pycom09g01070

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000989
Physical Location & Seq
Reverse (-)
856963 .. 860227
3265 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g01070.1

Sequence Viewer

Length: 1746 bp
ATGGTGGTGGAGGAGAGCAACAAGGTTGAGGATACTATGGTGGTCGGGAAACAACGCGTTCGGGCCGTCGGTGGCCAGACCTACGACTCCAAGAAAGGAGTTTCCTGCCATCAGTGCCGTCAGAAGATGACGGAAAAGTTGACAGCTTTGGCTGCCTCCTGTAAGAACGTCAAAGCGAAAGGCATTTGCCCGATTCAGTTTTGCCATAAGTGTCTGTGGAACAGGTATGGATTGAAAGCTGAACAAGTGGAACTCGAGGATGATTGGAAATGTCCGAAATGTCAAGGCAATTGCAATTGCAGTATATGCTTACTAGTGTGCTTTATCAGGAAGAAAAACGGTTACCAACCCACTGGTCTTCTCGTGTACGAAGCCAAGGCGAGAGGGTTTCAATCTGTTTCTGATATGCTGACTATGTACAAGCCAAAAAAGCAAGGGGATGAGGATGACAAAAGCAAGGATGGCTCACCACAAAAGCGCGTCGCTTCAGAGAAGGCAATTGTCGTTGGTTCACCAAGAAAACGAGGGAAGGAGAACACTCTCGATCGAAGCAAAGATATGAACTTGGATGCAGCTCTGAACTCTGGTGAGCAGGAAACTAAGAAAGCAAAGCGGGAAGAGTTGACAGAAGTTTGTAATGGCAGCAGGGATGGTGGTGCTACTGTGAGTAATAAGGATGCCAAGCCCAAGGCCAAGGAAGTCAGAAGGAACCGGAAAGTAAAGGAATGCCCTGTGAATATTGAGAATCAAAAGGTTGATCTTGACCTTCCCCTGCCACAGGGCAATCCCTTGACAACTGTAGCTGGTGTTGAATTGGCTGCTGAGGATGTTGGAAATGCACTGCAGTTTCTGAAATTTTGTGAAACTTTTGGAGAGTTTCTTAAAATCCAAAAATGCCAAGCTGAATCTGTACTTAGAGAACTATCACGTCGAGGAAGTGGGCGAAGGGGACGGCCTGGACAATACTCCTCAGTGATTCGATTTCATAACTATATGCTCTCCTTCATACAGAAGGGTTCCTTAGAGGAGTCTTCTTTAGATCAATCAAGCTACAATAAGTCCTGGTTCCAAGATTTGGGGGAACTTATCTCTGCATCCGGTGTCTCTAAGGTCGATCCTGTATTAAAGGAACTGTCCCCAGAGTGTTTTAGCAAAGGCGGGGATGGATATGATATGTTGAACATCACTCAAAAGCTCAGGATTTTGAATTTCCTTTGTGATGAGGCTCTTGACACCGAAGATCTCAAAAAGAAGTTGCAGAAGGAGATGACTAAGAGGATAATTGCAAACAATGGTTATCCTGTCCGAATTTCAGAACGTAACTCTGTTGTTTCACAAATCACAAGCAAGGCAGCTCAAGCTCACACTGAGGTGGTTGAAGCAATGGCCATGTCACCTAAAAGCAATGAAATATGTGAAGCTGTTAGAATAAAGGCTTATGTTTTGGATGTTCATGGCCGTGCATTTTGGAAACTGAACGGTGATTTTGGTGAAGATATTTTGCTTCAAGATATGGGAACATGGGATGGAGCTCCTTCTGTTGAAAACTGGTTCGTTTATGGCGCTGAAATGAAAGAGCCAATCGACAAATACCTCTCTTTTCTAAGTCTAAAACAAGAACGGGCCAGGAGCCAGAGGCCATTCAAAAGTCGGTTTGCAAGCAAAGAAGAAGACTTGCAGGTTCCTCAGACTCCTGAAAGCAATGAAGAAAACCCTCCGGTTTCCGACACCTTCTCAAAGCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

582

Amino Acids

65.12

Weight (kDa)

8.71

