Rh2AG566900

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000989
Physical Location & Seq
Forward (+)
79821662 .. 79825542
3881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG566900.1

Sequence Viewer

Length: 1863 bp
ATGGCCGGAGTTCGTGTCGTCGGTGGCCGGATCTACGATTCCGAGAACGGAAAAACCTGTCACCAGTGCCGGCAGAAGACTAGGGATTTTGTGGCGTCGTGTAAGAATCTGAAGAAGAACGCTCGCTGCACCATTAGCTTTTGTCATAAATGCCTCTTGAACAGATATGGAGAGAAGGCTGAAGAGGTGGAGCTTTTGGAGGACTGGAATTGCCCCAAATGTAGGGGGAATTGTAATTGTAGTTTCTGCAGGAAGAAACAAGGCCTCAAACCCACTGGTCTACTTGTTCACACAGCCAAGGAAGGTGGGTTTGGTTCGGTTTCGGAGATGTTGACTGCGAGAGGACCGGAGAATTTTGGTATTGAGAGGAAGGTTGCGGAGAAGGTGGCTGTCGATGGAAAGGAGAATTCTGTTGATCAAAAGGTTGAGGCCAGCCTGAATTCAACACAAAATCCTGAGGAGGGACAAGTCAAGAAAACAAAGAGGAAAGGATTGAAGGAAATCCGTAATGGCAGCAGGGATGATCCTAAAGCCTCCGAGGATGGCAGCAGGGATGATCCTAAAGTCTCCGAGGATGGCAGCAGGGATGATCCTAAAGTCCCCAAGGATGGCAGCAGGGATGATCCTAAAGTCTCCAAGGATGTTGCTGTAAAAGAAGTGAAATCAAGAAAGAGAAAAAATAAAGATCGAAGAGAAGAAGCAGATGATGCGAAACTGCAAGGTGAAGAAGTTCAAAATGTTGTGAACAACAATGATGTCAAGAGGAAGAAAAAGGCCAAGGACAAGGTCAAGAGTAGCAAAGAGACTTGCAGAGTTGAGGAATGCACCAAGAATATTCTGAACAAAAAAGTTGAGCCTGAGCTTCCTTTGCCTCAGGGCATTCCCTTAACAAGTGTCTTGGGAATTGAACTGCCTCCTGAGGATGCTGGAAACGCTCTGCAATTTATGGAATTTTGTTCAGCTTTTGGAGAGGTTCTCAAATTCAAAAAAGCACAGGCTGAAGCTATACTTGGTGAATTACTGCGCCAACGAAGTGGGCGAGCTGGCCGCCCTGGACAATACTCCTCACTTATTCGGTTTCACATGCAACTATTATCTCTACTACAGGAGGATATGGGAGAGGAGCCTTCATCTATAGATGAAATGAGCCACAAAAATTCATGGTTCCAAGATCTGGGTAAATGCATCTCTGAATCTGAGTCCGAACACCAATTGAAGGAACTGCCTACAGATTGTTTCAGTAGAGGTGGTGCTGGATATGATATCTTAGACCTCTCTCAGAAGCTCAGACTCTTAACTTACCTTTGTGATGAGGTGATTTGCACTTCTAAACTGAGGAGCTGGATAGAAGAACAGCATGAAAAGATTGTTGAAAGTGAAAAGGAAGCAAAAGGAAAAGTTAATGCAGCCAAAGATAAGGAAAAACTCCTCAAGCGGAAGTTGCAAGATGAGGTGGCGAAGCTTATTATTGCAAAAAATGGTGCTCCTCTGTCAATTTCAGAACATGAAGCTCTTGTTTCACAAATAAAAAGTGAAGCAGCTCAAGCTCATGCTGAGTTGCTTGAAGCAGAGGGCATAGTGCCTAAAAAGAAACATAGACGTGATGCTTTAAGAACAGAGCCTTATCGTGTGGATGTTGATGGCCGCATCTTTTTGAAATTAAAGGGTTACAACAATGGAGAAGATATCGTGCTTCAAGATTTGGGGGCCTGGGATGCAGTTGTATCTAAAGAAGAATGGTTTGTTTATGGTGCGGAGACAAAGGAAGCAATTGACAATTACTGTTCTTCTTTAAGGAGAAAAAAGTCTAGTGGAACCATGTCGCAAACTGTTCCTCGTGAAAGCGATGAAGAAAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

620

Amino Acids

69.5

