pycom09g01060

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000989
Physical Location & Seq
Reverse (-)
850160 .. 851114
955 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g01060.4

Sequence Viewer

Length: 657 bp
ATGATGATGATGCCAAGGCCAAGGGGCAAGGCTGCAAGAGGGCGTCGCAGGGTCAAGGAACGCCACGTTGAGAGTAGAGAGGTTGAGCTTCCCCTACCTCAGGGCACCCCATTGACAACTGTAGCGGGTGTCAAATTAGCTGCTGAGGATGTGGGAAATACTCTCCAATTTTTGAAGTTTTGTGAAACTTTTAGAGAGTTTTTTAAGATTGAAAAGTCCCAAGCTCAGTCTGTTCTTCGGGAACTATCGCGTGGAGGAAGTGGGCGAAGTGGACGTCCTGGACAGTACTCCTTCATAGTTCGATTTCATAGCTATATGCTCTTCTTCATACAGAAGGGTTTATCACAGGAGTCTCTAGAGCAAGCAAGCAACAACTGGTCATGGTTCCAAGATTTGGCGAAGCTGGTGTGTGCATCGGGGGTCTCTAAGGTCGACCCTGTGATGAAGGAGCTGCCTGCAGAGTGTTTTATTGGAAGCAGGGAGGGATACAATGCCTTAAACTTCTCCCAAAATCTTAGGCTCCTGAATTTCATCTGTGCTGAGGCTCTTAACACAAAGACACTGAGGGGATGGATTGAGGAGCAAAATGAAAGATCTGTTCAATGTAAAGGGGACATGAAAGCAAAAGTTGCTCTAGCAAAGGATAAGGTAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

219

Amino Acids

24.68

Weight (kDa)