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 28 - 137 1.7e-28 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1669
AccB7I CCANNNNNTGG 1 cut(s) 1075
AccII CGCG 2 cut(s) 57, 480
AciI CCGC 2 cut(s) 613, 1158
AclWI GGATC 1 cut(s) 1109
AcoI YGGCCR 3 cut(s) 73, 1386, 1456
AcsI RAATTY 3 cut(s) 854, 1207, 1308
AcuI CTGAAG 1 cut(s) 471
AfaI GTAC 3 cut(s) 368, 419, 912
AfiI CCNNNNNNNGG 2 cut(s) 778, 1075
AflIII ACRYGT 1 cut(s) 55
AgsI TTSAA 9 cut(s) 235, 392, 812, 1180, 1207, 1379, 1508, 1544, 1645
AhlI ACTAGT 1 cut(s) 313
AjiI CACGTC 1 cut(s) 929
AjnI CCWGG 3 cut(s) 955, 1061, 1625
AleI CACNNNNGTG 1 cut(s) 1370
Alw21I GWGCWC 1 cut(s) 1534
Alw26I GTCTC 1 cut(s) 1108
AlwI GGATC 1 cut(s) 1109
AlwNI CAGNNNCTG 1 cut(s) 850
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 8 cut(s) 63, 73, 690, 953, 1386, 1456, 1623, 1637
ApeKI GCWGC 5 cut(s) 152, 572, 642, 818, 1352
ApoI RAATTY 3 cut(s) 854, 1207, 1308
AspLEI GCGC 2 cut(s) 480, 1565
AspS9I GGNCC 2 cut(s) 63, 1623
AsuHPI GGTGA 6 cut(s) 459, 504, 599, 1386, 1493, 1502
AvaI CYCGRG 1 cut(s) 254
BaeI ACNNNNGTAYC 2 cut(s) 24, 57
BalI TGGCCA 2 cut(s) 75, 1388
BanII GRGCYC 1 cut(s) 1534
BauI CACGAG 1 cut(s) 362
BbsI GAAGAC 3 cut(s) 350, 1023, 1677
Bbv12I GWGCWC 1 cut(s) 1534
BbvCI CCTCAGC 1 cut(s) 822
BbvI GCAGC 5 cut(s) 139, 584, 654, 805, 1364
BccI CCATC 5 cut(s) 117, 455, 644, 1157, 1520
BceAI ACGGC 4 cut(s) 50, 102, 968, 1443
BciT130I CCWGG 3 cut(s) 957, 1063, 1627
BciVI GTATCC 1 cut(s) 25
BcoDI GTCTC 1 cut(s) 1108
BcuI ACTAGT 1 cut(s) 313
BfaI CTAG 1 cut(s) 314
BfmI CTRYAG 2 cut(s) 798, 842
BfoI RGCGCY 1 cut(s) 1566
BfuAI ACCTGC 1 cut(s) 1669
BfuI GTATCC 1 cut(s) 25
BglII AGATCT 1 cut(s) 1240
BisI GCNGC 5 cut(s) 153, 573, 643, 819, 1353
BlsI GCNGC 5 cut(s) 154, 574, 644, 820, 1354
Bme1390I CCNGG 3 cut(s) 957, 1063, 1627
BmeT110I CYCGRG 1 cut(s) 254
BmgBI CACGTC 1 cut(s) 929
BmgT120I GGNCC 2 cut(s) 63, 1623
BmiI GGNNCC 5 cut(s) 710, 1018, 1067, 1631, 1683
BmrFI CCNGG 3 cut(s) 957, 1063, 1627
BmsI GCATC 3 cut(s) 559, 667, 1103
BpiI GAAGAC 3 cut(s) 350, 1023, 1677
Bpu10I CCTNAGC 2 cut(s) 822, 1196
BpuEI CTTGAG 1 cut(s) 1341
BsaJI CCNNGG 3 cut(s) 375, 687, 693
