RLG00000021374

WSTF, HB1, Itc1p, MBD9 motif 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000989
Physical Location & Seq
Forward (+)
74672826 .. 74675887
3062 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021374

Sequence Viewer

Length: 1797 bp
ATGGCCGGAGTTCGTGTCGTCGGTGGCCGGATCTACGATTCCGAGAACGGAAAAACCTGTCACCAGTGCCGGCAGAAGACTAGGGATTTTGTGGCGTCGTGTAAGAATCTGAAGAAGAATGCTCGCTGCACCATTAGCTTTTGTCATAAATGCCTCTTGAACAGATATGGAGAGAAGGCTGAAGAGGTGGAGCTTTTGGAGGACTGGAATTGCCCCAAATGTAGGGGGAATTGTAATTGTAGTTTCTGCAGGAAGAAACAAGGCCTCAAACCCACTGGTCTTCTTGTTCACACGGCCAAGGAAGGTGGGTTTGGTTCGGTTTCAGAGATGTTGACTGTGAGAGGACCCGAGAATTTTGGTATTGAGAGGAAGGTTGCAGAGAAGGTGGCTGTCGATGAAAAGGAGAATTCTGTTGATCAAAAGGTTGAGGCTAGCCTGAATTCAACACAAAATCCTGAGGAGGGAGAAGTCAAGAAAACAAAGAGGAAAGGATTGAAGGAAATCCGTAATGGCAGCAGGGATGATCCTAAAGTCTCCGAGGATGGCAGCAGGGATGATCCTAAAGTCTCCAAGGATGTTGCTGTAAAAGAAGTGAAATCAAGGAAGAGAAAAAATAAAGATCGAAGAGAAGAAGCAGATGATGCGAAACTGCAAGGTGAAGAAGTTCAAAATGTTGTGAACAACAATGATGTCAAGAGGAAGAAAAAGGCCAAGGACAAGGTCAAGAGTAGCAAAGAGACTTGCAGAGTTGAGGAATGCACCAAGAATATTCTGAACAAAAAAGTTGAGCCTGAGCTTCCCTTGCCTGAGGGCATTCCCTTAACAAGTGTCTTGGGAATTGAACTGCCTCCTGAGGATGCTGGAAACGCTCTGCAATTTTTGGAATTTTGTGCAGCTTTTGGAGAGGTTCTCAAATTCAAAAAAGCACAGGCTGAATCTATACTTGGTGAATTACTGCGCCAACGAAGTGGGCGAGCTGGCCGCCCTGGACAATACTCCTCACTTATTCGGTTTCACATGCAACTATTATCTCTACTACAGGAGGATATGGGAGAGGAGCCTTCATCTATAGATGAAATGAGCCACAAAAATTCATGGTTCCAAGATGTGGGTAAATGCATCTCTGAATCTGAGTCCGAACACCTATTGAAGGAACTGCCTACAGATTGTTTCAGTAGAGGTGGTGCTGGATATGATATCTTAGACCTCTCTCAGAAGCTCAGACTCATAACTTACCTTTGTGATGAGGTGATTTGCACTTCTAAACTGAGGAGCTGGATAGAAGAACAGCATGAAAAGATTGTTGAAAGCGAAAAGGAAGCAAAAGGAAAAGTTAATGCAGCCAAAGATAAGGAAAAACTCCTCAAGCGGAAGTTGCAAGATGAGGTGGCGAAGCTTATTATTGCAAAAAATGGTGCTCCTCTGTCAATTTCAGAACATGAAGCTCTTGTTTCACAAATAAAAAGTGAAGCAGCTCAAGCTCATGCTGAGTTGCTTGAAGCAGAGGGCATAGTGCCTAAAAAGAAACATAGATGTGATGCTTTAAGAACGGAGCCTTATCGTGTGGATGTTGATGGCCGCATCTTTTGGAAATTAAAGGGTTACAACAATGGAGAAGATATCGTGCTTCAAGATTTGGGGGCCTGGGATGCAGTTGTATCTAAAGAAGAATGGTTTGTTTATGGTGCGGAGACAAAGGAAGCAATTGACAGTTACTGTTCTTCTTTAAGGAGAAAAAAGACTAGTGGAACCATGTCACAAACTGTTCCTCGTGAAAGCGATGAAGAAAACATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT WHIM1 zf-4CXXC_R1

