RchiOBHm_Chr4g0409761

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000989
Physical Location & Seq
Forward (+)
33404197 .. 33406787
2591 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38074

Sequence Viewer

Length: 609 bp
ATGACGCGGTCAACGGAGCTCATCACCGGTGAAGACCCCGATGAAGGTCCCGAAGTCGCAACTAAAGATTTCAGTAAACTGAGGAGCTGGATAGAAGAACAGCATGAAAAGATTGTTGAAAGCGAAAAGGAAGCAAAAGGAAAAGTTAATGCAACCAAAGATAAGGAAAAACTCCTCAAGCGGAAGTTGCAAGATAAGGTGGCGAAGCTTATTATTGCAAAAAATGGTGCTCCTCTGTCAATTTCAGAACATGAAGCTCTTGTTTCACAAATAAACAGTGAAGCAGCTCAAGCTCACGCTGAGTTGCTTGAAGCAGAGGGCATAGTGCCTAAAAAGAAACATAGATGTGATGTTTTAAGAACAGAGCCTTATCGTGTGGATGTTGATGGCCGCATCTTTTGGAAATTAAAGGGTTACAACAATGGAGAAGATATCGTGCTTCAAGATTTGGGGGCCTGGGATGCAGTTGTATCTAAAGAAGAATGGTTTGTTTATGGTGTGGAGACAAAGGAAGTAATTGACAATTACTGTTCTTATTTAAGGAGAAAAAAGTCTAGTGGAACCGTGTCGCAAACTGTTCCTCGTGAAAGCGATGAAGAAAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

202

Amino Acids

22.93

Weight (kDa)

5.44

Isoelectric Point (pI)

