Rh2CG549100

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000989
Physical Location & Seq
Forward (+)
72936788 .. 72940808
4021 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG549100.1

Sequence Viewer

Length: 1734 bp
ATGGTTGTGATATTGGGTGTGATGATTGGATATAGATATGGAGAGAAGGCTGAAGAGGTGGAGCTTTTGGAGGACTGGAACTGCCCCAAATGTAGGGGGAATTGTAATTGTAGTTTCTGCAGGAAGAAACAAGGCCTCAAACCCACTGGTCTACTTGTTCACACAGCCAAGGAAGGTGGGTTCGGTTCGGTTTCGGAGATGCTGACTGCCAGAGGACCGGAGAATTTTGGTATTGAGAGGAAGGTTGCGGAGAAGGTGGCTGTCGATGGAAAGGAGAACTCTGTTGATCAAAAGATTGAGGCCAGCCTGAATTCAACACAAAATCCTGAGGAGGGACAAGTCAAGAAAACTAAGAGGAAAGGATTGAAGGAAATCCGTAATGGCAGCAGGGATGATCCTAAAGTCTCCGAGGATGGCAGCAGGGATGATCCTAAAGTCTCCGAGAATGGCAGCAGGGATGATCCTAAAGTCCCCGAGGATAGCAGCAGGGATGATCCTAAAGTCTCCAAGGATGTTGCTGTAAAAGAAGTGAAATCAAGAAAGAGAAAAAATAAAGATCGAAGAGAAGAAGCAGATGATGCGAAACTGCAAGGTGAAGAAGTTCAAACTGTTGTGAACAACAATGATGTCAAGAGGAAGAAAAAGGCCAAGGACAAGGTCAAGAGTAGCAAAGAGACTTGCAGAGTTGAGGAATGCACCAAGAATATTCTGAACAAAAAAGTTGAGCCTGAGCTTCCTTTGCCTGAGGGCATTCCCTTAACAAGTGTCTTGGGAATTGAACTGCCTCCTGAGGATGCTGGAAATGCTCTGCAATTTATGGAATTTTGTTCAGCTTTTGGAGAGGTTCTCAAATTCAAAAAAGCACAGGCTGAATCTATAATTGGTGAATTACTGCGCCAACGAAGTGGGCGAGCTGGCCGCCCTGGACAATACTCCTCACTTATTCGGTTTCACATGCAACTATTATCTCTACTACAGGAGGATATGGGAGAGGAGCCTTCATCTATAGATGAAATGAGCCACAAAAATTCATGGTTCCAAGATCTGGGTAAATGCATCTCTGAATCTGAGTCTGAACACCTATTGAAGGAACTGCCTACAGATTGTTTCAGTAGAGGTGGTGCTGGATATGATATCTTAGACCTCTCTCAGAAGCTCAGACTCTTAACTTACCTTTGTGATGAGGTGATTTGCACTTCTAAACTGAGGAGCTGGATAGAAGAACAGCATGAAAAGATTGTTGAAAGCGAAAAGGAAGCAAAAGGAAAAGTTAATGCAGCCAAAGATAAGGAAAAACTCCTCAAGCGGAAGTTGCAAGATGAGGTGGCGAAGCTTATTATTGCAAAAAATGGTGCTCCTCTGTCAATTTCAGAACATGAAGCTCTTGTTTCACAAATAAAAAGTGAAGCAGCTCAAGCTCATGCTGAGTTGCTTGAAGCAGAGGGCATAGTGCCTAAAAAGAAACATAGACGTGATGCTTTAAGAACGGAGCCTTATCGTGTGGATGTTGATGGCCGCATCTTTTTGAAATTAAAGGGTTACAACAATGGAGAAGATATCGTGCTTCAAGATTTGGGGGCCTGGGATGCAGTTGTATCTAAAGAAGAATGGTTTGTTTATGGTGCGGAGACAAAGGAAGCAATTGAAAATTACTGTTCTTCTTTAAGGAGAAAAAAGTCTAGTGGAACCATGTCGCAAACTGTTCCTCGTGAAAGTGATGAAGAAAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

577

Amino Acids

64.68

Weight (kDa)

6.24

Isoelectric Point (pI)