Protein Analysis

599

Amino Acids

67.21

Weight (kDa)

8.09

Isoelectric Point (pI)

43.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-4CXXC_R1 PF10497 12 - 111 1.7e-36 Zinc-finger domain of monoamine-oxidase A repressor R1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1108
AciI CCGC 4 cut(s) 982, 1369, 1579, 1688
AclWI GGATC 3 cut(s) 38, 518, 551
AcoI YGGCCR 5 cut(s) 3, 25, 294, 979, 1576
AcsI RAATTY 6 cut(s) 352, 406, 439, 884, 914, 1090
AcuI CTGAAG 2 cut(s) 131, 201
AcyI GRCGYC 1 cut(s) 95
AfiI CCNNNNNNNGG 4 cut(s) 222, 461, 1108, 1150
AflIII ACRYGT 1 cut(s) 1791
AhlI ACTAGT 1 cut(s) 1742
AjnI CCWGG 2 cut(s) 985, 1643
AjuI GAANNNNNNNTTGG 4 cut(s) 294, 326, 927, 959
Alw21I GWGCWC 1 cut(s) 1420
Alw26I GTCTC 4 cut(s) 538, 571, 731, 1685
AlwI GGATC 3 cut(s) 38, 518, 551
AlwNI CAGNNNCTG 1 cut(s) 1716
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 8 cut(s) 3, 25, 262, 294, 708, 979, 1576, 1641
ApeKI GCWGC 6 cut(s) 126, 513, 546, 893, 1340, 1472
ApoI RAATTY 6 cut(s) 352, 406, 439, 884, 914, 1090
Asp700I GAANNNNTTC 1 cut(s) 663
AspLEI GCGC 1 cut(s) 960
AspS9I GGNCC 2 cut(s) 344, 1641
AsuHPI GGTGA 4 cut(s) 53, 668, 959, 1261
AsuNHI GCTAGC 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 1 cut(s) 344
AxyI CCTNAGG 3 cut(s) 456, 807, 852
BauI CACGAG 1 cut(s) 1770
BbsI GAAGAC 2 cut(s) 83, 272
Bbv12I GWGCWC 1 cut(s) 1420
BbvI GCAGC 6 cut(s) 113, 525, 558, 905, 1352, 1484
BccI CCATC 2 cut(s) 536, 1568
BceAI ACGGC 1 cut(s) 309
BciT130I CCWGG 2 cut(s) 987, 1645
BclI TGATCA 1 cut(s) 415
BcoDI GTCTC 4 cut(s) 538, 571, 731, 1685
BcuI ACTAGT 1 cut(s) 1742
BfaI CTAG 3 cut(s) 81, 432, 1743
BfmI CTRYAG 4 cut(s) 247, 1037, 1068, 1161
BisI GCNGC 8 cut(s) 127, 514, 547, 894, 982, 1341, 1473, 1579
BlsI GCNGC 8 cut(s) 128, 515, 548, 895, 983, 1342, 1474, 1580
Bme1390I CCNGG 2 cut(s) 987, 1645
Bme18I GGWCC 1 cut(s) 344
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 2 cut(s) 344, 1641
BmiI GGNNCC 6 cut(s) 346, 1059, 1100, 1554, 1642, 1750
BmrFI CCNGG 2 cut(s) 987, 1645
BmsI GCATC 6 cut(s) 631, 847, 1128, 1528, 1590, 1639
BmtI GCTAGC 1 cut(s) 435
BpiI GAAGAC 2 cut(s) 83, 272
BplI GAGNNNNNCTC 2 cut(s) 894, 926
Bpu10I CCTNAGC 1 cut(s) 792