Weight (kDa)

8.33

Isoelectric Point (pI)

44.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 12 - 111 1.8e-36 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1174
AccI GTMKAC 1 cut(s) 280
AciI CCGC 5 cut(s) 377, 1048, 1435, 1645, 1754
AclWI GGATC 5 cut(s) 38, 518, 551, 584, 617
AcoI YGGCCR 4 cut(s) 3, 25, 1045, 1642
AcsI RAATTY 6 cut(s) 352, 406, 439, 950, 980, 1156
AcuI CTGAAG 3 cut(s) 131, 201, 1020
AcyI GRCGYC 1 cut(s) 95
AfiI CCNNNNNNNGG 5 cut(s) 222, 461, 608, 1174, 1216
AflIII ACRYGT 1 cut(s) 1857
AjiI CACGTC 1 cut(s) 1601
AjnI CCWGG 2 cut(s) 1051, 1709
AjuI GAANNNNNNNTTGG 4 cut(s) 294, 326, 993, 1025
Alw21I GWGCWC 1 cut(s) 1486
Alw26I GTCTC 4 cut(s) 571, 637, 797, 1751
AlwI GGATC 5 cut(s) 38, 518, 551, 584, 617
AoxI GGCC 8 cut(s) 3, 25, 262, 429, 774, 1045, 1642, 1707
ApeKI GCWGC 7 cut(s) 126, 513, 546, 579, 612, 1406, 1538
ApoI RAATTY 6 cut(s) 352, 406, 439, 950, 980, 1156
Asp700I GAANNNNTTC 1 cut(s) 729
AspLEI GCGC 1 cut(s) 1026
AspS9I GGNCC 2 cut(s) 344, 1707
AsuHPI GGTGA 4 cut(s) 53, 734, 1025, 1327
AvaII GGWCC 1 cut(s) 344
AxyI CCTNAGG 3 cut(s) 456, 873, 918
BauI CACGAG 1 cut(s) 1836
BbsI GAAGAC 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 1486
BbvI GCAGC 7 cut(s) 113, 525, 558, 591, 624, 1418, 1550
BccI CCATC 5 cut(s) 389, 536, 569, 602, 1634
BciT130I CCWGG 2 cut(s) 1053, 1711
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 4 cut(s) 571, 637, 797, 1751
BfaI CTAG 2 cut(s) 81, 1809
BfmI CTRYAG 4 cut(s) 247, 1103, 1134, 1227
BglII AGATCT 1 cut(s) 1171
BisI GCNGC 9 cut(s) 127, 514, 547, 580, 613, 1048, 1407, 1539, 1645
BlsI GCNGC 9 cut(s) 128, 515, 548, 581, 614, 1049, 1408, 1540, 1646
Bme1390I CCNGG 2 cut(s) 1053, 1711
Bme18I GGWCC 1 cut(s) 344
BmgBI CACGTC 1 cut(s) 1601
BmgT120I GGNCC 2 cut(s) 344, 1707
BmiI GGNNCC 4 cut(s) 1125, 1166, 1708, 1816
BmrFI CCNGG 2 cut(s) 1053, 1711
BmsI GCATC 6 cut(s) 697, 913, 1194, 1594, 1656, 1705
BpiI GAAGAC 1 cut(s) 83
BplI GAGNNNNNCTC 2 cut(s) 960, 992
Bpu10I CCTNAGC 1 cut(s) 858
BpuEI CTTGAG 2 cut(s) 1415, 1527
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 8 cut(s) 297, 537, 570, 603, 636, 777, 1051, 1710
BsaWI WCCGGW 1 cut(s) 346
