNNNNNNNGG 1 cut(s) 784
BseMI GCAATG 3 cut(s) 135, 421, 1014
BseMII CTCAG 6 cut(s) 351, 351, 561, 669, 779, 1422
BseNI ACTGG 5 cut(s) 85, 301, 405, 525, 778
BseRI GAGGAG 2 cut(s) 148, 557
BseXI GCAGC 5 cut(s) 100, 916, 1063, 1091, 1114
BsgI GTGCAG 2 cut(s) 99, 262
Bsh1236I CGCG 1 cut(s) 1285
BsiHKAI GWGCWC 2 cut(s) 900, 1162
BsiSI CCGG 1 cut(s) 27
BslFI GGGAC 2 cut(s) 135, 466
BslI CCNNNNNNNGG 1 cut(s) 784
BsmAI GTCTC 3 cut(s) 736, 825, 1200
BsmFI GGGAC 2 cut(s) 135, 466
BsmI GAATGC 1 cut(s) 952
Bsp1286I GDGCHC 3 cut(s) 294, 900, 1162
Bsp143I GATC 3 cut(s) 774, 864, 1375
Bsp1720I GCTNAGC 1 cut(s) 655
BspACI CCGC 1 cut(s) 1083
BspCNI CTCAG 6 cut(s) 350, 352, 562, 668, 778, 1423
BspFNI CGCG 1 cut(s) 1285
BspHI TCATGA 1 cut(s) 1225
BspLI GGNNCC 1 cut(s) 1296
BspMAI CTGCAG 1 cut(s) 1081
BspPI GGATC 2 cut(s) 769, 859
BsrDI GCAATG 3 cut(s) 135, 421, 1014
BsrI ACTGG 5 cut(s) 85, 301, 405, 525, 778
BssECI CCNNGG 4 cut(s) 280, 325, 449, 480
BssMI GATC 3 cut(s) 774, 864, 1375
BssT1I CCWWGG 2 cut(s) 280, 480
Bst2UI CCWGG 1 cut(s) 450
Bst4CI ACNGT 1 cut(s) 120
Bst6I CTCTTC 2 cut(s) 506, 1032
BstC8I GCNNGC 1 cut(s) 412
BstDEI CTNAG 8 cut(s) 337, 360, 570, 655, 765, 824, 995, 1431
BstDSI CCRYGG 1 cut(s) 325
BstF5I GGATG 2 cut(s) 226, 1315
BstFNI CGCG 1 cut(s) 1285
BstHHI GCGC 2 cut(s) 349, 1285
BstKTI GATC 3 cut(s) 777, 867, 1378
BstMAI GTCTC 3 cut(s) 736, 825, 1200
BstMBI GATC 3 cut(s) 774, 864, 1375
BstMWI GCNNNNNNNGC 3 cut(s) 314, 1082, 1220
BstNI CCWGG 1 cut(s) 450
BstNSI RCATGY 1 cut(s) 695
BstSCI CCNGG 2 cut(s) 25, 448
BstSFI CTRYAG 3 cut(s) 259, 855, 1077
BstUI CGCG 1 cut(s) 1285
BstV1I GCAGC 5 cut(s) 100, 916, 1063, 1091, 1114
BstV2I GAAGAC 1 cut(s) 104
BstX2I RGATCY 2 cut(s) 864, 1375
BstYI RGATCY 2 cut(s) 864, 1375
Bsu36I CCTNAGG 1 cut(s) 570
BtgI CCRYGG 1 cut(s) 325
BtsCI GGATG 2 cut(s) 226, 1315
BtsIMutI CAGTG 3 cut(s) 92, 294, 532
Cac8I GCNNGC 1 cut(s) 412
CciI TCATGA 1 cut(s) 1225
CfoI GCGC 2 cut(s) 349, 1285
CseI GACGC 1 cut(s) 1276
CviAII CATG 8 cut(s) 78, 368, 692, 1002, 1226, 1252, 1303, 1406
DdeI CTNAG 8 cut(s) 337, 360, 570, 655, 765, 824, 995, 1431
DpnI GATC 3 cut(s) 776, 866, 1377
DpnII GATC 3 cut(s) 774, 864, 1375
DraI TTTAAA 1 cut(s) 1426
Eam1104I CTCTTC 2 cut(s) 506, 1032
EarI CTCTTC 2 cut(s) 506, 1032
Ecl136II GAGCTC 2 cut(s) 898, 1160