BpuEI CTTGAG 2 cut(s) 1349, 1461
BsaHI GRCGYC 1 cut(s) 95
BsaJI CCNNGG 6 cut(s) 297, 537, 570, 711, 985, 1644
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 4 cut(s) 222, 461, 1108, 1150
Bse118I RCCGGY 1 cut(s) 69
Bse1I ACTGG 3 cut(s) 64, 209, 280
Bse21I CCTNAGG 3 cut(s) 456, 807, 852
BseBI CCWGG 2 cut(s) 987, 1645
BseDI CCNNGG 6 cut(s) 297, 537, 570, 711, 985, 1644
BseGI GGATG 7 cut(s) 526, 547, 559, 580, 862, 1573, 1654
BseLI CCNNNNNNNGG 4 cut(s) 222, 461, 1108, 1150
BseMII CTCAG 9 cut(s) 447, 783, 798, 843, 1122, 1226, 1234, 1259, 1479
BseNI ACTGG 3 cut(s) 64, 209, 280
BseRI GAGGAG 6 cut(s) 473, 988, 1070, 1285, 1352, 1410
BseXI GCAGC 6 cut(s) 113, 525, 558, 905, 1352, 1484
BsgI GTGCAG 2 cut(s) 112, 912
BshFI GGCC 8 cut(s) 5, 27, 264, 296, 710, 981, 1578, 1643
BsiHKAI GWGCWC 1 cut(s) 1420
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 3 cut(s) 6, 28, 70
BslI CCNNNNNNNGG 4 cut(s) 222, 461, 1108, 1150
BsmAI GTCTC 4 cut(s) 538, 571, 731, 1685
BsmI GAATGC 3 cut(s) 124, 761, 813
BsnI GGCC 8 cut(s) 5, 27, 264, 296, 710, 981, 1578, 1643
BsoBI CYCGRG 1 cut(s) 347
Bsp1286I GDGCHC 1 cut(s) 1420
Bsp143I GATC 5 cut(s) 30, 415, 523, 556, 619
BspACI CCGC 4 cut(s) 982, 1369, 1579, 1688
BspANI GGCC 8 cut(s) 5, 27, 264, 296, 710, 981, 1578, 1643
BspCNI CTCAG 9 cut(s) 448, 784, 799, 844, 1123, 1225, 1233, 1260, 1480
BspLI GGNNCC 6 cut(s) 346, 1059, 1100, 1554, 1642, 1750
BspMAI CTGCAG 1 cut(s) 251
BspOI GCTAGC 1 cut(s) 435
BspPI GGATC 3 cut(s) 38, 518, 551
BsrFI RCCGGY 1 cut(s) 69
BsrI ACTGG 3 cut(s) 64, 209, 280
BssAI RCCGGY 1 cut(s) 69
BssECI CCNNGG 6 cut(s) 297, 537, 570, 711, 985, 1644
BssMI GATC 5 cut(s) 30, 415, 523, 556, 619
BssNI GRCGYC 1 cut(s) 95
BssSI CACGAG 1 cut(s) 1770
BssT1I CCWWGG 3 cut(s) 297, 570, 711
Bst2BI CACGAG 1 cut(s) 1770
Bst2UI CCWGG 2 cut(s) 987, 1645
Bst4CI ACNGT 4 cut(s) 337, 1712, 1718, 1765
Bst6I CTCTTC 3 cut(s) 177, 599, 619
BstACI GRCGYC 1 cut(s) 95
BstAPI GCANNNNNTGC 1 cut(s) 641
BstC8I GCNNGC 5 cut(s) 71, 124, 433, 975, 979
BstENI CCTNNNNNAGG 1 cut(s) 1148
BstF5I GGATG 7 cut(s) 526, 547, 559, 580, 862, 1573, 1654
BstHHI GCGC 1 cut(s) 960
BstKTI GATC 5 cut(s) 33, 418, 526, 559, 622
BstMAI GTCTC 4 cut(s) 538, 571, 731, 1685