BsaWI WCCGGW 3 cut(s) 711, 1097, 1717
Bsc4I CCNNNNNNNGG 2 cut(s) 778, 1075
Bse1I ACTGG 2 cut(s) 358, 1553
Bse3DI GCAATG 3 cut(s) 1389, 1411, 1708
BseBI CCWGG 3 cut(s) 957, 1063, 1627
BseDI CCNNGG 3 cut(s) 375, 687, 693
BseLI CCNNNNNNNGG 2 cut(s) 778, 1075
BseMI GCAATG 3 cut(s) 1389, 1411, 1708
BseMII CTCAG 5 cut(s) 813, 984, 1210, 1359, 1700
BseNI ACTGG 2 cut(s) 358, 1553
BseRI GAGGAG 3 cut(s) 26, 958, 1040
BseXI GCAGC 5 cut(s) 139, 584, 654, 805, 1364
Bsh1236I CGCG 2 cut(s) 57, 480
Bsh1285I CGRYCG 1 cut(s) 547
BshFI GGCC 8 cut(s) 65, 75, 692, 955, 1388, 1458, 1625, 1639
BsiEI CGRYCG 1 cut(s) 547
BsiHKAI GWGCWC 1 cut(s) 1534
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 3 cut(s) 712, 1098, 1718
BslFI GGGAC 2 cut(s) 963, 1120
BslI CCNNNNNNNGG 2 cut(s) 778, 1075
BsmAI GTCTC 1 cut(s) 1108
BsmFI GGGAC 2 cut(s) 963, 1120
BsmI GAATGC 1 cut(s) 731
BsnI GGCC 8 cut(s) 65, 75, 692, 955, 1388, 1458, 1625, 1639
BsoBI CYCGRG 1 cut(s) 254
Bsp1286I GDGCHC 1 cut(s) 1534
Bsp1407I TGTACA 1 cut(s) 417
Bsp143I GATC 5 cut(s) 544, 757, 1039, 1114, 1240
BspACI CCGC 2 cut(s) 613, 1158
BspANI GGCC 8 cut(s) 65, 75, 692, 955, 1388, 1458, 1625, 1639
BspCNI CTCAG 5 cut(s) 814, 983, 1209, 1360, 1699
BspFNI CGCG 2 cut(s) 57, 480
BspLI GGNNCC 5 cut(s) 710, 1018, 1067, 1631, 1683
BspMAI CTGCAG 1 cut(s) 846
BspMI ACCTGC 1 cut(s) 1669
BspPI GGATC 1 cut(s) 1109
BsrDI GCAATG 3 cut(s) 1389, 1411, 1708
BsrGI TGTACA 1 cut(s) 417
BsrI ACTGG 2 cut(s) 358, 1553
BssECI CCNNGG 3 cut(s) 375, 687, 693
BssMI GATC 5 cut(s) 544, 757, 1039, 1114, 1240
BssSI CACGAG 1 cut(s) 362
BssT1I CCWWGG 3 cut(s) 375, 687, 693
Bst2BI CACGAG 1 cut(s) 362
Bst2UI CCWGG 3 cut(s) 957, 1063, 1627
Bst4CI ACNGT 5 cut(s) 341, 664, 799, 1134, 1481
Bst6I CTCTTC 1 cut(s) 612
BstAPI GCANNNNNTGC 1 cut(s) 306
BstAUI TGTACA 1 cut(s) 417
BstC8I GCNNGC 1 cut(s) 1660
BstEII GGTNACC 1 cut(s) 341
BstENI CCTNNNNNAGG 1 cut(s) 776
BstFNI CGCG 2 cut(s) 57, 480
BstH2I RGCGCY 1 cut(s) 1566
BstHHI GCGC 2 cut(s) 480, 1565
BstKTI GATC 5 cut(s) 547, 760, 1042, 1117, 1243
BstMAI GTCTC 1 cut(s) 1108
BstMBI GATC 5 cut(s) 544, 757, 1039, 1114, 1240
BstMCI CGRYCG 1 cut(s) 547