44.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 10 - 68 2.8e-21 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1045
AccI GTMKAC 1 cut(s) 151
AciI CCGC 5 cut(s) 248, 919, 1306, 1516, 1625
AclWI GGATC 4 cut(s) 389, 422, 455, 488
AcoI YGGCCR 2 cut(s) 916, 1513
AcsI RAATTY 5 cut(s) 223, 310, 821, 851, 1027
AcuI CTGAAG 1 cut(s) 72
AfiI CCNNNNNNNGG 4 cut(s) 93, 332, 1045, 1087
AflIII ACRYGT 1 cut(s) 1728
AjiI CACGTC 1 cut(s) 1472
AjnI CCWGG 2 cut(s) 922, 1580
AjuI GAANNNNNNNTTGG 2 cut(s) 864, 896
AloI GAACNNNNNNTCC 2 cut(s) 164, 196
Alw21I GWGCWC 1 cut(s) 1357
Alw26I GTCTC 5 cut(s) 409, 442, 508, 668, 1622
AlwI GGATC 4 cut(s) 389, 422, 455, 488
Ama87I CYCGRG 1 cut(s) 473
AoxI GGCC 6 cut(s) 133, 300, 645, 916, 1513, 1578
ApeKI GCWGC 6 cut(s) 384, 417, 450, 483, 1277, 1409
ApoI RAATTY 5 cut(s) 223, 310, 821, 851, 1027
Asp700I GAANNNNTTC 1 cut(s) 600
AspLEI GCGC 1 cut(s) 897
AspS9I GGNCC 2 cut(s) 215, 1578
AsuHPI GGTGA 3 cut(s) 605, 896, 1198
AvaI CYCGRG 1 cut(s) 473
AvaII GGWCC 1 cut(s) 215
AxyI CCTNAGG 3 cut(s) 327, 744, 789
BauI CACGAG 1 cut(s) 1707
Bbv12I GWGCWC 1 cut(s) 1357
BbvI GCAGC 6 cut(s) 396, 429, 462, 495, 1289, 1421
BccI CCATC 3 cut(s) 260, 407, 1505
BciT130I CCWGG 2 cut(s) 924, 1582
BclI TGATCA 1 cut(s) 286
BcoDI GTCTC 5 cut(s) 409, 442, 508, 668, 1622
BfaI CTAG 1 cut(s) 1680
BfmI CTRYAG 4 cut(s) 118, 974, 1005, 1098
BglII AGATCT 1 cut(s) 1042
BisI GCNGC 8 cut(s) 385, 418, 451, 484, 919, 1278, 1410, 1516
BlsI GCNGC 8 cut(s) 386, 419, 452, 485, 920, 1279, 1411, 1517
Bme1390I CCNGG 2 cut(s) 924, 1582
Bme18I GGWCC 1 cut(s) 215
BmeT110I CYCGRG 1 cut(s) 473
BmgBI CACGTC 1 cut(s) 1472
BmgT120I GGNCC 2 cut(s) 215, 1578
BmiI GGNNCC 5 cut(s) 996, 1037, 1491, 1579, 1687
BmrFI CCNGG 2 cut(s) 924, 1582
BmsI GCATC 7 cut(s) 189, 568, 784, 1065, 1465, 1527, 1576
BplI GAGNNNNNCTC 2 cut(s) 831, 863
Bpu10I CCTNAGC 1 cut(s) 729
BpuEI CTTGAG 2 cut(s) 1286, 1398
BsaJI CCNNGG 7 cut(s) 168, 408, 474, 507, 648, 922, 1581
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 4 cut(s) 93, 332, 1045, 1087
Bse1I ACTGG 2 cut(s) 80, 151
Bse21I CCTNAGG 3 cut(s) 327, 744, 789
BseBI CCWGG 2 cut(s) 924, 1582
BseDI CCNNGG 7 cut(s) 168, 408, 474, 507, 648, 922, 1581
BseGI GGATG 9 cut(s) 397, 418, 430, 463, 496, 517, 799, 1510, 1591
BseLI CCNNNNNNNGG 4 cut(s) 93, 332, 1045, 1087
BseMII CTCAG 9 cut(s) 318, 720, 735, 780, 1059, 1163, 1171, 1196, 1416
BseNI ACTGG 2 cut(s) 80, 151
BseRI GAGGAG 6 cut(s) 344, 925, 1007, 1222, 1289, 1347
BseXI GCAGC 6 cut(s) 396, 429, 462, 495, 1289, 1421