BstMBI GATC 5 cut(s) 30, 415, 523, 556, 619
BstMWI GCNNNNNNNGC 7 cut(s) 135, 641, 802, 866, 1375, 1478, 1649
BstNI CCWGG 2 cut(s) 987, 1645
BstNSI RCATGY 2 cut(s) 1021, 1795
BstSCI CCNGG 2 cut(s) 985, 1643
BstSFI CTRYAG 4 cut(s) 247, 1037, 1068, 1161
BstV1I GCAGC 6 cut(s) 113, 525, 558, 905, 1352, 1484
BstV2I GAAGAC 2 cut(s) 83, 272
BstX2I RGATCY 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 968
BstYI RGATCY 1 cut(s) 30
Bsu36I CCTNAGG 3 cut(s) 456, 807, 852
BsuRI GGCC 8 cut(s) 5, 27, 264, 296, 710, 981, 1578, 1643
BtsCI GGATG 7 cut(s) 526, 547, 559, 580, 862, 1573, 1654
BtsIMutI CAGTG 2 cut(s) 71, 273
Cac8I GCNNGC 5 cut(s) 71, 124, 433, 975, 979
CaiI CAGNNNCTG 1 cut(s) 1716
CfoI GCGC 1 cut(s) 960
Cfr10I RCCGGY 1 cut(s) 69
Cfr13I GGNCC 2 cut(s) 344, 1641
CseI GACGC 1 cut(s) 84
CspCI CAANNNNNGTGG 2 cut(s) 286, 321
CviAII CATG 7 cut(s) 1018, 1095, 1292, 1439, 1484, 1753, 1792
DpnI GATC 5 cut(s) 32, 417, 525, 558, 621
DpnII GATC 5 cut(s) 30, 415, 523, 556, 619
EaeI YGGCCR 5 cut(s) 3, 25, 294, 979, 1576
Eam1104I CTCTTC 3 cut(s) 177, 599, 619
EarI CTCTTC 3 cut(s) 177, 599, 619
Eco130I CCWWGG 3 cut(s) 297, 570, 711
Eco147I AGGCCT 1 cut(s) 264
Eco32I GATATC 2 cut(s) 1198, 1621
Eco47I GGWCC 1 cut(s) 344
Eco57I CTGAAG 2 cut(s) 131, 201
Eco81I CCTNAGG 3 cut(s) 456, 807, 852
Eco88I CYCGRG 1 cut(s) 347
EcoNI CCTNNNNNAGG 1 cut(s) 1148
EcoO109I RGGNCCY 2 cut(s) 344, 1641
EcoRI GAATTC 2 cut(s) 406, 439
EcoRII CCWGG 2 cut(s) 985, 1643
EcoRV GATATC 2 cut(s) 1198, 1621
EcoT14I CCWWGG 3 cut(s) 297, 570, 711
EcoT22I ATGCAT 1 cut(s) 1121
ErhI CCWWGG 3 cut(s) 297, 570, 711
FaeI CATG 7 cut(s) 1021, 1098, 1295, 1442, 1487, 1756, 1795
FatI CATG 7 cut(s) 1017, 1094, 1291, 1438, 1483, 1752, 1791
FbaI TGATCA 1 cut(s) 415
Fnu4HI GCNGC 8 cut(s) 127, 514, 547, 894, 982, 1341, 1473, 1579
FokI GGATG 7 cut(s) 533, 554, 566, 587, 869, 1580, 1661
Fsp4HI GCNGC 8 cut(s) 127, 514, 547, 894, 982, 1341, 1473, 1579
FspBI CTAG 3 cut(s) 81, 432, 1743
GlaI GCGC 1 cut(s) 959
GluI GCNGC 8 cut(s) 127, 514, 547, 894, 982, 1341, 1473, 1579
HaeIII GGCC 8 cut(s) 5, 27, 264, 296, 710, 981, 1578, 1643
HapII CCGG 3 cut(s) 6, 28, 70
HgaI GACGC 1 cut(s) 84
HhaI GCGC 1 cut(s) 960
Hin1I GRCGYC 1 cut(s) 95
