Rh2DG589500

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000989
Physical Location & Seq
Forward (+)
82253132 .. 82257134
4003 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG589500.1

Sequence Viewer

Length: 1701 bp
ATGGATGTGATATTGGGTGTGATGATTGGATATAGATATGGAGAGAAGGCTGAAGAGGTGGAGCTTTTGGAGGACTGGAATTGCCCCAAATGTAGGGGAAATTGTAATTGTAGTTTCTGCAGGAAGAAACAAGGCCTCAAACCCACTGGCCTACTTGTTCACACAGCCAAGGAAGGTGGGTTCGGTTCGGTTTCGGAGATGTTGACTGCGAGAGGACCGGAGAATTTTGGTATTGAGAGGAAGGTTGCGGAGAAGGTGGCTGTCGATGGAAAGGAGAATTCTGTTGATCAAAAGGTTGAGGCCAGCCTGAATTCAACACAAAATCCTGAGGAGGGACAAGTCAAGAAAACAAAGAGGAAAGGATTGAAGGAAATCCGTAATGGCAGCAGGGATGATCCTAAAGCCTCCGAGGATGGCAGCAGGGATGATCCTAAAGTCCCCAAGGATGGCAGCAGGGATGATCCTAAAGTCTCCAAGGATGTTGCTGTAAAAGAAGTGAAATCAAGAAAGAGAAAAAATAAAGATCGAAGAGAAGAAGCAGATGATGCGAAACTGCAAGGTGAAGAAGTTCAAAATGTTGTGAACAACAATGATGTTAAGAGGAAGAAAAAGGCCAAGGACAAGGTCAAGAGTAGCAAAGAGACTTGCAGAGTTGAGGAATGCACCAAGAATATTCTGAACAAAAAAGTTGAGCCTGAGCTTCCTTTGCCTGAGGGCATTCCCTTAACAAGTGTCTTGGGAATTGAACTGCCTCCTGAGGATGCTGGAAACGCTCTGCAATTTATGGAATTTTGTTCAGCTTTTGGAGAGGTTCTCAAATTCAAAAAAGCACAGGCTGAATCTATACTTGGTGAATTACTGCGCCAACGAAGTGGGCGAGCTGGCCGCCCTGGACAATACTCCTCTCTTATTCGGTTTCACATGCAACTATTATCTCTACTACAGGAGGATATGGGAGAGGAGCCTTCATCTATAGATGGAATGAGCCACAAAAATTCATGGTTCCAAGATCTGGGTAAATGCATCTCTGAATCTGAGTCCGAACACCAAGTGAAGGAACTGCCTACAGATTGTTTCAGTAGAGGTGGTGCTGGATATGATATCTTAGACCTCTCTCAGAAGCTCAGACTCTTAACTTACCTTTGTGATGAGGTGATTTGCACTTCTAAACTGAGGAGCTGGATAGAAGAACAGCATGAAAAGATTGTTGAAAGCGAAAAGGAAGCAAAAGGAAAAGTTAATGCAGCCAAAGATAAGGAAAAACTCCTCAAGCGGAAGTTGCAAGATGAGGTGGCGAAGCTTATTATTGCAAAAAATGGTGCTCCTCTGTCAATTTCAGAACATGAAGCTCTTGTTTCACAAATAAAAAGTGAAGCAGCTCAAGCTCATGCTGAGTTGCTTGAAGCAGAGGGCATAGTGCCTAAAAAGAAACATAGACGTGATGCTTTAAGAACAGAGCCTTATCGTGTGGATGTTGATGGCCGCATCTTTTGGAAATTAAAGGGTTACAACAATGGAGAAGATATCGTGCTTCAAGATTTGGGGGCCTGGGATGCAGTTGTATCTAAAGAAGAATGGTTTGTTTATGGTGCGGAGACAAAGGAAGCAATTGACAATTACTGTTCTTCTTTAAGGAGAAAAAAGTCTAGTGGAACCATGTCTCAAACTGTTCCTCGTGAAAGCGATGAAGAAAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

566

Amino Acids

63.44

Weight (kDa)

6.77

Isoelectric Point (pI)