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 5 cut(s) 222, 461, 608, 1174, 1216
Bse118I RCCGGY 1 cut(s) 69
Bse1I ACTGG 3 cut(s) 64, 209, 280
Bse21I CCTNAGG 3 cut(s) 456, 873, 918
BseBI CCWGG 2 cut(s) 1053, 1711
BseDI CCNNGG 8 cut(s) 297, 537, 570, 603, 636, 777, 1051, 1710
BseLI CCNNNNNNNGG 5 cut(s) 222, 461, 608, 1174, 1216
BseMII CTCAG 9 cut(s) 447, 849, 887, 909, 1188, 1292, 1300, 1325, 1545
BseNI ACTGG 3 cut(s) 64, 209, 280
BseRI GAGGAG 6 cut(s) 473, 1054, 1136, 1351, 1418, 1476
BseXI GCAGC 7 cut(s) 113, 525, 558, 591, 624, 1418, 1550
BsgI GTGCAG 1 cut(s) 112
BshFI GGCC 8 cut(s) 5, 27, 264, 431, 776, 1047, 1644, 1709
BsiHKAI GWGCWC 1 cut(s) 1486
BsiSI CCGG 4 cut(s) 6, 28, 70, 347
BslFI GGGAC 2 cut(s) 477, 584
BslI CCNNNNNNNGG 5 cut(s) 222, 461, 608, 1174, 1216
BsmAI GTCTC 4 cut(s) 571, 637, 797, 1751
BsmFI GGGAC 2 cut(s) 477, 584
BsmI GAATGC 2 cut(s) 827, 879
BsnI GGCC 8 cut(s) 5, 27, 264, 431, 776, 1047, 1644, 1709
Bsp1286I GDGCHC 1 cut(s) 1486
Bsp143I GATC 8 cut(s) 30, 415, 523, 556, 589, 622, 685, 1171
BspACI CCGC 5 cut(s) 377, 1048, 1435, 1645, 1754
BspANI GGCC 8 cut(s) 5, 27, 264, 431, 776, 1047, 1644, 1709
BspCNI CTCAG 9 cut(s) 448, 850, 886, 910, 1189, 1291, 1299, 1326, 1546
BspLI GGNNCC 4 cut(s) 1125, 1166, 1708, 1816
BspMAI CTGCAG 1 cut(s) 251
BspPI GGATC 5 cut(s) 38, 518, 551, 584, 617
BsrFI RCCGGY 1 cut(s) 69
BsrI ACTGG 3 cut(s) 64, 209, 280
BssAI RCCGGY 1 cut(s) 69
BssECI CCNNGG 8 cut(s) 297, 537, 570, 603, 636, 777, 1051, 1710
BssMI GATC 8 cut(s) 30, 415, 523, 556, 589, 622, 685, 1171
BssNI GRCGYC 1 cut(s) 95
BssSI CACGAG 1 cut(s) 1836
BssT1I CCWWGG 4 cut(s) 297, 603, 636, 777
Bst2BI CACGAG 1 cut(s) 1836
Bst2UI CCWGG 2 cut(s) 1053, 1711
Bst4CI ACNGT 2 cut(s) 1784, 1831
Bst6I CTCTTC 2 cut(s) 177, 685
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 707
BstC8I GCNNGC 5 cut(s) 71, 124, 433, 1041, 1045
BstHHI GCGC 1 cut(s) 1026
BstKTI GATC 8 cut(s) 33, 418, 526, 559, 592, 625, 688, 1174
BstMAI GTCTC 4 cut(s) 571, 637, 797, 1751
BstMBI GATC 8 cut(s) 30, 415, 523, 556, 589, 622, 685, 1171
BstMWI GCNNNNNNNGC 7 cut(s) 135, 707, 868, 932, 1441, 1544, 1715
BstNI CCWGG 2 cut(s) 1053, 1711
BstNSI RCATGY 2 cut(s) 1087, 1861