BstMWI GCNNNNNNNGC 6 cut(s) 114, 152, 306, 430, 462, 1358
BstNI CCWGG 3 cut(s) 957, 1063, 1627
BstPI GGTNACC 1 cut(s) 341
BstSCI CCNGG 3 cut(s) 955, 1061, 1625
BstSFI CTRYAG 2 cut(s) 798, 842
BstUI CGCG 2 cut(s) 57, 480
BstV1I GCAGC 5 cut(s) 139, 584, 654, 805, 1364
BstV2I GAAGAC 3 cut(s) 350, 1023, 1677
BstX2I RGATCY 1 cut(s) 1240
BstXI CCANNNNNNTGG 1 cut(s) 353
BstYI RGATCY 1 cut(s) 1240
BsuI GTATCC 1 cut(s) 25
BsuRI GGCC 8 cut(s) 65, 75, 692, 955, 1388, 1458, 1625, 1639
BtrI CACGTC 1 cut(s) 929
BtsI GCAGTG 1 cut(s) 839
BtsIMutI CAGTG 5 cut(s) 119, 351, 839, 978, 1365
BveI ACCTGC 1 cut(s) 1669
Cac8I GCNNGC 1 cut(s) 1660
CaiI CAGNNNCTG 1 cut(s) 850
CfoI GCGC 2 cut(s) 480, 1565
Cfr13I GGNCC 2 cut(s) 63, 1623
CseI GACGC 1 cut(s) 469
Csp6I GTAC 3 cut(s) 367, 418, 911
CviAII CATG 3 cut(s) 1390, 1454, 1521
CviQI GTAC 3 cut(s) 367, 418, 911
DpnI GATC 5 cut(s) 546, 759, 1041, 1116, 1242
DpnII GATC 5 cut(s) 544, 757, 1039, 1114, 1240
EaeI YGGCCR 3 cut(s) 73, 1386, 1456
Eam1104I CTCTTC 1 cut(s) 612
EarI CTCTTC 1 cut(s) 612
Ecl136II GAGCTC 1 cut(s) 1532
Eco130I CCWWGG 3 cut(s) 375, 687, 693
Eco24I GRGCYC 1 cut(s) 1534
Eco53kI GAGCTC 1 cut(s) 1532
Eco57I CTGAAG 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 254
Eco91I GGTNACC 1 cut(s) 341
EcoICRI GAGCTC 1 cut(s) 1532
EcoNI CCTNNNNNAGG 1 cut(s) 776
EcoO65I GGTNACC 1 cut(s) 341
EcoRII CCWGG 3 cut(s) 955, 1061, 1625
EcoT14I CCWWGG 3 cut(s) 375, 687, 693
EcoT38I GRGCYC 1 cut(s) 1534
ErhI CCWWGG 3 cut(s) 375, 687, 693
FaeI CATG 3 cut(s) 1393, 1457, 1524
FalI AAGNNNNNCTT 2 cut(s) 1004, 1036
FaqI GGGAC 2 cut(s) 963, 1120
FatI CATG 3 cut(s) 1389, 1453, 1520
FauI CCCGC 2 cut(s) 606, 1151
Fnu4HI GCNGC 5 cut(s) 153, 573, 643, 819, 1353
FriOI GRGCYC 1 cut(s) 1534
Fsp4HI GCNGC 5 cut(s) 153, 573, 643, 819, 1353
FspBI CTAG 1 cut(s) 314
GlaI GCGC 2 cut(s) 479, 1564
GluI GCNGC 5 cut(s) 153, 573, 643, 819, 1353
HaeII RGCGCY 1 cut(s) 1566
HaeIII GGCC 8 cut(s) 65, 75, 692, 955, 1388, 1458, 1625, 1639
HapII CCGG 3 cut(s) 712, 1098, 1718
HgaI GACGC 1 cut(s) 469
HhaI GCGC 2 cut(s) 480, 1565
Hin1II CATG 3 cut(s) 1393, 1457, 1524
Hin6I GCGC 2 cut(s) 478, 1563