33.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 7
AciI CCGC 3 cut(s) 7, 181, 391
AcoI YGGCCR 1 cut(s) 388
AfiI CCNNNNNNNGG 1 cut(s) 44
AflIII ACRYGT 1 cut(s) 603
AgeI ACCGGT 1 cut(s) 26
AgsI TTSAA 3 cut(s) 119, 311, 443
AjnI CCWGG 1 cut(s) 455
AluBI AGCT 6 cut(s) 19, 87, 208, 257, 287, 293
AluI AGCT 6 cut(s) 19, 87, 208, 257, 287, 293
Alw21I GWGCWC 2 cut(s) 21, 232
Alw26I GTCTC 1 cut(s) 497
AoxI GGCC 2 cut(s) 388, 453
ApeKI GCWGC 1 cut(s) 284
AsiGI ACCGGT 1 cut(s) 26
AspS9I GGNCC 2 cut(s) 47, 453
AsuHPI GGTGA 2 cut(s) 16, 41
AvaII GGWCC 1 cut(s) 47
BanII GRGCYC 1 cut(s) 21
BauI CACGAG 1 cut(s) 582
BbsI GAAGAC 1 cut(s) 39
Bbv12I GWGCWC 2 cut(s) 21, 232
BbvI GCAGC 1 cut(s) 296
BccI CCATC 1 cut(s) 380
BciT130I CCWGG 1 cut(s) 457
BcoDI GTCTC 1 cut(s) 497
BfaI CTAG 1 cut(s) 555
BisI GCNGC 2 cut(s) 285, 391
BlsI GCNGC 2 cut(s) 286, 392
Bme1390I CCNGG 1 cut(s) 457
Bme18I GGWCC 1 cut(s) 47
BmgT120I GGNCC 2 cut(s) 47, 453
BmiI GGNNCC 3 cut(s) 49, 454, 562
BmrFI CCNGG 1 cut(s) 457
BmsI GCATC 2 cut(s) 402, 451
BpiI GAAGAC 1 cut(s) 39
BpuEI CTTGAG 2 cut(s) 161, 273
BsaJI CCNNGG 1 cut(s) 456
BsaWI WCCGGW 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 44
Bse118I RCCGGY 1 cut(s) 26
BseBI CCWGG 1 cut(s) 457
BseDI CCNNGG 1 cut(s) 456
BseGI GGATG 2 cut(s) 385, 466
BseLI CCNNNNNNNGG 1 cut(s) 44
BseMII CTCAG 2 cut(s) 71, 291
BseRI GAGGAG 3 cut(s) 97, 164, 222
BseXI GCAGC 1 cut(s) 296
Bsh1236I CGCG 1 cut(s) 7
BshFI GGCC 2 cut(s) 390, 455
BshTI ACCGGT 1 cut(s) 26
BsiHKAI GWGCWC 2 cut(s) 21, 232
BsiSI CCGG 1 cut(s) 27
BslFI GGGAC 1 cut(s) 33
BslI CCNNNNNNNGG 1 cut(s) 44
BsmAI GTCTC 1 cut(s) 497
BsmFI GGGAC 1 cut(s) 33
BsnI GGCC 2 cut(s) 390, 455
Bsp1286I GDGCHC 2 cut(s) 21, 232
BspACI CCGC 3 cut(s) 7, 181, 391
BspANI GGCC 2 cut(s) 390, 455
BspCNI CTCAG 2 cut(s) 72, 292
BspFNI CGCG 1 cut(s) 7
BspLI GGNNCC 3 cut(s) 49, 454, 562
BsrFI RCCGGY 1 cut(s) 26
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 1 cut(s) 456
BssSI CACGAG 1 cut(s) 582
Bst2BI CACGAG 1 cut(s) 582
Bst2UI CCWGG 1 cut(s) 457
Bst4CI ACNGT 4 cut(s) 278, 530, 565, 577
BstDEI CTNAG 2 cut(s) 80, 300
BstF5I GGATG 2 cut(s) 385, 466
BstFNI CGCG 1 cut(s) 7
BstMAI GTCTC 1 cut(s) 497
BstMWI GCNNNNNNNGC 3 cut(s) 187, 290, 461
BstNI CCWGG 1 cut(s) 457
BstNSI RCATGY 1 cut(s) 607
BstSCI CCNGG 1 cut(s) 455
BstUI CGCG 1 cut(s) 7
BstV1I GCAGC 1 cut(s) 296
BstV2I GAAGAC 1 cut(s) 39
BsuRI GGCC 2 cut(s) 390, 455
BtsCI GGATG 2 cut(s) 385, 466
BtsIMutI CAGTG 1 cut(s) 283
Cfr10I RCCGGY 1 cut(s) 26
Cfr13I GGNCC 2 cut(s) 47, 453
CseI GACGC 1 cut(s) 13
CspAI ACCGGT 1 cut(s) 26
CviAII CATG 3 cut(s) 104, 251, 604
CviJI RGCY 9 cut(s) 19, 87, 208, 257, 287, 293, 367, 390, 455
CviKI_1 RGCY 9 cut(s) 19, 87, 208, 257, 287, 293, 367, 390, 455
DdeI CTNAG 2 cut(s) 80, 300
EaeI YGGCCR 1 cut(s) 388
Ecl136II GAGCTC 1 cut(s) 19
Eco24I GRGCYC 1 cut(s) 21
Eco32I GATATC 1 cut(s) 433
Eco47I GGWCC 1 cut(s) 47
Eco53kI GAGCTC 1 cut(s) 19
EcoICRI GAGCTC 1 cut(s) 19
EcoO109I RGGNCCY 2 cut(s) 47, 453
EcoRII CCWGG 1 cut(s) 455