43.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 10 - 68 2.9e-21 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1012
AciI CCGC 5 cut(s) 248, 886, 1273, 1483, 1592
AclWI GGATC 3 cut(s) 389, 422, 455
AcoI YGGCCR 2 cut(s) 883, 1480
AcsI RAATTY 6 cut(s) 223, 277, 310, 788, 818, 994
AcuI CTGAAG 1 cut(s) 72
AdeI CACNNNGTG 1 cut(s) 1051
AfiI CCNNNNNNNGG 5 cut(s) 93, 332, 446, 1012, 1054
AflIII ACRYGT 1 cut(s) 1695
AgsI TTSAA 8 cut(s) 315, 367, 572, 746, 823, 1211, 1403, 1535
AjiI CACGTC 1 cut(s) 1439
AjnI CCWGG 2 cut(s) 889, 1547
AjuI GAANNNNNNNTTGG 2 cut(s) 831, 863
AloI GAACNNNNNNTCC 2 cut(s) 164, 196
Alw21I GWGCWC 1 cut(s) 1324
Alw26I GTCTC 4 cut(s) 475, 635, 1589, 1665
AlwI GGATC 3 cut(s) 389, 422, 455
AoxI GGCC 7 cut(s) 133, 148, 300, 612, 883, 1480, 1545
ApeKI GCWGC 5 cut(s) 384, 417, 450, 1244, 1376
ApoI RAATTY 6 cut(s) 223, 277, 310, 788, 818, 994
Asp700I GAANNNNTTC 1 cut(s) 567
AspLEI GCGC 1 cut(s) 864
AspS9I GGNCC 2 cut(s) 215, 1545
AsuHPI GGTGA 3 cut(s) 572, 863, 1165
AvaII GGWCC 1 cut(s) 215
AxyI CCTNAGG 3 cut(s) 327, 711, 756
BauI CACGAG 1 cut(s) 1674
Bbv12I GWGCWC 1 cut(s) 1324
BbvI GCAGC 5 cut(s) 396, 429, 462, 1256, 1388
BccI CCATC 5 cut(s) 260, 407, 440, 971, 1472
BciT130I CCWGG 2 cut(s) 891, 1549
BclI TGATCA 1 cut(s) 286
BcoDI GTCTC 4 cut(s) 475, 635, 1589, 1665
BfaI CTAG 1 cut(s) 1647
BfmI CTRYAG 4 cut(s) 118, 941, 972, 1065
BglII AGATCT 1 cut(s) 1009
BisI GCNGC 7 cut(s) 385, 418, 451, 886, 1245, 1377, 1483
BlsI GCNGC 7 cut(s) 386, 419, 452, 887, 1246, 1378, 1484
Bme1390I CCNGG 2 cut(s) 891, 1549
Bme18I GGWCC 1 cut(s) 215
BmgBI CACGTC 1 cut(s) 1439
BmgT120I GGNCC 2 cut(s) 215, 1545
BmiI GGNNCC 4 cut(s) 963, 1004, 1546, 1654
BmrFI CCNGG 2 cut(s) 891, 1549
BmsI GCATC 6 cut(s) 535, 751, 1032, 1432, 1494, 1543
BplI GAGNNNNNCTC 2 cut(s) 798, 830
Bpu10I CCTNAGC 1 cut(s) 696
BpuEI CTTGAG 2 cut(s) 1253, 1365
BsaJI CCNNGG 7 cut(s) 168, 408, 441, 474, 615, 889, 1548
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 5 cut(s) 93, 332, 446, 1012, 1054
Bse1I ACTGG 2 cut(s) 80, 151
Bse21I CCTNAGG 3 cut(s) 327, 711, 756
BseBI CCWGG 2 cut(s) 891, 1549
BseDI CCNNGG 7 cut(s) 168, 408, 441, 474, 615, 889, 1548
BseLI CCNNNNNNNGG 5 cut(s) 93, 332, 446, 1012, 1054
BseMII CTCAG 9 cut(s) 318, 687, 702, 747, 1026, 1130, 1138, 1163, 1383
BseNI ACTGG 2 cut(s) 80, 151
BseRI GAGGAG 6 cut(s) 344, 892, 974, 1189, 1256, 1314
BseXI GCAGC 5 cut(s) 396, 429, 462, 1256, 1388