9.65

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 277
AccB1I GGYRCC 1 cut(s) 104
AccB7I CCANNNNNTGG 1 cut(s) 394
AccI GTMKAC 1 cut(s) 432
AccII CGCG 1 cut(s) 250
AciI CCGC 1 cut(s) 125
AcsI RAATTY 1 cut(s) 526
AcyI GRCGYC 2 cut(s) 43, 274
AfaI GTAC 1 cut(s) 287
AfiI CCNNNNNNNGG 2 cut(s) 100, 394
AgsI TTSAA 3 cut(s) 175, 212, 602
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 6 cut(s) 88, 140, 224, 312, 403, 451
AluI AGCT 6 cut(s) 88, 140, 224, 312, 403, 451
Alw26I GTCTC 2 cut(s) 357, 427
AoxI GGCC 1 cut(s) 17
ApeKI GCWGC 3 cut(s) 32, 140, 451
ApoI RAATTY 1 cut(s) 526
ArsI GACNNNNNNTTYG 2 cut(s) 259, 291
AxyI CCTNAGG 1 cut(s) 99
BaeGI GKGCMC 1 cut(s) 107
BanI GGYRCC 1 cut(s) 104
BbvCI CCTCAGC 2 cut(s) 144, 540
BbvI GCAGC 3 cut(s) 19, 127, 438
BccI CCATC 1 cut(s) 564
BciT130I CCWGG 1 cut(s) 279
BciVI GTATCC 1 cut(s) 479
BcoDI GTCTC 2 cut(s) 357, 427
BfaI CTAG 2 cut(s) 356, 635
BfmI CTRYAG 2 cut(s) 120, 456
BfuI GTATCC 1 cut(s) 479
BglII AGATCT 1 cut(s) 593
BisI GCNGC 3 cut(s) 33, 141, 452
BlsI GCNGC 3 cut(s) 34, 142, 453
BmcAI AGTACT 1 cut(s) 287
Bme1390I CCNGG 1 cut(s) 279
BmiI GGNNCC 3 cut(s) 106, 386, 521
BmrFI CCNGG 1 cut(s) 279
BmsI GCATC 1 cut(s) 422
Bpu10I CCTNAGC 2 cut(s) 144, 540
BsaHI GRCGYC 2 cut(s) 43, 274
BsaI GGTCTC 1 cut(s) 427
BsaJI CCNNGG 2 cut(s) 14, 20
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 394
Bse1I ACTGG 1 cut(s) 380
Bse21I CCTNAGG 1 cut(s) 99
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 14, 20
BseGI GGATG 2 cut(s) 154, 575
BseLI CCNNNNNNNGG 2 cut(s) 100, 394
BseMII CTCAG 5 cut(s) 113, 135, 239, 531, 554
BseNI ACTGG 1 cut(s) 380
BseRI GAGGAG 1 cut(s) 593
BseSI GKGCMC 1 cut(s) 107
BseXI GCAGC 3 cut(s) 19, 127, 438
Bsh1236I CGCG 1 cut(s) 250
BshFI GGCC 1 cut(s) 19
BshNI GGYRCC 1 cut(s) 104
BslFI GGGAC 2 cut(s) 202, 626
BslI CCNNNNNNNGG 2 cut(s) 100, 394
BsmAI GTCTC 2 cut(s) 357, 427
BsmFI GGGAC 2 cut(s) 202, 626
BsnI GGCC 1 cut(s) 19
Bso31I GGTCTC 1 cut(s) 427
Bsp1286I GDGCHC 1 cut(s) 107
Bsp143I GATC 1 cut(s) 593
BspACI CCGC 1 cut(s) 125
BspANI GGCC 1 cut(s) 19
BspCNI CTCAG 5 cut(s) 112, 136, 238, 532, 555
BspFNI CGCG 1 cut(s) 250
BspLI GGNNCC 3 cut(s) 106, 386, 521
BspMAI CTGCAG 1 cut(s) 460
BspQI GCTCTTC 1 cut(s) 326
BspT107I GGYRCC 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 427
BsrI ACTGG 1 cut(s) 380
BssECI CCNNGG 2 cut(s) 14, 20
BssMI GATC 1 cut(s) 593
BssNI GRCGYC 2 cut(s) 43, 274
BssT1I CCWWGG 2 cut(s) 14, 20
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 2 cut(s) 121, 285
Bst6I CTCTTC 1 cut(s) 326
BstACI GRCGYC 2 cut(s) 43, 274
BstAPI GCANNNNNTGC 1 cut(s) 629
BstC8I GCNNGC 3 cut(s) 363, 367, 456
BstDEI CTNAG 7 cut(s) 99, 144, 225, 426, 515, 540, 563
BstENI CCTNNNNNAGG 1 cut(s) 98
BstF5I GGATG 2 cut(s) 154, 575
BstFNI CGCG 1 cut(s) 250
BstKTI GATC 1 cut(s) 596
BstMAI GTCTC 2 cut(s) 357, 427
BstMBI GATC 1 cut(s) 593
BstMWI GCNNNNNNNGC 1 cut(s) 629
BstNI CCWGG 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 277
BstSFI CTRYAG 2 cut(s) 120, 456
BstSLI GKGCMC 1 cut(s) 107
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 3 cut(s) 19, 127, 438
BstX2I RGATCY 1 cut(s) 593
BstYI RGATCY 1 cut(s) 593
Bsu36I CCTNAGG 1 cut(s) 99
BsuI GTATCC 1 cut(s) 479
BsuRI GGCC 1 cut(s) 19
BtsCI GGATG 2 cut(s) 154, 575
BtsIMutI CAGTG 1 cut(s) 560
Cac8I GCNNGC 3 cut(s) 363, 367, 456
CseI GACGC 1 cut(s) 32
Csp6I GTAC 1 cut(s) 286
CviAII CATG 2 cut(s) 381, 616
CviQI GTAC 1 cut(s) 286