HinP1I GCGC 2 cut(s) 478, 1563
HincII GTYRAC 2 cut(s) 141, 624
HindII GTYRAC 2 cut(s) 141, 624
HinfI GANTC 7 cut(s) 86, 193, 745, 905, 976, 1028, 1690
HpaII CCGG 3 cut(s) 712, 1098, 1718
HphI GGTGA 6 cut(s) 459, 504, 599, 1386, 1493, 1502
Hpy166II GTNNAC 4 cut(s) 141, 367, 512, 624
Hpy188III TCNNGA 8 cut(s) 46, 328, 542, 761, 1198, 1229, 1508, 1694
Hpy8I GTNNAC 4 cut(s) 141, 367, 512, 624
Hpy99I CGWCG 3 cut(s) 71, 485, 933
HpyCH4III ACNGT 5 cut(s) 341, 664, 799, 1134, 1481
HpyCH4IV ACGT 3 cut(s) 168, 928, 1318
HpyF10VI GCNNNNNNNGC 6 cut(s) 114, 152, 306, 430, 462, 1358
HpySE526I ACGT 3 cut(s) 168, 928, 1318
Hsp92II CATG 3 cut(s) 1393, 1457, 1524
HspAI GCGC 2 cut(s) 478, 1563
Kzo9I GATC 5 cut(s) 544, 757, 1039, 1114, 1240
LmnI GCTCC 3 cut(s) 1529, 1537, 1629
Lsp1109I GCAGC 5 cut(s) 139, 584, 654, 805, 1364
LweI GCATC 3 cut(s) 559, 667, 1103
MaeI CTAG 1 cut(s) 314
MaeII ACGT 3 cut(s) 168, 928, 1318
MaeIII GTNAC 3 cut(s) 341, 1319, 1392
MalI GATC 5 cut(s) 546, 759, 1041, 1116, 1242
MboI GATC 5 cut(s) 544, 757, 1039, 1114, 1240
MfeI CAATTG 3 cut(s) 289, 295, 498
MflI RGATCY 1 cut(s) 1240
MhlI GDGCHC 1 cut(s) 1534
MlsI TGGCCA 2 cut(s) 75, 1388
MluCI AATT 8 cut(s) 289, 295, 498, 812, 854, 1207, 1281, 1308
MluI ACGCGT 1 cut(s) 55
MluNI TGGCCA 2 cut(s) 75, 1388
MlyI GAGTC 3 cut(s) 80, 1037, 1684
MmeI TCCRAC 1 cut(s) 811
Mox20I TGGCCA 2 cut(s) 75, 1388
MscI TGGCCA 2 cut(s) 75, 1388
MseI TTAA 2 cut(s) 882, 1124
MslI CAYNNNNRTG 2 cut(s) 1370, 1458
Msp20I TGGCCA 2 cut(s) 75, 1388
MspI CCGG 3 cut(s) 712, 1098, 1718
MspR9I CCNGG 3 cut(s) 957, 1063, 1627
MunI CAATTG 3 cut(s) 289, 295, 498
Mva1269I GAATGC 1 cut(s) 731
MvaI CCWGG 3 cut(s) 957, 1063, 1627
MvnI CGCG 2 cut(s) 57, 480
MwoI GCNNNNNNNGC 6 cut(s) 114, 152, 306, 430, 462, 1358
NdeII GATC 5 cut(s) 544, 757, 1039, 1114, 1240
NlaIII CATG 3 cut(s) 1393, 1457, 1524
NlaIV GGNNCC 5 cut(s) 710, 1018, 1067, 1631, 1683
NmuCI GTSAC 1 cut(s) 1392
OliI CACNNNNGTG 1 cut(s) 1370
PaeR7I CTCGAG 1 cut(s) 254
PctI GAATGC 1 cut(s) 731
PfeI GAWTC 4 cut(s) 193, 745, 905, 976
PflMI CCANNNNNTGG 1 cut(s) 1075
PkrI GCNGC 5 cut(s) 154, 574, 644, 820, 1354