BshFI GGCC 7 cut(s) 135, 150, 302, 614, 885, 1482, 1547
BsiHKAI GWGCWC 1 cut(s) 1324
BsiSI CCGG 1 cut(s) 218
BslFI GGGAC 2 cut(s) 348, 422
BslI CCNNNNNNNGG 5 cut(s) 93, 332, 446, 1012, 1054
BsmAI GTCTC 4 cut(s) 475, 635, 1589, 1665
BsmFI GGGAC 2 cut(s) 348, 422
BsmI GAATGC 2 cut(s) 665, 717
BsnI GGCC 7 cut(s) 135, 150, 302, 614, 885, 1482, 1547
Bsp1286I GDGCHC 1 cut(s) 1324
Bsp143I GATC 6 cut(s) 286, 394, 427, 460, 523, 1009
BspACI CCGC 5 cut(s) 248, 886, 1273, 1483, 1592
BspANI GGCC 7 cut(s) 135, 150, 302, 614, 885, 1482, 1547
BspCNI CTCAG 9 cut(s) 319, 688, 703, 748, 1027, 1129, 1137, 1164, 1384
BspLI GGNNCC 4 cut(s) 963, 1004, 1546, 1654
BspMAI CTGCAG 1 cut(s) 122
BspPI GGATC 3 cut(s) 389, 422, 455
BsrI ACTGG 2 cut(s) 80, 151
BssECI CCNNGG 7 cut(s) 168, 408, 441, 474, 615, 889, 1548
BssMI GATC 6 cut(s) 286, 394, 427, 460, 523, 1009
BssSI CACGAG 1 cut(s) 1674
BssT1I CCWWGG 4 cut(s) 168, 441, 474, 615
Bst2BI CACGAG 1 cut(s) 1674
Bst2UI CCWGG 2 cut(s) 891, 1549
Bst4CI ACNGT 2 cut(s) 1622, 1669
Bst6I CTCTTC 2 cut(s) 48, 523
BstAPI GCANNNNNTGC 1 cut(s) 545
BstC8I GCNNGC 3 cut(s) 304, 879, 883
BstHHI GCGC 1 cut(s) 864
BstKTI GATC 6 cut(s) 289, 397, 430, 463, 526, 1012
BstMAI GTCTC 4 cut(s) 475, 635, 1589, 1665
BstMBI GATC 6 cut(s) 286, 394, 427, 460, 523, 1009
BstMWI GCNNNNNNNGC 6 cut(s) 545, 706, 770, 1279, 1382, 1553
BstNI CCWGG 2 cut(s) 891, 1549
BstNSI RCATGY 2 cut(s) 925, 1699
BstSCI CCNGG 2 cut(s) 889, 1547
BstSFI CTRYAG 4 cut(s) 118, 941, 972, 1065
BstV1I GCAGC 5 cut(s) 396, 429, 462, 1256, 1388
BstX2I RGATCY 1 cut(s) 1009
BstXI CCANNNNNNTGG 1 cut(s) 872
BstYI RGATCY 1 cut(s) 1009
Bsu36I CCTNAGG 3 cut(s) 327, 711, 756
BsuRI GGCC 7 cut(s) 135, 150, 302, 614, 885, 1482, 1547
BtrI CACGTC 1 cut(s) 1439
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 3 cut(s) 304, 879, 883
CfoI GCGC 1 cut(s) 864
Cfr13I GGNCC 2 cut(s) 215, 1545
CspCI CAANNNNNGTGG 2 cut(s) 157, 192
CviAII CATG 7 cut(s) 922, 999, 1196, 1343, 1388, 1657, 1696
DpnI GATC 6 cut(s) 288, 396, 429, 462, 525, 1011
DpnII GATC 6 cut(s) 286, 394, 427, 460, 523, 1009
DraIII CACNNNGTG 1 cut(s) 1051
EaeI YGGCCR 2 cut(s) 883, 1480
Eam1104I CTCTTC 2 cut(s) 48, 523
EarI CTCTTC 2 cut(s) 48, 523
Eco130I CCWWGG 4 cut(s) 168, 441, 474, 615
Eco147I AGGCCT 1 cut(s) 135
Eco32I GATATC 2 cut(s) 1102, 1525
Eco47I GGWCC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 72