DdeI CTNAG 7 cut(s) 99, 144, 225, 426, 515, 540, 563
DpnI GATC 1 cut(s) 595
DpnII GATC 1 cut(s) 593
Eam1104I CTCTTC 1 cut(s) 326
EarI CTCTTC 1 cut(s) 326
Eco130I CCWWGG 2 cut(s) 14, 20
Eco31I GGTCTC 1 cut(s) 427
Eco81I CCTNAGG 1 cut(s) 99
EcoNI CCTNNNNNAGG 1 cut(s) 98
EcoRII CCWGG 1 cut(s) 277
EcoT14I CCWWGG 2 cut(s) 14, 20
ErhI CCWWGG 2 cut(s) 14, 20
FaeI CATG 2 cut(s) 384, 619
FaiI YATR 8 cut(s) 296, 309, 315, 317, 329, 382, 617, 655
FaqI GGGAC 2 cut(s) 202, 626
FatI CATG 2 cut(s) 380, 615
FauI CCCGC 1 cut(s) 118
FblI GTMKAC 1 cut(s) 432
Fnu4HI GCNGC 3 cut(s) 33, 141, 452
FokI GGATG 2 cut(s) 161, 582
Fsp4HI GCNGC 3 cut(s) 33, 141, 452
FspBI CTAG 2 cut(s) 356, 635
GluI GCNGC 3 cut(s) 33, 141, 452
HaeIII GGCC 1 cut(s) 19
HgaI GACGC 1 cut(s) 32
Hin1I GRCGYC 2 cut(s) 43, 274
Hin1II CATG 2 cut(s) 384, 619
HincII GTYRAC 1 cut(s) 433
HindII GTYRAC 1 cut(s) 433
HinfI GANTC 1 cut(s) 350
Hpy166II GTNNAC 2 cut(s) 272, 433
Hpy188III TCNNGA 3 cut(s) 239, 356, 523
Hpy8I GTNNAC 2 cut(s) 272, 433
Hpy99I CGWCG 1 cut(s) 48
HpyAV CCTTC 3 cut(s) 301, 328, 439
HpyCH4III ACNGT 2 cut(s) 121, 285
HpyCH4IV ACGT 2 cut(s) 66, 274
HpyCH4V TGCA 3 cut(s) 35, 413, 458
HpyF10VI GCNNNNNNNGC 1 cut(s) 629
HpyF3I CTNAG 7 cut(s) 99, 144, 225, 426, 515, 540, 563
HpySE526I ACGT 2 cut(s) 66, 274
Hsp92I GRCGYC 2 cut(s) 43, 274
Hsp92II CATG 2 cut(s) 384, 619
Kzo9I GATC 1 cut(s) 593
LguI GCTCTTC 1 cut(s) 326
LmnI GCTCC 3 cut(s) 448, 525, 580
Lsp1109I GCAGC 3 cut(s) 19, 127, 438
LweI GCATC 1 cut(s) 422
MaeI CTAG 2 cut(s) 356, 635
MaeII ACGT 2 cut(s) 66, 274
MalI GATC 1 cut(s) 595
MboI GATC 1 cut(s) 593
MboII GAAGA 3 cut(s) 227, 313, 316
MflI RGATCY 1 cut(s) 593
MhlI GDGCHC 1 cut(s) 107
MluCI AATT 3 cut(s) 134, 167, 526
MlyI GAGTC 1 cut(s) 359
MnlI CCTC 9 cut(s) 32, 73, 108, 139, 248, 475, 535, 558, 571
MseI TTAA 3 cut(s) 204, 497, 549
MspR9I CCNGG 1 cut(s) 279
MvaI CCWGG 1 cut(s) 279
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 1 cut(s) 629
NdeII GATC 1 cut(s) 593
NlaIII CATG 2 cut(s) 384, 619
NlaIV GGNNCC 3 cut(s) 106, 386, 521
PciSI GCTCTTC 1 cut(s) 326
PflMI CCANNNNNTGG 1 cut(s) 394
PfoI TCCNGGA 1 cut(s) 277
PkrI GCNGC 3 cut(s) 34, 142, 453
PleI GAGTC 1 cut(s) 358
PpsI GAGTC 1 cut(s) 358
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
PspN4I GGNNCC 3 cut(s) 106, 386, 521
PstI CTGCAG 1 cut(s) 460
PsuI RGATCY 1 cut(s) 593
RsaI GTAC 1 cut(s) 287
RsaNI GTAC 1 cut(s) 286
SalI GTCGAC 1 cut(s) 431
SapI GCTCTTC 1 cut(s) 326
SaqAI TTAA 3 cut(s) 204, 497, 549
SatI GCNGC 3 cut(s) 33, 141, 452
Sau3AI GATC 1 cut(s) 593
ScaI AGTACT 1 cut(s) 287
SchI GAGTC 1 cut(s) 359
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 1 cut(s) 107
SfaNI GCATC 1 cut(s) 422
SfcI CTRYAG 2 cut(s) 120, 456
Sse9I AATT 3 cut(s) 134, 167, 526
SsiI CCGC 1 cut(s) 125
SspMI CTAG 2 cut(s) 356, 635
StyD4I CCNGG 1 cut(s) 277
StyI CCWWGG 2 cut(s) 14, 20
TaaI ACNGT 2 cut(s) 121, 285
TaiI ACGT 2 cut(s) 69, 277
TaqI TCGA 2 cut(s) 301, 432
TasI AATT 3 cut(s) 134, 167, 526
TatI WGTACW 1 cut(s) 285
Tru1I TTAA 3 cut(s) 204, 497, 549
Tru9I TTAA 3 cut(s) 204, 497, 549
TscAI CASTG 1 cut(s) 567
TseI GCWGC 3 cut(s) 32, 140, 451
TspDTI ATGAA 7 cut(s) 283, 296, 316, 458, 520, 603, 632
TspRI CASTG 1 cut(s) 567
Van91I CCANNNNNTGG 1 cut(s) 394
XagI CCTNNNNNAGG 1 cut(s) 98
XapI RAATTY 1 cut(s) 526
XbaI TCTAGA 1 cut(s) 355
XmiI GTMKAC 1 cut(s) 432
XspI CTAG 2 cut(s) 356, 635
ZraI GACGTC 1 cut(s) 275
ZrmI AGTACT 1 cut(s) 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.