EcoRV GATATC 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 21
FaeI CATG 3 cut(s) 107, 254, 607
FaiI YATR 6 cut(s) 105, 252, 323, 342, 495, 605
FaqI GGGAC 1 cut(s) 33
FatI CATG 3 cut(s) 103, 250, 603
Fnu4HI GCNGC 2 cut(s) 285, 391
FokI GGATG 2 cut(s) 392, 473
FriOI GRGCYC 1 cut(s) 21
Fsp4HI GCNGC 2 cut(s) 285, 391
FspBI CTAG 1 cut(s) 555
GluI GCNGC 2 cut(s) 285, 391
HaeIII GGCC 2 cut(s) 390, 455
HapII CCGG 1 cut(s) 27
HgaI GACGC 1 cut(s) 13
Hin1II CATG 3 cut(s) 107, 254, 607
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HindIII AAGCTT 1 cut(s) 206
HpaII CCGG 1 cut(s) 27
HphI GGTGA 2 cut(s) 16, 41
Hpy166II GTNNAC 2 cut(s) 12, 77
Hpy188I TCNGA 1 cut(s) 247
Hpy188III TCNNGA 3 cut(s) 50, 443, 584
Hpy8I GTNNAC 2 cut(s) 12, 77
HpyAV CCTTC 1 cut(s) 38
HpyCH4III ACNGT 4 cut(s) 278, 530, 565, 577
HpyCH4V TGCA 4 cut(s) 152, 190, 218, 464
HpyF10VI GCNNNNNNNGC 3 cut(s) 187, 290, 461
HpyF3I CTNAG 2 cut(s) 80, 300
Hsp92II CATG 3 cut(s) 107, 254, 607
LmnI GCTCC 3 cut(s) 16, 84, 235
LpnPI CCDG 4 cut(s) 40, 73, 442, 469
Lsp1109I GCAGC 1 cut(s) 296
LweI GCATC 2 cut(s) 402, 451
MaeI CTAG 1 cut(s) 555
MaeIII GTNAC 1 cut(s) 413
MboII GAAGA 5 cut(s) 44, 107, 440, 491, 608
MhlI GDGCHC 2 cut(s) 21, 232
MluCI AATT 4 cut(s) 240, 404, 516, 523
MnlI CCTC 5 cut(s) 75, 185, 243, 310, 591
MseI TTAA 4 cut(s) 147, 356, 407, 539
MslI CAYNNNNRTG 1 cut(s) 345
MspI CCGG 1 cut(s) 27
MspR9I CCNGG 1 cut(s) 457
MvaI CCWGG 1 cut(s) 457
MvnI CGCG 1 cut(s) 7
MwoI GCNNNNNNNGC 3 cut(s) 187, 290, 461
NlaIII CATG 3 cut(s) 107, 254, 607
NlaIV GGNNCC 3 cut(s) 49, 454, 562
NspI RCATGY 1 cut(s) 607
PciI ACATGT 1 cut(s) 603
PflFI GACNNNGTC 1 cut(s) 7
PinAI ACCGGT 1 cut(s) 26
PkrI GCNGC 2 cut(s) 286, 392
PpuMI RGGWCCY 1 cut(s) 47
PscI ACATGT 1 cut(s) 603
Psp124BI GAGCTC 1 cut(s) 21
Psp5II RGGWCCY 1 cut(s) 47
Psp6I CCWGG 1 cut(s) 455
PspGI CCWGG 1 cut(s) 455
PspN4I GGNNCC 3 cut(s) 49, 454, 562
PspPI GGNCC 2 cut(s) 47, 453
PspPPI RGGWCCY 1 cut(s) 47
PsyI GACNNNGTC 1 cut(s) 7
RseI CAYNNNNRTG 1 cut(s) 345
SacI GAGCTC 1 cut(s) 21
SaqAI TTAA 4 cut(s) 147, 356, 407, 539
SatI GCNGC 2 cut(s) 285, 391
Sau96I GGNCC 2 cut(s) 47, 453
ScrFI CCNGG 1 cut(s) 457
SduI GDGCHC 2 cut(s) 21, 232
SetI ASST 8 cut(s) 21, 49, 89, 201, 210, 259, 289, 295
SfaNI GCATC 2 cut(s) 402, 451
SgrAI CRCCGGYG 1 cut(s) 26
SinI GGWCC 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 2 cut(s) 176, 288
SmoI CTYRAG 2 cut(s) 176, 288
Sse9I AATT 4 cut(s) 240, 404, 516, 523
SsiI CCGC 3 cut(s) 7, 181, 391
SspMI CTAG 1 cut(s) 555
SstI GAGCTC 1 cut(s) 21
StyD4I CCNGG 1 cut(s) 455
TaaI ACNGT 4 cut(s) 278, 530, 565, 577
TasI AATT 4 cut(s) 240, 404, 516, 523
TauI GCSGC 1 cut(s) 393
Tru1I TTAA 4 cut(s) 147, 356, 407, 539
Tru9I TTAA 4 cut(s) 147, 356, 407, 539
TscAI CASTG 1 cut(s) 283
TseI GCWGC 1 cut(s) 284
TspDTI ATGAA 4 cut(s) 57, 120, 267, 609
TspGWI ACGGA 1 cut(s) 29
TspRI CASTG 1 cut(s) 283
Tth111I GACNNNGTC 1 cut(s) 7
VpaK11BI GGWCC 1 cut(s) 47
XceI RCATGY 1 cut(s) 607
XspI CTAG 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.