Eco81I CCTNAGG 3 cut(s) 327, 711, 756
EcoO109I RGGNCCY 1 cut(s) 1545
EcoRI GAATTC 2 cut(s) 277, 310
EcoRII CCWGG 2 cut(s) 889, 1547
EcoRV GATATC 2 cut(s) 1102, 1525
EcoT14I CCWWGG 4 cut(s) 168, 441, 474, 615
EcoT22I ATGCAT 1 cut(s) 1025
ErhI CCWWGG 4 cut(s) 168, 441, 474, 615
FaeI CATG 7 cut(s) 925, 1002, 1199, 1346, 1391, 1660, 1699
FaqI GGGAC 2 cut(s) 348, 422
FatI CATG 7 cut(s) 921, 998, 1195, 1342, 1387, 1656, 1695
FbaI TGATCA 1 cut(s) 286
Fnu4HI GCNGC 7 cut(s) 385, 418, 451, 886, 1245, 1377, 1483
Fsp4HI GCNGC 7 cut(s) 385, 418, 451, 886, 1245, 1377, 1483
FspBI CTAG 1 cut(s) 1647
GlaI GCGC 1 cut(s) 863
GluI GCNGC 7 cut(s) 385, 418, 451, 886, 1245, 1377, 1483
HaeIII GGCC 7 cut(s) 135, 150, 302, 614, 885, 1482, 1547
HapII CCGG 1 cut(s) 218
HhaI GCGC 1 cut(s) 864
Hin1II CATG 7 cut(s) 925, 1002, 1199, 1346, 1391, 1660, 1699
Hin6I GCGC 1 cut(s) 862
HinP1I GCGC 1 cut(s) 862
HincII GTYRAC 1 cut(s) 204
HindII GTYRAC 1 cut(s) 204
HindIII AAGCTT 1 cut(s) 1298
HinfI GANTC 4 cut(s) 839, 1031, 1037, 1128
HpaII CCGG 1 cut(s) 218
HphI GGTGA 3 cut(s) 572, 863, 1165
Hpy166II GTNNAC 3 cut(s) 160, 204, 583
Hpy188I TCNGA 9 cut(s) 196, 409, 678, 1030, 1036, 1042, 1119, 1127, 1339
Hpy188III TCNNGA 7 cut(s) 326, 343, 504, 628, 755, 1535, 1676
Hpy8I GTNNAC 3 cut(s) 160, 204, 583
HpyAV CCTTC 7 cut(s) 40, 167, 235, 247, 361, 975, 1048
HpyCH4III ACNGT 2 cut(s) 1622, 1669
HpyCH4IV ACGT 1 cut(s) 1438
HpyF10VI GCNNNNNNNGC 6 cut(s) 545, 706, 770, 1279, 1382, 1553
HpySE526I ACGT 1 cut(s) 1438
Hsp92II CATG 7 cut(s) 925, 1002, 1199, 1346, 1391, 1660, 1699
HspAI GCGC 1 cut(s) 862
Ksp22I TGATCA 1 cut(s) 286
Kzo9I GATC 6 cut(s) 286, 394, 427, 460, 523, 1009
LmnI GCTCC 4 cut(s) 61, 961, 1176, 1327
Lsp1109I GCAGC 5 cut(s) 396, 429, 462, 1256, 1388
LweI GCATC 6 cut(s) 535, 751, 1032, 1432, 1494, 1543
MaeI CTAG 1 cut(s) 1647
MaeII ACGT 1 cut(s) 1438
MaeIII GTNAC 1 cut(s) 1505
MalI GATC 6 cut(s) 288, 396, 429, 462, 525, 1011
MboI GATC 6 cut(s) 286, 394, 427, 460, 523, 1009
MfeI CAATTG 1 cut(s) 1608
MflI RGATCY 1 cut(s) 1009
MhlI GDGCHC 1 cut(s) 1324
MlyI GAGTC 2 cut(s) 1046, 1122
Mph1103I ATGCAT 1 cut(s) 1025
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 7 cut(s) 597, 725, 1133, 1239, 1448, 1499, 1631
MslI CAYNNNNRTG 1 cut(s) 1437
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 2 cut(s) 891, 1549
MunI CAATTG 1 cut(s) 1608
Mva1269I GAATGC 2 cut(s) 665, 717
MvaI CCWGG 2 cut(s) 891, 1549