BstSCI CCNGG 2 cut(s) 1051, 1709
BstSFI CTRYAG 4 cut(s) 247, 1103, 1134, 1227
BstV1I GCAGC 7 cut(s) 113, 525, 558, 591, 624, 1418, 1550
BstV2I GAAGAC 1 cut(s) 83
BstX2I RGATCY 2 cut(s) 30, 1171
BstXI CCANNNNNNTGG 1 cut(s) 1034
BstYI RGATCY 2 cut(s) 30, 1171
Bsu36I CCTNAGG 3 cut(s) 456, 873, 918
BsuRI GGCC 8 cut(s) 5, 27, 264, 431, 776, 1047, 1644, 1709
BtrI CACGTC 1 cut(s) 1601
BtsIMutI CAGTG 2 cut(s) 71, 273
Cac8I GCNNGC 5 cut(s) 71, 124, 433, 1041, 1045
CfoI GCGC 1 cut(s) 1026
Cfr10I RCCGGY 1 cut(s) 69
Cfr13I GGNCC 2 cut(s) 344, 1707
CseI GACGC 1 cut(s) 84
CspCI CAANNNNNGTGG 2 cut(s) 286, 321
CviAII CATG 7 cut(s) 1084, 1161, 1358, 1505, 1550, 1819, 1858
DpnI GATC 8 cut(s) 32, 417, 525, 558, 591, 624, 687, 1173
DpnII GATC 8 cut(s) 30, 415, 523, 556, 589, 622, 685, 1171
EaeI YGGCCR 4 cut(s) 3, 25, 1045, 1642
Eam1104I CTCTTC 2 cut(s) 177, 685
EarI CTCTTC 2 cut(s) 177, 685
Eco130I CCWWGG 4 cut(s) 297, 603, 636, 777
Eco147I AGGCCT 1 cut(s) 264
Eco32I GATATC 2 cut(s) 1264, 1687
Eco47I GGWCC 1 cut(s) 344
Eco57I CTGAAG 3 cut(s) 131, 201, 1020
Eco81I CCTNAGG 3 cut(s) 456, 873, 918
EcoO109I RGGNCCY 1 cut(s) 1707
EcoRI GAATTC 2 cut(s) 406, 439
EcoRII CCWGG 2 cut(s) 1051, 1709
EcoRV GATATC 2 cut(s) 1264, 1687
EcoT14I CCWWGG 4 cut(s) 297, 603, 636, 777
EcoT22I ATGCAT 1 cut(s) 1187
ErhI CCWWGG 4 cut(s) 297, 603, 636, 777
FaeI CATG 7 cut(s) 1087, 1164, 1361, 1508, 1553, 1822, 1861
FalI AAGNNNNNCTT 2 cut(s) 993, 1025
FaqI GGGAC 2 cut(s) 477, 584
FatI CATG 7 cut(s) 1083, 1160, 1357, 1504, 1549, 1818, 1857
FbaI TGATCA 1 cut(s) 415
FblI GTMKAC 1 cut(s) 280
Fnu4HI GCNGC 9 cut(s) 127, 514, 547, 580, 613, 1048, 1407, 1539, 1645
Fsp4HI GCNGC 9 cut(s) 127, 514, 547, 580, 613, 1048, 1407, 1539, 1645
FspBI CTAG 2 cut(s) 81, 1809
GlaI GCGC 1 cut(s) 1025
GluI GCNGC 9 cut(s) 127, 514, 547, 580, 613, 1048, 1407, 1539, 1645
HaeIII GGCC 8 cut(s) 5, 27, 264, 431, 776, 1047, 1644, 1709
HapII CCGG 4 cut(s) 6, 28, 70, 347
HgaI GACGC 1 cut(s) 84
HhaI GCGC 1 cut(s) 1026
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 7 cut(s) 1087, 1164, 1361, 1508, 1553, 1822, 1861
Hin6I GCGC 1 cut(s) 1024
HinP1I GCGC 1 cut(s) 1024
HincII GTYRAC 1 cut(s) 333