BshFI GGCC 6 cut(s) 135, 302, 647, 918, 1515, 1580
BsiHKAI GWGCWC 1 cut(s) 1357
BsiHKCI CYCGRG 1 cut(s) 473
BsiSI CCGG 1 cut(s) 218
BslFI GGGAC 2 cut(s) 348, 455
BslI CCNNNNNNNGG 4 cut(s) 93, 332, 1045, 1087
BsmAI GTCTC 5 cut(s) 409, 442, 508, 668, 1622
BsmFI GGGAC 2 cut(s) 348, 455
BsmI GAATGC 2 cut(s) 698, 750
BsnI GGCC 6 cut(s) 135, 302, 647, 918, 1515, 1580
BsoBI CYCGRG 1 cut(s) 473
Bsp1286I GDGCHC 1 cut(s) 1357
Bsp143I GATC 7 cut(s) 286, 394, 427, 460, 493, 556, 1042
BspACI CCGC 5 cut(s) 248, 919, 1306, 1516, 1625
BspANI GGCC 6 cut(s) 135, 302, 647, 918, 1515, 1580
BspCNI CTCAG 9 cut(s) 319, 721, 736, 781, 1060, 1162, 1170, 1197, 1417
BspLI GGNNCC 5 cut(s) 996, 1037, 1491, 1579, 1687
BspMAI CTGCAG 1 cut(s) 122
BspPI GGATC 4 cut(s) 389, 422, 455, 488
BsrI ACTGG 2 cut(s) 80, 151
BssECI CCNNGG 7 cut(s) 168, 408, 474, 507, 648, 922, 1581
BssMI GATC 7 cut(s) 286, 394, 427, 460, 493, 556, 1042
BssSI CACGAG 1 cut(s) 1707
BssT1I CCWWGG 3 cut(s) 168, 507, 648
Bst2BI CACGAG 1 cut(s) 1707
Bst2UI CCWGG 2 cut(s) 924, 1582
Bst4CI ACNGT 3 cut(s) 610, 1655, 1702
Bst6I CTCTTC 2 cut(s) 48, 556
BstAPI GCANNNNNTGC 1 cut(s) 578
BstC8I GCNNGC 3 cut(s) 304, 912, 916
BstENI CCTNNNNNAGG 1 cut(s) 1085
BstF5I GGATG 9 cut(s) 397, 418, 430, 463, 496, 517, 799, 1510, 1591
BstHHI GCGC 1 cut(s) 897
BstKTI GATC 7 cut(s) 289, 397, 430, 463, 496, 559, 1045
BstMAI GTCTC 5 cut(s) 409, 442, 508, 668, 1622
BstMBI GATC 7 cut(s) 286, 394, 427, 460, 493, 556, 1042
BstMWI GCNNNNNNNGC 6 cut(s) 578, 739, 803, 1312, 1415, 1586
BstNI CCWGG 2 cut(s) 924, 1582
BstNSI RCATGY 2 cut(s) 958, 1732
BstSCI CCNGG 2 cut(s) 922, 1580
BstSFI CTRYAG 4 cut(s) 118, 974, 1005, 1098
BstV1I GCAGC 6 cut(s) 396, 429, 462, 495, 1289, 1421
BstX2I RGATCY 1 cut(s) 1042
BstXI CCANNNNNNTGG 1 cut(s) 905
BstYI RGATCY 1 cut(s) 1042
Bsu36I CCTNAGG 3 cut(s) 327, 744, 789
BsuRI GGCC 6 cut(s) 135, 302, 647, 918, 1515, 1580
BtrI CACGTC 1 cut(s) 1472
BtsCI GGATG 9 cut(s) 397, 418, 430, 463, 496, 517, 799, 1510, 1591
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 3 cut(s) 304, 912, 916
CfoI GCGC 1 cut(s) 897
Cfr13I GGNCC 2 cut(s) 215, 1578
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
CviAII CATG 7 cut(s) 955, 1032, 1229, 1376, 1421, 1690, 1729
DpnI GATC 7 cut(s) 288, 396, 429, 462, 495, 558, 1044
DpnII GATC 7 cut(s) 286, 394, 427, 460, 493, 556, 1042
EaeI YGGCCR 2 cut(s) 916, 1513
Eam1104I CTCTTC 2 cut(s) 48, 556
EarI CTCTTC 2 cut(s) 48, 556
Eco130I CCWWGG 3 cut(s) 168, 507, 648
Eco147I AGGCCT 1 cut(s) 135
Eco32I GATATC 2 cut(s) 1135, 1558