MwoI GCNNNNNNNGC 6 cut(s) 545, 706, 770, 1279, 1382, 1553
NdeII GATC 6 cut(s) 286, 394, 427, 460, 523, 1009
NlaIII CATG 7 cut(s) 925, 1002, 1199, 1346, 1391, 1660, 1699
NlaIV GGNNCC 4 cut(s) 963, 1004, 1546, 1654
NsiI ATGCAT 1 cut(s) 1025
NspI RCATGY 2 cut(s) 925, 1699
PceI AGGCCT 1 cut(s) 135
PciI ACATGT 1 cut(s) 1695
PcsI WCGNNNNNNNCGW 1 cut(s) 874
PctI GAATGC 2 cut(s) 665, 717
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 2 cut(s) 839, 1031
PflFI GACNNNGTC 1 cut(s) 623
PflMI CCANNNNNTGG 1 cut(s) 1012
PkrI GCNGC 7 cut(s) 386, 419, 452, 887, 1246, 1378, 1484
PleI GAGTC 2 cut(s) 1045, 1122
PpsI GAGTC 2 cut(s) 1045, 1122
PscI ACATGT 1 cut(s) 1695
Psp6I CCWGG 2 cut(s) 889, 1547
PspGI CCWGG 2 cut(s) 889, 1547
PspN4I GGNNCC 4 cut(s) 963, 1004, 1546, 1654
PspPI GGNCC 2 cut(s) 215, 1545
PstI CTGCAG 1 cut(s) 122
PsuI RGATCY 1 cut(s) 1009
PsyI GACNNNGTC 1 cut(s) 623
RseI CAYNNNNRTG 1 cut(s) 1437
SaqAI TTAA 7 cut(s) 597, 725, 1133, 1239, 1448, 1499, 1631
SatI GCNGC 7 cut(s) 385, 418, 451, 886, 1245, 1377, 1483
Sau3AI GATC 6 cut(s) 286, 394, 427, 460, 523, 1009
Sau96I GGNCC 2 cut(s) 215, 1545
SchI GAGTC 2 cut(s) 1046, 1122
ScrFI CCNGG 2 cut(s) 891, 1549
SduI GDGCHC 1 cut(s) 1324
SfaNI GCATC 6 cut(s) 535, 751, 1032, 1432, 1494, 1543
SfcI CTRYAG 4 cut(s) 118, 941, 972, 1065
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 1 cut(s) 1437
SmlI CTYRAG 2 cut(s) 1268, 1380
SmoI CTYRAG 2 cut(s) 1268, 1380
SseBI AGGCCT 1 cut(s) 135
SsiI CCGC 5 cut(s) 248, 886, 1273, 1483, 1592
SspI AATATT 1 cut(s) 673
SspMI CTAG 1 cut(s) 1647
StuI AGGCCT 1 cut(s) 135
StyD4I CCNGG 2 cut(s) 889, 1547
StyI CCWWGG 4 cut(s) 168, 441, 474, 615
TaaI ACNGT 2 cut(s) 1622, 1669
TaiI ACGT 1 cut(s) 1441
TaqI TCGA 2 cut(s) 264, 526
TauI GCSGC 2 cut(s) 888, 1485
TfiI GAWTC 2 cut(s) 839, 1031
Tru1I TTAA 7 cut(s) 597, 725, 1133, 1239, 1448, 1499, 1631
Tru9I TTAA 7 cut(s) 597, 725, 1133, 1239, 1448, 1499, 1631
TscAI CASTG 1 cut(s) 151
TseI GCWGC 5 cut(s) 384, 417, 450, 1244, 1376
TspDTI ATGAA 5 cut(s) 957, 987, 1212, 1359, 1701
TspGWI ACGGA 1 cut(s) 365
TspRI CASTG 1 cut(s) 151
Tth111I GACNNNGTC 1 cut(s) 623
Van91I CCANNNNNTGG 1 cut(s) 1012
VpaK11BI GGWCC 1 cut(s) 215
XapI RAATTY 6 cut(s) 223, 277, 310, 788, 818, 994
XceI RCATGY 2 cut(s) 925, 1699
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 1 cut(s) 1647
Zsp2I ATGCAT 1 cut(s) 1025
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.