Hin1II CATG 7 cut(s) 1021, 1098, 1295, 1442, 1487, 1756, 1795
Hin6I GCGC 1 cut(s) 958
HinP1I GCGC 1 cut(s) 958
HincII GTYRAC 1 cut(s) 333
HindII GTYRAC 1 cut(s) 333
HindIII AAGCTT 1 cut(s) 1394
HinfI GANTC 6 cut(s) 38, 106, 935, 1127, 1133, 1224
HpaII CCGG 3 cut(s) 6, 28, 70
HphI GGTGA 4 cut(s) 53, 668, 959, 1261
Hpy166II GTNNAC 3 cut(s) 289, 333, 679
Hpy188III TCNNGA 8 cut(s) 157, 455, 472, 694, 724, 851, 1631, 1772
Hpy8I GTNNAC 3 cut(s) 289, 333, 679
Hpy99I CGWCG 2 cut(s) 23, 100
HpyAV CCTTC 7 cut(s) 169, 296, 364, 376, 490, 1071, 1144
HpyCH4III ACNGT 4 cut(s) 337, 1712, 1718, 1765
HpyF10VI GCNNNNNNNGC 7 cut(s) 135, 641, 802, 866, 1375, 1478, 1649
Hsp92I GRCGYC 1 cut(s) 95
Hsp92II CATG 7 cut(s) 1021, 1098, 1295, 1442, 1487, 1756, 1795
HspAI GCGC 1 cut(s) 958
KroI GCCGGC 1 cut(s) 69
KroNI GCCGGC 1 cut(s) 71
Ksp22I TGATCA 1 cut(s) 415
Kzo9I GATC 5 cut(s) 30, 415, 523, 556, 619
LmnI GCTCC 5 cut(s) 190, 1057, 1272, 1423, 1552
Lsp1109I GCAGC 6 cut(s) 113, 525, 558, 905, 1352, 1484
LweI GCATC 6 cut(s) 631, 847, 1128, 1528, 1590, 1639
MaeI CTAG 3 cut(s) 81, 432, 1743
MaeIII GTNAC 4 cut(s) 59, 1601, 1712, 1755
MalI GATC 5 cut(s) 32, 417, 525, 558, 621
MboI GATC 5 cut(s) 30, 415, 523, 556, 619
MfeI CAATTG 1 cut(s) 1704
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 1420
MlyI GAGTC 2 cut(s) 1142, 1218
Mph1103I ATGCAT 1 cut(s) 1121
MroNI GCCGGC 1 cut(s) 69
MroXI GAANNNNTTC 1 cut(s) 663
MseI TTAA 5 cut(s) 821, 1335, 1544, 1595, 1727
MslI CAYNNNNRTG 1 cut(s) 1533
MspI CCGG 3 cut(s) 6, 28, 70
MspR9I CCNGG 2 cut(s) 987, 1645
MunI CAATTG 1 cut(s) 1704
Mva1269I GAATGC 3 cut(s) 124, 761, 813
MvaI CCWGG 2 cut(s) 987, 1645
MwoI GCNNNNNNNGC 7 cut(s) 135, 641, 802, 866, 1375, 1478, 1649
NaeI GCCGGC 1 cut(s) 71
NdeII GATC 5 cut(s) 30, 415, 523, 556, 619
NgoMIV GCCGGC 1 cut(s) 69
NheI GCTAGC 1 cut(s) 431
NlaIII CATG 7 cut(s) 1021, 1098, 1295, 1442, 1487, 1756, 1795
NlaIV GGNNCC 6 cut(s) 346, 1059, 1100, 1554, 1642, 1750
NmuCI GTSAC 2 cut(s) 59, 1755
NsiI ATGCAT 1 cut(s) 1121
NspI RCATGY 2 cut(s) 1021, 1795
PceI AGGCCT 1 cut(s) 264
PciI ACATGT 1 cut(s) 1791
PcsI WCGNNNNNNNCGW 1 cut(s) 970
PctI GAATGC 3 cut(s) 124, 761, 813
PdiI GCCGGC 1 cut(s) 71
PdmI GAANNNNTTC 1 cut(s) 663