Eco47I GGWCC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 72
Eco81I CCTNAGG 3 cut(s) 327, 744, 789
Eco88I CYCGRG 1 cut(s) 473
EcoNI CCTNNNNNAGG 1 cut(s) 1085
EcoO109I RGGNCCY 1 cut(s) 1578
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 2 cut(s) 922, 1580
EcoRV GATATC 2 cut(s) 1135, 1558
EcoT14I CCWWGG 3 cut(s) 168, 507, 648
EcoT22I ATGCAT 1 cut(s) 1058
ErhI CCWWGG 3 cut(s) 168, 507, 648
FaeI CATG 7 cut(s) 958, 1035, 1232, 1379, 1424, 1693, 1732
FaqI GGGAC 2 cut(s) 348, 455
FatI CATG 7 cut(s) 954, 1031, 1228, 1375, 1420, 1689, 1728
FbaI TGATCA 1 cut(s) 286
FblI GTMKAC 1 cut(s) 151
Fnu4HI GCNGC 8 cut(s) 385, 418, 451, 484, 919, 1278, 1410, 1516
FokI GGATG 9 cut(s) 404, 425, 437, 470, 503, 524, 806, 1517, 1598
Fsp4HI GCNGC 8 cut(s) 385, 418, 451, 484, 919, 1278, 1410, 1516
FspBI CTAG 1 cut(s) 1680
GlaI GCGC 1 cut(s) 896
GluI GCNGC 8 cut(s) 385, 418, 451, 484, 919, 1278, 1410, 1516
HaeIII GGCC 6 cut(s) 135, 302, 647, 918, 1515, 1580
HapII CCGG 1 cut(s) 218
HhaI GCGC 1 cut(s) 897
Hin1II CATG 7 cut(s) 958, 1035, 1232, 1379, 1424, 1693, 1732
Hin6I GCGC 1 cut(s) 895
HinP1I GCGC 1 cut(s) 895
HindIII AAGCTT 1 cut(s) 1331
HinfI GANTC 4 cut(s) 872, 1064, 1070, 1161
HpaII CCGG 1 cut(s) 218
HphI GGTGA 3 cut(s) 605, 896, 1198
Hpy166II GTNNAC 3 cut(s) 152, 160, 616
Hpy188III TCNNGA 8 cut(s) 326, 343, 537, 631, 661, 788, 1568, 1709
Hpy8I GTNNAC 3 cut(s) 152, 160, 616
HpyAV CCTTC 7 cut(s) 40, 167, 235, 247, 361, 1008, 1081
HpyCH4III ACNGT 3 cut(s) 610, 1655, 1702
HpyCH4IV ACGT 1 cut(s) 1471
HpyF10VI GCNNNNNNNGC 6 cut(s) 578, 739, 803, 1312, 1415, 1586
HpySE526I ACGT 1 cut(s) 1471
Hsp92II CATG 7 cut(s) 958, 1035, 1232, 1379, 1424, 1693, 1732
HspAI GCGC 1 cut(s) 895
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 7 cut(s) 286, 394, 427, 460, 493, 556, 1042
LmnI GCTCC 5 cut(s) 61, 994, 1209, 1360, 1489
Lsp1109I GCAGC 6 cut(s) 396, 429, 462, 495, 1289, 1421
LweI GCATC 7 cut(s) 189, 568, 784, 1065, 1465, 1527, 1576
MaeI CTAG 1 cut(s) 1680
MaeII ACGT 1 cut(s) 1471
MaeIII GTNAC 1 cut(s) 1538
MalI GATC 7 cut(s) 288, 396, 429, 462, 495, 558, 1044
MboI GATC 7 cut(s) 286, 394, 427, 460, 493, 556, 1042
MfeI CAATTG 1 cut(s) 1641
MflI RGATCY 1 cut(s) 1042
MhlI GDGCHC 1 cut(s) 1357
MlyI GAGTC 2 cut(s) 1079, 1155
Mph1103I ATGCAT 1 cut(s) 1058
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 6 cut(s) 758, 1166, 1272, 1481, 1532, 1664
MslI CAYNNNNRTG 1 cut(s) 1470
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 2 cut(s) 924, 1582
MunI CAATTG 1 cut(s) 1641