HindII GTYRAC 1 cut(s) 333
HindIII AAGCTT 1 cut(s) 1460
HinfI GANTC 5 cut(s) 38, 106, 1193, 1199, 1290
HpaII CCGG 4 cut(s) 6, 28, 70, 347
HphI GGTGA 4 cut(s) 53, 734, 1025, 1327
Hpy166II GTNNAC 4 cut(s) 281, 289, 333, 745
Hpy188III TCNNGA 9 cut(s) 157, 455, 472, 666, 760, 790, 917, 1697, 1838
Hpy8I GTNNAC 4 cut(s) 281, 289, 333, 745
Hpy99I CGWCG 2 cut(s) 23, 100
HpyAV CCTTC 7 cut(s) 169, 296, 364, 376, 490, 1137, 1210
HpyCH4III ACNGT 2 cut(s) 1784, 1831
HpyCH4IV ACGT 1 cut(s) 1600
HpyF10VI GCNNNNNNNGC 7 cut(s) 135, 707, 868, 932, 1441, 1544, 1715
HpySE526I ACGT 1 cut(s) 1600
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 7 cut(s) 1087, 1164, 1361, 1508, 1553, 1822, 1861
HspAI GCGC 1 cut(s) 1024
KroI GCCGGC 1 cut(s) 69
KroNI GCCGGC 1 cut(s) 71
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 8 cut(s) 30, 415, 523, 556, 589, 622, 685, 1171
LmnI GCTCC 4 cut(s) 190, 1123, 1338, 1489
Lsp1109I GCAGC 7 cut(s) 113, 525, 558, 591, 624, 1418, 1550
LweI GCATC 6 cut(s) 697, 913, 1194, 1594, 1656, 1705
MaeI CTAG 2 cut(s) 81, 1809
MaeII ACGT 1 cut(s) 1600
MaeIII GTNAC 2 cut(s) 59, 1667
MalI GATC 8 cut(s) 32, 417, 525, 558, 591, 624, 687, 1173
MboI GATC 8 cut(s) 30, 415, 523, 556, 589, 622, 685, 1171
MfeI CAATTG 2 cut(s) 1211, 1770
MflI RGATCY 2 cut(s) 30, 1171
MhlI GDGCHC 1 cut(s) 1486
MlyI GAGTC 2 cut(s) 1208, 1284
Mph1103I ATGCAT 1 cut(s) 1187
MroNI GCCGGC 1 cut(s) 69
MroXI GAANNNNTTC 1 cut(s) 729
MseI TTAA 6 cut(s) 887, 1295, 1401, 1610, 1661, 1793
MslI CAYNNNNRTG 1 cut(s) 1599
MspI CCGG 4 cut(s) 6, 28, 70, 347
MspR9I CCNGG 2 cut(s) 1053, 1711
MunI CAATTG 2 cut(s) 1211, 1770
Mva1269I GAATGC 2 cut(s) 827, 879
MvaI CCWGG 2 cut(s) 1053, 1711
MwoI GCNNNNNNNGC 7 cut(s) 135, 707, 868, 932, 1441, 1544, 1715
NaeI GCCGGC 1 cut(s) 71
NdeII GATC 8 cut(s) 30, 415, 523, 556, 589, 622, 685, 1171
NgoMIV GCCGGC 1 cut(s) 69
NlaIII CATG 7 cut(s) 1087, 1164, 1361, 1508, 1553, 1822, 1861
NlaIV GGNNCC 4 cut(s) 1125, 1166, 1708, 1816
NmuCI GTSAC 1 cut(s) 59
NsiI ATGCAT 1 cut(s) 1187
NspI RCATGY 2 cut(s) 1087, 1861
PceI AGGCCT 1 cut(s) 264
PciI ACATGT 1 cut(s) 1857
PcsI WCGNNNNNNNCGW 1 cut(s) 1036
PctI GAATGC 2 cut(s) 827, 879