Ple19I CGATCG 1 cut(s) 547
PleI GAGTC 3 cut(s) 80, 1036, 1684
PpsI GAGTC 3 cut(s) 80, 1036, 1684
Psp124BI GAGCTC 1 cut(s) 1534
Psp6I CCWGG 3 cut(s) 955, 1061, 1625
PspEI GGTNACC 1 cut(s) 341
PspGI CCWGG 3 cut(s) 955, 1061, 1625
PspN4I GGNNCC 5 cut(s) 710, 1018, 1067, 1631, 1683
PspPI GGNCC 2 cut(s) 63, 1623
PspXI VCTCGAGB 1 cut(s) 254
PstI CTGCAG 1 cut(s) 846
PstNI CAGNNNCTG 1 cut(s) 850
PsuI RGATCY 1 cut(s) 1240
PvuI CGATCG 1 cut(s) 547
RsaI GTAC 3 cut(s) 368, 419, 912
RsaNI GTAC 3 cut(s) 367, 418, 911
RseI CAYNNNNRTG 2 cut(s) 1370, 1458
SacI GAGCTC 1 cut(s) 1534
SaqAI TTAA 2 cut(s) 882, 1124
SatI GCNGC 5 cut(s) 153, 573, 643, 819, 1353
Sau3AI GATC 5 cut(s) 544, 757, 1039, 1114, 1240
Sau96I GGNCC 2 cut(s) 63, 1623
SchI GAGTC 3 cut(s) 80, 1037, 1684
ScrFI CCNGG 3 cut(s) 957, 1063, 1627
SduI GDGCHC 1 cut(s) 1534
SfaNI GCATC 3 cut(s) 559, 667, 1103
SfcI CTRYAG 2 cut(s) 798, 842
Sfr274I CTCGAG 1 cut(s) 254
SlaI CTCGAG 1 cut(s) 254
SmiMI CAYNNNNRTG 2 cut(s) 1370, 1458
SmlI CTYRAG 2 cut(s) 254, 1356
SmoI CTYRAG 2 cut(s) 254, 1356
SpeI ACTAGT 1 cut(s) 313
Sse9I AATT 8 cut(s) 289, 295, 498, 812, 854, 1207, 1281, 1308
SsiI CCGC 2 cut(s) 613, 1158
SspI AATATT 1 cut(s) 739
SspMI CTAG 1 cut(s) 314
SstI GAGCTC 1 cut(s) 1534
StyD4I CCNGG 3 cut(s) 955, 1061, 1625
StyI CCWWGG 3 cut(s) 375, 687, 693
TaaI ACNGT 5 cut(s) 341, 664, 799, 1134, 1481
TaiI ACGT 3 cut(s) 171, 931, 1321
TaqI TCGA 7 cut(s) 255, 543, 547, 931, 979, 1113, 1584
TasI AATT 8 cut(s) 289, 295, 498, 812, 854, 1207, 1281, 1308
TatI WGTACW 2 cut(s) 417, 910
TfiI GAWTC 4 cut(s) 193, 745, 905, 976
Tru1I TTAA 2 cut(s) 882, 1124
Tru9I TTAA 2 cut(s) 882, 1124
TscAI CASTG 5 cut(s) 119, 358, 846, 978, 1372
TseFI GTSAC 1 cut(s) 1392
TseI GCWGC 5 cut(s) 152, 572, 642, 818, 1352
Tsp45I GTSAC 1 cut(s) 1392
TspDTI ATGAA 7 cut(s) 575, 974, 994, 1422, 1442, 1586, 1719
TspGWI ACGGA 1 cut(s) 146
TspRI CASTG 5 cut(s) 119, 358, 846, 978, 1372
Van91I CCANNNNNTGG 1 cut(s) 1075
XagI CCTNNNNNAGG 1 cut(s) 776
XapI RAATTY 3 cut(s) 854, 1207, 1308
XhoI CTCGAG 1 cut(s) 254
XspI CTAG 1 cut(s) 314
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.