Mva1269I GAATGC 2 cut(s) 698, 750
MvaI CCWGG 2 cut(s) 924, 1582
MwoI GCNNNNNNNGC 6 cut(s) 578, 739, 803, 1312, 1415, 1586
NdeII GATC 7 cut(s) 286, 394, 427, 460, 493, 556, 1042
NlaIII CATG 7 cut(s) 958, 1035, 1232, 1379, 1424, 1693, 1732
NlaIV GGNNCC 5 cut(s) 996, 1037, 1491, 1579, 1687
NsiI ATGCAT 1 cut(s) 1058
NspI RCATGY 2 cut(s) 958, 1732
PceI AGGCCT 1 cut(s) 135
PciI ACATGT 1 cut(s) 1728
PcsI WCGNNNNNNNCGW 1 cut(s) 907
PctI GAATGC 2 cut(s) 698, 750
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 2 cut(s) 872, 1064
PflFI GACNNNGTC 1 cut(s) 656
PflMI CCANNNNNTGG 1 cut(s) 1045
PkrI GCNGC 8 cut(s) 386, 419, 452, 485, 920, 1279, 1411, 1517
PleI GAGTC 2 cut(s) 1078, 1155
PpsI GAGTC 2 cut(s) 1078, 1155
PscI ACATGT 1 cut(s) 1728
Psp6I CCWGG 2 cut(s) 922, 1580
PspGI CCWGG 2 cut(s) 922, 1580
PspN4I GGNNCC 5 cut(s) 996, 1037, 1491, 1579, 1687
PspPI GGNCC 2 cut(s) 215, 1578
PstI CTGCAG 1 cut(s) 122
PsuI RGATCY 1 cut(s) 1042
PsyI GACNNNGTC 1 cut(s) 656
RseI CAYNNNNRTG 1 cut(s) 1470
SaqAI TTAA 6 cut(s) 758, 1166, 1272, 1481, 1532, 1664
SatI GCNGC 8 cut(s) 385, 418, 451, 484, 919, 1278, 1410, 1516
Sau3AI GATC 7 cut(s) 286, 394, 427, 460, 493, 556, 1042
Sau96I GGNCC 2 cut(s) 215, 1578
SchI GAGTC 2 cut(s) 1079, 1155
ScrFI CCNGG 2 cut(s) 924, 1582
SduI GDGCHC 1 cut(s) 1357
SfaNI GCATC 7 cut(s) 189, 568, 784, 1065, 1465, 1527, 1576
SfcI CTRYAG 4 cut(s) 118, 974, 1005, 1098
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 1470
SmlI CTYRAG 2 cut(s) 1301, 1413
SmoI CTYRAG 2 cut(s) 1301, 1413
SseBI AGGCCT 1 cut(s) 135
SsiI CCGC 5 cut(s) 248, 919, 1306, 1516, 1625
SspI AATATT 1 cut(s) 706
SspMI CTAG 1 cut(s) 1680
StuI AGGCCT 1 cut(s) 135
StyD4I CCNGG 2 cut(s) 922, 1580
StyI CCWWGG 3 cut(s) 168, 507, 648
TaaI ACNGT 3 cut(s) 610, 1655, 1702
TaiI ACGT 1 cut(s) 1474
TaqI TCGA 2 cut(s) 264, 559
TauI GCSGC 2 cut(s) 921, 1518
TfiI GAWTC 2 cut(s) 872, 1064
Tru1I TTAA 6 cut(s) 758, 1166, 1272, 1481, 1532, 1664
Tru9I TTAA 6 cut(s) 758, 1166, 1272, 1481, 1532, 1664
TscAI CASTG 1 cut(s) 151
TseI GCWGC 6 cut(s) 384, 417, 450, 483, 1277, 1409
TspDTI ATGAA 6 cut(s) 990, 1020, 1026, 1245, 1392, 1734
TspGWI ACGGA 2 cut(s) 365, 1502
TspRI CASTG 1 cut(s) 151
Tth111I GACNNNGTC 1 cut(s) 656
Van91I CCANNNNNTGG 1 cut(s) 1045
VpaK11BI GGWCC 1 cut(s) 215
XagI CCTNNNNNAGG 1 cut(s) 1085
XapI RAATTY 5 cut(s) 223, 310, 821, 851, 1027
XceI RCATGY 2 cut(s) 958, 1732
XmiI GTMKAC 1 cut(s) 151
XmnI GAANNNNTTC 1 cut(s) 600
XspI CTAG 1 cut(s) 1680
Zsp2I ATGCAT 1 cut(s) 1058
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.