PdiI GCCGGC 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 729
PfeI GAWTC 3 cut(s) 38, 106, 1193
PflFI GACNNNGTC 1 cut(s) 785
PflMI CCANNNNNTGG 1 cut(s) 1174
PkrI GCNGC 9 cut(s) 128, 515, 548, 581, 614, 1049, 1408, 1540, 1646
PleI GAGTC 2 cut(s) 1207, 1284
PpsI GAGTC 2 cut(s) 1207, 1284
PscI ACATGT 1 cut(s) 1857
Psp6I CCWGG 2 cut(s) 1051, 1709
PspGI CCWGG 2 cut(s) 1051, 1709
PspN4I GGNNCC 4 cut(s) 1125, 1166, 1708, 1816
PspPI GGNCC 2 cut(s) 344, 1707
PstI CTGCAG 1 cut(s) 251
PsuI RGATCY 2 cut(s) 30, 1171
PsyI GACNNNGTC 1 cut(s) 785
RseI CAYNNNNRTG 1 cut(s) 1599
SaqAI TTAA 6 cut(s) 887, 1295, 1401, 1610, 1661, 1793
SatI GCNGC 9 cut(s) 127, 514, 547, 580, 613, 1048, 1407, 1539, 1645
Sau3AI GATC 8 cut(s) 30, 415, 523, 556, 589, 622, 685, 1171
Sau96I GGNCC 2 cut(s) 344, 1707
SchI GAGTC 2 cut(s) 1208, 1284
ScrFI CCNGG 2 cut(s) 1053, 1711
SduI GDGCHC 1 cut(s) 1486
SfaNI GCATC 6 cut(s) 697, 913, 1194, 1594, 1656, 1705
SfcI CTRYAG 4 cut(s) 247, 1103, 1134, 1227
SinI GGWCC 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 1599
SmlI CTYRAG 2 cut(s) 1430, 1542
SmoI CTYRAG 2 cut(s) 1430, 1542
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 5 cut(s) 377, 1048, 1435, 1645, 1754
SspI AATATT 1 cut(s) 835
SspMI CTAG 2 cut(s) 81, 1809
StuI AGGCCT 1 cut(s) 264
StyD4I CCNGG 2 cut(s) 1051, 1709
StyI CCWWGG 4 cut(s) 297, 603, 636, 777
TaaI ACNGT 2 cut(s) 1784, 1831
TaiI ACGT 1 cut(s) 1603
TaqI TCGA 2 cut(s) 393, 688
TauI GCSGC 2 cut(s) 1050, 1647
TfiI GAWTC 3 cut(s) 38, 106, 1193
Tru1I TTAA 6 cut(s) 887, 1295, 1401, 1610, 1661, 1793
Tru9I TTAA 6 cut(s) 887, 1295, 1401, 1610, 1661, 1793
TscAI CASTG 2 cut(s) 71, 280
TseFI GTSAC 1 cut(s) 59
TseI GCWGC 7 cut(s) 126, 513, 546, 579, 612, 1406, 1538
Tsp45I GTSAC 1 cut(s) 59
TspDTI ATGAA 6 cut(s) 1119, 1149, 1155, 1374, 1521, 1863
TspGWI ACGGA 2 cut(s) 63, 494
TspRI CASTG 2 cut(s) 71, 280
Tth111I GACNNNGTC 1 cut(s) 785
Van91I CCANNNNNTGG 1 cut(s) 1174
VpaK11BI GGWCC 1 cut(s) 344
XapI RAATTY 6 cut(s) 352, 406, 439, 950, 980, 1156
XceI RCATGY 2 cut(s) 1087, 1861
XmiI GTMKAC 1 cut(s) 280
XmnI GAANNNNTTC 1 cut(s) 729
XspI CTAG 2 cut(s) 81, 1809
Zsp2I ATGCAT 1 cut(s) 1187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.