Eco130I CCWWGG 2 cut(s) 280, 480
Eco24I GRGCYC 3 cut(s) 294, 900, 1162
Eco53kI GAGCTC 2 cut(s) 898, 1160
Eco57I CTGAAG 2 cut(s) 636, 1056
Eco81I CCTNAGG 1 cut(s) 570
EcoICRI GAGCTC 2 cut(s) 898, 1160
EcoRII CCWGG 1 cut(s) 448
EcoT14I CCWWGG 2 cut(s) 280, 480
EcoT22I ATGCAT 1 cut(s) 952
EcoT38I GRGCYC 3 cut(s) 294, 900, 1162
ErhI CCWWGG 2 cut(s) 280, 480
FaeI CATG 8 cut(s) 81, 371, 695, 1005, 1229, 1255, 1306, 1409
FalI AAGNNNNNCTT 2 cut(s) 571, 603
FaqI GGGAC 2 cut(s) 135, 466
FatI CATG 8 cut(s) 77, 367, 691, 1001, 1225, 1251, 1302, 1405
FblI GTMKAC 1 cut(s) 91
Fnu4HI GCNGC 6 cut(s) 114, 930, 1077, 1080, 1083, 1103
FokI GGATG 2 cut(s) 233, 1322
FriOI GRGCYC 3 cut(s) 294, 900, 1162
Fsp4HI GCNGC 6 cut(s) 114, 930, 1077, 1080, 1083, 1103
FspBI CTAG 3 cut(s) 287, 905, 1379
GlaI GCGC 2 cut(s) 348, 1284
GluI GCNGC 6 cut(s) 114, 930, 1077, 1080, 1083, 1103
HapII CCGG 1 cut(s) 27
HgaI GACGC 1 cut(s) 1276
HhaI GCGC 2 cut(s) 349, 1285
Hin1II CATG 8 cut(s) 81, 371, 695, 1005, 1229, 1255, 1306, 1409
Hin6I GCGC 2 cut(s) 347, 1283
HinP1I GCGC 2 cut(s) 347, 1283
HincII GTYRAC 1 cut(s) 92
HindII GTYRAC 1 cut(s) 92
HindIII AAGCTT 2 cut(s) 580, 965
HinfI GANTC 5 cut(s) 59, 100, 440, 547, 989
HpaII CCGG 1 cut(s) 27
HphI GGTGA 4 cut(s) 16, 74, 203, 1136
Hpy166II GTNNAC 4 cut(s) 92, 502, 849, 1403
Hpy188I TCNGA 4 cut(s) 44, 387, 768, 880
Hpy188III TCNNGA 7 cut(s) 104, 353, 569, 954, 1226, 1330, 1373
Hpy8I GTNNAC 4 cut(s) 92, 502, 849, 1403
HpyAV CCTTC 3 cut(s) 413, 676, 680
HpyCH4III ACNGT 1 cut(s) 120
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 1082, 1220
HpyF3I CTNAG 8 cut(s) 337, 360, 570, 655, 765, 824, 995, 1431
Hsp92II CATG 8 cut(s) 81, 371, 695, 1005, 1229, 1255, 1306, 1409
HspAI GCGC 2 cut(s) 347, 1283
Kzo9I GATC 3 cut(s) 774, 864, 1375
LmnI GCTCC 3 cut(s) 903, 1157, 1165
Lsp1109I GCAGC 5 cut(s) 100, 916, 1063, 1091, 1114
LweI GCATC 5 cut(s) 258, 264, 959, 1107, 1270
MaeI CTAG 3 cut(s) 287, 905, 1379
MaeIII GTNAC 4 cut(s) 80, 164, 527, 731
MalI GATC 3 cut(s) 776, 866, 1377
MboI GATC 3 cut(s) 774, 864, 1375
MflI RGATCY 2 cut(s) 864, 1375
MhlI GDGCHC 3 cut(s) 294, 900, 1162
MluCI AATT 5 cut(s) 51, 973, 1015, 1334, 1358
MlyI GAGTC 2 cut(s) 53, 94
MmeI TCCRAC 4 cut(s) 22, 198, 765, 1287
Mph1103I ATGCAT 1 cut(s) 952
MroXI GAANNNNTTC 1 cut(s) 444