PfeI GAWTC 4 cut(s) 38, 106, 935, 1127
PflFI GACNNNGTC 1 cut(s) 719
PflMI CCANNNNNTGG 1 cut(s) 1108
PkrI GCNGC 8 cut(s) 128, 515, 548, 895, 983, 1342, 1474, 1580
PleI GAGTC 2 cut(s) 1141, 1218
PpsI GAGTC 2 cut(s) 1141, 1218
PpuMI RGGWCCY 1 cut(s) 344
PscI ACATGT 1 cut(s) 1791
Psp5II RGGWCCY 1 cut(s) 344
Psp6I CCWGG 2 cut(s) 985, 1643
PspGI CCWGG 2 cut(s) 985, 1643
PspN4I GGNNCC 6 cut(s) 346, 1059, 1100, 1554, 1642, 1750
PspPI GGNCC 2 cut(s) 344, 1641
PspPPI RGGWCCY 1 cut(s) 344
PstI CTGCAG 1 cut(s) 251
PstNI CAGNNNCTG 1 cut(s) 1716
PsuI RGATCY 1 cut(s) 30
PsyI GACNNNGTC 1 cut(s) 719
RseI CAYNNNNRTG 1 cut(s) 1533
SaqAI TTAA 5 cut(s) 821, 1335, 1544, 1595, 1727
SatI GCNGC 8 cut(s) 127, 514, 547, 894, 982, 1341, 1473, 1579
Sau3AI GATC 5 cut(s) 30, 415, 523, 556, 619
Sau96I GGNCC 2 cut(s) 344, 1641
SchI GAGTC 2 cut(s) 1142, 1218
ScrFI CCNGG 2 cut(s) 987, 1645
SduI GDGCHC 1 cut(s) 1420
SfaNI GCATC 6 cut(s) 631, 847, 1128, 1528, 1590, 1639
SfcI CTRYAG 4 cut(s) 247, 1037, 1068, 1161
SinI GGWCC 1 cut(s) 344
SmiMI CAYNNNNRTG 1 cut(s) 1533
SmlI CTYRAG 2 cut(s) 1364, 1476
SmoI CTYRAG 2 cut(s) 1364, 1476
SpeI ACTAGT 1 cut(s) 1742
SseBI AGGCCT 1 cut(s) 264
SsiI CCGC 4 cut(s) 982, 1369, 1579, 1688
SspI AATATT 1 cut(s) 769
SspMI CTAG 3 cut(s) 81, 432, 1743
StuI AGGCCT 1 cut(s) 264
StyD4I CCNGG 2 cut(s) 985, 1643
StyI CCWWGG 3 cut(s) 297, 570, 711
TaaI ACNGT 4 cut(s) 337, 1712, 1718, 1765
TaqI TCGA 2 cut(s) 393, 622
TauI GCSGC 2 cut(s) 984, 1581
TfiI GAWTC 4 cut(s) 38, 106, 935, 1127
Tru1I TTAA 5 cut(s) 821, 1335, 1544, 1595, 1727
Tru9I TTAA 5 cut(s) 821, 1335, 1544, 1595, 1727
TscAI CASTG 2 cut(s) 71, 280
TseFI GTSAC 2 cut(s) 59, 1755
TseI GCWGC 6 cut(s) 126, 513, 546, 893, 1340, 1472
Tsp45I GTSAC 2 cut(s) 59, 1755
TspDTI ATGAA 7 cut(s) 411, 1053, 1083, 1089, 1308, 1455, 1797
TspGWI ACGGA 3 cut(s) 63, 494, 1565
TspRI CASTG 2 cut(s) 71, 280
Tth111I GACNNNGTC 1 cut(s) 719
Van91I CCANNNNNTGG 1 cut(s) 1108
VpaK11BI GGWCC 1 cut(s) 344
XagI CCTNNNNNAGG 1 cut(s) 1148
XapI RAATTY 6 cut(s) 352, 406, 439, 884, 914, 1090
XceI RCATGY 2 cut(s) 1021, 1795
XmnI GAANNNNTTC 1 cut(s) 663
XspI CTAG 3 cut(s) 81, 432, 1743
Zsp2I ATGCAT 1 cut(s) 1121
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.