MseI TTAA 2 cut(s) 791, 1425
MspI CCGG 1 cut(s) 27
MspR9I CCNGG 2 cut(s) 27, 450
Mva1269I GAATGC 1 cut(s) 952
MvaI CCWGG 1 cut(s) 450
MvnI CGCG 1 cut(s) 1285
MwoI GCNNNNNNNGC 3 cut(s) 314, 1082, 1220
NciI CCSGG 1 cut(s) 27
NdeII GATC 3 cut(s) 774, 864, 1375
NlaIII CATG 8 cut(s) 81, 371, 695, 1005, 1229, 1255, 1306, 1409
NlaIV GGNNCC 1 cut(s) 1296
NmuCI GTSAC 2 cut(s) 80, 527
NsiI ATGCAT 1 cut(s) 952
NspI RCATGY 1 cut(s) 695
PagI TCATGA 1 cut(s) 1225
PctI GAATGC 1 cut(s) 952
PdmI GAANNNNTTC 1 cut(s) 444
PfeI GAWTC 3 cut(s) 440, 547, 989
PflMI CCANNNNNTGG 1 cut(s) 784
PkrI GCNGC 6 cut(s) 115, 931, 1078, 1081, 1084, 1104
PleI GAGTC 2 cut(s) 53, 94
PpsI GAGTC 2 cut(s) 53, 94
Psp124BI GAGCTC 2 cut(s) 900, 1162
Psp6I CCWGG 1 cut(s) 448
PspGI CCWGG 1 cut(s) 448
PspN4I GGNNCC 1 cut(s) 1296
PstI CTGCAG 1 cut(s) 1081
PsuI RGATCY 2 cut(s) 864, 1375
SacI GAGCTC 2 cut(s) 900, 1162
SalI GTCGAC 1 cut(s) 90
SaqAI TTAA 2 cut(s) 791, 1425
SatI GCNGC 6 cut(s) 114, 930, 1077, 1080, 1083, 1103
Sau3AI GATC 3 cut(s) 774, 864, 1375
SchI GAGTC 2 cut(s) 53, 94
ScrFI CCNGG 2 cut(s) 27, 450
SduI GDGCHC 3 cut(s) 294, 900, 1162
SfaNI GCATC 5 cut(s) 258, 264, 959, 1107, 1270
SfcI CTRYAG 3 cut(s) 259, 855, 1077
SmlI CTYRAG 2 cut(s) 554, 962
SmoI CTYRAG 2 cut(s) 554, 962
Sse9I AATT 5 cut(s) 51, 973, 1015, 1334, 1358
SsiI CCGC 1 cut(s) 1083
SspI AATATT 1 cut(s) 468
SspMI CTAG 3 cut(s) 287, 905, 1379
SstI GAGCTC 2 cut(s) 900, 1162
StyD4I CCNGG 2 cut(s) 25, 448
StyI CCWWGG 2 cut(s) 280, 480
TaaI ACNGT 1 cut(s) 120
TaqI TCGA 3 cut(s) 91, 645, 872
TasI AATT 5 cut(s) 51, 973, 1015, 1334, 1358
TauI GCSGC 1 cut(s) 1085
TfiI GAWTC 3 cut(s) 440, 547, 989
Tru1I TTAA 2 cut(s) 791, 1425
Tru9I TTAA 2 cut(s) 791, 1425
TscAI CASTG 3 cut(s) 92, 301, 532
TseFI GTSAC 2 cut(s) 80, 527
TseI GCWGC 5 cut(s) 113, 929, 1076, 1079, 1102
Tsp45I GTSAC 2 cut(s) 80, 527
TspDTI ATGAA 7 cut(s) 432, 959, 1002, 1224, 1242, 1268, 1422
TspGWI ACGGA 5 cut(s) 698, 848, 1066, 1170, 1307
TspRI CASTG 3 cut(s) 92, 301, 532
Van91I CCANNNNNTGG 1 cut(s) 784
XapI RAATTY 2 cut(s) 51, 973
XceI RCATGY 1 cut(s) 695
XcmI CCANNNNNNNNNTGG 3 cut(s) 301, 1141, 1410
XmiI GTMKAC 1 cut(s) 91
XmnI GAANNNNTTC 1 cut(s) 444
XspI CTAG 3 cut(s) 287, 905, 1379
Zsp2I ATGCAT 1 cut(s) 952
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.